Functional adult rat cardiomyocytes. Medical search. Frequent questions

Expression of a mutant form of Cadherin-2 harboring a large deletion in the extracellular domain inhibited the function of endogenous Cadherin-2 in adult ventricular cardiomyocytes, and neighboring cardiomyocytes lost cell-cell contact and gap junction plaques as well. (wikipedia.org)
Isolated adult rat ventricular cardiomyocytes were saponin-permeabilised and incubated with each peptide. (whiterose.ac.uk)
In addition, In vitro cell culture models will be studied to address the underlying cellular mechanisms by utilizing: 1) Adult mouse cardiac fibroblasts (F2KO and WT) and 2) Neonatal rat ventricular cardiomyocytes. (centerforpediatricresearch.org)
Although ventricular cardiomyocytes express inositol 1,4,5-trisphosphate [Ins(1,4,5) P 3 ] receptors, it is unclear how these Ca 2+ channels contribute to the effects of Gq-coupled agonists. (biologists.com)

A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)
The research also studied the differentiation capacity into cardiomyocytes. (romanianjournalcardiology.ro)
Adult human mesenchymal stem cells can recellularize the matrix with reduce efficiency, while the iPSC could have a better grafting and cardiac differentiation rate. (romanianjournalcardiology.ro)
Here we show that changes in the composition of the extracellular matrix during this week can affect cardiomyocyte growth and differentiation in mice. (nature.com)
There are areas in the cardiac muscle with anatomical and functional differentiation that present automatism, thus subjecting the rest of the fibers to their own rhythm. (bvsalud.org)

Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
Older cardiospheres developed foci of "beating" cells upon co-culture with rat neonatal cardiomyocytes. (bvsalud.org)

Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
The objective of this study was to evaluate the ability of human iPSC-CMs to improve myocardial function in a rat postinfarction heart failure model. (biomedcentral.com)
Human iPSC-CM grafts survived in infarcted rat hearts and restored myocardial function 4 weeks after transplantation. (biomedcentral.com)
Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)
Qian Y, Qian F, Zhang W, Zhao L, Shen M, Ding C, Guo J ( 2019 ) Shengjiang Powder ameliorates myocardial injury in septic rats by downregulating the phosphorylation of P38-MAPK. (multichannelsystems.com)
In vivo , a single administration of agrin promotes cardiac regeneration in adult mice after myocardial infarction, although the degree of cardiomyocyte proliferation observed in this model suggests that there are additional therapeutic mechanisms. (nature.com)
Cardiomyocyte hypertrophy is a mechanism by which myocardial mass is increased to compensate for any elevated physical demands placed upon the heart, thus ensuring that adequate perfusion of body tissues is maintained during these periods. (europeanpharmaceuticalreview.com)
One of the major factors contributing to myocardial de-compensation is cardiomyocyte energy starvation2. (europeanpharmaceuticalreview.com)
Upregulation of Fas expression in myocardial ischemia-reperfusion can induce cardiomyocyte apoptosis, and atorvastatin can significantly inhibit cardiomyocyte apoptosis by inhibiting Fas expression. (ac.ir)
Apoptosis of the cardiomyocytes has been demonstrated in animal models with coronary artery occlusion [ 1 ], and experimental evidence suggests that myocardial cells are able to undergo apoptosis during ischemia followed by reperfusion [ 2 ]. (ac.ir)
It has been shown that the Fas pathway is functional in cardiac myocytes and plays a critical role in myocardial death due to ischemia-reperfusion in vivo [ 4 ]. (ac.ir)
It was shown that functional Fas system contributes to apoptotic myocardial cell death in response to ischemia/reperfusion injury [ 4 , 5 ]. (ac.ir)

To obtain functional cardiac tissue on a rat animal model, starting with a decellularized heart used as a matrix for grafting the human cells- adult mesenchymal stem cells (MSC) and induced pluripotent stem cells (iPSC) obtained through peripheral blood lymphocytes T transduction. (romanianjournalcardiology.ro)
Analysis of peripheral nerve regeneration after transection on the model of rat caudal nerve using tubulization and mesenchymal stem cells. (sav.sk)

Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
In this study, we describe the formation of three-dimensional (3D) cell aggregates ("cardiospheres") by putative stem cells isolated from adult dog myocardium using poly-L-ornithine. (bvsalud.org)
These findings suggest that cardiospheres are potentially useful for delivering functional stem cells to the damaged heart. (bvsalud.org)
In vitro , recombinant agrin promotes the division of cardiomyocytes that are derived from mouse and human induced pluripotent stem cells through a mechanism that involves the disassembly of the dystrophin-glycoprotein complex, and Yap- and ERK-mediated signalling. (nature.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
In addition, the authors also review future strategies in DN treatment such as transcriptional reprogramming of mature adult kidney cells into uncommitted induced pluripotent stem cells for renal repair and therapeutics. (emjreviews.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

Human and rat hearts are composed of 66% cardiac fibroblasts (CF) 30 cardiomyocytes (CM) and 4% endothelial and vascular smooth muscle cells5-7. (abt-888.net)

Eight-week-old male Sprague-Dawley rats were randomly selected to receive an intramyocardial injection of 5% albumin solution with or without 1 × 10 7 human iPSC-CMs 10 days after undergoing left anterior descending (LAD) coronary artery ligation. (biomedcentral.com)
In vivo fractional MMP ooccupancy of VS-041 was determined based on unbound fraction (Fu) plasma exposure in Sprague Dawley rats after oral administration at 50 mg/kg. (syncrosome.com)

Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)

We were interested in the developmental potential of single cardiac progenitor cell into cardiomyocytes, smooth muscle cells, and endothelial cells. (stanford.edu)

Three-four-week old (on arrival) Dahl/SS male rats (SS/JrHsdMcwiCrl, Charles River, US), were used for this study. (syncrosome.com)
Flutamide (50 mg/kg body weight) was administered to male rats daily from 82 to 88 postnatal day. (biomedcentral.com)

Dox-induced over-production of reactive oxygen and nitrogen species (ROS and RNS) such as superoxide, hydrogen peroxide, hydroxyl radical and peroxynitrite has been proposed to be the main cause of Dox-induced acute cardiomyocyte toxicity and cell death by damaging various molecular constituents and organelles [ 9 - 12 ]. (oncotarget.com)
Chronic overexpression of MT or acute in vitro treatment with the flavoprotein inhibitor diphenyleneiodonium or the angiotensin II type I receptor antagonist losartan eliminated excess ROS production in diabetic cardiomyocytes. (diabetesjournals.org)
Therefore, we designed this study to see the influence of atorvastatin on cardiomyocyte apoptosis and Fas expression following acute I/R in vivo. (ac.ir)
Thirty Wistar rats were selected and divided into three groups (n = 10): acute ischemia-reperfusion (I/R) group, acute ischemia-reperfusion and treated with atorvastatin group and sham-operated group. (ac.ir)

During postnatal development the heart undergoes a rapid and dramatic transition to adult function through transcriptional and post-transcriptional mechanisms including alternative splicing (AS). (abt-888.net)

Both ischemic and reperfused rat myocardium can undergo apoptotic cell death, however the myocardium, which is subjected to ischemia followed by reperfusion, undergoes accelerated apoptosis [ 3 ]. (ac.ir)
During ischemia and reoxygenation of the myocardium, the levels of free radicals increase in cardiomyocytes and Ca2+ transport as well as contractility are impaired due to oxidative damage to the ion-transporting protein complexes. (cardioweb.ru)

Ma S, Ma J, Tu Q, Zheng C, Chen Q, Lv W ( 2020 ) Isoproterenol Increases Left Atrial Fibrosis and Susceptibility to Atrial Fibrillation by Inducing Atrial Ischemic Infarction in Rats. (multichannelsystems.com)
Numerous apoptotic cardiomyocytes were found in ischemic fields in ischemia-reperfusion groups and werent observed in the sham-operated group. (ac.ir)
Molecular mechanisms of regulation of cardiomyocyte contractility during ischemic conditions and oxidative stress. (cardioweb.ru)

