• T2CA inhibited the expression of phosphorylated galectin-3 expression in human glioblastoma cell line which in turn inhibits the activity of MMP2 and MMP9 proteins in zymography investigations and inhibits migration of cancer cells in in vitro scratch assay. (arjournals.org)
  • METHODS: We studied the inflammatory response to cuprizone in the SVZ and CC in Gal-3 knockout mice using immunohistochemistry and with the in vitro neurosphere assay. (ox.ac.uk)
  • For in vitro studies, lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC) primary cultures from early-stage patients and commercial cell lines were cultured, using tumorsphere-forming assays and adherent conditions for the control counterparts. (qxmd.com)
  • While different methods exist to evaluate the efficacy of immunotherapy products, more efficient and robust in vitro assays are still required to ensure their safety and consistency. (technologynetworks.com)
  • While many methods have been developed to screen and evaluate the efficacy of immune cell‑mediated killing, there is a need for a more robust in vitro assay to accurately predict the in vivo behavior of therapies. (technologynetworks.com)
  • The Alinity, ARCHITECT, ACCELERATOR and CELL-DYN systems are intended for performing in vitro diagnostic assays on samples of human origin (blood, urine). (corelaboratory.abbott)
  • Galectin-3 is a beta-galactoside-binding mammalian lectin that is one of a 15-member galectin family that can bind several cell surface glycoproteins via its carbohydrate recognition domain (CRD). (lu.se)
  • Background Galectin-9 is a member of the family of lectin proteins and crucially regulates human immune responses, particularly because of its ability to suppress the anticancer activities of T lymphocytes and natural killer cells. (bmj.com)
  • Galectin-3 is a carbohydrate-binding lectin whose expression is associated with inflammatory cells including macrophages, neutrophils, and mast cells. (healthmatters.io)
  • Goose Lectin-Galactose Binding-Soluble three ELISA Equipment from MYBIOSOURCE INC. Product Identify Goose Lectin-Galactose Binding-Soluble three ELISA Equipment Description Assay Sort: Sandwich. (bioinfor.me)
  • TUNEL assay proven significantly less cardiomyocyte apoptosis. (her2signaling.com)
  • It has been suggested in published literature that the beta-galactoside binding protein, galectin-9, acts on T cells to induce apoptosis, but whether this induced cell death is dependent on TIM3 remains an area of active debate and conflicting results. (emerging-researchers.org)
  • We hypothesized that Galectin-9 induced apoptosis of exhausted Jurkat T lymphocytes is dependent on T Cell Immunoglobulin and Mucin Protein 3 (TIM3). (emerging-researchers.org)
  • Galectin-9 protein ability to induce apoptosis was investigated using Jurkat T lymphocytes placed under an untreated negative control, a positive control of staurosporine, and 50, 100, or 200Nm of galectin-9 for 4, 12, and 18 hours. (emerging-researchers.org)
  • I was responsible for treating and splitting all of the Jurkat cells used for the experiments, conducting caspase and tunnel assays to determine levels of apoptosis, determining the puromycin kill curve, performing CRISPR Cas9 gene knockout on Jurkat cells through insertion of puromycin resistance and CRISPR guide RNA. (emerging-researchers.org)
  • Secreted galectin-9 pre-opsonised T cells and, following interaction with other immune checkpoint proteins, their activity was completely attenuated. (bmj.com)
  • As an example, we studied the cooperation of galectin-9 and V-domain Ig-containing suppressor of T cell activation (VISTA) proteins in human cancer cells. (bmj.com)
  • Western blot hybridization studies showed that T2CA downregulates the expression of total gal 3 proteins and extended the survival of AKR/J mice injected with Ehrlich ascites tumor cells in the Kepler-Meier survival curve analysis. (arjournals.org)
  • for 10?a few minutes to split up the pellet (unlysed cells and nuclear small percentage) in the cytoplasm (supernatant) as well as the proteins content from the supernatant was dependant on using the BCA assay (Pierce Thermo Scientific). (techblessing.com)
