• Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. (wikipedia.org)
  • Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. (wikipedia.org)
  • Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. (lookformedical.com)
  • The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
  • Folding and stability of the isolated Greek key domains of the long-lived human lens proteins gammaD-crystallin and gammaS-crystallin. (protabank.org)
  • The transparency of the eye lens depends on the high solubility and stability of the lens crystallin proteins. (protabank.org)
  • α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
  • To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
  • CRYAA is one of the major lens proteins, accounting for 35% of all crystallins. (molvis.org)
  • This gene encodes a member of the beta/gamma-crystallin family of proteins. (raybiotech.com)
  • Crystallins constitute the major proteins of vertebrate eye lens and maintain the transparency and refractive index of the lens. (raybiotech.com)
  • For example, cystic fibrosis is caused by a defective cystic fibrosis transmembrane conductance regulator (CFTR) protein, [3] and in amyotrophic lateral sclerosis / frontotemporal lobar degeneration (FTLD), certain gene-regulating proteins inappropriately aggregate in the cytoplasm, and thus are unable to perform their normal tasks within the nucleus. (wikipedia.org)
  • The following CRYGS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • Macaca mulatta crystallin gamma S (CRYGS), mRNA. (genscript.com)
  • Gamma-crystallin D is a protein that in humans is encoded by the CRYGD gene. (wikipedia.org)
  • Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. (wikipedia.org)
  • Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a betagamma-Crystallin Protein. (unige.ch)
  • Stability, homodimerization, and calcium-binding properties of a single, variant betagamma-crystallin domain of the protein absent in melanoma 1 (AIM1). (unige.ch)
  • The decrease in stability of the N-terminal domain of human gammaD-crystallin with respect to the complete protein indicated that the interdomain interface contributes of 4.2 kcal/mol to the overall stability of this very long-lived protein. (protabank.org)
  • HspX or α-crystallin, a 16 kDa protein, is one of the dominant antigens expressed during the latent stages of M. tuberculosis infection and under various other conditions like low oxygen tension, nutrient starvation or hypoxia 7 , 8 . (nature.com)
  • The scientist's investigation covers issues in Molecular biology, Cell biology, Crystallin, Heat shock protein and Alpha-Crystallin A Chain. (research.com)
  • His Cell biology research incorporates elements of Heat shock protein, Alpha-Crystallin A Chain, Apoptosis, Lens and Genetically modified mouse. (research.com)
  • Specifically, gene programs controlling carbohydrate and protein absorption were c-Maf-dependent. (bvsalud.org)
  • There have been many genes identified as TH response genes such as sonic hedgehog (Shh), bone morphogenetic protein-4 (BMP-4), matrix metalloproteinases (MMPs) and so on. (wixsite.com)
  • You are hearing Gamma Crystallin Music , a representation of the amino acid sequence of a lens protein. (whozoo.org)
  • 1 In the King lab, I focused on the aggregation pathways of oxidation-mimicking Trp variants of human γD-crystallin, 2 revealing another unusual phenomenon: the highly stable, aggregation-resistant WT protein drove a destabilized but still well folded variant into an aggregated state without itself aggregating 3 ( Figure 1 ). (mit.edu)
  • Most recently, as a postdoc in the Shakhnovich group at Harvard, I drew inspiration from the disulfide chemistry of the lens crystallins to devise a high-throughput disulfide scanning (HTDS) methodology that aims to systematically discover protein conformations, including non-native conformations, and link them to phenotypic effects. (mit.edu)
  • In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
  • All six exons of crystallin, beta A4 gene ( CRYBA4 ) were amplified by PCR methods and direct sequencing. (molvis.org)
  • Beta-crystallin A4 (CRYBA4) is a member of the beta/gamma-crystallin family which are the dominant structural components of the vertebrate eye lens. (prospecbio.com)
  • The CRYBA4 gene being a beta acidic group member is part of a gene cluster with beta-B1, beta-B2, and beta-B3. (prospecbio.com)
  • Lists of genes constitutively expressed or silent during myelopoiesis and of genes differentially expressed in commitment phase of myelopoiesis were first identified using a classical data analysis procedure. (biomedcentral.com)
  • This approach allowed identifying specific chromosomal regions significantly highly or weakly expressed, and clusters of differentially expressed genes and of transcripts related to specific functional modules. (biomedcentral.com)
