Function stability inhibitor protein. Medical search. Frequent questions

Recent evidence indicates that altered degradation rate of mutant proteins represents a pathogenic mechanism underlying genetic diseases. (frontiersin.org)
The TCDD-induced degradation of AhR is inhibited by MG132, a potent inhibitor of the 26S proteasome, indicating the ubiquitin-26S proteasome mediated proteolysis as a mechanism for the degradation of AhR. (cdc.gov)
Furthermore, inhibition of protein synthesis by cycloheximide blocks the degradation of AhR by TCDD, suggesting a labile factor in controlling the stability of ligand-activated AhR (hence, designated as AhR degradation promoting factor, or ADPF). (cdc.gov)
These findings provide new insights into the mechanism by which TCDD-activated AhR is regulated in nucleus through the 26S proteasome protein degradation pathway. (cdc.gov)
Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)
Inhibiting ErbB2 leads to the inhibition of HSF1 transcriptional function, decline in HSP90 level, and subsequent degradation of mutp53 and MDM2 [5]. (silexon.tech)
Finding that endogenous Txnip protein is also rapidly degraded at the onset of adipogenesis suggested that Txnip degradation is required for adipocyte differentiation. (harvard.edu)
Targeted protein degradation as a therapeutic modality has experienced an explosion in research the past several years resulting in advancements of many degraders to the clinic as well as opening possibilities in terms of targets and disease indications. (fimecs.com)
Enable sensitive quantitation of PROTAC-mediated degradation of targets using Enzyme Fragment Complementation (EFC)-based cellular biosensor cell lines which combine EFC detection technology with CRISPR genome editing in our SPRINTer™ Protein Turnover Biosensor Assays. (fimecs.com)
The proteostasis network (PN) is an assembly of distinct dynamic molecular pathways that control the functionality of the proteome (proteome homeodynamics) during protein synthesis, folding, trafficking, and degradation. (hindawi.com)
Inhibition of VCP either by genetic depletion or the pharmacologic inhibitor CB-5083 increased ubiquitination and degradation of p53-R273H, leading to cell death. (aacrjournals.org)
USP7 as a deubiquitinase sustained PLK1 protein stability via the C223 site, and inversely, USP7 inhibition by P5091 promoted the protein degradation of PLK1 through the ubiquitination-proteasome pathway. (biomedcentral.com)
USP7 inhibition induces cell apoptosis and cell cycle G2/M arrest, and overcomes taxane resistance by inducing the protein degradation of PLK1, resulting in chromosome misalignment in mitosis. (biomedcentral.com)
In addition to the autophagy-lysosome system, the ubiquitin-proteasome system (UPS) takes up approximately 80 to 90% of intracellular protein degradation [ 1 ]. (biomedcentral.com)
In UPS-induced protein degradation, ubiquitin binds to target proteins and catalyzes them by a hierarchical cascade comprising E1, E2 and E3 ubiquitin ligases [ 2 ]. (biomedcentral.com)

When brassinosteroid binds to BRI1, it reduces the stability of the BRI1:BKI1 complex and promotes the binding of BRI1 to another membrane protein, BRI1-associated receptor kinase 1 (BAK1). (wikipedia.org)
It binds unstable SIRT1 and stabilizes it in an open, partially unfolded conformation, facilitating its activation and cellular functions. (silexon.tech)
PAI-1 mRNA-binding protein specifically binds the mRNA of type-1 plasminogen activator inhibitor (PAI-1), and is thought to be involved in regulation of mRNA stability [2]. (gzsys.org.cn)