  • We also noticed that 3 freeze-thaw cycles of myocardial tissues yielded near-maximal proteins release in to the supernatant in the lack of manual manipulation from the tissues (Amount 1A). (techblessing.com)
  • Enzyme-linked immunosorbent assay (ELISA) was employed to measure levels of five serum proteins previously demonstrated to be up-regulated in papillary thyroid cancer (PTC): angiopoietin-1 (Ang-1), cytokeratin 19 (CK-19), tissue inhibitor of metalloproteinase-1 (TIMP-1), chitinase 3 like-1 (YKL-40), and galectin-3 (GAL-3). (biomedcentral.com)
  • The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. (rndsystems.com)
  • This gene encodes a member of the galectin family of carbohydrate binding proteins. (origene.com)
  • MiRNAs bind to 3′ untranslated regions (3′-UTRs) of mRNAs and decrease the translation and/or balance of this mRNA resulting in a decrease in proteins levels. (liveconscience.com)
  • RESULTS: Galectin 3 induced secretion of interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor, CXCL8, and MMP-3 in both synovial and skin fibroblasts. (ox.ac.uk)
  • By contrast, galectin 3-induced secretion of TNFalpha, CCL2, CCL3, and CCL5 was significantly greater in synovial fibroblasts than in skin fibroblasts. (ox.ac.uk)
  • CONCLUSION: Our findings indicate that galectin 3 promotes proinflammatory cytokine secretion by tissue fibroblasts. (ox.ac.uk)
  • Our aims were to elucidate whether T cells can induce galectin-9 secretion in human cancer cells derived from solid malignant tumors and whether this soluble form displays higher systemic immunosuppressive activity compared with the cell surface-based protein. (bmj.com)
  • Results We discovered that T lymphocytes induce galectin-9 secretion in various types of human cancer cells derived from solid malignant tumors. (bmj.com)
  • This was demonstrated to occur via two differential mechanisms: first by translocation of galectin-9 onto the cell surface followed by its proteolytic shedding and second due to autophagy followed by lysosomal secretion. (bmj.com)
  • For both mechanisms a protein carrier/trafficker was required, since galectin-9 lacks a secretion sequence. (bmj.com)
  • We analyzed the pattern of secretion and expression of GAL-3 using reverse transcription-quantitative real-time PCR (RTqPCR), immunoblot, immunofluorescence, flow cytometry and immunoassay analysis. (qxmd.com)
  • At present, guidelines do not recommend using the biomarker assays that are currently available to replace cystoscopy. (medscape.com)
  • However, biomarker assays may provide additional molecular information to guide individualized surveillance and therapy. (medscape.com)
  • Plasma galectin-3 (Gal-3) is associated with organ fibrosis, but whether urinary Gal-3 is a potential biomarker of kidney disease progression has never been explored. (mdpi.com)
  • Levels of galectin-3 in blood may be increased in People with certain forms of advanced cancer and other conditions associated with organ fibrosis. (healthmatters.io)
  • Heterophile antibodies, in particular human-antimouse antibodies in human serum, may cause falsely elevated galectin-3 results. (healthmatters.io)
  • Presence of human antimouse antibodies (HAMA) or rheumatoid factor (RF) may interfere with the galectin-3 assay, which could cause falsely elevated results. (healthmatters.io)
  • Galectin-3 results should be interpreted with caution in patients with a history of therapeutic use of murine monoclonal antibodies (IgG) or their fragments or in those who have known autoimmune disorders. (healthmatters.io)
  • Flow laser technology is used in the assay to detect each microsphere, preventing interference from heteroantigens (antibodies) in the specimen. (creative-proteomics.com)
  • enzyme-linked immunosorbent assay for galectin-3 and soluble ST-2. (unboundmedicine.com)
  • In this investigation, we describe the galectin-3 inhibitory and immune stimulating properties of a novel water-soluble nutraceutical made from Tinospora cordifolia stem. (arjournals.org)
