Fulllength cdna. Medical search. Frequent questions

Scholars@Duke publication: Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (duke.edu)
To identify novel cytokine-related genes, we searched the set of 60,770 annotated RIKEN mouse cDNA clones (FANTOM2 clones), using keywords such as cytokine itself or cytokine names (such as interferon, interleukin, epidermal growth factor, fibroblast growth factor, and transforming growth factor). (nottingham.edu.cn)
In this study, we devised a program which enables us to correlate a large number of DNA accession numbers to the FANTOM (functional annotation of the mouse) full-length mouse cDNA clone set, and made a correlative table between mouse GeneChip clones and FANTOM clones. (elsevierpure.com)
Ajioka, I, Maeda, T & Nakajima, K 2006, ' Large-scale correlation of DNA accession numbers to the cDNAs in the FANTOM full-length mouse cDNA clone set ', Keio Journal of Medicine , vol. 55, no. 3, pp. 107-110. (elsevierpure.com)
To estimate the coverage of unique genes, we used FANTOM (Functional Annotation of RIKEN Mouse cDNA Clones) clusters (the RIKEN Genome Exploration Research Group 2001). (elsevierpure.com)
Functional annotation of a full-length mouse cDNA collection. (jax.org)

Could i please know, if there are any tools/scripts available which can run locally to predict full-length cDNA sequences. (biostars.org)
In contrast to the large number of ESTs available, including the ESTs from 23 developmental and tissue specific cDNA libraries contributed by the Salmon Genome Project (SGP), the number of sequences where the full-length of the cDNA insert has been determined has been small. (biomedcentral.com)
High quality full-length insert sequences from 560 pre-smolt white muscle tissue specific cDNAs were generated, accession numbers [GenBank: BT043497 - BT044056]. (biomedcentral.com)
By using AS Viewer, each AS event can be analyzed in the context of full-length cDNAs, enabling the user's empirical understanding of the relation between AS event and the consequent alternations in the encoded amino acid sequences together with various kinds of affected protein motifs. (edu.sa)
Full-length RNA or cDNA that includes capsid sequences derived from wild poliovirus, unless viruses derived from them are demonstrably proven to be safer than Sabin strains. (cdc.gov)
To aid in the examination of this phenomenon, the cDNA sequences encoding estrogen receptor-alpha (esr1), estrogen receptor-beta-a (esr2a) and ovarian aromatase (cyp19a1a) for the yellow perch were obtained. (usda.gov)
The following GMFG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
These sequences represent the protein coding region of the GMFG cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
In silico identification by nucleotide sequence alignment between genomic and cDNA sequences revealed three putative introns in GBSS I. The longest intron was 121 bp long and was located between nucleotide 595 and 716. (scialert.net)

First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. (duke.edu)
Construction of a full-length cDNA infectious clone of a European-like type 1 PRRSV isolated in the U.S. (nebraska.edu)
Construction of a full-length cDNA infectious clone of a European-like type 1 PRRSV isolated in the U.S. In The Nidoviruses: Toward Control of SARS and other Nidovirus Diseases (pp. 605-608). (nebraska.edu)
However, GeneChip only provides information on the identity of the molecules, and the investigator must obtain each cDNA clone for further analyses. (elsevierpure.com)
An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by in vitro transcription. (ias.ac.in)

ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. (duke.edu)
We describe a computer-based method that selects representative clones for full-length sequencing in a full-length cDNA project. (elsevierpure.com)
Clustering separates those cDNA clones into distinct clusters. (elsevierpure.com)
ACID is an online database for information about microarray cDNA clones. (lu.se)