TANK is an inhibitor of TRAF function which regulates TRAF protein activity via sequestering TRAFs in a dormant position in the cytoplasm. (prospecbio.com)
HSP90AA1 is involved in the assembly of the synaptic LRP1-HSP90AA1-PSD-95 complex, which regulates the stability and function of cerebellar synapses [7]. (silexon.tech)
Thioredoxin regulates adipogenesis through thioredoxin-interacting protein (Txnip) protein stability. (harvard.edu)
Txnip inhibits adipogenesis directly, and thioredoxin binding regulates Txnip by enhancing Txnip protein stability. (harvard.edu)
These studies present a novel reconsideration of Txnip-thioredoxin signaling by showing that thioredoxin regulates the intrinsic function of Txnip as an inhibitor of adipogenesis through protein stabilization. (harvard.edu)
It regulates the transcription of the cI protein and the Cro protein. (wikidoc.org)

Here, we describe zebrafish that are mutant in the serine protease inhibitor Hai1a (Spint1la),which display disrupted epidermal integrity. (biologists.com)
The SERPINA1 gene provides instructions for making a protein called alpha-1 antitrypsin, which is a type of serine protease inhibitor (serpin). (medlineplus.gov)

Kinases p38 and ERK1/2, that was abolished in the current BMS-794833 presence of the pharmacological inhibitors SB203580 and PD98059. (buyresearchchemicalss.net)
Isoform-specific pharmacological inhibitors of class I PI3K activity have undergone clinical development as anticancer therapeutics and for the treatment of human disorders caused by PI3K pathway hyperactivation. (nature.com)
The lack of structural information on PI3KC2α and related class II PI3Ks has also greatly hampered the development of isoform-selective pharmacological inhibitors for clinical applications. (nature.com)

Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. (neobiotechnologies.com)
Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. (nih.gov)
A structure-activity relationship study of 38 compounds in this structural family yielded highly potent (IC(50) 1M) Eis inhibitors, which did not inhibit other acetyltransferases. (cdc.gov)
We discovered that an antipsychotic drug, haloperidol (HPD, 1), was a potent Eis inhibitor with IC(50) = 0.39 0.08 M. We determined the crystal structure of the Eis-haloperidol (1) complex, which guided synthesis of 34 analogues. (cdc.gov)
While direct repurposing of haloperidol as an anti-TB agent is unlikely due to its neurotoxicity, this study reveals potential approaches to modifying this chemical scaffold to minimize toxicity and improve metabolic stability, while preserving potent Eis inhibition. (cdc.gov)

The intracellular domain of BRI1 functions as a kinase and it is this domain triggers the phosphorylation cascade that results in changes of gene expression. (wikipedia.org)
The transient receptor potential melastatin 4 (TRPM4) protein is an intracellular Ca 2+ -activated non-selective cation channel, which is impermeable to Ca 2+ . (frontiersin.org)
Molecular characterization of a novel intracellular hyaluronan-binding protein. (gzsys.org.cn)
Overexpression of the enhanced intracellular survival (Eis) protein by Mtb confers resistance to the second-line antibiotic kanamycin (KAN). Eis is an acetyltransferase that acetylates KAN, inactivating its antimicrobial function. (cdc.gov)

In vitro production of enzymatically active O. volvulus prolyl 4-hydroxylase should facilitate identification of specific inhibitors of the parasite enzyme. (embl.de)
Our results advance our understanding of PI3K biology and pave the way for the development of specific inhibitors of class II PI3K function with wide applications in biomedicine. (nature.com)

In the model plant species Arabidopsis thaliana, BRI1 acts alongside two homologous proteins, known as BRI1-LIKE1 (BRL1) and BRL3. (wikipedia.org)
These two homologous proteins directly bind p53 through their N-terminal domains and inhibit p53 transcriptional activity. (oncotarget.com)

If certain targets are identified, immune checkpoint inhibitors can be used. (karger.com)
Moreover, PD-1 and PD-L1 inhibitors are being tested in combination with other checkpoint inhibitors, targeted therapies, cancer vaccines, monoclonal antibodies, and other modalities. (shu.edu)

I contributed to the development of a general strategy of designing competitive inhibitors targeting RBPs. (ku.edu)