  • Soluble galectin-3 as a microenvironment-relevant immunoregulator with prognostic and predictive value in lung adenocarcinoma. (qxmd.com)
  • Our results using three-dimensional (3D) models of lung tumor cells revealed that soluble GAL-3 (sGAL-3) is highly expressed and secreted. (qxmd.com)
  • METHODS: Matched samples of RA synovial and skin fibroblasts were pretreated with galectin 3 or tumor necrosis factor alpha (TNFalpha), and the levels of a panel of cytokines, chemokines, and matrix metalloproteinases (MMPs) were determined using enzyme-linked immunosorbent assays and multiplex assays. (ox.ac.uk)
  • Previous studies have demonstrated the effects of galectins on immune cells, such as lymphocytes and macrophages. (ox.ac.uk)
  • Different expression pattern of TIM-3 and Galectin-9 molecules by peripheral and peritoneal lymphocytes in women with and without endometriosis. (istanbul.edu.tr)
  • Concentrations of galectin-3 have been used to predict adverse remodeling after a variety of cardiac insults. (healthmatters.io)
  • Concentrations of galectin-3 in the sera of normal controls and cancer patients. (arjournals.org)
  • INTENDED USE AND DESCRIPTION: For use a quantitative control for the determination of human Galectin-3 concentrations in biological fluids. (rndsystems.com)
  • However, whether Galectin-1 regulates receptor tyrosine kinases by sensitizing HCC to sorafenib remains unclear. (aging-us.com)
  • These findings indicate that Galectin-1 regulates sorafenib resistance in HCC cells through AXL and MET signaling. (aging-us.com)
  • The healthy SVZ contains semi-activated microglia and constitutively expresses the pro-inflammatory molecule galectin-3 (Gal-3) suggesting the niche uniquely regulates inflammation. (ox.ac.uk)
  • Galectin-3 protein levels are considered a prognostic tool in the assessment of diagnosed HF. (unboundmedicine.com)
  • Galectin-1 expression was positively correlated with poor prognostic outcomes for HCC patients. (aging-us.com)
  • The Prognostic Value of Plasma Galectin 3 in HFrEF Depends on the Etiology of Heart Failure: a Cohort Study and Animal Experiment. (abbkine.com)
  • The present study aims to analyze the role of galectin-3 (GAL-3) in the lung tumor microenvironment (TME) using tumorspheres as a model and explore its potential role as a predictive and prognostic biomarker in non-small cell lung cancer (NSCLC) patients. (qxmd.com)
  • Next, we evaluated levels of sGAL-3 before surgery in LUAD and LUSC patients, hypothesizing that sGAL-3 could be used as an independent prognostic biomarker for overall survival and relapse-free survival in early-stage LUAD patients. (qxmd.com)
  • Prognostic biomarkers for precision medicine in heart transplant: Is galectin-3 the one? (cdc.gov)
  • Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways. (ox.ac.uk)
  • This study was undertaken to investigate the hypothesis that galectin 3 induces proinflammatory effects in RA by modulating the pattern of cytokine and chemokine production in synovial fibroblasts. (ox.ac.uk)
  • However, galectin 3 induces the production of mononuclear cell-recruiting chemokines uniquely from synovial fibroblasts, but not matched skin fibroblasts, via a PI 3-kinase signaling pathway. (ox.ac.uk)
  • Galectin-3 induces endothelial cell morphogenesis and angiogenesis. (arjournals.org)
  • Collectively, our results underline that the high activity of JNK/c-Jun-ATF2/Galectin-1 mediates cisplatin resistance in liver cancer and provides an optional scheme for dynamic monitoring of molecular activity in vivo . (ijbs.com)
  • Inohara H, Raz A. Functional Evidence That Cell-Surface Galectin-3 mediates homotypic cell-adhesion. (arjournals.org)
  • Galectins play an important and complex role in intracellular pathways and disease mechanisms. (healthmatters.io)
  • Hemolysis has been shown to interfere with the galectin-3 assay due to intracellular release of galectin-3. (healthmatters.io)
  • Galectin-1 is the essential link between the sugar signal and the intracellular response. (illinois.edu)