The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. (sigmaaldrich.com)
The use of AMV RT for first-strand cDNA synthesis has certain advantages. (sigmaaldrich.com)
allows effective synthesis of complex RNA structures with full-length cDNA. (thomassci.com)
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc. (thomassci.com)
Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by Author Manuscript standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. (cdc.gov)
Widely used RNA-seq methods start with adapter ligation and cDNA synthesis of biological RNA samples followed by PCR amplification to generate sequencing libraries1. (cdc.gov)
Significant obstacles for the sequencing of tRNA include the presence of numerous post-transcriptional modifications and its stable and extensive secondary structure, which interfere with cDNA synthesis and adapter ligation. (cdc.gov)
Three specific modifications, N1-methyladenosine (m1A), N3-methylcytosine (m3C), and N1-methylguanosine (m1G), are abundant in eukaryotic tRNAs and are particularly problematic for reverse transcriptases (RTs) by causing cDNA synthesis to stop or misincorporate. (cdc.gov)

We have cloned and sequenced cDNA of trypsin gene from Aedes (Ochlerotatus) taeniorhynchus (Weidemann) adult. (usda.gov)
In this current paper, we have cloned and sequenced the full length cDNAs for ER? (usda.gov)
The full length cDNAs for TIF3 and TEF-1d were cloned and sequenced. (cdc.gov)

Full-length sequenced inserts from cDNAs (FLIcs) are an important tool for correct annotation and clustering of the genomic sequence in any species. (biomedcentral.com)
In this database, each of the alternative splicing (AS) variants corresponds to a completely sequenced and carefully annotated human full-length cDNA, one of those collected for the H-Invitational human-transcriptome annotation meeting. (edu.sa)
J:80000 The FANTOM Consortium and The RIKEN Genome Exploration Research Group Phase I & II Team, Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. (jax.org)

This suggests that the remaining cDNA libraries generated by SGP represent a valuable cCDS FLIc source. (biomedcentral.com)
Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes. (jax.org)

This paper describes the first Atlantic salmon FLIcs from a tissue and developmental stage specific cDNA library. (biomedcentral.com)
Zyagen offers the largest selection of ready to use full length tissue cDNA. (delos.info)

Grouping places together variant cDNAs, family genes, and cDNAs with sequencing errors. (elsevierpure.com)
In an effort to gain more understanding into the yellow perch E2 stimulated SSD, the cDNAs for genes that encode for hormones that are involved with the endocrine growth axis (GH, PRL, SL and IGF-I) have been recently cloned and characterized. (usda.gov)

Can i consider an sequence to be full-length cDNA, if the ORF in the sequence aligns with ~99%-100% identity, with 100% coverage to the reference protein? (biostars.org)
Here, we describe a method that uses PacBio long reads to sequence full-length cDNAs from individual transcriptomes and metatranscriptome samples. (pacb.com)
The sequence of the alpha 2 beta cDNA shows the presence of a single Arg residue between Gln 142 of the alpha 2 chain and Ileu 1 of the beta chain. (rti.org)

The cDNA is primed with oligo dT or random hexamer primers and is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, and gene cloning and target sequencing. (delos.info)
In vitro translation of human liver mRNA hybridizing with this cDNA gives a protein mol. (rti.org)

As quality control test, each cDNA sample is tested by PCR amplification of beta-actin. (delos.info)

RACE, rapid amplification of cDNA ends. (cdc.gov)

As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)

Complete sequencing and characterization of 21,243 full-length human cDNAs. (nih.gov)

15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. (nature.com)

Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)

1998). „Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII" . (wikipedia.org)

2004). „The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)" . (wikipedia.org)

Candida maltosa NADPH-cytochrome P450 reductase: cloning of a full-length cDNA, heterologous expression in Saccharomyces cerevisiae and function of the N-terminal region for membrane anchoring and proliferation of the endoplasmic reticulum. (mdc-berlin.de)

Overexpression of the cDNA-encoded TIF3 and TEF-1d proteins in NIH3T3 cells was oncogenic as evidenced by the appearance of transformed foci capable of anchorage-independent growth on soft agar and tumorigenesis in nude mouse. (cdc.gov)

High-efficiency full-length cDNA cloning. (jax.org)

However, these methods typically rely on sequencing short fragments of cDNA, then attempting to assemble the pieces into full-length transcripts. (pacb.com)