Also, execution of macrophage apoptosis by this proteins was seen to become mediated by toll like receptor 2 (TLR2) reliant discharge of TNF, and deletions inside the PGRS area attenuated its TNF-inducing capability. (buyresearchchemicalss.net)
The available data suggest that USP7 inhibitors induce cell cycle arrest and apoptosis in cancer cells through the p53 pathway, and sensitize cancer cells to PARP inhibitor-induced cell death [ 18 ]. (biomedcentral.com)
X-linked inhibitor of apoptosis protein (XIAP) predicts disease-free survival in BRAFV600E mutant papillary thyroid carcinoma in middle eastern patients. (nih.gov)

MG132, a proteasomal inhibitor, rescued PC-1 knockdown-dependent declines in α1C protein . (bvsalud.org)
Additionally, MG132 experiments indicate that other E3-ligases regulate p53 stability. (oncotarget.com)

One of these proteins is the fibroblast activation protein (FAP), a type II transmembrane serine protease with both dipeptidyl peptidase activity and endopeptidase activity ( 2 - 4 ). (snmjournals.org)

Therefore, the altered capacity of cancer cells to repair and/or replicate DNA is the basis of many classical therapies, such as platinum-based agents, and also recently introduced or upcoming therapies, such as PARP inhibitors or ATR inhibitors (reviewed in refs. (nature.com)

Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. (neobiotechnologies.com)
Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. (neobiotechnologies.com)
The accumulation of both Cyclin and CKI proteins is tightly regulated at the level of transcription. (intechopen.com)
In addition, Cyclin and CKI proteins are controlled at the level of their destruction. (intechopen.com)
p27 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclin E-CDK2, which phosphorylates pRb, thereby ushering the cell from G1 into S phase through the Restriction point (Figure 2). (shu.edu)
P15 INK4 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclind-CDK4,6, inhibiting it from hypophosphorylating Rb, thereby, rendering the cell cycle unresponsive to external proliferation signals. (shu.edu)
The cyclin D1-Cdk4 complex phosphorylates the pRB protein leading to sequential phosphorylation by cyclin E-Cdk2 and release of free E2F. (shu.edu)

Rämisch S, Weiniger U, Martinsson J, Akke M and Andre I "Computational design of Leucine-Rich Repeat proteins with a defined geometry" Proc Natl Acad Sci, 2014, pii: 201413638. (lu.se)

Fragile X syndrome (FXS) is caused by loss of the FMR1 gene product FMRP (fragile X mental retardation protein), a repressor of mRNA translation. (jneurosci.org)
Our data suggest that elevated protein synthesis in the Fmr1 KO is due to saturation of mRNA translation downstream of the MAP kinase ERK1/2 which is basally activated by mGluR5. (jneurosci.org)
This family includes the HABP4 family of hyaluronan-binding proteins, and the PAI-1 mRNA-binding protein, PAI-RBP1. (gzsys.org.cn)
Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the Type-1 plasminogen activator inhibitor mRNA. (gzsys.org.cn)
By using a mRNA having 5'cap and a poly(A)-tail with this extract in combination with a proprietary liposome, the translation reaction in vitro yields ample quantity of membrane protein which is captured by the liposome leading to correct conformation and folding essential for biological function. (abnova.com)

Thioredoxin overexpression stabilized Txnip protein levels to inhibit adipogenesis, and adipogenic stimulants such as insulin promoted Txnip-thioredoxin dissociation to the more labile free Txnip state. (harvard.edu)
Moreover, HGF was shown to inhibit amiloride-sensitive epithelia Na+ channel (ENaC) function in CF airway epithelium (Shen, Widdicomb, and Mrsny 1999), suggesting that its administration could also be useful to lessen the abnormally higher activity of ENaC observed in CF airway cells. (flapinhibitor.com)
The inhibitory SMADs, SMAD6 and SMAD7, constitute the third class, which function to inhibit TGF-β signaling (Figure 3). (shu.edu)

This dynamic relationship between the two proteins thus results in a feedback loop in which DNA damage-activated p53 promotes Mdm2 transcription while the translated Mdm2 protein inhibits p53 functions. (oncotarget.com)
This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. (nih.gov)