  • 600 genes differentially expressed during Leber congenital amaurosis_Retina_GSE3249 disease perturbation from the GEO Signatures of Differentially Expressed Genes for Diseases dataset. (maayanlab.cloud)
  • Isolation and characterization of genes associated with chromosome-6 mediated tumor suppression in human malignant melanoma. (unige.ch)
  • Each of these strings consists of the intertwined names of two human genes (standard names can be found at http://www.genenames.org/ ), and each pair of genes is located on the same human chromosome. (mit.edu)
  • This gene and several family members are present in a gene cluster on chromosome 2. (raybiotech.com)
  • Entrez Gene: CRYGD crystallin, gamma D". Graw J (1998). (wikipedia.org)
  • Conversion and compensatory evolution of the gamma-crystallin genes and identification of a cataractogenic mutation that reverses the sequence of the human CRYGD gene to an ancestral state. (umassmed.edu)
  • Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. (wikipedia.org)
  • Lee and Sonnhammer [ 11 ] identified a significant tendency to cluster of genes encoding products active in the same pathway. (biomedcentral.com)
  • This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B2. (oftalmic.ru)
  • Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). (oftalmic.ru)
  • The dose-dependent induction of FLOWERING LOCUS T-like genes ( FTLs ) may have contributed to dose-dependent flowering induction in pineapple by ethylene. (nature.com)
  • T cell receptor gamma locus antisense. (gsea-msigdb.org)
  • This record is derived from a genomic sequence (NC_041755.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. (genscript.com)
  • This study aimed at investigating the genomic expression during myeloid differentiation through a computational approach that integrates gene expression profiles with functional information and genome organization. (biomedcentral.com)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • Then, the genomic distribution of myelopoiesis genes was investigated integrating transcriptional and functional characteristics of genes. (biomedcentral.com)
  • The analysis of genomic expression during human myelopoiesis using an integrative computational approach allowed discovering important relationships between genomic position, biological function and expression patterns and highlighting chromatin domains, including genes with coordinated expression and lineage-specific functions. (biomedcentral.com)
  • In particular, several genomic studies suggested the existence of relationships between gene expression and genomic position. (biomedcentral.com)
  • The analysis of transcriptome data in the perspective of the genomic organization of genes could certainly shed light not only on the aberrations leading to pathological states but also on the physiological mechanisms of all cellular processes, including cellular differentiation. (biomedcentral.com)
  • Transposon vectors can be used in the frog for transgenesis and for insertional mutagenesis where enhancer trap and gene trap constructs are used to identify genomic loci involved in developmental processes. (biomedcentral.com)
  • AIM1, a novel non-lens member of the betagamma-crystallin superfamily, is associated with the control of tumorigenicity in human malignant melanoma. (unige.ch)
  • Beta-crystallins construct aggregates of various sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. (prospecbio.com)
  • Four ethylene receptors genes ( AcERS1b , AcERS1b , AcETR2a , and AcETR2b ) have been cloned, and expression analysis showed that AcERS1b , AcETR2a , and AcETR2b play key roles in pineapple flowering 13 . (nature.com)
  • His Molecular biology study combines topics from a wide range of disciplines, such as In vitro, Transgene, Cataracts, Mutant and Regulation of gene expression. (research.com)
  • The concurrent advance in bioinformatic methods, as well as in high throughput technologies for the analysis of gene expression profiles, allowed deepening the knowledge of genome structure and function. (biomedcentral.com)
  • Given these experimental evidences, the integration of high-throughput transcriptional data with gene structural information and functional characteristics represents a major challenge for bioinformatics and computational biology. (biomedcentral.com)
  • The ClinGen gene curation framework results suggested that variants in different domains of MAF are associated with different diseases. (bvsalud.org)
  • His study in Molecular biology is interdisciplinary in nature, drawing from both Phenotype, Gene, Transgene, Mutation and Retina. (research.com)
  • His Crystallin research is multidisciplinary, relying on both Cytoplasmic inclusion, Glutathione and Molecular mass. (research.com)
  • Eric F. Wawrousek spends much of his time researching Cell biology, Immunology, Crystallin, Molecular biology and Inflammation. (research.com)
