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  • normalized cDNA
  • To achieve a global view of the genes differentially expressed in MSCs, myotube formed-cells (MFCs) and adipocyte-like cells (ALCs), we performed large-scale EST sequencing of normalized cDNA libraries developed from bovine MSCs. (nih.gov)
  • renal
  • Another 2011 study found that the p53 homozygous (Pro/Pro) genotype was associated with a significantly increased risk for renal cell carcinoma. (wikipedia.org)
  • vitro
  • Moreover, hPSC-derived motor neurons were able to form neuromuscular junctions with human myotubes in vitro and induced acetylcholine receptor (AChR) clustering, as detected by Alexa 555-conjugated α-Bungarotoxin (α-BTX), suggesting that these hPSC-derived motor neurons formed functional contacts with skeletal muscles. (biomedcentral.com)
  • For about thirty years stem cells have provided a vast field of research in adult tissue, 1 in embryonic tissue and in in vitro cultures of embryonic stem cells of experimental animals. (lifeissues.net)
  • adult stem
  • These problems underscore the need to search for new sources of adult stem cells to generate cardiomyocytes against the failing myocardium. (hindawi.com)
  • In adults, telomerase is highly expressed only in cells that need to divide regularly especially in male sperm cells[citation needed] but also in epidermal cells, in activated T cell and B cell lymphocytes, as well as in certain adult stem cells, but in the great majority of cases somatic cells do not express telomerase. (wikipedia.org)
  • postnatal
  • Extensive alternative splicing transitions during postnatal skeletal muscle development are required for calcium handling functions. (abcam.com)
  • Mouse periodontal ligaments demonstrated tenomodulin protein expression at 3 and 4 weeks postnatal, a time period corresponding to molar eruptive and post-eruptive phases when the teeth become functional. (wikipedia.org)
  • kidney
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • morphology
  • DDAH2 was up-regulated during myognesis and knockdown of DDAH2 by siRNA significantly decreased myogenin (MYOG) expression corresponding with the slight change in cell morphology. (nih.gov)
  • DDAH2kd by siRNA showed a 40% reduction in mRNA expression with a slight change in cell morphology (Figure 5A&B). Interestingly, DDAH2kd showed a decrease in MYOG upto 50% during myogenesis (Figure 5A). (nih.gov)
  • Plectin isoform P1b and P1d deficiencies differentially affect mitochondrial morphology and function in skeletal muscle. (abcam.com)
  • All colonies showed characteristic morphology of muscle lineage cells. (nih.gov)
  • Harvard Stem Cell Institute
  • After more than two years of intensive ethical and scientific review, Harvard Stem Cell Institute (HSCI) researchers at Harvard and Children's Hospital Boston have been cleared to begin experiments using Somatic Cell Nuclear Transfer (SCNT) to create disease-specific stem cell lines in an effort to develop treatments for a wide range of now-incurable conditions afflicting tens of millions of people. (rxpgnews.com)
  • human
  • However, there is an ethical consensus in the United States not to allow preimplantation human embryos to develop beyond 80 to 100 cells, about the size of a pinhead. (innovations-report.com)
  • HLA-A2/IGRP tetramer staining revealed a higher frequency of IGRP-specific CD8 T-cells in the peripheral blood of recent onset human individuals than of healthy donors. (jove.com)
  • Even later, at the blastocyst stage, only 20-25% of human embryos consist of cells with a normal karyotype. (iliveok.com)
  • Placental Stem Villus Arterial Remodeling Associated with Reduced Hydrogen Sulfide Synthesis Contributes to Human Fetal Growth Restriction. (abcam.com)
  • 2 But public attention has recently increased with a new milestone that has been reached: the production of human embryonic stem cells. (lifeissues.net)
  • Mouse anti Human CD29 antibody, clone 4B7R used for the evaluation of integrin β1 expression on human muscle derived cells by flow cytometry and immunofluorescence microscopy. (bio-rad-antibodies.com)
  • Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. (bio-rad-antibodies.com)
  • The role of telomeres and telomerase in cell aging and cancer was established by scientists at biotechnology company Geron with the cloning of the RNA and catalytic components of human telomerase and the development of a polymerase chain reaction (PCR) based assay for telomerase activity called the TRAP assay, which surveys telomerase activity in multiple types of cancer. (wikipedia.org)
  • For instance, multilineage-differentiating stress-enduring (Muse) cells are stress-tolerant adult human stem cells that can self-renew. (wikipedia.org)
  • hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