A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
Repeat proteins feature prominently in biology and serves as a diverse scaffold for protein-protein interactions. (lu.se)
One class of repeat proteins, Leucine Rich Repeats (LRRs), is widely used in nature. (lu.se)

After several decades of trying to address these challenges, researchers have amassed a considerable molecular tool box of tags and fusion proteins to aid in the expression and purification of recombinant proteins. (addgene.org)
What are some of the hurdles to overcome in order to overexpress a recombinant protein? (addgene.org)
Though there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione, can also protect against proteolysis. (addgene.org)

PC-1 is a cardiomyocyte mechanosensor that is required for cardiac hypertrophy through a mechanism that involves stabilization of α1C protein . (bvsalud.org)
Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can maltose-binding protein (MBP). (addgene.org)

HSP90 is also important for the stability and function of the BRAFV600E-mutant protein, and HSP90 inhibitors can overcome acquired resistance to BRAF inhibitors [2]. (silexon.tech)
HSP90AA1 inhibition enhances the effectiveness of MAPK inhibitors and prevents adaptive drug resistance in KRAS-mutant lung adenocarcinoma [10]. (silexon.tech)
HSP90/AXL/eIF4E-regulated unfolded protein response (UPR) is involved in adaptive resistance to certain drugs and intratumor heterogeneity in KRAS-mutant lung tumor cells [10]. (silexon.tech)
We previously found that the stabilizing double mutant L49I/I57V in the small protein chymotrypsin inhibitor 2 from barley led to distributed increased nanosecond and faster dynamics. (lu.se)

The and gene pairs had been observed to become co-operonic in exploits the PE protein to evade BMS-794833 the web host immune system response by changing the Th1 and Th2 type stability thus favouring bacillary success. (buyresearchchemicalss.net)
For instance, cI refers to the gene, while cI is the resulting protein encoded by that gene. (wikidoc.org)
In the absence of cI proteins, the cro gene may be transcribed. (wikidoc.org)
In the presence of cI proteins, only the cI gene may be transcribed. (wikidoc.org)
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. (nih.gov)
At a minimum, such profiling should check for the expression of HER-2 (a gene that plays a role in the development of multiple cancers, including breast cancer), Epstein-Barr virus, and programmed death ligand-1 (a molecule with immunoregulatory functions). (karger.com)
Many SERPINA1 gene variants change single protein building blocks (amino acids) in alpha-1 antitrypsin, which can alter the protein's structure. (medlineplus.gov)
Still other gene variants prevent the production of any alpha-1 antitrypsin protein. (medlineplus.gov)
RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
[ 3 ] Loss of function mutations in this gene are implicated in pediatric retinoblastoma. (medscape.com)
In addition we will address important areas of modern epidemiology such as the influence of early life factors on adult health and disease, but also the importance of the gene-diet-microbiota interaction for body function and health. (lu.se)

According to the metabotropic glutamate receptor (mGluR) theory of FXS, excessive protein synthesis downstream of mGluR5 activation causes the synaptic pathophysiology that underlies multiple aspects of FXS. (jneurosci.org)
Here, we use an in vitro assay of protein synthesis in the hippocampus of male Fmr1 knock-out (KO) mice to explore the molecular mechanisms involved in this core biochemical phenotype under conditions where aberrant synaptic physiology has been observed. (jneurosci.org)
We find that elevated basal protein synthesis in Fmr1 KO mice is selectively reduced to wild-type levels by acute inhibition of mGluR5 or ERK1/2, but not by inhibition of mTOR (mammalian target of rapamycin). (jneurosci.org)
These results suggest that the ERK1/2 pathway, and other neurotransmitter systems that stimulate protein synthesis via ERK1/2, represent additional therapeutic targets for FXS. (jneurosci.org)
Although it is now clear that mGluR5 participates in the pathogenesis of FXS, at least in animal models, it is still poorly understood how Gp1 mGluRs trigger protein synthesis and how this process is altered in the absence of FMRP to disrupt synaptic function. (jneurosci.org)
One source of confusion may be that proxy measures of protein synthesis, such as mGluR-LTD or phosphorylation of signaling molecules, have been used in intact hippocampal slice preparations, whereas metabolic labeling experiments have been performed in synaptoneurosome preparations of cortex that are not easily related to altered hippocampal synaptic plasticity. (jneurosci.org)
A strong rationale for taking this approach is that slice has been shown to accurately reproduce the in vivo phenotype of elevated basal protein synthesis in the Fmr1 KO hippocampus (cf. (jneurosci.org)
Wheat embryos store all of the components of translation in a concentrated dried state, ready for protein synthesis as soon as germination starts. (abnova.com)