  • Specific inhibitors were used to dissect signaling pathways, which were confirmed by Western blotting and NF-kappaB activation assay. (ox.ac.uk)
  • As such, galectin-9 and regulatory pathways controlling its production should be considered as key targets for immunotherapy in a large number of cancers. (bmj.com)
  • Galectin-3 is a biomarker that appears to be actively involved in both the inflammatory and some fibrotic pathways. (healthmatters.io)
  • Galectin-3 has recently been proposed as a novel biomarker for cardiovascular disease in adults. (scilifelab.se)
  • Galectin-3 has been linked to obesity and been proposed to be a novel biomarker for cardiovascular disease in adults. (scilifelab.se)
  • However, solid tumor cells at rest are known to secrete either very low amounts of galectin-9 or, in most of the cases, do not secrete it at all. (bmj.com)
  • Liu FT, Rabinovich GA. Galectins as modulators of tumor progression. (arjournals.org)
  • Deletion of galectin-3 in the host attenuates metastasis of murine melanoma by modulating tumor adhesion and NK cell activity. (arjournals.org)
  • Matrices listed below were spiked with certain level of recombinant Galectin 1 (GAL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Galectin 1 (GAL1) in samples. (uscnk.com)
  • The consequences of NMCs on cell proliferation and collagen gene appearance had been mimicked by many recombinant DAMPs including HMGB1 and galectin-3. (techblessing.com)
  • Chemotherapy, including cisplatin (CDDP), remains a potent treatment for patients with late-stage liver cancer [ 2 , 3 ]. (ijbs.com)
  • In 57 patients admitted to the intensive care unit (ICU) with sepsis, serum Gal-3 was examined as a predictor of ICU mortality and development of AKI. (biomedcentral.com)
  • However, no previous study has examined the relationship between serum Gal-3 at the time of admission and the subsequent development of AKI in patients with sepsis. (biomedcentral.com)
  • Galectin-3 results should be interpreted with caution in such patients. (healthmatters.io)
  • Galectin-3 results from patients with diseases associated with hyperglobulinemia, such as multiple myeloma, should be interpreted with caution. (healthmatters.io)
  • MET and AXL were highly expressed in patients with HCC, and AXL expression was positively correlated with Galectin-1 expression. (aging-us.com)
  • Consequently, Galectin-1 is a promising therapeutic target for reducing sorafenib resistance and sorafenib-mediated ferroptosis in patients with HCC. (aging-us.com)
  • Gal-3 and Lp(a) was measured in serum samples from patients and 17 healthy controls by immunometric assays. (unina.it)
  • Results: Gal-3 and Lp(a)/LDL serum levels showed increased levels in patients compared with the control group (p¼0.0001 and p¼0.001 respectively). (unina.it)
  • Galectin-3 (Gal-3) is a protein that can bind to β-galactosides, which plays an important role in different biological processes according to the stages of the disease in patients with endometriosis. (istanbul.edu.tr)
  • In the translational phase, we confirmed that patients with high expression levels of GAL-3 had more TREGS , which suggests that tumors may be recruiting this population through GAL-3. (qxmd.com)
  • Additionally, levels of sGAL-3 at pretreatment and first response assessment from plasma to predict clinical outcomes in advanced LUAD and LUSC patients treated with first-line pembrolizumab were evaluated, further supporting that sGAL-3 has a high efficiency in predicting durable clinical response to pembrolizumab with an area under curve (AUC) of 0.801 (p=0.011). (qxmd.com)
  • Materials and Methods: The serum concentration of Gal-3 and cancer antigen 125 (CA-125) from whole blood were measured using enzyme-linked immuno sorbent assay (ELISA) and an auto-analyzer, respectively. (istanbul.edu.tr)
  • Thompson Exercise and Sports Cardiology volume 3: Exercise Risks, Cardiac Arrhythmias and Unusual Problems in Athletes World Scientific Publishing. (westminster.ac.uk)
  • Galectin-3 levels relate in children to total body fat, abdominal fat, body fat distribution, and cardiac size. (scilifelab.se)
