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  • hPSC
  • The researchers were successful in producing contractile sheets consisting of up to 95% TNNT2 + cardiomyocytes, in high yield (up to 100 cardiomyocytes per hPSC), and from 11 different cell lines. (invitria.com)
  • Here, we discuss the progress and impediments the field has faced in using hPSC-derived cardiomyocytes for these in vitro applications, and highlight that rigorous protocol optimisation and standardisation, scalability and automation are remaining obstacles for the generation of pure, mature and clinically relevant hPSC cardiomyocytes. (springer.com)
  • electrophysiology
  • The most promising 3 polymers were scaled up for extended culture of hESC-CMs for 15 days and were characterized using patch clamp electrophysiology and myofibril analysis to find that functional and structural phenotype was maintained on these synthetic substrates without the need for coating with extracellular matrix protein. (imperial.ac.uk)
  • efficacy
  • iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
  • efficiently differentiated
  • In this study we show that forced aggregation of a defined number of single cells on AggreWell plates gives a high number of homogeneous EBs that can be efficiently differentiated into functional CMs by application of defined growth factors in the media. (springer.com)
  • Moreover, the isolated cells could be the efficiently differentiated into functional cardiomyocytes in optimized conditions. (hindawi.com)
  • enhances
  • Injection of the COX‐2 product PGE 2 enhances cardiomyocyte replenishment in young mice and recovers cell renewal through attenuating TGF‐β1 signaling in aged mice. (embopress.org)
  • characterize
  • This study aimed to characterize β 3 -adrenergic receptors (ARs) in rat neonatal cardiomyocytes using the noradrenaline (NOR) properties to modulate the expression and function of the three β-ARs. (aspetjournals.org)
  • Genetic
  • This problem has been, at least partially, overcome by utilizing clever labelling schemes, which rely on the intentional or non-intentional labelling of the genetic material of cardiomyocytes. (intechopen.com)
  • cell cycle
  • Interestingly, a recent study by Senyo et al reported that ~20% of pre‐existing cardiomyocytes at the border zone undergo cell cycle although among them, only 3.2% of cells complete the cell division (Senyo et al , 2013 ). (embopress.org)
  • A very low but nevertheless detectable level of cardiomyocyte cell cycle activity was reported in rodent studies almost 50 years ago ( Soonpaa & Field, 1998 ). (intechopen.com)
  • conditions
  • however, this process is inefficient under serum-based culture conditions, in which conversion of partially reprogrammed cells into fully reprogrammed functional iCMs has been a major hurdle. (semanticscholar.org)
  • The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)
  • mice
  • Wild-type and cardiomyocyte mineralocorticoid receptor knockout mice (8 weeks) were uninephrectomized and maintained on (1) high salt (0.9% NaCl, 0.4% KCl) or (2) high salt plus deoxycorticosterone pellet (0.3 mg/d, 0.9% NaCl, 0.4% KCl). (ahajournals.org)
  • PGE2 rescues the cardiomyocyte replenishment capacity that is lost in aged mice. (embopress.org)
  • responses
  • Responses mediated by agonists specific for P2Y receptors subtypes showed that P2Y receptors (P2Y 1 , P2Y 2 , P2Y 4 and P2Y 6 ) were also present in both E14 and E18 cardiomyocytes. (springer.com)