Due to the wide range of binding targets, their large interaction surfaces and biophysical properties, LRR proteins have been suggested as alternatives to antibodies as scaffolds for protein binding. (lu.se)

Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. (lookformedical.com)
Factors to consider when assessing the level of immune competence in a patient include underlying disease severity, duration, clinical stability, complications, comorbidities, and any potentially immune-suppressing treatment. (cdc.gov)

As an α-arrestin protein, Txnip has two C-terminal tail PPXY motifs that mediate E3 ubiquitin ligase binding and Txnip protein stability. (harvard.edu)
However, in both cases, the sequence motifs predicted to be important for ligand binding are not conserved throughout the family, so it is not known whether members of this family share a common function. (gzsys.org.cn)
The coiled coil is one of most recurring structural motifs in eucarytotic proteins, found in for example transcription factors, motor and skeletal proteins. (lu.se)

From the 69 PPE proteins, 22 can be found in forecasted operonic clusters solely formulated with PE/PPE genes [2], [4]. (buyresearchchemicalss.net)
In the following paragraphs, we will write genes in italics and their associated proteins in Roman. (wikidoc.org)
p53 affects the transcription of many target genes and interacts with key cellular proteins. (molvis.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Driver events in pediatric malignancies can occur through loss of function in tumor suppressor genes or gain of function in proto-oncogenes. (medscape.com)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)

3) HSP90 interacts with DYRK3 to regulate its stability and activity. (silexon.tech)
It interacts with co-chaperones and client proteins to regulate their stability, function, and activation. (silexon.tech)
USP7 interacts with PLK1 protein through its PBD domain by catalytic activity. (biomedcentral.com)

In the research literature, the protein phosphatase inhibitor okadaic acid is described as an inducer of ARNT homodimer formation. (uni-goettingen.de)
These events resulted in upregulation of the Cdk4/6 inhibitor p15 INK4B and repression of the Cdk-activating phosphatase Cdc25A. (shu.edu)

Here, we analyzed the biochemical pathway and function of the downregulation. (cdc.gov)
Revealing how molecular interactions are optimized to control both oligomerization state and assembly pathway in protein assemblies is of vital importance for our understanding of biomedically important biological assemblies, as well as for efforts to manipulate them. (lu.se)

In addition to these catalytic roles, PI3KC2α is also required for genome stability by acting as a scaffold at the mitotic spindle during cell division 26 . (nature.com)
This review discusses the function of sEH in mammals and its protein structure and catalytic activities. (frontiersin.org)
This review aims to summarize the role of sEH in mammals and its protein structure and catalytic activities. (frontiersin.org)
The first project, focused on activation of protein function, is built on an approach developed by our laboratory that designs a de novo allosteric binding site directly into the catalytic domain of an enzyme. (ku.edu)

Mitochondria are highly dynamic organelles that provide essential metabolic functions and represent the major bioenergetic hub of eukaryotic cell. (hindawi.com)
Prokaryotes can also have a hard time folding eukaryotic proteins. (addgene.org)
Abnova in vitro protein expression system is based on the eukaryotic translational apparatus of wheat germ. (abnova.com)

