Patient's oocytesVivoMetaphaseFollicleBipolarMeiosisCheckpointFollicularInfertility problemsReproductive techniquesFertilizationFormationMouseCellDevelopmentHumanGrowthQualityCellsStageJournalMature oocytesOvarian folliclesCumulus cellsMeioticAssembly checkpointEmbryoProphaseAneuploidyInfertilityCompetenceDysfunctionMitochondrialArrestEmbryosCellularSurvivalPotentiallyOvariesEggsFunctionalMicePregnancyBirthApproachesActivityPatientsEvidenceWorkSubstantialOlder techniquesLifeResearch
Patient's oocytes2
- By 1991, the first human pregnancy was recorded using IVM followed by IVF, and in 1994 the first birth using IVM oocytes from polycystic ovarian syndrome patients was recorded highlighting that PCOS patient's oocytes are capable of maturation. (wikipedia.org)
- Furthermore, the patient's oocytes carrying the mutated MAD2L1BP resumed polar body extrusion (PBE) when rescued by microinjection of full-length MAD2L1BP cRNAs. (bvsalud.org)
Vivo4
- In vitro and in vivo imaging and tracking of intestinal organoids from human induced pluripotent stem cells. (lcibio.com)
- This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
- In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
- This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
Metaphase4
- During ovulation, the primary oocyte will resume meiosis in response to signals, arresting in metaphase meiosis II, ready for fertilization. (wikipedia.org)
- biopsied human ovarian tissue and achieved a 10% rate of maturation from unilaminar follicles into metaphase II by a multi-step culture system: 8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes with activin A and follicle-stimulating hormone (FSH). (wikipedia.org)
- We found that oocytes deprotected from their surrounding layer of follicular cells and expressing the epidermal growth factor (EGF) receptor (EGFR) and the Grb7 adaptor undergo accelerated prophase I to metaphase II meiosis progression upon stimulation by EGF. (bvsalud.org)
- In this study, by whole-exome sequencing (WES), we identified homozygous and compound heterozygous MAD2L1BP variants in three families with female patients diagnosed with primary infertility owing to oocyte metaphase I (MI) arrest. (bvsalud.org)
Follicle6
- These then begin meiosis to form the oocyte within the primordial follicle. (wikipedia.org)
- This follicle consists of the oocyte surrounded by flattened pregranulosa cells. (wikipedia.org)
- Primordial follicles containing the primary oocyte, arrested at prophase of meiosis I, develop into primary follicle containing cuboidal granulosa cells. (wikipedia.org)
- The dominant follicle contains the mature oocyte. (wikipedia.org)
- Priming is the process by which the oocytes are primed with follicle-stimulating hormone (FSH) or human chorionic gonadotrophin (hCG) before retrieval. (wikipedia.org)
- These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
Bipolar2
- Once the kinetochores of chromosomes are correctly attached to bipolar spindles and the SAC is satisfied, the MAD2L1BP, best known as p31comet, binds mitosis arrest deficient 2 (MAD2) and recruits the AAA+-ATPase TRIP13 to disassemble the mitotic checkpoint complex (MCC), leading to the cell-cycle progression. (bvsalud.org)
- Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)
Meiosis2
- Xenopus oocytes are encompassed by a layer of follicular cells that contribute to oocyte growth and meiosis in relation to oocyte maturation. (bvsalud.org)
- In summary, our study provides further insight into the role of the follicular cell layer in oocyte meiosis progression. (bvsalud.org)
Checkpoint1
- The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation of chromosomes throughout cell cycles. (bvsalud.org)
Follicular5
- They showed maturation occurs in isolation from normal follicular environment. (wikipedia.org)
- However, the effects of the interaction between follicular cells and the oocyte surface on meiotic processes are unclear. (bvsalud.org)
- Here, we investigated Xenopus follicular cell function using oocyte signaling and heterologous-expressing capabilities. (bvsalud.org)
- In addition, we determined that oocytes surrounded by their follicular cells expressing EGFR-Grb7 exhibit normal meiotic resumption. (bvsalud.org)
