Functional replacement cells skeletal muscle stem cells clone cells. Medical search. Frequent questions

They may instead be fusing with existing cells, creating genetically mixed-up tissues with unknown health effects" [1]. (i-sis.org.uk)
confirming that pecans modulate adipose tissues lipolysis and mitochondrial oxidative metabolism in the liver and skeletal muscle. (worldhealth.net)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
Increased curiosity about CAPN3 was highly stimulated when it had been reported that mutations in its gene bring about limb-girdle muscular dystrophy (LGMD) type 2A, seen as a the continuous atrophy of hip and make muscle tissues (LGMD2A, or calpainopathy) (21). (exposed-skin-care.net)
Background: An increasing number of studies on stem cells suggests that the therapeutic effect they exert is primarily mediated by a paracrine regulation through extracellular vesicles (EVs) giving solid grounds for stem cell EVs to be exploited as agents for treating diseases or for restoring damaged tissues and organs. (123dok.org)
In addition, very recent data indicate that the structural contribution of stem cells (SCs) to regenerated tissues seems to be limited, and that rather the stimulation of local healing processes plays an important role in regeneration [6]. (123dok.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Most CARs that are being evaluated in current clinical and preclinical studies recognize self-antigens that are expressed by normal tissues as well as malignant cells. (ashpublications.org)

The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)

In 1989, she completed her Ph.D. in Cell & Molecular Biology, and worked as a Research Scientist at the Cardiology Centre studying vascular smooth muscle involvement in atherosclerosis. (wikipedia.org)
Professor Elena Cattaneo is Director of the Laboratory of Stem Cell Biology and Pharmacology of Neurodegenerative Diseases at the Department of Pharmacological Sciences, as well as a co-founder and first appointed Director of UniStem , the Centre for Stem Cell Research of the University of Milano. (eurostemcell.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
2005 (October 1- December 15) Visiting Scientist Harvard Medical School, Joslin Diabetes Centre, Section of Islet Transplantation and Cell Biology, Boston/USA. (livhospital.com)
Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)

Cystic fibrosis (CF) is an autosomal recessive genetic disease characterized by the hypersecretion of mucus by specialized epithelial cells in the lungs. (healthdrugpdf.com)
It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. (lookformedical.com)

The canonical Wnt signaling pathway is a key regulator of mesenchymal stem cell differentiation into either osteoblasts or adipocytes with active Wnt signaling promoting osteoblastogenesis. (bvsalud.org)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)

Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)

Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
This has led to the need for new immunotherapy-based approaches, including adoptive cell transfer (ACT), therapeutic vaccines, and novel immune checkpoint inhibitors. (biomedcentral.com)
This has resulted in the development of additional immunotherapy-based approaches, including adoptive cell transfer (ACT), therapeutic vaccines, and inhibitors of other immune checkpoints. (biomedcentral.com)

This latest anti-publicity on adult stem cells comes on the heels of a paper announcing success in embryonic stem (ES) cell transplant in a Parkinson rat model published in the house journal of the United States National Academy of Sciences [5]. (i-sis.org.uk)
In the current issue of the same journal [6], researchers compared the frequency and type of mutation induced in embryonic stem cells and embryonic somatic cells. (i-sis.org.uk)
They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Due to their capacity to differentiate in all embryonic germ layers, amnioticﬂuid stem cells (AFCs), represent a highly promising cell type for tissue regeneration, which however is still poorly studied and in turn underutilized. (123dok.org)

Since germ therapy is too late for everyone alive, we have to correct the genotype of a significant number of our somatic cells or replace them by new ones. (senescence.info)

We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)

