• Both are efficient up to acyl-ACPs with a 14 carbon chain, at which point synthase II is the more efficient choice for further carbon additions. (wikipedia.org)
  • Beta-ketoacyl synthase contains two protein domains. (wikipedia.org)
  • The main function of beta-ketoacyl-ACP synthase is to produce fatty acids of various lengths for use by the organism. (wikipedia.org)
  • Functional characterization of Cpr38, which has sequence similarity to the gene products encoded by pabA and pabB from E. coli , revealed that it functions as a 4-amino-4-deoxychorismate (ADC) synthase catalyzing a two-step reaction involving amidohydrolysis of L-Gln with ammonia channeled and incorporated into chorismic acid to generate ADC. (uky.edu)
  • Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
  • Fatty acid synthase is a multifunctional protein. (thermofisher.com)
  • β-Ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. (rcsb.org)
  • Here we report the findings of structural, enzymatic, and binding studies of the FabG protein found in the causative agent of cholera, Vibrio cholerae (vcFabG). (rcsb.org)
  • In this scenario, hydride is predicted to be added to the re face to generate the S configuration resulting in the same stereochemical outcome as other 3-oxoacyl-ACP reductase (FabG) from bacterial type II fatty acid synthases.These findings are critical advancement for interrogating the biosynthesis of the unusual chemical components of the family of antibiotics of capuramycin. (uky.edu)
  • Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
  • It typically uses malonyl-CoA as a carbon source to elongate ACP-bound acyl species, resulting in the formation of ACP-bound β-ketoacyl species such as acetoacetyl-ACP. (wikipedia.org)
  • Beta-ketoacyl-ACP synthases I and II only catalyze acyl-ACP reactions with malonyl ACP. (wikipedia.org)
  • Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. (thermofisher.com)
  • Protein of unknown function DUF4336 [Interproscan]. (ntu.edu.sg)
  • Protein of unknown function (DUF520) [Interproscan]. (ntu.edu.sg)
  • DMSO reductase anchor subunit (DmsC) [Interproscan]. (ntu.edu.sg)
  • Nitrate reductase delta subunit [Interproscan]. (ntu.edu.sg)
  • Glyceraldehyde 3-phosphate dehydrogenase [Interproscan]. (ntu.edu.sg)
  • The exact mechanism of cell death from GO remains uncertain although a slight increase in lactate dehydrogenase (LDH) from cells, generation of reactive oxygen species, and weak activation of a caspase-3-mediated apoptosis pathway have all been reported. (ikk-signal.com)
  • protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
  • 3-Ketoacyl-ACP reductase [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
  • malonic semialdehyde reductase [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74850","yeaD","Escherichia coli","D-hexose-6-phosphate epimerase-like protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • In the case of a metabolic reaction network, gene or even protein expression data may not best capture the interactions between the network's components, as mRNA levels or enzyme concentrations do not necessarily correlate with reaction rate or metabolite turnover. (biomedcentral.com)
  • Type I FAS catalyzes all the reactions necessary to create palmitic acid, which is a necessary function in animals for metabolic processes, one of which includes the formation of sphingosines. (wikipedia.org)
  • Following condensation, Cpr33 acts as 3-oxoacyl-ACP reductase that catalyzes reduction to the β-hydroxythioester intermediate. (uky.edu)
  • Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
  • In animals, fungi, and lower eukaryotes, Beta-ketoacyl-ACP synthases make up one of the catalytic domains of larger multifunctional proteins (Type I), whereas in most prokaryotes as well as in plastids and mitochondria, Beta-ketoacyl-ACP synthases are separate protein chains that usually form dimers (Type II). (wikipedia.org)
  • In all DD-CPase PBPs, the lysine of the SXXK tetrad acts as a proton acceptor for a nucleophilic attack by serine that facilitates the formation of an acyl-enzyme intermediate IKBKE (Nicholas et al. (phosphorylaseinhibitors.com)
  • It is worth mentioning that during acyl-enzyme complex formation, the terminal d-Ala is removed from the pentapeptide. (phosphorylaseinhibitors.com)
  • GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