Mechanisms by which Dox promotes cardiac damage and cardiomyocyte cell death have been investigated extensively, but a definitive picture has yet to emerge. (oncotarget.com)

Both anatomical abnormalities, including atrial dilatation and abnormal ventricular structures, and functional deficits such as reduced blood flow and reduced strength of contractions were observed. (e-enm.org)

Toxic effects of Dox include cardiomyocyte damage and apoptotic and necrotic cell death [ 5 ]. (oncotarget.com)

Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
The adult mammalian heart is non-regenerative owing to the post-mitotic nature of cardiomyocytes. (nature.com)

Present study was designed to establish a causal connection between changes in the cell-cell junction protein expression at the blood-testis barrier and alterations in the adult rat testis histology following an anti-androgen flutamide exposure. (biomedcentral.com)
Short-term treatment with flutamide applied to adult rats exerts its primary effect on the basal ES, coexisting junctional complexes and their constituent proteins Cx43 and ZO-1, without any apparent morphological alterations in the seminiferous epithelium. (biomedcentral.com)

We study the mechanisms of protection of cardiomyocytes under ischemia and oxidative stress. (cardioweb.ru)

Heart muscle cells die en masse after injury, yet the adult mammalian heart retains little capacity to regenerate them. (natureasia.com)

To study the structural basis of pathological remodelling and altered calcium channel functional states in the heart, we sought to re-purpose high-affinity ligands of the cardiac calcium channel, the ryanodine receptor (RyR2), into super-resolution imaging probes. (whiterose.ac.uk)
Sarcomere length transients were measured in adult primary mouse cardiomyocytes using Calcium and Contractility System (IonOptix LLC, Westwood, MA) with digital sarcomere geometry measurement. (syncrosome.com)
Short-term Celf1 induction in adult animals results in disrupted transverse tubule organization and calcium handling. (abt-888.net)

Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)

Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)

We believe that by understanding the mechanisms regulating the formation of the heart during fetal development we can then apply these principles to understand the causes of adult heart diseases such as heart attack and heart failure. (stanford.edu)

Widespread transduction of cardiac and skeletal muscle has been achieved in adult mouse by intravenous administration of rAAV6 vector supplemented with vascular endothelium growth factor (VEGF) (Gregorevic et al. (justia.com)
Whilst vector administration in these studies was conducted with pharmacological interventions or in neonatal animals, rAAV8 vectors appear more efficient at crossing the blood vessel barrier and transducing heart and skeletal muscle of adult mice and hamsters (Wang et al. (justia.com)
In healthy young adults, the skeletal muscle mass comprises approximately 60% of total body mass. (frontiersin.org)

However, a persistent mismatch between intrinsic compensatory capacity and increased workload will eventually induce further functional deterioration and alteration in ventricular geometry (ventricular remodeling). (centerforpediatricresearch.org)

To address these hypotheses, we examined contractility, Ca 2+ handling, and ROS levels in individual cardiomyocytes isolated from control hearts, diabetic OVE26 hearts, and diabetic hearts overexpressing antioxidant protein metallothionein (MT). Our data showed that diabetic myocytes exhibited significantly reduced peak shortening, prolonged duration of shortening/relengthening, and decreased maximal velocities of shortening/relengthening as well as slowed intracellular Ca 2+ decay compared with control myocytes. (diabetesjournals.org)