  • The present study demonstrates a relationship between galectin-3 levels and total body fat, abdominal fat, body fat distribution, cardiac size and geometry, and increase in total body fat over 2 years in young children. (scilifelab.se)
  • Fibroblast Proliferation The proliferation of main cardiac fibroblasts and NIH/3T3 fibroblast proliferation was determined by measuring bromodeoxyuridine (5-bromo-2′-deoxyuridine [BrdU]) incorporation as we have explained previously 9 using a commercially available assay (Colorimetric Cell Proliferation ELISA Roche). (techblessing.com)
  • Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. (rndsystems.com)
  • Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. (rndsystems.com)
  • Due to a Cterminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. (liveconscience.com)
  • Historically, the screening and triaging of small molecule glycomimetics that bind to the galectin-3 CRD has been completed in fluorescence polarisation (FP) assays to determine K D values. (lu.se)
  • Galectins bind to certain carbohydrates. (healthmatters.io)
  • Galectin-1 modulates cell-cell and cell-matrix interactions and plays a crucial role in HCC progression. (aging-us.com)
  • Conclusion: Galectin-3 can play critical functions in the development and progression of endometriosis, so, further studies are needed in this area. (istanbul.edu.tr)
  • Moreover, high levels might predict decreased progression-free survival and overall survival to anti-PD-1 therapy, with sGAL-3 being a prognosis-independent biomarker for advanced LUAD. (qxmd.com)
  • Galectin-3 interacts with carbohydrates, such as N-acetyllactosamine, certain cell surface receptors (such as macrophage CD11b/CD18) and extracellular receptors (such as collagen). (healthmatters.io)
  • Using the way human galectin-1 interacts with this complex natural ligand as template, we first defined equivalent positioning for distinct substitutions in the other tested proto-type galectins, e.g. (illinois.edu)
  • Conclusion Our results underline a crucial role of galectin-9 in anticancer immune evasion. (bmj.com)
  • with some assays, BNP levels may be transiently and significantly elevated at the time of administration and must be interpreted with caution. (unboundmedicine.com)
  • Herein, we established a sorafenib-resistant HCC cell line (Huh-7/SR) and determined that Galectin-1 expression was significantly higher in Huh-7/SR cells than in parent cells. (aging-us.com)
  • BrdU+ SVZ cell numbers also decreased in the SVZ after cuprizone, and this effect was significantly greater at 3 weeks in Gal-3 (-/-) mice compared to WT, suggesting Gal-3 normally limits SVZ cell emigration following cuprizone treatment. (ox.ac.uk)
  • NMCs provoked a significant increase in fibroblast proliferation α-clean muscle mass actin activation and collagen 1A1 and 3A1 mRNA manifestation and significantly improved fibroblast motility inside a cell-wounding assay within a Toll-like receptor 4 (TLR4)- and receptor for advanced glycation end products-dependent way. (techblessing.com)
  • Interaction with Galectin-3 (GAL3) was identified, and confirmed by binding assay. (nih.gov)
  • Galectin-3 has been implicated in various diseases, including fibrotic disorders and cancer, and is now being therapeutically targeted by both small and large molecules. (lu.se)
  • These data provide further evidence of the role of synovial fibroblasts in regulating the pattern and persistence of the inflammatory infiltrate in RA and suggest a new and important functional consequence of the observed high expression of galectin 3 in the rheumatoid synovium. (ox.ac.uk)
  • Protein variety discovered a good angiogenic (vascular endothelial growth aspect, endothelin, and epiregulin), anti-apoptotic (Galectin-3, Smad-5, sRFP-1, and also sRFP-4) as well as anti-remodeling elements. (her2signaling.com)
  • Hence galectin-3 levels, once elevated, remain generally constant and do not fluctuate with signs and symptoms of heart failure. (healthmatters.io)