PURPOSE In an effort to provide the research community with high quality, full-length mammalian cDNA clones and sequences, the National Institutes of Health (NIH) has established the Full-Length cDNA Initiative, managed by a trans-NIH steering committee. (nih.gov)
Use of human tissues may be necessary to achieve the goal of a complete set of human cDNA clones. (nih.gov)
This RFA is intended to support innovative research projects aimed at solving one or more of the problems currently associated with the production of a complete set of full-length human cDNA clones and full-length cDNA clones from other mammals. (nih.gov)
RESEARCH OBJECTIVES Background: Access to high quality mammalian cDNA clones and sequences is critical for the rapid progression of biomedical research. (nih.gov)
The ultimate goal of this NIH-wide initiative is to generate a complete set of full-length human cDNA clones and their sequences as well as cDNA clones and sequences from other mammalian species. (nih.gov)
We obtained a total of 39,936 soybean cDNA clones (GMFL01 and GMFL02 clone sets) in a full-length-enriched cDNA library which was constructed from soybean plants that were grown under various developmental and environmental conditions. (nih.gov)
Our data indicated that 87.7% of the soybean cDNA clones contain complete coding sequences in addition to 5'- and 3'-untranslated regions. (nih.gov)
A large set of soybean full-length cDNA clones reported in this study will serve as a useful resource for gene discovery from soybean and will also aid a precise annotation of the soybean genome. (nih.gov)
The full-length enriched cDNA clones will enable the construction of overexpressing mutant plants by introduction of the cDNAs driven by a constitutive promoter, the complementation of Thellungiella mutants, and the determination of promoter regions in the Thellungiella genome. (nih.gov)
To evaluate PMTV genetic variation in Peru, we generated full-length cDNA clones, which allowed reliable comparative molecular and pathobiological characterization of individual isolates. (cipotato.org)
Using infectious cDNA clones of the representatives of these two clades, as well as reassortants for the RNA-CP genomic component, we determined the pathobiological differences between the lineages, which we coined as S (for severe) and M (for mild) types. (cipotato.org)
Using a panel of contiguous cDNAs that span the entire genome, we have assembled a full-length cDNA of the SARS-CoV Urbani strain, and have rescued molecularly cloned SARS viruses (infectious clone SARS-CoV) that contained the expected marker mutations inserted into the component clones. (nih.gov)
The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. (nih.gov)
Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. (nih.gov)
To search for markers we constructed cDNA libraries from human colon carcinoma cell lines and screened for clones that hybridize to a greater degree with mRNAs of colon carcinomas than with their normal counterparts. (nih.gov)
In this study, we constructed a full-length cDNA library from bacteria-immunized C. tripartitus and then selected up-regulated clones using differential screening by a dot blot hybridization analysis. (hindawi.com)
As an initial screening process, a total of 1862 cDNA clones were randomly selected from a full-length cDNA library, spotted onto Hybond-N membranes (Amersham Biosciences, Uppsala, Sweden) using a 96-well format dot blotter (Bio-RAD) and then hybridized with probes from bacteria-injected larvae as described previously [ 13 ]. (hindawi.com)
Coprisin cDNA clones were identified by a dot blot hybridization. (hindawi.com)
The cDNA library consists of 42,240 clones. (usda.gov)
We sequenced 196 randomly picked cDNA clones and compared their sequences with those of genes from other fungi in the GenBank databases. (usda.gov)
The full-length cDNA library consisted of 42,240 clones with an average cDNA insert of 1.9 kb. (usda.gov)
Of 196 randomly picked full-length cDNA clones that were sequenced, complete sequences were obtained for 149 clones and 47 clones had two partial sequences. (usda.gov)
All clones corresponding to the ESTs in the five full-length cDNA libraries are distributed from RIKEN Bio Resource Center (http://www.brc.riken.jp/lab/epd/Eng/index.shtml). (nibb.ac.jp)

Current methods of cDNA clone and library production favor shorter, more heavily represented genes. (nih.gov)