In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
201T and CALU-3 airway tumor cells were grown to confluency in cell culture plates and Transwell (Costar) filters, respectively, at 37°C in Dulbecco's Modified Eagle's medium with 15% fetal bovine serum. (healthdrugpdf.com)
One is the ability to administer large numbers of highly selected cells with high avidity for tumor antigens that can recognize shared and patient-specific mutated (neo) antigens. (biomedcentral.com)
Successful TIL therapy starts with the identification, enrichment, stimulation, and expansion of potent tumor-reactive T cells. (biomedcentral.com)
Herein we used a single bicistronic lentiviral vector to develop a new combination immunotherapy that consists of human anti-carbonic anhydrase IX (CAIX)-targeted chimeric antigen receptor (CAR) T cells engineered to secrete human anti-programmed death ligand 1 (PD-L1) antibodies at the tumor site. (oncotarget.com)
Tumor growth diminished 5 times and tumor weight reduced 50-80% when compared with the anti-CAIX CAR T cells alone in a humanized mice model of ccRCC. (oncotarget.com)
The anti-PD-L1 IgG1 isotype, which is capable of mediating ADCC, was also able to recruit human NK cells to the tumor site in vivo . (oncotarget.com)
As an evasion mechanism, many tumors are able to stimulate the expression of immune checkpoint molecules, resulting in an exhausted phenotype of T cells that cannot restrain tumor progression [ 1 ]. (oncotarget.com)
Non-Vascularised Ipsilateral Fibular strut - A Modality to Treat Giant cell tumor of lower end radius using Anterior Approach. (jbstjournal.com)
Giant cell tumor is a benign bone tumor, locally aggressive with low malignant potential. (jbstjournal.com)
Juxta-articular giant cell tumors of the lower end radius are common and present a special problem of reconstruction after tumor excision. (jbstjournal.com)
Giant cell tumor (GCT) of bone is a benign but locally aggressive tumor with tendency for local recurrence [2]. (jbstjournal.com)
We studied 5 consecutive cases of GCT involving the distal radius operated by en-bloc resection of tumor followed by reconstruction with ipsilateral non-vascularized fibular graft with a minimum 1 year follow-up. (jbstjournal.com)

The main research theme of her lab is neural stem cells, and the molecular pathophysiology of Huntington's Disease . (eurostemcell.org)

Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)

Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Those cells show high plasticity and the capacity to differentiate into the three types of germ layer cells, making them ideal for ap-plications in regenerative medicine[4], eventually banked and used for treatments. (123dok.org)
Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)

Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)

Two reports appeared as advance online publications in the top British journal Nature , accompanied by a news report that begins, "The hyped ability of adult stem cells to sprout replacement tissue types is being called into question. (i-sis.org.uk)
It is hypothesized that prednisone reduces tissue inflammation, suppresses cytotoxic cells, improves calcium homeostasis, and stimulates myoblasts. (medscape.com)
It is unknown whether the spatiotemporal coupling between myofiber and microvascular regeneration persists when muscle tissue is completely removed and local guidance cues are lost. (biomedcentral.com)
To test whether complete removal of skeletal muscle tissue affects the spatiotemporal relationship between myogenesis and angiogenesis during regeneration, subthreshold volumetric muscle loss was created with a biopsy punch (diameter, 2 mm) through the center of the gluteus maximus (GM) in adult mice. (biomedcentral.com)
In contrast to preserving residual guidance cues following myotoxins or physical trauma, volumetric muscle loss (VML) removes all tissue components. (biomedcentral.com)
2017-Present Istinye University Stem Cell and Tissue Engineering RD Manager. (livhospital.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
A cell-based tissue-engineered urethra may be an alternative for patients who have complicated long strictures and need urethral reconstruction. (biomedcentral.com)
Autologous USCs can be used as an alternative cell source for cell-based tissue engineering for urethral reconstruction. (biomedcentral.com)
Another critical element required for successful urethral tissue engineering is the cell source. (biomedcentral.com)
Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. (lookformedical.com)

In addition to fully differentiated cells, precursors and multipotent stem like cells have been described within AF[3]. (123dok.org)

We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)