- Furthermore, an O-GlcNAcylation increase (by inhibition of O-GlcNAcase), the glycosidase that removes O-GlcNAc moieties, or decrease (by inhibition of OGT) amplifies oocyte spindle defects when follicular cells are absent highlighting a control of the meiotic spindle by the OGT-O-GlcNAcase duo. (bvsalud.org)
Infertility problems2
- It can be offered to women with infertility problems, combined with In Vitro Fertilization (IVF), offering women pregnancy without ovarian stimulation. (wikipedia.org)
- Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes and preimplantation embryos, and the effect of these changes on the future of offspring is unknown, and has caused concerns. (bvsalud.org)
Reproductive techniques1
- This stress correlates with declining egg quality, impacting assisted reproductive techniques' success. (novoslabs.com)
Fertilization1
- In 1935, Pincus & Enzmann did the first experiment on immature rabbit oocyte, showing in vitro spontaneous maturation and fertilization. (wikipedia.org)
Formation4
- Distinct roles of Cep192 and Cep152 in acentriolar MTOCs and spindle formation during mouse oocyte maturation. (lcibio.com)
- This unusual maturation unravels atypical spindle formation but is rescued by inhibiting integrin ß1 or Grb7 binding to the EGFR. (bvsalud.org)
- These oocytes are protected from abnormal meiotic spindle formation through the recruitment of O-GlcNAcylated Grb7, and OGT (O-GlcNAc transferase), the enzyme responsible for O-GlcNAcylation processes, in the integrin ß1-EGFR complex. (bvsalud.org)
- An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
Mouse1
- cRNA microinjection of full-length or truncated MAD2L1BP uncovered their discordant roles in driving the extrusion of polar body 1 (PB1) in mouse oocytes. (bvsalud.org)
Cell2
- Cell contraction was measured using defocusing microscopy (DM), a technique developed by Agero and colleagues (1, 2). (lcibio.com)
- When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
Development2
- The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. (bvsalud.org)
- Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)
Human2
- Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. (bvsalud.org)
- Together, our studies identified and characterized novel biallelic variants in MAD2L1BP responsible for human oocyte maturation arrest at MI, and thus prompted new therapeutic avenues for curing female primary infertility. (bvsalud.org)
Growth1
- The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth factors and steroids. (wikipedia.org)
Quality1
- This leads to improved maturation and quality of the oocytes. (wikipedia.org)
Cells2
- Oocytes are classified depending on their condition, such as number of cumulus cells. (wikipedia.org)
- MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)
Stage1
- After cRNA injection, oocytes were arrested at the GV stage for 3-4 h in the presence of 2.5 mM milrinone, transferred to freshM16 medium, and cultured for 14 h. (lcibio.com)
Journal1
- The Journal of clinical investigation, 2018. (lcibio.com)
Mature oocytes3
- Of these primordial follicles, only around 400 mature oocytes are released and could be potentially fertilised, with the rest undergoing atresia. (wikipedia.org)
- If many mature oocytes are retrieved, there is an opportunity to carry out several attempts at embryo transfer from a single cycle. (medscape.com)
- Social egg freezing refers to the cryopreservation of mature oocytes on an elective basis for the purpose of delayed childbearing. (bcmj.org)
Ovarian follicles1
- In vitro maturation (IVM) is the technique of letting the contents of ovarian follicles and the oocytes inside mature in vitro. (wikipedia.org)
Cumulus cells2
- Oocytes are classified depending on their condition, such as number of cumulus cells. (wikipedia.org)
- Cryopreservation difficulties include the different optimal times of equilibration for the oocyte and its smaller cumulus cells. (medscape.com)
Meiotic1
- Furthermore, they have to undergo incubation for 24-48 h in culture for meiotic maturation. (medscape.com)
Assembly checkpoint3
- Homer HA, McDougall A, Levasseur M, Murdoch AP, Herbert M. [abstract] Spindle assembly checkpoint dysfunction: a molecular correlate for human aneuploidy . (ncl.ac.uk)
- Homer HA, McDougall A, Levasseur M, Herbert M. Restaging the spindle assembly checkpoint in female mammalian meiosis I . Cell Cycle 2005, 4 (5), 650-653. (ncl.ac.uk)
- The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation of chromosomes throughout cell cycles. (bvsalud.org)
