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  • precursor
  • NB100-64802 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52kDa under reducing conditions in Western blotting. (novusbio.com)
  • proteins
  • Imperfect DNA mirror repeats in the gag gene of HIV-1 (HXB2) identify key functional domains and coincide with protein structural elements in each of the mature proteins. (nih.gov)
  • We employed dual-color super-resolution microscopy visualizing Gag assembly sites and HIV-1 Env proteins in virus-producing and in Env expressing cells. (nih.gov)
  • HIV-1 Gag had no effect on this process.These results establish that the HIV-1 receptor, CD4, and co-receptor, CXCR4 are differentially regulated by ESCRT proteins. (nih.gov)
  • For example, HIV-1 budding is dependent on Gag-mediated recruitment of the cellular ESCRTs-I, -III, AIP1/Alix and Vps4 proteins. (nih.gov)
  • The results showed that 7d virus had significantly lower replication capacity, poorer protease-mediated processing and viral proteins production. (nih.gov)
  • Characterization of the effects of a 7 amino-acid deletion in p6gag to the HIV-1 proteins expression, release and maturation.MT2 cells were infected with wild type (wt) or deleted-type (7d) recombinant viruses. (nih.gov)
  • CTL responses to Gag, Pol and Nef proteins at 3 month and 1 year post infection were detected with Gamma interferon enzyme-linked immunospot (ELISPOT) assay using optimized consensus overlapping peptides, as well as the viral quasispecies sequences from the synchronous plasma. (ox.ac.uk)
  • Gag and Nef proteins were the main targets of CTL responses during the first year of HIV-1 infection, and this was evident from the data after adjusting for the length of amino acids by dividing the amino acids number of the corresponding protein and multiplying by 100. (ox.ac.uk)
  • The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. (abcam.com)
  • plasma membrane
  • B) Model illustrating how HIV Gag mimics the Tsg101-recruiting function of Hrs and redirects Tsg101 and the ESCRT-I complex to the plasma membrane to facilitate viral budding. (nih.gov)
  • Inhibition was associated with diffuse cytoplasmic staining of HIV Gag rather than accumulation at the plasma membrane, suggesting TRIM22 disrupts proper trafficking. (nih.gov)
  • subtype
  • The replicative capacity of nine CRF07_BC and four subtype B isolates were compared and the results showed that the former had significantly lower replicative capacity than the latter although all of them were CCR5- tropic and non-syncytium inducing viruses. (nih.gov)
  • Multi-layered Gag-specific immunodominant responses contribute to improved viral control in the CRF01_AE subtype of HIV-1-infected MSM subjects. (ox.ac.uk)
  • Genetic
  • Genetic polymorphisms within the MHC encoding region have the strongest impact on HIV disease progression of any in the human genome and provide important clues to the mechanisms of HIV immune control. (ox.ac.uk)
  • glycoproteins
  • HIV-1 carries a relatively low number of glycoproteins in its membrane, and the mechanism of Env recruitment and virus incorporation is incompletely understood. (nih.gov)
  • Recent advances in cryoelectron tomography (cryo-ET) have provided a new approach for determining the 3D structures of the intact virus, the HIV capsid, and the envelope glycoproteins located on the viral surface. (tmc.edu)
  • particles
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains.These observations indicate that Hrs normally functions to recruit Tsg101 to the endosomal membrane.HIV-1 Gag apparently mimics this Hrs activity, and thereby usurps Tsg101 and other components of the MVB vesicle fission machinery to facilitate viral budding. (nih.gov)
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains. (nih.gov)
  • RESULTS: Quantitative mass spectrometric lipidome analysis of highly purified HIV-1 particles revealed that the presence of Nef during virus production from T lymphocytes enforced their raft character via a significant reduction of polyunsaturated phosphatidylcholine species and a specific enrichment of sphingomyelin. (uni-regensburg.de)
  • The appearance of these products was unaffected or only moderately affected by substitutions in the MHR of the Gag-Pol precursor, suggesting that the mutant Gag-Pol precursors were efficiently incorporated into viral particles. (asm.org)
  • Further studies showed that TRIM22 inhibited budding of virus-like particles containing Gag only, indicating that Gag was the target of TRIM22. (nih.gov)
  • TRIM22 did not block the release of MLV or EIAV Gag particles. (nih.gov)
  • Fluorescence