Prime editors (PEs) are precision gene editing agents that can perform virtually any substitution, small deletion and small insertion at target DNA sites in living cells 2 . (nature.com)
Fasting influences the circadian clock and fasting driven cellular responses which work together to achieve fasting specific temporal gene regulations, such as skeletal muscle appears to be twice as responsive to fasting as is the liver, explains Paolo Sassone-Corsi who first showed the circadian rhythm metabolism link some 10 years ago, identifying metabolic pathways through which circadian proteins sense cell energy levels. (worldhealth.net)
The gene and cell approaches are more likely to be curative, but they are still under investigation. (medscape.com)
Changes in gene expression can cause defects in myogenesis and muscle regeneration, apoptosis, and sensitivity to oxidative stress. (comprehensivephysiology.com)
As opposed to other styles of muscles dystrophy, where mutations take place in genes encoding structural protein, calpainopathy was the initial reported kind of dystrophy predetermined by mutations within a gene encoding a proteolytic enzyme. (exposed-skin-care.net)
Clonal plasma cell proliferative diseases in which the Vl 6 gene is expressed are always associated with amyloid deposition. (medscape.com)

CD19 expression is restricted to normal mature B cells, malignant B cells, and B-cell precursors. (ashpublications.org)

To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)

We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
Until these molecular therapies become clinically available, supportive therapies can be used to protect muscle mass and function and to help improve quality of life. (medscape.com)
The use of cell therapies, drivers of immune response, and trends in immunotherapy were the focus of the Immunotherapy Bridge (November 30th-December 1st, 2022), organized by the Fondazione Melanoma Onlus, Naples, Italy, in collaboration with the Society for Immunotherapy of Cancer. (biomedcentral.com)
The use of cell therapies, drivers of immune response, and trends in immunotherapy were the focus of the Immunotherapy Bridge (November 30th-December 1st, 2022). (biomedcentral.com)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)

But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
Cloning and characterisation of stage specific genes from clinical isolates of Leishmania donovani. (icmr.nic.in)
In most cases, the disease is autosomal dominantly inherited and the patients often have a mutation in genes encoding the muscle contraction apparatus. (biomedcentral.com)

PAPSS1 inhibition in combination with low-dose (IC 10 ) cisplatin resulted in increased DNA damage, apoptosis and G1/S cell cycle arrest. (oncotarget.com)
15 In addition to promoting T-cell activation, the first and third ITAMs of the CD3-ζ molecule have been shown to cause apoptosis, and inactivation of the first and third ITAMs of the CD3-ζ molecule by converting their tyrosine residues to phenylalanines has been shown to decrease T-cell apoptosis. (ashpublications.org)

The muscle biopsy with protein analysis can be valuable in this determination. (medscape.com)
Following punch biopsy of adult skeletal muscle, regenerating microvessels span the wound and become perfused with blood prior to myofiber regeneration. (biomedcentral.com)
Therefore, as an alternative approach to test the interdependence of microvascular and myofiber regeneration following acute injury, a punch biopsy (diameter, 2 mm) was performed through the mouse gluteus maximus (GM) muscle. (biomedcentral.com)

Evidence has been found by University of California scientists of fasting affecting circadian clocks in the liver and skeletal muscle causing them to rewire their metabolism, which can lead to improved health and protection against aging associated diseases, as published in Cell Reports. (worldhealth.net)
Initial, CAPN3 is AZ-33 normally mostly muscle-specific (9), but is normally detectable in zoom lens, liver, human brain and cardiac muscles during advancement (10C13). (exposed-skin-care.net)
A primary malignant neoplasm of epithelial liver cells. (lookformedical.com)
Lipid infiltration of the hepatic parenchymal cells resulting in a yellow-colored liver. (lookformedical.com)

The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)

Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)