  • Galectin-3 has not been shown to be useful in the acute diagnosis of heart failure, and natriuretic peptides (BNP or NT-proBNP) should be utilized for this purpose. (healthmatters.io)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). (uscnk.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). (uscnk.com)
  • After TMB substrate solution is added, only those wells that contain Galectin 1 (GAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (uscnk.com)
  • Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. (thermofisher.com)
  • It might be exploited therapeutically by producing galectin variants tailored to interfere with galectin activities associated with the malignant phenotype. (illinois.edu)
  • NMC stimulation led to a substantial 3- to 4-flip activation of Akt and Erk whereas pretreatment with Akt (A6730) AST-6 and Erk (U0126) inhibitors reduced NMC-induced fibroblast proliferation dose-dependently. (techblessing.com)
  • Two experimental groups received the Gal-3 inhibitor modified citrus pectin (P-MCP) at 400 mg/kg/day and 1200 mg/kg/day, while the control group received water only ( n = 18 in each group). (biomedcentral.com)
  • invasion assay Matrigel invasion assays had been used to measure the ability from the 9L cells to penetrate the excess Cellular Matrix (ECM) in the existence or lack of miRNA and inhibitor. (liveconscience.com)
  • Surface plasmon resonance (SPR) has not been widely used for compound screening and in this study it was used to compare human and mouse galectin-3 affinity measures between FP and SPR, as well as investigate compound kinetics. (lu.se)
  • The K D estimates for a set of compounds selected from mono- and di-saccharides with affinities across a 550-fold range, correlated well between FP and SPR assay formats for both human and mouse galectin-3. (lu.se)
  • Increases in affinity for compounds binding to human galectin-3 were driven by changes in both k on and k off whilst for mouse galectin-3 this was primarily due to k on . (lu.se)
  • The reduction in affinity observed between human to mouse galectin-3 was also comparable between assay formats. (lu.se)
  • Recent evidence demonstrated that galectin-9 is highly expressed in a wide range of human malignancies including the most aggressive tumors, such as high-grade glioblastomas and pancreatic ductal adenocarcinomas, as well as common malignancies such as breast, lung and colorectal cancers. (bmj.com)
  • The inhibitory effect of T2CA on phopho-galectin-3 expression and the activation of T-cytotoxic and NK cells suggested its protective effects against pathogenic infections and human malignancies via its immune stimulating mechanisms. (arjournals.org)
  • Differential expression of galectin-1 and galectin-3 during first trimester human embryogenesis. (istanbul.edu.tr)
  • T cell immunoglobulin and mucin protein 3 (TIM3) is a cell surface protein expressed on T helper type 1 cells and is proposed to be a regulator of human T cell exhaustion. (emerging-researchers.org)
  • Be the first to review Simple Plex Control for Human Galectin-3 and earn rewards! (rndsystems.com)
  • Have you used Simple Plex Control for Human Galectin-3? (rndsystems.com)
  • Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). (arjournals.org)
  • Next, a monomeric prototype galectin (CG-14) can impair galectin-1-dependent negative growth control by competitively blocking access to the shared ligand without acting as effector. (illinois.edu)
  • The most commonly used method for measuring immune cell‑mediated killing is the release assay, where effector cell‑mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. (technologynetworks.com)
  • The purpose of this investigation was to assess relationships between galectin-3 levels and total body fat, abdominal fat, body fat distribution, aerobic fitness, blood pressure, left ventricular mass, left atrial size, and increase in body fat over a 2-year period in a population-based sample of children. (scilifelab.se)
  • Two of the markers (Gal-3 & TIMP-1) displayed a greater potential difference, which may warrant further investigation. (biomedcentral.com)
