• High levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. (hindawi.com)
  • The aim of this study was to access the potential of Trichoderma harzianum to produce fungal biomass protein from rice polishings. (hindawi.com)
  • The results presented in this study have practical implications in that the fungus T. harzianum could be used successfully to produce fungal biomass protein using rice polishings. (hindawi.com)
  • Biotechnological treatments to food processing wastes which are found in large quantities can produce useful end products including microbial biomass protein (MBP) while the wastes are also purified during the process. (hindawi.com)
  • In this work, ethanol and fungal biomass (animal feed) were produced from acid-pretreated wheat straw slurry under an innovative simultaneous saccharification, fermentation, and filtration (SSFF) strategy. (biofueljournal.com)
  • More specifically, it allows in-situ separation of insoluble solids leading to the production of pure fungal biomass as a value-added product. (biofueljournal.com)
  • The problem is further enhanced when the biomass to be analyzed for protein studies is too little due to the extreme experimental parameters under consideration such as increased or lowered pH, temperature, hydrostatic pressure, nutrients, etc. (springer.com)
  • The method described here is capable of obtaining proteins from minuscule quantities of biomass (~5 mg lyophilized biomass). (springer.com)
  • The protocol described here provides for a simple and quick method for extraction of high-quality proteins from very less biomass that could be extended to other tough to lyse biological material also. (springer.com)
  • The sequence identity between different fungal alpha subunits is relatively low and is equivalent to the level of similarity observed between mammalian alpha subtypes. (ebi.ac.uk)
  • The second and third authors have validated the method by evaluating it for extraction from different fungal cultures. (springer.com)
  • PRO11 shows significant homology to several vertebrate WD40 proteins, such as striatin and zinedin, which seem to be involved in Ca 2+ -dependent signaling in cells of the central nervous system and are supposed to function as scaffolding proteins linking signaling and eukaryotic endocytosis. (asm.org)
  • The NADPH oxidases (Nox) are a widely distributed family of eukaryotic proteins that transfer electrons across biological membranes to catalyze the reduction of molecular oxygen to superoxide ( 1 - 3 ). (pnas.org)
  • G proteins and their receptors (GPCRs) form one of the most prevalent signalling systems in mammalian cells, regulating systems as diverse as sensory perception, cell growth and hormonal regulation [ PMID: 15294442 ]. (ebi.ac.uk)
  • We use a ligation independent cloning to clone target genes into expression vectors for E. coli and P. pastoris and a small scale test expression to identify constructs producing soluble protein. (aau.dk)
  • Cloning of a mouse cDNA encoding striatin allowed functional substitution of the wild-type protein with restoration of fertility in mutant pro11. (asm.org)
  • Cpp1 phosphatase mediated signaling crosstalk between Hog1 and Cek1 mitogen-activated protein kinases is involved in the phenotypic transition in Candida albicans. (semanticscholar.org)
  • There are three tiers of protein kinases within each SAPK module, comprising of a MAPKKK which phosphorylates and activates a MAPKK, which in turn phosphorylates and activates the terminal SAPK. (frontiersin.org)
  • DMI3 , a Medicago truncatula gene that acts immediately downstream of calcium spiking in this signaling pathway and is required for both nodulation and mycorrhizal infection, has high sequence similarity to genes encoding calcium and calmodulin-dependent protein kinases (CCaMKs). (sciencemag.org)
  • The plants exhibit increased resistance to fungal toxins that target ribosomal L3 protein. (ideaconnection.com)
  • Also disclosed are transgenic plants that co-produce L3 mutant and an RIP protein, and exhibit increased resistance to various fungal toxins and viruses, while reducing toxicity normally associated with production of the RIP. (ideaconnection.com)
  • Published data indicate that ciliate and trichomonad hydrogenosomes share common ancestry with mitochondria, but the evolutionary origins of fungal hydrogenosomes have been controversial. (ucl.ac.uk)
  • As the complicated nature of secretory pathways was revealed - namely that there are many non-classical pathways of secretion and there are many non-secreted proteins that are a part of the classical secretory pathway - a more in-depth definition of the secretome became necessary. (wikipedia.org)
  • Lysine acetylation is a key posttranslational modification that regulates diverse proteins involved in a range of biological processes. (pnas.org)
  • Living organisms have evolved protein phosphorylation, a rapid and versatile mechanism that drives signaling and regulates protein function. (sciencemag.org)
  • Guanine nucleotide binding proteins (G proteins) are membrane-associated, heterotrimeric proteins composed of three subunits: alpha ( IPR001019 ), beta ( IPR001632 ) and gamma ( IPR001770 ) [ PMID: 14762218 ]. (ebi.ac.uk)