Immunoglobulin-related amyloidosis is a monoclonal plasma cell disorder in which the secreted monoclonal immunoglobulin protein forms insoluble fibrillar deposits in one or more organs. (medscape.com)
Before the discovery that the major fibril component in these patients was an immunoglobulin fragment, patients with light chain-type amyloidosis were described as having primary (in the sense of idiopathic) amyloidosis or, when the burden of monoclonal plasma cells was large, myeloma-associated amyloidosis. (medscape.com)
These monoclonal plasma cell disorders can be categorized according to the total body burden of monoclonal plasma cells. (medscape.com)
In most patients with a monoclonal plasma cell disorder, whether multiple myeloma or MGUS, the monoclonal L chain secreted by the clone remains soluble in the bloodstream. (medscape.com)
The relationship among light chain-type amyloidosis (AL), the other monoclonal plasma cell disorders, and the other amyloidoses. (medscape.com)
Anti-CD19-CAR-transduced T cells had superior antilymphoma efficacy compared with the anti-CD19 monoclonal antibody from which the anti-CD19 CAR was derived. (ashpublications.org)

congestive care: external, weak division that invades known with the extracellular muscle which is in human or elderly thrombosis. (siriuspixels.com)

Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

Adoptive T-cell therapy with anti-CD19 chimeric antigen receptor (CAR)-expressing T cells is a new approach for treating advanced B-cell malignancies. (ashpublications.org)

b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)

In the latest skirmish, two rapid online publications are presented as if they were evidence against the existence of adult stem cells. (i-sis.org.uk)
This leaves one with the distinct impression that adult stem cells might not exist, and furthermore, they may be a health hazard. (i-sis.org.uk)
Fusion could also be obtained between ES cells and cells isolated from adult brain. (i-sis.org.uk)
These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
In fact, the senior authors in both reports admit that some adult stem cells may genuinely revert to an earlier stage of development or switch fates [1]. (i-sis.org.uk)
The finding that adult cells can fuse with ES cells is therefore irrelevant to the debate, and the fact that it was used in an attempt to discredit adult stem cells is itself revealing, particularly in view of other recent findings on ES cells. (i-sis.org.uk)

A) Heat map of fat infiltration scores of muscles from 13 FSHD subjects, rated on a scale of 0 (white, least infiltrated) to 5 (red, most infiltrated), as assessed by T1‐weighted MRI. (comprehensivephysiology.com)
Moreover, inflammatory cell infiltration and fibrosis were found in the control group with only SIS, but not in the experimental autologous USC-seeded SIS group. (biomedcentral.com)

A recent report 35 demonstrates efficient in vivo prime editing in enriched degenerating retinitis pigmentosa mouse retinas with dual-AAV delivery of PE-SpRY, but this system may not be applicable to uses that do not confer a survival advantage among edited cells. (nature.com)
These cells can be activated ex vivo to exhibit a potent antitumor effector function. (biomedcentral.com)
The study and/or manipulation of administered cells can potentially result in identification of the exact cell subpopulations and effector functions required for cancer regression in vivo. (biomedcentral.com)

Mutations in the DSL (Delta, Serrate, Lag2) Notch (N) ligand Delta-like (Dll) 3 cause skeletal abnormalities in spondylocostal dysostosis, which is consistent with a critical role for N signaling during somitogenesis. (rupress.org)
However, the functional consequences of the two mutations are distinct, with loss of axonal transport of ECEL1/DINE in C760R mutants and mRNA expression deficits in G607S mutants. (biomedcentral.com)

Human muscle fibers identified by anti‐human spectrin immmunostaining. (comprehensivephysiology.com)
In future studies, CFTR expression will be down-regulated in CALU-3 cells, and primary cultures of CF and non-CF human airway cells will be used to define the role of CFTR in the intracellular processing of MUC1. (healthdrugpdf.com)
Clinical activity of TIL therapy has also been reported in other cancer types, including human papillomavirus-associated cancers, cholangiocarcinoma, non-small-cell lung cancer (NSCLC), and triple-negative breast cancer (TNBC). (biomedcentral.com)
These armed second-generation CAR T cells empowered to secrete human anti-PD-L1 antibodies in the ccRCC milieu to combat T cell exhaustion is an innovation in this field that should provide renewed potential for CAR T cell immunotherapy of solid tumors where limited efficacy is currently seen. (oncotarget.com)
15 We have recently conducted experiments that demonstrated enhanced in vitro survival of human T cells that were transduced with an ErbB2-specific CAR when the first and third ITAMs of the CD3-ζ domain of the CAR were inactivated. (ashpublications.org)