  • In addition, it can also provide early kinetic characterisation of small molecule galectin-3 glycomimetics with robust k on and k off values generated in a high throughput manner. (lu.se)
  • Appropriately we standardized the technique for inducing myocardial cell necrosis through the use of 3 freeze-thaw cycles and pooling the supernatants from 5 to 8 hearts that have been eventually aliquoted and kept at ?70°C. The coefficient of SFRS2 variability for the natural activity of the NMC supernatants assessed with regards to fibroblast proliferation (find afterwards) was ≈6.9% (Figure 1B). (techblessing.com)
  • In contrast to solid-phase assays, cell surface presentation of the ganglioside did not support binding of galectin-5, revealing the first level of regulation. (illinois.edu)
  • 4 xCELLigence real-time cell analysis 6 Diverse cancer immunotherapy applications 6 How does the xCELLigence real-time cell analysis assay work? (technologynetworks.com)
  • While the data provided by these assays help piece together an understanding of different facets of immune cell‑mediated killing, it is important to note that the parameters being reported often do not correlate with target cell killing efficacy in vivo. (technologynetworks.com)
  • Applying rigor and reproducibility standards to assay donor-derived cell-free DNA as a non-invasive method for detection of acute rejection and graft injury after heart transplantation. (cdc.gov)
  • This translational study demonstrates the importance of Gal-3 in the pathogenesis of S-AKI, and its potential utility as a therapeutic target. (biomedcentral.com)
  • Galectin-3 levels reflect the presence of specific underlying disease processes and are not affected by the degree of decompensation. (healthmatters.io)
  • Although certain medical and device treatments appear to be effective in people with elevated galectin-3, galectin-3 plasma levels are generally not affected by these treatments. (healthmatters.io)
  • No correlations have been observed between Gal-3 serum levels and Gal-3 levels in atherosclerotic plaques. (unina.it)
  • No differences were found between Gal-3 serum levels among the different plaques types, nor between complicated and uncomplicated plaques. (unina.it)
  • Both Gal-3 and Lp(a)/LDL levels cannot be considered markers of plaque instability. (unina.it)
  • Gal-3 levels in the serum of women with endometriosis are also remarkably increased compared with the control group. (istanbul.edu.tr)
  • 5. Caserta D, Di Benedetto L, Bordi G, D'Ambrosio A, Moscarini M. Levels of Galectin-3 and stimulation expressed gene 2 in the peritoneal fluid of women with endometriosis: A pilot study. (istanbul.edu.tr)
  • More body fat and abdominal fat, more abdominal body fat distribution, more left ventricular mass, and increased left atrial size were all associated with higher levels of galectin-3. (scilifelab.se)
  • Increase in total body fat over 2 years was also associated with higher levels of galectin-3. (scilifelab.se)
  • OBJECTIVE: High expression of galectin 3 at sites of joint destruction in rheumatoid arthritis (RA) suggests that galectin 3 plays a role in RA pathogenesis. (ox.ac.uk)
  • Frozen serum samples were analyzed for galectin-3 by the Proximity Extension Assay technique. (scilifelab.se)
  • There may be a decrease in signal, but overall, the assay results should not be affected. (biolegend.com)
  • It features an assay that is objective and easy to perform (non-invasive and stain-free), providing quantitative kinetic results under physiologically relevant conditions. (technologynetworks.com)
  • The ideal assay should be objective, simple to perform, provide quantitative kinetic results, and mimic physiologically relevant conditions. (technologynetworks.com)
  • Our assays and analyzers provide accurate, reliable results with rapid turnaround time, aiding clinicians in providing better patient care. (corelaboratory.abbott)
  • Gain confidence in results with excellent precision of our STAT High Sensitive Troponin-I assays. (corelaboratory.abbott)
  • 5, 6, 7] All of these assays may yield false-positive and false-negative results. (medscape.com)