Development provides an outstanding opportunity to identify coordinated AS regulation critical for physiological transitions from embryonic to adult functions. (abt-888.net)
Previously we showed that genes that undergo AS regulation during heart development produce transitions from GSK1324726A embryonic to adult protein isoforms GSK1324726A largely without changes in overall transcript levels presenting a new paradigm for understanding developmentally regulated gene expression in heart3. (abt-888.net)
The results revealed that most gene GSK1324726A expression and AS changes occurs within the first four weeks after birth and that CM and CF exhibit reciprocal transitions in expression of specific functional categories (proliferation cell adhesion cytokines-chemotaxis metabolism transcription regulation). (abt-888.net)
o the role of post-translational modifications of MLCK210, such as phosphorylation, acetylation, glutathionylation, as well as disease-associated genetic polymorphisms of MLCK210, in the regulation of its functional properties, is being studied. (cardioweb.ru)
Functional states of the sodium channel (closed, open, and inactivated) and their structure help to understand the cardiac regulation processes. (bvsalud.org)

In agreement with this, mice with ablation of N-cadherin in adult hearts via a cardiac-specific tamoxifen-inducible Cre N-cadherin transgene showed disrupted assembly of intercalated discs, dilated cardiomyopathy, impaired cardiac function, decreased sarcomere length, increased Z-line thickness, decreases in connexin 43, and a loss in muscular tension. (wikipedia.org)
Figure 3: Agrin induces cardiac regeneration in adult mice. (nature.com)

Pretreating the rats with simvastatin 18 hour prior to the induction of ischemiareperfusion has been shown to reduce cardiac dysfunction and improve coronary flow [ 7 ]. (ac.ir)

Imperacalcin (IpCa), a scorpion toxin peptide which induces channel sub-conduction states, and DPc10, a synthetic peptide corresponding to a sequence of the RyR2, which replicates arrhythmogenic CPVT functional changes, were used in fluorescent imaging experiments. (whiterose.ac.uk)
The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)

The heart of Sprague-Dowley rats was can-nulated at aortic level, attached to decellularization devices - Langendorff-Radnoti and simplified Lan-gendorff in alternative electric field, and perfused with SDS/EDTA solutions in different concentrations. (romanianjournalcardiology.ro)
Simplified Langendorff system in alternative electric field was more effective in decellularization of rat heart (5.5 hours), compared to classic system (12 hours), and the process was progressive, from the RV, to atria and LV. (romanianjournalcardiology.ro)
Our study showed that decellularization of the rat heart using the simplified Langendorff in alternative electric field is more efficient, because it used smaller amount of potentially noxious substances for the cardiac matrix. (romanianjournalcardiology.ro)
Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
Collaboration with VASA Therapeutics : characterization a heart failure model with preserved ejection fraction (HFpEF) / Dahl salt sensitive rats. (syncrosome.com)
The first four postnatal weeks involve a period of extensive physiological remodeling with dynamic changes as the fetal heart adapts to birth and converts to adult function. (abt-888.net)
Studies differ regarding adult mouse heart composition. (abt-888.net)
Heart relaxation also stands out as an active process, dependent on the energetic output and on specific ion and enzymatic actions, with the role of sodium channel being outstanding in the functional process. (bvsalud.org)

Adult hearts have little ability to regenerate [ 3 ] and traditional treatments fail to address the fundamental problem of muscle deficiency. (biomedcentral.com)

Strikingly diverse functional abnormalities have been identified for disease-linked ATP1A2 mutations which frequently lead to changes in the enzyme's voltage-dependent properties, kinetics, or apparent cation affinities, but some mutations are truly deleterious for enzyme function and thus cause full haploinsufficiency. (frontiersin.org)
This newly identified syndrome impacts both quality and quantity of life for both men and women, often leading to physical disabilities, gait abnormalities, and falls that cause loss of functional independence ( 4 ). (frontiersin.org)

Animals injected with cell-free solution (control group) demonstrated significant left ventricular (LV) functional deterioration, whereas grafting of iPSC-CMs attenuated this remodeling process. (biomedcentral.com)

However, when the production of ROS becomes excessive, oxidative stress will develop and impose a harmful effect on the functional integrity of biological tissue. (diabetesjournals.org)
The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (biomedcentral.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)