  • His study looks at the relationship between Phenotype and fields such as Interferon gamma, as well as how they intersect with chemical problems. (research.com)
  • Recognition of a single-gene disorder as causal for a patient's 'multiple sclerosis-like' phenotype is critically important for accurate direction of patient management, and evokes broader genetic counselling implications for affected families. (medscape.com)
  • In this study, a genetically stable, marker-free recombinant MVA expressing α-crystallin of M. tuberculosis (rMVA. (nature.com)
  • The effect of CTGF silencing on the TGF-β2-modulated expression of these genes was investigated by transfection of CTGF small interfering (si)RNA before TGF-β2 treatment. (arvojournals.org)
  • Using a luciferase indicator driven by the Ccl8 gene promoter, we identified two small compounds that inhibit transcriptional activity of c-Maf. (bvsalud.org)
  • This tadpole is expressing GFP driven by CMV promoter and RFP driven by gamma-crystallin promoter. (wixsite.com)
  • 1991). "Human gamma-crystallin genes. (wikipedia.org)
  • Prior investigation of the refolding of human gammaD-crystallin revealed that the C-terminal domain folds first and nucleates the folding of the N-terminal domain. (protabank.org)
  • We constructed and expressed polypeptide chains corresponding to the isolated N- and C-terminal domains of human gammaD-crystallin, as well as the isolated domains of human gammaS-crystallin. (protabank.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Ras Homolog Gene Family, Member A (RHOA) in samples from tissue homogenates, cell lysates or other biological fluids. (hiv-pharmacogenomics.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Ras Homolog Gene Family, Member A (RHOA) in Tissue homogenates, cell lysates and other biological fluids. (hiv-pharmacogenomics.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Ras Homolog Gene Family, Member A (RHOA) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (hiv-pharmacogenomics.org)
  • The correlation among co-expression and co-localization of genes was also investigated and confirmed in different organisms [ 12 ]. (biomedcentral.com)
  • For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
  • Expression of the murine alpha B-crystallin gene is not restricted to the lens. (research.com)
  • These molecules impede the proliferation of c-Maf-expressing myeloma cells, and repress the expression of c-Maf target genes such as ITGB7 and CCR1. (bvsalud.org)
  • We hypothesized that gene expression patterns attributable to chemotherapy-resistant cells can predict response and cancer prognosis. (gsea-msigdb.org)
  • We interrogated cDNA arrays with 43 000 cDNA clones ( approximately 30 000 unique genes) to study the expression pattern of these cell lines. (gsea-msigdb.org)
  • We divided gene expression profiles into two sets: we compared the expression patterns of the daunorubicin/doxorubicin-resistant cell lines and the mitoxantrone-resistant cell lines independently to the parental cell lines. (gsea-msigdb.org)
  • The patient group characterized by the gene expression profile similar to those of doxorubicin-sensitive cell lines exhibited longer survival (49.7+/-26.1 months, n=21, P=0.034) than the resistant group (32.9+/-18.7 months, n=23). (gsea-msigdb.org)
  • The application of gene expression signatures derived from doxorubicin-resistant and -sensitive cell lines allowed to predict effectively clinical survival after doxorubicin monotherapy. (gsea-msigdb.org)
  • DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
  • His study explores the link between Crystallin and topics such as Knockout mouse that cross with problems in Anatomy. (research.com)
  • Eric F. Wawrousek focuses on Cell biology, Immunology, Inflammation, Innate immune system and Conditional gene knockout. (research.com)
  • As part of one scientific family, Eric F. Wawrousek deals mainly with the area of Conditional gene knockout, narrowing it down to issues related to the Retinal pigment epithelium, and often Cellular homeostasis. (research.com)
  • For identifying predictive genes, the Prediction Analysis for Mircorarrays algorithm was used. (gsea-msigdb.org)
  • His Cell biology research is multidisciplinary, incorporating elements of CD28, Genetically modified mouse, Retinal and Cathepsin D. His Crystallin study combines topics in areas such as Mutant and Notch signaling pathway. (research.com)
  • His work carried out in the field of Retinal brings together such families of science as Crystallin, Notch signaling pathway and Optic nerve. (research.com)
  • Description: A sandwich ELISA kit for detection of Ras Homolog Gene Family, Member A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (hiv-pharmacogenomics.org)
  • Alterations in DNA methylation, lncRNAs and multiple genes may be involved in the regulation of FTLs . (nature.com)
  • Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. (wikipedia.org)