Full-length cDNA sequence and genomic or. (deu.edu.tr)
A full-length cDNA library with complete genomic coverage is a powerful tool for functional genomic studies. (usda.gov)

This invention relates to an infectious full length Langat virus cDNA which has been successfully constructed and can be used to further attenuate this naturally attenuated tick-borne flavivirus. (nih.gov)
A full-length cDNA of mandarinfish (Siniperca chuatsi) Mx gene was obtained using reverse transcription polymerase chain reaction (RT-PCR) and RACE (rapid amplification of cDNA ends) polymerase chain reaction (PCR) amplification of total RNA extracted from liver of a mandarinfish which was infected with Infectious spleen and kidney necrosis virus (ISKNV) (GenBank accession No.AY392097). (virosin.org)

To address current limitations, we developed a quantitative full-length direct cDNA sequencing strategy to simultaneously profile viral and host cell transcripts. (biorxiv.org)
WU Hai-feng, BAI Jun-jie, LAO Hai-hua, YE Xing, JIAN Qing, LUO Jian-ren.Cloning and Sequencing of the Full-length cDNA of Mx Protein from Mandarinfish .VIROLOGICA SINICA, 2004, 19(3): 271-275. (virosin.org)

Overexpression of SsDREB cDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. (hindawi.com)
Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)
Overexpression of the cDNA-encoded TIF3 and TEF-1d proteins in NIH3T3 cells was oncogenic as evidenced by the appearance of transformed foci capable of anchorage-independent growth on soft agar and tumorigenesis in nude mouse. (cdc.gov)

Information on functional domains and Gene Ontology (GO) terms for the RTFL cDNAs were obtained using InterPro. (nih.gov)
For ParDAM6 , only a small part of the cDNA was sequenced, which was sufficient for the quantification of gene expression. (frontiersin.org)

To study the functional genomics and identify genes involving in the biology of the pathogen, we constructed a full-length cDNA library with the spores of the wheat stripe rust pathogen. (usda.gov)
The results demonstrated the usefulness of the full-length cDNA library in identifying functional genes of the pathogen. (usda.gov)

Availability of a SARS-CoV full-length cDNA provides a template for manipulation of the viral genome, allowing for the rapid and rational development and testing of candidate vaccines and therapeutics against this important human pathogen. (nih.gov)

Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. (nih.gov)
11. XenDB: full length cDNA prediction and cross species mapping in Xenopus laevis. (nih.gov)

ZyagenZyagen offers the largest selection of ready to use full length tissue cDNA. (delos.info)

We have constructed a full-length cDNA library from urediniospores of race PST-78 of Puccinia striiformis f. sp. (usda.gov)

Expression of CoFad2 cDNA in yeast (Saccharomyces cerevisiae) indicated it encodes a Delta12 desaturase that introduces a double bond at the 12 position of 16:1(9Z) and 18:1(9Z). (researchgate.net)

RACE, rapid amplification of cDNA ends. (cdc.gov)
As quality control test, each cDNA sample is tested by PCR amplification of beta-actin. (delos.info)

This RFA is a one-time solicitation although it may be reissued if this area of research remains an identified area of high priority by the advisory groups to the NIH Full-Length cDNA Program. (nih.gov)
The PCR-ready to use first strand cDNA is synthesized from high. (delos.info)

Mint-2 cDNA synthesis kit is designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA. (evrogen.com)

The cDNA-derived ZIKV replicated efficiently in a variety of cell lines, including those of both neuronal and placental origin. (nih.gov)

Although a variety of cDNA development and production efforts have been and continue to be supported by the NIH, there is a need to expand upon and consolidate these existing efforts. (nih.gov)

This RFA, Technologies for Generation of Full-Length Mammalian cDNAs, is related to several priority areas, including cancer, heart disease and stroke, diabetes and chronic disability conditions and maternal and fetal health. (nih.gov)

This Request for Application (RFA) targets one component of the initiative: cDNA cloning technology development. (nih.gov)

Anatomy26

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