Fibroblast activation protein (FAP) is overexpressed in cancer-associated fibroblasts and is involved in a variety of tumor-promoting activities such as matrix remodeling, angiogenesis, chemotherapy resistance, and immunosuppression. (snmjournals.org)
An anticancer drug is coupled to an antibody that targets a specific tumor antigen (or protein ) that, ideally, is only found in or on tumor cells. (wikipedia.org)
These are drugs that block the protein-protein binding that prevents T cells from killing tumor cells. (karger.com)
Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. (lookformedical.com)
Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. (lookformedical.com)
We will discuss three key proteins that function as tumor suppressors implicated in the development of pediatric (and some adult) cancers: pRB, p53, and PTEN. (medscape.com)
The two-hit hypothesis has now been adopted as a key mechanism for the loss of function of tumor suppressors leading to oncogenesis. (medscape.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)

It has been shown that IL-31 can induce the secretion of monocyte chemotactic protein-1 (MCP-1). (faahinhibitor.com)

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)
pRB functions as a negative regulatory transcription factor during the G1 to S phase cell cycle transition. (medscape.com)

We hypothesize that the interactions between the substituted residues and the well-ordered water molecules links subtle structural changes around the substituted residues to the region in the protein that experience slow conformational changes. (lu.se)
Proline is often a rigid residue (Figure 5(a)), in addition to a transform to leucine would boost the flexibility from the protein structure in this location (possibly affecting the turn conformation) also as supply a prospective to make van der Waals interactions. (faahinhibitor.com)
The atomic interactions between subunits in protein assemblies are finely tuned to control affinity as well as quaternary structure. (lu.se)
Large protein assemblies are typically held together by weak interactions between individual components but the multiplicity of interactions among the large number of subunits can lead to exquisite specificity. (lu.se)

The SMAD proteins are a family of transcription factors consisting of 8 members, SMAD1-8, which are further subdivided into 3 classes based on structural and functional properties. (shu.edu)

Recently small molecular inhibitors have been developed to target USP7. (biomedcentral.com)
Biochemists and molecular biologists who need to overexpress and purify proteins can face any number of technical challenges depending on their protein of interest. (addgene.org)
An affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein. (addgene.org)
Selective activation or inhibition of a particular protein function can help elucidate crucial molecular mechanisms and enables important advances in cell biology. (ku.edu)
Our goal is to achieve molecular level control of protein self-assembly. (lu.se)

In consequence, protein turnover of WT-TRPM4 and TRPM4 variants overexpressed in HEK293 cells was analyzed using cycloheximide, an inhibitor of protein biosynthesis. (frontiersin.org)
Adult and embryonic Brugia malayi are shown to be susceptible to inhibitors of vertebrate prolyl 4-hydroxylase, with exposed parasites exhibiting pathologies consistent with a disruption in cuticle biosynthesis. (embl.de)

BRI1 is an integral membrane protein. (wikipedia.org)
We will use our robust in vitro wheat germ expression system combined with proprietary liposome technology to produce your membrane protein of interest. (abnova.com)
The primary defect in Dubin-Johnson syndrome is a mutation in an apical canalicular membrane protein responsible for the excretion of bilirubin and other nonbile salt organic anions. (medscape.com)

Several chemoinformatics approaches were used in this study, reporting four multi-target inhibitors: MCULE-7149246649-0-1, MCULE-6730554226-0-4, MCULE-1176268617-0-6 and MCULE-8592892575-0-1 with high binding energies that indicate better AChE inhibitory activity. (unboundmedicine.com)
The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)

To this end I explored two parallel paths, one to study activation and one to study inhibition of protein functions. (ku.edu)
In parallel to these efforts I carried out studies aimed at inhibition of protein function, as exemplified by my project that uses small molecules to disrupt a protein-RNA interaction. (ku.edu)

Characterization of the human ortholog of Mov34 reveals eight N-terminal residues important for MPN domain stability. (nih.gov)