Embryo5
- Although mitochondria are maternally transmitted to the offspring through the oocyte, there has been limited understanding of the role of mitochondria in development and maturation of oocyte, and subsequently, in the development of preimplantation embryo. (nih.gov)
- The quality of mitochondria present at different stages of oocyte development from the primordial germ cell stage to the fertilizable state in the mature follicle, and in the preimplantation embryo have never been clearly defined. (nih.gov)
- Thus, this FOA calls for novel approaches for assessment of mitochondrial health in the oocyte and preimplantation embryo. (nih.gov)
- Our proof-of-concept experiments show that replacement of the entire cytoplasm of oocytes from a sensitive mouse strain overcomes massive embryo developmental arrest characteristic of non-manipulated oocytes. (elifesciences.org)
- 1 ] Initial attempts utilized a technique called slow-freezing that yielded eggs with poor survival and pregnancy rates of approximately 12% per embryo transferred. (bcmj.org)
Prophase1
- Primordial follicles containing the primary oocyte, arrested at prophase of meiosis I, develop into primary follicle containing cuboidal granulosa cells. (wikipedia.org)
Aneuploidy4
- Cooling and exposure to cryoprotecting agents (CPAs) affect the cytoskeleton and may aggravate the already high incidence of aneuploidy in human oocytes. (medscape.com)
- Homer HA, McDougall A, Levasseur M, Yallop K, Murdoch AP, Herbert M. Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes . (ncl.ac.uk)
- Homer HA, McDougall A, Levasseur M, Murdoch AP, Herbert M. RNA interference in meiosis I human oocytes: Towards an understanding of human aneuploidy . (ncl.ac.uk)
- The goal of this study was to use a physiologic aging mouse model, in which egg aneuploidy levels increase, to determine whether there are age-dependent differences in morphokinetic parameters of oocyte maturation. (bvsalud.org)
Infertility5
- This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies. (elifesciences.org)
- Using this technique, it is possible to generate human egg cells for IVF that have the genetic material from the intended mother without the defects in the cytoplasm that may be responsible for infertility. (elifesciences.org)
- These findings open up the possibility of developing new treatments for infertility caused by problems with egg cells, so experiments involving human egg cells are now being performed to evaluate the safety and effectiveness of the technique. (elifesciences.org)
- Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. (bvsalud.org)
- Together, our studies identified and characterized novel biallelic variants in MAD2L1BP responsible for human oocyte maturation arrest at MI, and thus prompted new therapeutic avenues for curing female primary infertility. (bvsalud.org)
Competence1
- Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. (elifesciences.org)
Dysfunction2
- The overarching goal is to gain fundamental insight into the role of mitochondria and long-term consequences of their dysfunction in the oocyte, and to develop therapeutic or alternative approaches to treat mitochondrial dysfunction for improving oocyte quality and competency, and health of the resultant offspring. (nih.gov)
- This funding opportunity announcement encourages innovative and high-risk/impact studies that may lead to breakthrough in mitochondrial function, or to the development of novel techniques, agents, methodologies, models, or applications that could have a major impact in understanding and treatment of mitochondrial dysfunction. (nih.gov)
Mitochondrial3
- The purpose of this Funding Opportunity Announcement (FOA) is to encourage applications from the scientific community to support outstanding research in the area of oocyte mitochondrial function in relation to fertility, aging, and mitochondrial disease transmission to offspring. (nih.gov)
- Yet, how healthy oocytes balance essential mitochondrial activity with the production of ROS is unknown. (bvsalud.org)
- Here we show that oocytes evade ROS by remodelling the mitochondrial electron transport chain through elimination of complex I. Combining live-cell imaging and proteomics in human and Xenopus oocytes, we find that early oocytes exhibit greatly reduced levels of complex I. This is accompanied by a highly active mitochondrial unfolded protein response, which is indicative of an imbalanced electron transport chain. (bvsalud.org)