  • This isoform is expressed exclusively in heart muscle during human and mouse development, and is distinct from those expressed in slow skeletal muscle (MYBPC1) and fast skeletal muscle (MYBPC2). (wikipedia.org)
  • The cloning of the human MYBPC3 cDNA and localization of the gene on human chromosome 11p11.2 has assisted the structure and function of cMyBP-C. MYBPC3 became therefore the "best" candidate gene for the CMH4 locus for hypertrophic cardiomyopathy that was initially mapped by the group of Schwartz. (wikipedia.org)
  • Can be isolated as cells positive for SSEA-3, a well known human embryonic stem cell marker. (wikipedia.org)
  • Muse cells are identified as cells positive for SSEA-3+, a well-known marker for undifferentiated human ES cells. (wikipedia.org)
  • showed that human dermal fibroblast-derived Muse cells were efficiently differentiated into melanin-producing functional melanocytes by a cocktail of cytokines. (wikipedia.org)
  • This was shown in human Muse cells infused into animal models with fulminant hepatitis, partial hepatectomy, muscle degeneration, skin injury, stroke and spinal cord injury. (wikipedia.org)
  • The human immune system typically produces both T cells and B cells that are capable of being reactive with self-antigens, but these self-reactive cells are usually either killed prior to becoming active within the immune system, placed into a state of anergy (silently removed from their role within the immune system due to over-activation), or removed from their role within the immune system by regulatory cells. (wikipedia.org)
  • lineage
  • The step-wise maturation of stem cells into terminally differentiated cell types requires the timely activation of a cascade of transcription programs governed by lineage-specifying transcription factors. (stembook.org)
  • Plasticity implies that stem cells can transdifferentiate into mature cell types outside their original lineage in response to microenvironmental cues. (ahajournals.org)
  • A muse cell (multi-lineage differentiating stress enduring cell) is a endogenous non-tumorigenic pluripotent stem cell. (wikipedia.org)
  • C2C12
  • Furthermore, HBA2 silencing in C2C12 cells showed an upto 70% reduction in its expression during transdifferentiation (Figure 6A). (nih.gov)
  • therapeutic
  • Somatic cell nuclear transfer ("therapeutic cloning"), which is one way to derive stem cells, shows potential in generating functional replacement cells such as insulin-producing cells associated with diabetes. (innovations-report.com)
  • Thus, studies of pathogenesis and therapeutic interventions in T1D can proceed at a faster rate than with methods that rely on heterogenous T cell populations or clones derived from diabetic NOD mice. (jove.com)
  • However, in addition to being technically difficult and cumbersome, such cell-based approaches are highly sensitive to cytotoxic T cell responses, which limits their therapeutic capacity. (jove.com)
  • It had become clear that the therapeutic use of ES cells, as such, entailed significant risks, since - as had been observed in experiments on mice - tumours resulted. (lifeissues.net)
  • peripheral
  • IGRP(265-273)-specific CD8 T-cells that were cloned from the peripheral blood of a recent onset T1D individual were shown to secrete IFN? (jove.com)
  • expression
  • 2017). Functional Expression of P-glycoprotein and Organic Anion Transporting Polypeptides at the Blood-Brain Barrier: Understanding Transport Mechanisms for Improved CNS Drug Delivery? (tcdb.org)
  • A polymorphism in the promoter region of the NDFUB6 gene resulting in an adenine to guanine shift at rs629566 was shown to create a DNA methylation site that is associated with a decline in NDUFB6 expression in muscle of aging patients. (wikipedia.org)
  • In situ hybridization revealed Tnmd expression in various parts of the adult mouse brain such as the dentate gyrus, CA regions of the hippocampus, neurons in the cerebral nuclei, cerebellum, Purkinje cells and neuronal cells in the cerebellar nucleus. (wikipedia.org)
  • An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
  • The expression of CD97 on macrophage and the abundant presence of its ligand CD55 on fibroblast-like synovial cells suggest that the CD97-CD55 interaction is involved in the recruitment and/or retention of macrophages into the synovial tissue in rheumatoid arthritis. (wikipedia.org)
  • Ectopic CD97 expression upregulates the expression of N-cadherin and β-catenin in HT1080 fibrosarcoma cells leading to enhanced cell-cell aggregation. (wikipedia.org)
  • DACH1 inhibits Cyclin D1 expression and thereby reduces breast cancer cell line cell growth. (wikipedia.org)
  • Modulation of K+ channel activity and expression has been found to be at the crux of many profound pathophysiological disorders in several cell types. (wikipedia.org)
  • fibroblasts
  • Katayama M, Hirayama T, Kiyono T, Onuma M, Tani T, Takeda S, Nishimori K, and Fukuda T: Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. (go.jp)