The following product was used in this experiment: ZO-1 Monoclonal Antibody (ZO1-1A12) from Thermo Fisher Scientific, catalog # 33-9100, RRID AB_2533147. (thermofisher.com)
Western blot was performed using Anti-ZO-1 Monoclonal Antibody (ZO1-1A12) (Product # 33-9100) and a 225 kDa band corresponding to ZO-1 was observed across along with uncharacterized band. (thermofisher.com)
Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67, RA22150, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin in green. (neuromics.com)
Specific monoclonal antibodies2
IFM, when coupled with the use of specific monoclonal antibodies, can provide considerable insight into not only the major type of epidermolysis bullosa but also into the structural protein most likely mutated. (medscape.com)
The Ki67 protein was first discovered when researchers attempted to generate cancer cell specific monoclonal antibodies by injecting mice with nuclear preparations from Hodgkin's lymphoma cells. (neuromics.com)
Myosin3
Digenic mutational inheritance of the integrin alpha 7 and the myosin heavy chain 7B genes causes congenital myopathy with left ventricular non-compact cardiomyopathy. (cusabio.com)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Receptor2
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. (cusabio.com)
Acts as Schwann cell receptor for laminin-2. (cusabio.com)
Vitro1
Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). (cusabio.com)
Antigen2
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Skin biopsy using transmission electron microscopy (TEM) and/or immunofluorescent antibody/antigen mapping can be performed but is no longer the preferred method of diagnosis. (nih.gov)
Pathways2
Furthermore, the expression of the genes known to govern insulin expression including Pdx1 and Ngn3 was markedly induced by laminin 411, which suggests that Pdx 1 and Ngn 3 signaling pathways are involved in laminin 411 induced-insulin expression machinery. (biomedcentral.com)
Our results demonstrate that laminin 411 acts as a potent differentiation inducer of IPCs from UC-MSCs via the Pdx1 and Ngn3 signaling pathways. (biomedcentral.com)
Peptides1
Sleep deprivation also caused accumulation of Aß peptides in rest period (13 hr), while laminin levels were not affected. (bvsalud.org)
Polymerase Chain Re1
Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
VERTEBRATES1
The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
Blot2
The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot. (neuromics.com)
Protein4
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. (neuromics.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids. (elisareagents.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Phospholipid Transfer Protein (PLTP) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (elisareagents.com)
Myofibers1
There was no correlation between myofibers with BrdU-positive myonuclei and neonatal MyHC isoform expression. (arvojournals.org)
Integrin1
The absence of either alpha7beta1 integrin or alpha6beta1 integrin impairs the ability of Schwann cells to spread and to bind laminin. (cusabio.com)
Amino2
The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
Although Ki67 is relatively poorly conserved in amino acid sequence, this antibody recognizes both rat and mouse Ki67. (neuromics.com)
Detection4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cytochrome b (COB) in Tissue homogenates, cell lysates and other biological fluids. (antibody-tech.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cytochrome b (COB) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (antibody-tech.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Ras Homolog Gene Family, Member A (RHOA) in Tissue homogenates, cell lysates and other biological fluids. (hiv-pharmacogenomics.org)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Ras Homolog Gene Family, Member A (RHOA) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (hiv-pharmacogenomics.org)
Highlights1
These novel finding highlights a potential clinical application of laminin 411 induced-IPCs in the treatment of T1DM, which calls for further studies. (biomedcentral.com)
Genes1
The identified genes include those that encode keratins 5 and 14 in epidermolysis bullosa simplex, collagen VII in dystrophic epidermolysis bullosa, and laminin 5 in Herlitz junctional epidermolysis bullosa. (medscape.com)
Expression1
Considering that sleep-wake cycle is important for synaptic homeostasis, we aimed to investigate how sleep deprivation affects the expression of PrPC and its ligands, laminin, Aßo, and mGluR1, a multicomplex that can interfere with neuronal plasticity. (bvsalud.org)
Human1
however, little is known about a role of laminin 411 in the regulation of IPC differentiation from human MSCs. (biomedcentral.com)
Avian1
Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses. (lookformedical.com)
Promote1
Among numerous ligands, laminin (LN) is known to promote neurite outgrowth and memory consolidation, while amyloid-beta oligomers (Aßo) trigger synaptic dysfunction. (bvsalud.org)
Heavy1
Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
Alpha1
The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
Structure1
Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. (lookformedical.com)
Function1
JavaScript function together allows to reactions of the chain after the signaling cysteine is trained bound. (erik-mill.de)