However, the anticancer mechanism of USP7 inhibitors is still elusive. (biomedcentral.com)
When tested in Mtb cultures, two inhibitors (47 and 55) completely abolished resistance to KAN of the highly KAN-resistant strain Mtb mc(2) 6230 K204, likely due to Eis inhibition as a major mechanism. (cdc.gov)
Their loss of function is a well described mechanism of malignant proliferation. (medscape.com)

It is highly specific and shows no cross-reaction with other related mitotic inhibitors. (neobiotechnologies.com)
Moreover, we describe a PI3KC2α-specific helical bundle domain that underlies its scaffolding function at the mitotic spindle. (nature.com)

Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. (lookformedical.com)
Abnormal alpha-1 antitrypsin proteins, including those produced from the Z allele, may bind together to form a large molecule, or polymer, that cannot leave the liver. (medlineplus.gov)
Alzheimer Disease Alzheimer disease is a progressive loss of mental function, characterized by degeneration of brain tissue, including loss of nerve cells, the accumulation of an abnormal protein called beta-amyloid. (msdmanuals.com)

Chronic kidney disease is marked by progressive fibrosis and consecutive loss of function - the availability of specific therapies is limited. (uni-goettingen.de)

BRI1 belongs to the large leucine-rich receptor-like protein kinase family. (wikipedia.org)
B-Raf is a member of the Raf kinase family of growth signal transduction protein kinases. (rafinhibitors.com)

To further determine whether BCR ABL phosphorylates Raf Inhibitors hTERT, we treated K562 cells with 1 M Gleevec, and evaluated the phosphorylation status of hTERT. (rafinhibitors.com)

Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)

A fourth BRI1-family protein, BRL2 cannot bind brassinosteroid and its function is unknown. (wikipedia.org)
Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)

Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)

Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)

Protein tags are usually smallish peptides incorporated into a translated protein. (addgene.org)
An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. (nih.gov)
We use structure based modeling and computational protein design methods to optimize the protein sequence of proteins and peptides in order to control their assembly. (lu.se)

The PE sub-group contain 34 proteins from the PE just course characterised by a comparatively conserved N-terminus of 110 aa, and 67 PE_PGRS (polymorphic GC wealthy recurring sequences) proteins using a C-terminal area made up of glycine-rich do it again regions which range from 100 to over 500 aa long. (buyresearchchemicalss.net)
It's also recommended to check for predisposition to mutation, which is assessed by looking at the stability of certain short, repeated sequences of DNA called microsatellites. (karger.com)

The initial PE/PPE multigene category of proteins occupies nearly 10% from the coding sequence of (pathogenesis, their precise physiological functions remain generally undefined. (buyresearchchemicalss.net)

Given that brain cholesterol disturbances play a role in the onset of Alzheimer's disease (AD) as well as of other neurodegenerative diseases, understanding the functions of this enzyme could provide pivotal information on the pathophysiology of these conditions. (frontiersin.org)

It encodes a large nuclear protein that is a component of DNA repair pathways. (lookformedical.com)
It encodes a large, nuclear protein that is an essential component of DNA repair pathways, suppressing the formation of gross chromosomal rearrangements. (lookformedical.com)
TP53 encodes the protein p53, which is known as the "guardian of the genome. (medscape.com)

This hypothesis ALK4 Inhibitor Storage & Stability requires to become further investigated. (faahinhibitor.com)

Slow conformational changes are often directly linked to protein function. (lu.se)
The main challenge of generalizing this approach, however, is the difficulty of rationally designing analogous conformational changes in other proteins. (ku.edu)
Through collaborative and multidisciplinary efforts, we find that the switchable proteins built via the chemical rescue of structure are frequently controlled indirectly by modulating protein stability, rather than discrete conformational changes. (ku.edu)
Since energetic evaluation of protein stability is far more tractable than designing and/or predicting allosteric conformational changes, this finding demonstrates how chemical rescue of structure can be applied to other systems for building a variety of new protein switches. (ku.edu)