  • The length of the G protein signal is controlled by the duration of the GTP-bound alpha subunit, which can be regulated by RGS (regulator of G protein signalling) proteins or by covalent modifications [ PMID: 11313912 ]. (ebi.ac.uk)
  • The specific combination of subunits in heterotrimeric G proteins affects not only which receptor it can bind to, but also which downstream target is affected, providing the means to target specific physiological processes in response to specific external stimuli [ PMID: 9278091 , PMID: 11882385 ]. (ebi.ac.uk)
  • Regulation of G proteins by covalent modification. (ebi.ac.uk)
  • Collectively, these results provide a resource for further mechanistic studies examining the regulation of protein function by reversible acetylation and offer insight into the complex immune response triggered by virulent C. carbonum . (pnas.org)
  • We found by yeast two-hybrid and coimmunoprecipitation assays that E. festucae NoxR interacts with homologs of the yeast polarity proteins, Bem1 and Cdc24, and that the Phox and Bem1 (PB1) protein domains found in these proteins are essential for these interactions. (pnas.org)
  • The changes in fungal growth in planta resulted in a defective symbiotic interaction phenotype. (pnas.org)
  • Comparative analysis of fungal and oomycete CYPomes revealed cases of both extreme expansion and contraction. (biomedcentral.com)
  • In Saccharomyces cerevisiae, two GTP-binding alpha subunits of the heterotrimeric G protein have been identified, Gpa1 and Gpa2. (ebi.ac.uk)
  • Methylammonium and ammonium (MEP) permeases of Saccharomyces cerevisiae belong to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH 4 + + NH 3 ). (asm.org)
  • Methylammonium and ammonium permeases MEP1, MEP2, and MEP3 of Saccharomyces cerevisiae ( 35 ) and the ammonium and methylammonium transport B (AmtB) protein of enteric bacteria ( 64 ) are members of a unique family of cytoplasmic membrane transporters that are specific for ammonium ( 48 ). (asm.org)
  • Here we show that the sterile mutant pro11 carries a defect in the pro11 gene encoding a multimodular WD40 repeat protein. (asm.org)
  • Complementation analysis indicates that the wild-type gene or C-terminally truncated versions of the wild-type protein are able to restore the fertile phenotype in mutant pro11. (asm.org)
  • Although much of the extraordinary diversity of the pan-fungal CYPome can be attributed to gene duplication and adaptive divergence, our analysis also suggests a few potential horizontal gene transfer events. (biomedcentral.com)
  • The project aims to understand the structure and function of fungal membrane transport proteins identified as potential new drug targets, using a combination of state-of-the-art structural (X-ray crystallography, cryo-EM) and biochemical/biophysical techniques. (structuralbiology.eu)
  • Experience in protein X-ray crystallography and/or cryo-EM is an advantage. (structuralbiology.eu)
  • We acknowledge fruitful cooperations with the following colleagues: K. Piontek and D. Plattner (University of Freiburg, Germany) in the field of protein crystallography, X. Wang and J. T. Groves (Princeton University, USA) regarding stopped-flow techniques, and J. Atzrodt and W. Holla (Sanofi Frankfurt, Germany) in the field of drug metabolites. (springer.com)
  • Since transmembrane proteins are also processed in the ER, but not secreted, software like the TMHMM server is used to predict transmembrane domains and therefore eliminate false positives. (wikipedia.org)
  • NoxR, a fungal homolog of the phagocytic p67 phox Nox regulator, has been shown to regulate both NoxA and NoxB. (pnas.org)
  • We postulate that MEP proteins facilitate diffusion of NH 3 across the cytoplasmic membrane and speculate that human Rhesus proteins, which lie in the same domain family as MEP proteins, facilitate diffusion of CO 2 . (asm.org)
  • Considering various modes of intra-cellular protein movement and the requirements for such transport, we establish a list of features that would have direct im pact on the recognition of the proteins by the transport ma chinery of the cell. (actapress.com)
  • These proteins interact with each other in vivo at these same cellular sites as shown by bimolecular fluorescent complementation assays. (pnas.org)
  • Fungal NADPH oxidases are also required for cellular growth and differentiation processes associated with plant host infection and colonization. (pnas.org)
  • Bovine serum from cell culture media and cellular debris can contaminate the collection of secreted proteins used for analysis. (wikipedia.org)
  • Expressed soluble proteins are then produced in larger quantities, purified and assayed for new enzymatic activities. (aau.dk)
  • Many secretomic studies are conducted in vitro with cell culture methods, but it is unclear whether the same proteins are secreted in vivo. (wikipedia.org)
  • Lakshman DK, Natarajan SS, Lakshman S, Garrett WM, Dhar AK (2008) Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani . (springer.com)
  • Proteomic approaches are necessary to validate any predicted secreted proteins. (wikipedia.org)