  • The encoded protein has been associated with metronidazole resistance, at least for some strains AZD1480 concentration [34, 35], including because it is suspected to Luminespib clinical trial donate electrons to a nitroreductase (the product of the frxA gene) that is required to activate the drug. (inhibitorlibraries.com)
  • Recently, Tang and coworkers used gene expression data to construct time-course protein interaction networks, and found that functional modules detected in the time-course networks more closely matched known regulatory complexes than those detected in the static networks[ 11 ]. (biomedcentral.com)
  • 1016/​S0006-3495(02)75565-3 PubMedCrossRef Georgakopoulou S, Cogdell RJ, van Grondelle R, van Amerongen H (2003) Linear-dichroism GDC-0941 in vitro measurements on the LH2 antenna complex of Rhodopseudomonas acidophila strain 10050 show that the transition dipole moment of the carotenoid rhodopin glucoside is not collinear with the long molecular axis. (tgf-betasignal.com)
  • In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. (thermofisher.com)
  • Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
  • Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)
  • We focus specifically on two diseases: very long-chain acyl-CoA dehydrogenase deficiency (VLCADD) and malonyl-CoA synthetase deficiency (acyl-CoA synthetase family member 3 (ACSF3)) deficiency, which are both characterized by alterations in metabolic flexibility. (nih.gov)
  • 7/29/2013) TOTAL 2014 NEW DESCRIPTORS = 304 MH - 3-Hydroxyacyl-CoA Dehydrogenase UI - D063988 MN - D8.811.682.47.820.150.207 MS - An NAD-dependent 3-hydroxyacyl CoA dehydrogenase that has broad specificity with regards to the acyl chain length of the substrate. (nih.gov)
  • use 3-HYDROXYACYL COA DEHYDROGENASES 1981-2013 MH - 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase UI - D064431 MN - D8.811.682.47.820.196 MS - A 3-oxoacyl reductase that has specificity for ACYL CARRIER PROTEIN-derived FATTY ACIDS. (nih.gov)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • The main function of beta-ketoacyl-ACP synthase is to produce fatty acids of various lengths for use by the organism. (wikipedia.org)
  • and yeast mitochondrial protein CEM1. (wikipedia.org)
  • Mitochondrial substrate/solute carrier [Interproscan]. (ntu.edu.sg)
  • A knowledge graph of biological entities such as genes, gene functions, diseases, phenotypes and chemicals. (edu.sa)
  • Cache domain, Methyl-accepting chemotaxis protein (MCP) signalling domain [Interproscan]. (ntu.edu.sg)
  • Thus, 3-oxohexacosyl-CoA is considered to be a fatty ester lipid molecule. (ymdb.ca)
  • creates a toxic condition, which directly alters the stability of various lipids, proteins, enzymes of critical metabolic pathways, replication, transcription, and translation machinery, which makes it difficult for the micro organisms to survive [ 70 , 73 ]. (sciendo.com)
  • Beta-ketoacyl-ACP synthases I and II only catalyze acyl-ACP reactions with malonyl ACP. (wikipedia.org)
  • Type I FAS catalyzes all the reactions necessary to create palmitic acid, which is a necessary function in animals for metabolic processes, one of which includes the formation of sphingosines. (wikipedia.org)
  • Finally, we also tested the deletion of two potential medium-chain acyl-CoA depleting reactions catalyzed by the thioesterase Tes1 and the medium-chain fatty acyl CoA synthase Faa2. (biomedcentral.com)
  • Effective immobilization was achieved with 2% (w/v) sodium alginate and 3% (w/v) calcium chloride. (bvsalud.org)
  • It typically uses malonyl-CoA as a carbon source to elongate ACP-bound acyl species, resulting in the formation of ACP-bound β-ketoacyl species such as acetoacetyl-ACP. (wikipedia.org)
  • Both are efficient up to acyl-ACPs with a 14 carbon chain, at which point synthase II is the more efficient choice for further carbon additions. (wikipedia.org)
  • part of family of 3'-to5' exonucleases. (lbl.gov)
  • The rare sugar d-allulose is a C-3 epimer of d-fructose and is known to have several health benefits such as anti-obesity and anti-diabetic effects through the alteration of enzymatic and genetic expressions in each organ. (bvsalud.org)
  • Figure 3 Deletion of hfq in H. influenzae strain 86-028NP in the chinchilla model of otitis media. (kspinhibitors.com)
  • is also generated during the nitrite respiration in several bacteria, including E. coli , by the activity of nitrite reductase [ 21 ]. (sciendo.com)