  • c , The change in CD63 or clathrin fluorescence is plotted against the corresponding change in Gag-GFP during the appearance of 25 rapidly and slowly emerging puncta, as well as against the corresponding changes at random areas where no puncta appeared. (nih.gov)
  • cells
  • Electron microscopic examination of cells infected with wild-type or 7d virus demonstrated that the 7d virus had poorer and slower viral maturation processes: more viruses attached to the cell membrane and higher proportion of immature virions outside the cells. (nih.gov)
  • The interaction between p6gag and Alix protein was less efficient in cells infected with 7d virus. (nih.gov)
  • A) Western blot analysis of the cell lysates (left panel) and viral lysates (right panel) from cells infected with wt or 7d viruses. (nih.gov)
  • B) The relative expression levels of PR and RT in the viral lysates of cells infected with wt or 7d virus. (nih.gov)
  • We found HLA-B cytoplasmic domains were more resistant to Nef-mediated down-regulation than HLA-A cytoplasmic domains and demonstrated that these differences affect CTL recognition of virus-infected cells in vitro. (ox.ac.uk)
  • However, altered lipid compositions similar to those observed in virions were also detected in detergent-resistant membrane preparations of virus producing cells. (uni-regensburg.de)
  • This novel activity represents a previously unrecognized mechanism by which Nef could manipulate HIV-1 target cells to facilitate virus propagation in vivo. (uni-regensburg.de)
  • Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. (naver.com)
  • Efficacious early antiviral activity of HIV Gag- and Pol-specific HLA-B 2705-restricted CD8+ T cells. (ox.ac.uk)
  • The association between HLA-B 2705 and the immune control of human immunodeficiency virus type 1 (HIV-1) has previously been linked to the targeting of the HLA-B 2705-restricted Gag epitope KRWIILGLNK (KK10) by CD8(+) T cells. (ox.ac.uk)
  • The TRIM22 expressing cells were infected with different amounts of HIV-1/VSVG pseudotyped virus and analyzed by FACS for GFP expression. (nih.gov)
  • Untagged Gag, Gag-mCherry and Gag-GFP were co-expressed and the cells illuminated with a 488 nm laser. (nih.gov)
  • modulates
  • HIV-1 Gag selectively modulates protein sorting at the MVB, interfering with ESCRT-I dependent but not ESCRT-I independent processes. (nih.gov)
  • motif
  • The effect of Gag was dependent on an ESCRT-I interacting motif within the C-terminal p6 region of Gag. (nih.gov)
  • A conserved P(S/T)AP tetrapeptide motif within Gag (the "late domain") binds directly to the NH2-terminal ubiquitin E2 variant (UEV) domain of Tsg101. (nih.gov)
  • accumulation
  • The observed Env accumulation surrounding Gag assemblies, with a lower density on the actual bud, could facilitate viral spread in vivo. (nih.gov)
  • Expression of HIV-1 Gag attenuated downregulation of CXCR4, resulting in accumulation of undegraded receptors within intracellular compartments. (nih.gov)
  • lymphocytes
  • Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization. (naver.com)
  • immune
  • Keeping Env molecules on the nascent virus low may be important for escape from the humoral immune response, while cell-cell contacts mediated by surrounding Env molecules could promote HIV-1 transmission through the virological synapse. (nih.gov)
  • These data support earlier studies suggesting that increased breadth of the Gag-specific CD8(+) T cell response may contribute to improved HIV immune control irrespective of the particular HLA molecules expressed. (ox.ac.uk)
  • diffuse
  • a , FRET coefficients measured at the beginning and the end of the slow appearance of individual puncta (n=30), compared to FRET coefficients at randomly chosen areas containing diffuse Gag (n=30), in static bright puncta (n=30), in cell-free VLPs (n=100) or in rapidly appearing puncta (n=30). (nih.gov)
  • incorporation
  • The role of the MHR in the incorporation of the Gag-Pol precursor was examined by expressing the Gag and Gag-Pol polyproteins individually from separate plasmids. (asm.org)
  • deletion
  • An HIV-1-NL4-3 mutant virus which contains a 7 amino-acid deletion in p6gag (designated as 7d virus) was generated and its live cycle was investigated. (nih.gov)
  • molecules
  • In addition, the effect of a given peptide on a class I molecule within a mixture of human class I molecules can be distinguished by immunoprecipitation with the monomorphic antibody W6/32 and separation by 1-D isoelectric focussing. (ox.ac.uk)
  • Moreover, by manipulating the RNA export element in the viral genome, we show that the export pathway taken is important for the ability of RNA molecules derived from two viruses to interact and be copackaged. (ox.ac.uk)