2014-Present Professor Liv Hospital (Ulus) Regenerative Medicine Stem Cell Production Center Istanbul- Turkey. (livhospital.com)
The rapid advances in theﬁeld of regenerative medicine have identiﬁed novel sources of stem cells with potential for therapy. (123dok.org)

However, the effective clinical translation of AFCs based ap-proaches requires in vitro culture expansion to achieve a sufﬁcient number of cells and strict characterization of the cultures, two factors that may severely hamper their clinical use[5]. (123dok.org)
12 , 13 Clinical trials in which patients with advanced B-cell malignancies receive T cells expressing anti-CD19 CARs are in early stages, and it is not known whether adoptive transfer of T cells targeting this self-antigen will be an effective therapy for B-cell malignancies. (ashpublications.org)
Giant cell tumors (GCT) of the bone are aggressive and are recognised for variable clinical behaviour, which is not always related to radiographic or histological appearance [1]. (jbstjournal.com)

Cell therapy uses myoblast and stem cell techniques. (medscape.com)
T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)
To evaluate anti-CD19-CAR-transduced T cells in a murine model of adoptive T-cell therapy, we developed a CAR that specifically recognized murine CD19. (ashpublications.org)
Adoptive T-cell therapy with T cells expressing chimeric antigen receptors (CARs) is an active area of cancer research. (ashpublications.org)

To establish a murine model in which a completely syngeneic lymphoma could be treated by adoptive transfer of syngeneic CAR-transduced T cells, we developed a CAR that could specifically recognize murine CD19. (ashpublications.org)

In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
DUX4‐fl transcription factor misexpression in skeletal muscle leads to disrupted RNA splicing, nonsense mediated decay (NMD), and protein degradation, possibly causing damaged associated molecular pattern (DAMP) release, which may elicit an immune response to targeted damaged muscles. (comprehensivephysiology.com)
Also, other DUX4‐fl protein interactions may affect muscle function. (comprehensivephysiology.com)
Furthermore ORP-150, a novel protein that is expressed by macrophages under ischemic conditions, is likely to be critical in enabling these cells to redirect their biosynthetic activities in hypoxia. (healthdrugpdf.com)
To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. (nature.com)
These transitions are enacted by profound changes in protein composition of the cells. (nature.com)
The combined proteomic approach called PISA-Express implemented here uses just two samples per replicate analysis of a cell type (Fig. 1a, b ) to assess both the protein abundance and solubility. (nature.com)
Fig. 1: Protein thermal stability and expression changes in cell-type transitions. (nature.com)
Conclusions: Our study demonstrates for theﬁrst time the expression of the full protein hTERT by AFCs and its release outside the cell mediated by EVs, indicating a new extra telomeric role for this protein. (123dok.org)
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)

J Cell Biol (2005) 170 (6): 983-992. (rupress.org)

When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
Structural studies of cell transition in pathogenesis of mycobacterial diseases. (icmr.nic.in)

Western blot analysis of treated CALU-3 cells using an antibody against the MUC1 ectodomain suggested that MUC1 expression decreased with exposure to brefeldin A and monensin. (healthdrugpdf.com)

We used T cells that were retrovirally transduced with this CAR to treat mice bearing a syngeneic lymphoma that naturally expressed the self-antigen murine CD19. (ashpublications.org)
One infusion of anti-CD19-CAR-transduced T cells completely eliminated normal B cells from mice for at least 143 days. (ashpublications.org)
The antilymphoma efficacy of anti-CD19-CAR-transduced T cells was critically dependent on irradiation of mice before anti-CD19-CAR-transduced T-cell infusion. (ashpublications.org)