  • Based on a review of these and other published studies using gene expression analysis, we concluded that YKL-40, Gal-3, cytokeratin 19 (CK19), TIMP-1 and Ang-1 were consistently found to be upregulated. (biomedcentral.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate. (uscnk.com)
  • This assay has high sensitivity and excellent specificity for detection of Galectin 1 (GAL1). (uscnk.com)
  • Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders. (istanbul.edu.tr)
  • High sensitivity: The detection range can reach 3~5 orders of magnitude, and the lowest detection concentration can reach 0.1 pg/mL. (creative-proteomics.com)
  • 3, 8] In the future, other newer assays based on telomerase and microsatellite analysis may prove to be a better detection method than urinary cytology. (medscape.com)
  • A study by Sokolova et al of 9 genetic markers for detecting urothelial carcinoma showed that polysomy of chromosomes 3, 7, and 17 and deletion of 9p21 were the most sensitive and specific markers, detecting 95% of recurrent urothelial carcinomas. (medscape.com)
  • This study aimed to elucidate the importance of Gal-3 in endometriosis, to reveal its potential power as a non-invasive diagnostic biomarker in disease etiopathogenesis. (istanbul.edu.tr)
  • The MAPKs p38, JNK, and ERK were necessary for IL-6 production, but phosphatidylinositol 3-kinase (PI 3-kinase) was required for selective CCL5 induction. (ox.ac.uk)
  • Malignant transformation of thyroid follicular cells by galectin-3. (arjournals.org)
  • Mechanistically, the highly activated JNK phosphorylated c-Jun and ATF2 formed a heterodimer to upregulate the expression of Galectin-1, leading to promoting cisplatin resistance in liver cancer. (ijbs.com)
  • Fukumori T, Kanayama HO, Raz A: The role of galectin-3 in cancer drug resistance. (arjournals.org)
  • Galectin-1 knockdown reduced sorafenib resistance in Huh-7/SR cells, whereas Galectin-1 overexpression in Huh-7 cells increased sorafenib resistance. (aging-us.com)
  • Galectin-1 overexpression promoted the phosphorylation of AXL receptor tyrosine kinase (AXL) and MET proto-oncogene, receptor tyrosine kinase (MET) signaling, which increased sorafenib resistance. (aging-us.com)
  • 3 The Glycobiology and Bioimaging Laboratory, Research Centre for Infectious Diseases, Centre de recherche CHU de Québec, 2705 boulevard Laurier, Québec, Québec G1V 4G2, Canada. (nih.gov)
  • 3. (1) An application for a licence, or a provisional licence, to establish, operate or maintain a laboratory, or renewal thereof, shall be submitted to the Director. (ontario.ca)
  • 3) The fee for the issuance or renewal of a provisional licence is $631 plus an additional $100 for each test that the licensee is authorized to perform under the licence that is not listed as a service in the schedule of laboratory benefits. (ontario.ca)
  • 3. That the management and operation of the laboratory is at the address set out in the licence for the laboratory. (ontario.ca)
  • View the core laboratory menu to see the complete list of available assays and in development assays, categorized by instrument and segment. (corelaboratory.abbott)
  • CONCLUSIONS: This study reveals a uniquely regulated inflammatory response in the SVZ and shows that Gal-3 participates in remyelination in the cuprizone model. (ox.ac.uk)
  • Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. (liveconscience.com)
  • Instead of developing your own assays, explore our kits and services to quickly quantify viral antigens! (acrobiosystems.com)
  • As predicted from our in silica work, the tested proto-type galectins have affinity for the pentasaccharide of ganglioside GM 1 . (illinois.edu)
  • Cuprizone increased the density of CD45+/Iba1+ microglial cells and also increased Gal-3 expression in the CC. Surprisingly, the number of Gal-3+ and CD45+ cells decreased in the SVZ after cuprizone, suggesting inflammation was selectively reduced therein. (ox.ac.uk)
  • Gal-3 expression was determined by quantitative real-time polymerase chain reaction (qRT-PCR) from peripheral blood. (istanbul.edu.tr)