Cardiomyocytes require large numbers of healthy-functioning mitochondria to ensure sufficient ATP production for contractile function and cell survival. (oncotarget.com)

This in turn leads to a reduction in the exchange of respiratory gasses and nutrients between working cardiomyocytes and blood which results in reduced energy levels in these cells. (europeanpharmaceuticalreview.com)
Taken together, these and other data allowed us to propose a model of the functional organization of MLCK210 as the cytoskeleton integrator and motility organizer in non-muscle cells. (cardioweb.ru)
Seminiferous epithelium of both groups of rats displayed normal histology without any loss of germ cells. (biomedcentral.com)
As shown by examination of semi-thin and ultrathin sections, cell surface occupied by the basal ES connecting neighboring Sertoli cells and the number of gap and tight junctions coexisting with the basal ES were apparently reduced in flutamide-treated rats. (biomedcentral.com)
In the interstitial compartment, however, short-term exposure leads to both histological and functional changes of the Leydig cells. (biomedcentral.com)

In cardiac muscle, Cadherin-2 is found at intercalated disc structures which provide end-on cell-cell connections that facilitate mechanical and electrical coupling between adjacent cardiomyocytes. (wikipedia.org)
In many cases this approach has not permitted study of cell growth specifically at the cardiomyocyte level. (europeanpharmaceuticalreview.com)

Figure 2: Agrin delays neonatal cardiomyocyte maturation and is required for P1 cardiac regeneration following surgical resection. (nature.com)

Rats were deeply anesthetized with isoflurane (induction 4%, maintenance 2-2.5%) associated with buprenorphine (0.03mg/kg, SC) administrated 30 min before induction. (syncrosome.com)

This cardiomyopathy may be due to either organ-based damage, such as fibrosis, or to direct damage to cardiomyocytes. (diabetesjournals.org)

Energy starvation in hypertrophied cardiomyocytes is believed to occur as a result of a combination of factors, such as the reduction in the ratio in the surface area between capillaries and cardiomyocytes and increased myocyte diameter, see Figure 1 3 . (europeanpharmaceuticalreview.com)

To detect the expression of Fas in the cardiomyocytes, immunohistochemistry method was used. (ac.ir)
Vesicular trafficking genes affected by AS during normal development (when Celf1 is down-regulated) show a reversion to neonatal splicing patterns after Celf1 re-expression in adults. (abt-888.net)

which encodes the isoform 2 of the human Na + ,K + -ATPase's large catalytic α-subunit, which in the adult central nervous system (CNS) is mainly expressed in astrocytes. (frontiersin.org)
Find SPARC Antibodies for a variety of species such as anti-Human SPARC, anti-Mouse SPARC, anti-Rat SPARC. (antibodies-online.com)

Here, we summarize structural and functional data about the Na + ,K + -ATPase available to date and an overview is provided about the particular properties of the α 2 isoform that explain its physiological relevance in electrically excitable tissues. (frontiersin.org)
MMP2 and 9 KO and prototype pharmacological inhibitors protect from structural changes and functional deficit in animal HF models. (syncrosome.com)

This leads to a secondary functional deficiency of a number of ETF-dependent dehydrogenases and results in a combined disorder of both fatty acid oxidation and amino acid metabolism (especially lysine, tryptophan, and branched-chain amino acids). (nature.com)

Rats were prepared using standard surgical conditions and then intubated and ventilated. (syncrosome.com)

In addition, high glucose and angiotensin II promoted significantly increased generation of ROS in diabetic cardiomyocytes. (diabetesjournals.org)

Different studies have shown a different prognosis in T2DM patients and T2DM elderly patients treated by CRT-D. We reviewed the literature data on CRT-D effect on adult and elderly patients with T2DM as compared with nondiabetic patients. (hindawi.com)
Therefore, we reviewed the literature data on CRT effect on adult and elderly patients with T2DM as compared with nondiabetic patients. (hindawi.com)

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