Compared with the previously published ligand, FAPI-02, FAPI-04 showed excellent stability in human serum, higher affinity for FAP as opposed to CD26, and slower excretion in vitro. (snmjournals.org)
The specific PP2A inhibitor LB-100 induced the formation of ARNT homodimers in vitro and increased protein stability of Arnt. (uni-goettingen.de)
In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities. (medlineplus.gov)

Abnova has garnered a large collection of catalogue membrane proteins covering several essential protein families to facilitate drug discovery and validation research. (abnova.com)

Mutations that disrupt PTEN function define a condition called PTEN hamartoma syndrome, which also includes Cowden syndrome , Bannayan-Riley-Ruvalcaba syndrome, and Proteus/Proteus-like syndrome. (medscape.com)

It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- � � induced G1 arrest. (neobiotechnologies.com)

Overexpression can also lead to insolubility, and aggregated protein is not useful protein. (addgene.org)

The small protein ubiquitin plays a vital role in virtually all aspects of cellular life. (intechopen.com)

Inversely, the ubiquitination is removed from the labeled proteins or from polyubiquitin chains by deubiquitinating enzymes (or deubiquitinases, DUBs). (biomedcentral.com)

The mitochondria have their own chaperones and proteolytic enzymes that remove damaged or unfolded proteins [ 18 - 20 ]. (hindawi.com)
Conventional methods of inhibitor design mostly target RNA-processing enzymes and cannot be generalized to the majority of RNA-binding proteins (RBPs). (ku.edu)

This genoprotective function of p53 necessitates its level and activity be tightly regulated in cells under both homeostatic and post-DNA damage conditions. (oncotarget.com)

Editorial: Glial heterogeneity: impact on neuronal function and dysfunction. (unil.ch)
In these sufferers, LIF expression was identified in hippocampus and inside the temporal cortex, indicating a function for LIF in neuronal damage or repair in these web sites [21]. (faahinhibitor.com)

Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
Txnip (thioredoxin-interacting protein) is a critical mediator of metabolism and adipogenesis in vivo. (harvard.edu)
This system has significant advantages over commonly used protein expression platforms such as E. coli , insect cell, and mammalian cell which are all in vivo systems and under the constraint of cell membrane leading to low yield and stability. (abnova.com)

Development of Eis inhibitors as KAN adjuvant therapeutics is an attractive path to forestall and overcome KAN resistance. (cdc.gov)

Anatomy16

Organisms9

Diseases4

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes83

Disciplines and Occupations3

Technology, Industry, Agriculture2

Information Science5

Health Care3

Degradation15

Binds3

Regulates6

Protease inhibitor2

Pharmacological3

Potent6

Intracellular4

Specific inhibitors2

Homologous proteins2

Checkpoint inhibitors2

Competitive inhibitors1

Apoptosis3

MG1322

Serine1

PARP1

Cyclin7

Proc Natl Acad Sci1

MRNA5

Inhibit3

Inhibits2

Repeat proteins3

Recombinant3

Stabilization2

Mutant4

Gene11

Synthesis8

Antibodies1

Immune2

Motifs3

Genes6

Interacts3

Phosphatase2

Pathway2

Catalytic4

Eukaryotic3

Tumor8

Induce1

Regulatory2

Interactions4

Transcription1

Molecular5

Biosynthesis2

Membrane protein3

Inhibitory2

Inhibition of protein2

Characterization1

Mechanism3

Mitotic2

Abnormal3

Therapies1

Kinase family2

Phosphorylates1

Disulfide isomerase3

Bind2

Autophagy1

Fluorescent1

Peptides3

Sequences2

Coding sequence1

Enzyme1

Encodes3

Hypothesis1

Conformational changes4

Vitro3

Catalogue1

Disrupt1

Progression1

Overexpression1

Small protein1

Ubiquitination1

Enzymes2

Tightly regulated1

Neuronal2

Vivo3

Therapeutics1