Metabolic reprogramming in these cells not only supports phenotypic changes, but also provides the necessary energy for their highly energy-consuming activity, bone resorption. (bvsalud.org)
RECENT FINDINGS: Osteoclasts undergo metabolic reprogramming to meet the energy demands during their transition from precursor cells to fully mature bone-resorbing osteoclasts. (bvsalud.org)
Campanacci's staging system for giant cell tumour of the bone [5] was used for cortical breach. (jbstjournal.com)

1 , 2 , 14 Phosphorylation of tyrosines in immunoreceptor tyrosine-based activation motifs (ITAMs) of CD3-ζ molecules is important for T-cell activation. (ashpublications.org)

We conclude that angiogenesis precedes myogenesis during regeneration following subthreshold volumetric muscle loss. (biomedcentral.com)
In addition, the urethral caliber, speed of urothelial regeneration, content of smooth muscle, and vessel density were significantly improved in the group with autologous USC-seeded SIS. (biomedcentral.com)

determinants ON CONGESTIVE HEART FAILURE Overview In this download the roots, we are you how to be ischaemic on myocytes assignments of muscle on ALTERNATIVE heart signal. (siriuspixels.com)

Immunoreceptor signaling during development, homeostasis, and effector function of T cells and NK cells. (upstate.edu)

DUX4 expression was visualized in differentiated 17ABic myogenic cells (104) using an integrated DUX4‐GFP reporter (198). (comprehensivephysiology.com)
Interestingly, stimulation of CALU-3 cells with forskolin, a cAMP agonist, slightly increased MUC1 surface expression and decreased MUC1 release. (healthdrugpdf.com)
To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
The expression of PD-L1 and Ki67 in the tumors decreased and an increase in granzyme B levels was found in CAR T cells. (oncotarget.com)

Transferred T cells that persist in the blood and form memory have a phenotype consistent with a less differentiated state (CD27/CD28/IL-7 receptor-α) [ 3 ]. (biomedcentral.com)

Our results demonstrated impressive antilymphoma activity and profound destruction of normal B cells caused by anti-CD19-CAR-transduced T cells in a clinically relevant murine model. (ashpublications.org)

Nonetheless, it appears that in order to cure aging we will need to target multiple types of cells and possibly address different types of molecular damage and malfunction. (senescence.info)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)

Inflammation is implicated in the pathogenesis of the dystrophinopathies despite the fact that most biopsies in patients with Duchenne muscular dystrophy do not show inflammatory cells. (medscape.com)

Various reconstructive procedures described, non-vascularised fibular autograft has been widely used with satisfactory functional results. (jbstjournal.com)

Anatomy43

Organisms8

Diseases5

Chemicals and Drugs19

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes25

Disciplines and Occupations2

Information Science3

Tissues9

Endothelial3

Biology7

Epithelial cells2

Mesenchymal stem1

Hematopoietic2

Differentiation15

Therapeutic6

Embryonic5

Somatic cells1

Pluripotent stem1

Tumor13

Neural1

Progenitor2

Differentiate4

Stromal cells2

Tissue12

Multipotent1

Maturation1

Gene6

Precursors1

Self-renewal1

Therapies6

Genes3

Apoptosis2

Biopsy3

Liver4

MSCs8

Abstract1

Monoclonal6

Extracellular1

Transplantation2

Chimeric antigen receptor1

Proteins2

Adult6

Infiltration2

Vivo3

Mutations2

Human5

Regenerative medicine2

Clinical3

Therapy4

Adoptive1

Protein10

Biol1

Diseases2

Antibody1

Mice3

Bone3

Molecules1

Regeneration2

Myocytes1

Effector function1

Expression4

Phenotype1

Clinically relevant1

Molecular2

Inflammatory1

Widely1