Arrest1
- Once the kinetochores of chromosomes are correctly attached to bipolar spindles and the SAC is satisfied, the MAD2L1BP, best known as p31comet, binds mitosis arrest deficient 2 (MAD2) and recruits the AAA+-ATPase TRIP13 to disassemble the mitotic checkpoint complex (MCC), leading to the cell-cycle progression. (bvsalud.org)
Embryos2
- Oocyte banking is more problematic than cryopreservation of sperm or embryos. (medscape.com)
- The developmental potential of early embryos is mainly dictated by the quality of the oocyte. (elifesciences.org)
Cellular1
- Although poor oocyte quality accounts for most female fertility problems, little is known about how oocytes maintain cellular fitness, or why their quality eventually declines with age2. (bvsalud.org)
Survival1
- The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth factors and steroids. (wikipedia.org)
Potentially1
- Further disadvantages of this method are that cancer patients may not have more than one opportunity for oocyte harvesting before undergoing potentially sterilizing treatment, since a cycle of controlled stimulation requires several weeks and there is normally a delay of a few months before treatment cycles. (medscape.com)
Ovaries2
- Through in vitro maturation, folliculogenesis and latter parts of oogenesis are being mimicked outside of the ovaries- trying to recreate the conditions for these processes. (wikipedia.org)
- Oocytes can, thus, be recovered from unstimulated ovaries, as well as from children, and egg harvesting is less expensive and risky and can be repeated frequently. (medscape.com)
Eggs2
- 3 ] So even though the older techniques meant that 100 eggs might be needed to achieve one live birth, low success rates were acceptable given the bleak alternative of sterility for cancer patients. (bcmj.org)
- All morphokinetic parameters of oocyte maturation were similar between euploid and aneuploid eggs irrespective of age. (bvsalud.org)
Functional1
- Functional investigation indicated that the interaction is required for lipopolysaccharide (LPS)- and ATP-induced exosome release. (nih.gov)
Mice2
- applied MST to infertile female mice and found that the technique could permanently correct deficiencies in the cytoplasms of poor quality egg cells, allowing the mice to give birth to healthy offspring. (elifesciences.org)
- RESULTS: Oocytes from reproductively old mice were smaller than young counterparts in terms of GV area (446.42 ± 4.15 vs. 416.79 ± 5.24 µm2, p (bvsalud.org)
Pregnancy2
- 10 oocytes means a very low chance of pregnancy). (medscape.com)
- Pregnancy rates were a third to a quarter of the success rates observed with unfrozen oocytes. (medscape.com)
Birth2
- who found that the live-birth rate per injected oocyte was approximately 2% for the most commonly used slow-cooling technique. (medscape.com)
- Oocytes form before birth and remain viable for several decades before fertilization1. (bvsalud.org)
Approaches1
- A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Activity1
- For these investigations, we used PLX4720, an analog of vemurafenib, with virtually indistinguishable activity against BRAF, compared with other BRAF inhibitors, such as vemurafenib or dabrafenib. (thetechnoant.info)
Patients1
- Disease severity correlated with CD71 expression in cells from male and female patients with SLE, and blocking CD71 in vitro enhanced interleukin 10 secretion. (nih.gov)
Evidence1
- 3,5 ] More recently, following substantial advancements in cryopreservation technique and evidence from randomized controlled trials, egg freezing has received mainstream attention. (bcmj.org)
Work1
Substantial1
- Over the past few years, substantial advancements have been made in the egg freezing technique. (bcmj.org)
Older techniques1
- Conclusions Congenital heart disease loss of life prices reduce because gestational grow older techniques Forty five days. (pdgfreceptor.com)
Life1
- Then a prototypical answer to get a full lifestyle paradigm, the two-phase approach will be described as the combination in the strain-life technique and also fracture progress strategies. (pdgfreceptor.com)
Research1
- Existing research is based on snapshot analyses, preventing temporal investigations of network changes. (cambridge.org)