AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Molecular Sequence DataBase SequenceRNA, MessengerAmino Acid SequenceTranscription, GeneticCloning, MolecularDNA, ComplementaryAlternative SplicingDNALength of StayBlotting, NorthernSequence AlignmentSequence Homology, Amino AcidReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNARNAExonsGene ExpressionGene Expression ProfilingPolymerase Chain ReactionCell LineGene LibraryGene Expression RegulationSequence Homology, Nucleic AcidMutationDNA PrimersNucleic Acid HybridizationGenesProtein IsoformsRNA SplicingPhylogenyRestriction MappingPromoter Regions, GeneticIntronsProtein BiosynthesisOpen Reading FramesExpressed Sequence TagsPlasmidsEscherichia coliRNA, AntisenseTransfectionRecombinant ProteinsPolymorphism, Restriction Fragment LengthGene Expression Regulation, PlantBlotting, SouthernProtein Structure, TertiaryOligonucleotide Array Sequence AnalysisChromosome MappingTranscription FactorsPoly AProtein BindingIn Situ HybridizationDNA-Binding ProteinsRNA, ViralPlant ProteinsBinding SitesCells, CulturedMultigene FamilyGene Expression Regulation, DevelopmentalTime FactorsRecombinant Fusion ProteinsSpecies SpecificityKineticsNucleic Acid ConformationDNA Restriction EnzymesGenes, PlantOrgan SpecificityRNA Processing, Post-TranscriptionalTumor Cells, CulturedBlotting, WesternOligonucleotide ProbesHeLa CellsSequence DeletionRepetitive Sequences, Nucleic AcidConserved SequenceTissue DistributionRNA, Plant3' Untranslated RegionsPhenotypeCarrier ProteinsMembrane ProteinsRNA-Binding ProteinsRNA StabilityProteinsSaccharomyces cerevisiaeCell NucleusTranscriptomeGenetic VariationSequence Analysis, RNAModels, GeneticNuclear ProteinsBacterial ProteinsMolecular WeightModels, MolecularDNA, ViralArabidopsisElectrophoresis, Polyacrylamide GelChickensGene Expression Regulation, EnzymologicGenetic Vectors
VariantsIsoformsIsoformMRNA transcriptEpisode transcriptProteinGenomicCDNAGenesGenome annotationDifferentialYieldsAbundanceVariantAccumulationExperimentalSynthesisGene-levelPodcastTranscriptomeExtentClipsFunctionalResearchExpression of full-lengthSpeciesInterviewReadContentLevelsIndividualHigherFindSuccessfullyTextHumanResultsTemplate
Variants5
  • Three transcript variants encoding two distinct isoforms have been identified for this gene. (wikipedia.org)
  • Confidently characterise and quantify full-length RNA transcripts, splice variants, and fusions using short to ultra-long fragment nanopore sequencing. (nanoporetech.com)
  • Alternative splicing results in multiple transcript variants. (nih.gov)
  • At least two transcript variants encoding different isoforms have been found for this gene. (nih.gov)
  • Alternatively spliced transcript variants that encode the same protein have been described. (genscript.com)
Isoforms11
  • In order to investigate representative mRNA transcript isoforms to be utilized as transcriptome analysis references, we utilized GTEx data to establish a top-ranked transcript isoform expression data resource for human protein-coding genes. (nature.com)
  • Intriguingly, some of the top-ranked transcripts are noncoding splicing isoforms, which imply diverse gene regulation mechanisms. (nature.com)
  • Comprehensive investigation on the tissue expression patterns of top-ranked transcript isoforms is crucial. (nature.com)
  • Thus, we established a web tool to examine top-ranked transcript isoforms in various human normal tissue types, which provides concise transcript information and easy-to-use graphical user interfaces. (nature.com)
  • Investigation of top-ranked transcript isoforms would contribute understanding on the functional significance of distinctive alternatively spliced transcript isoforms. (nature.com)
  • One major difficulty confronted is complex alternatively spliced transcript isoforms in human protein-coding genes. (nature.com)
  • These studies provided us more information on the spliced transcript isoforms of protein-coding genes as well as more understanding on their expression profiles and translated protein products in human tissues and diseases. (nature.com)
  • We show that an incomplete annotation catalog can have a detrimental effect on the ability to detect differential transcript usage in transcriptomes with few isoforms per gene and that isoform-level prefiltering can considerably improve false discovery rate control. (biomedcentral.com)
  • In particular, DTU considers changes in the proportions of the isoforms of a gene that are expressed as opposed to changes of the individual transcript levels. (biomedcentral.com)
  • Several ER isoforms have been described, but transcript annotation in public databases is incomplete and inconsistent, and functional differences are not well understood. (lu.se)
  • METHODS: We have analysed short- and long-read RNA sequencing data from breast tumours, breast cancer cell lines, and normal tissues to create a comprehensive annotation of ER transcripts and combined it with experimental studies of full-length protein and six alternative isoforms. (lu.se)
Isoform2
  • In particular, the tximport pipeline offers the following benefits: (i) this approach corrects for potential changes in gene length across samples (e.g. from differential isoform usage) (Trapnell et al. (bioconductor.org)
  • differential transcript (or isoform ) usage (DTU). (biomedcentral.com)
MRNA transcript2
  • Genes are typically interspersed with several noncoding regions called introns that interrupt the exons that make up the final mRNA transcript. (scienceboard.net)
  • The initial mRNA transcript, which is called a pre-mRNA molecule, includes both introns and exons, and the process of RNA splicing removes the introns to leave a mature mRNA transcript (composed only of exons) to be translated into protein. (scienceboard.net)
Episode transcript1
  • The full episode transcript for Harlem On My Mind: Abram Hill. (msnbc.com)
Protein12
  • With considerable accumulation of RNA-Seq transcriptome data, we have extended our understanding about protein-coding gene transcript compositions. (nature.com)
  • However, alternatively compounded patterns of human protein-coding gene transcripts would complicate gene expression data processing and interpretation. (nature.com)
  • Distinctive tissue specific expression profiles and modulations could be observed for individual top-ranked transcripts of protein-coding genes. (nature.com)
  • Protein-coding transcripts or genes do occupy much higher expression fraction in transcriptome data. (nature.com)
  • Therefore, information regarding reference annotated mRNA transcripts of respective human protein-coding genes would be beneficial for biomedical researches and pathological sequence variation analyses. (nature.com)
  • Mesoderm-specific transcript homolog protein is a protein that in humans is encoded by the MEST gene. (wikipedia.org)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • Non-protein coding transcript. (h-invitational.jp)
  • Only about 10% to 20% of SMN2 gene transcripts are spliced into mature mRNAs coding for full-length SMN, while the remainder result in the deletion of exon 7 to encode a truncated version of the protein called SMNΔ7. (scienceboard.net)
  • The truncated form is degraded within the cell almost immediately, but the small percentage of SMN2 mRNAs which are successfully translated into full-length SMN protein can lessen the severity of SMA (caused by the absence of a functional SMN1 gene). (scienceboard.net)
  • The orally bioavailable, small-molecule drug modifies the splicing pattern of SMN2 mRNA to include exon 7, thereby increasing the expression of the full-length SMN protein. (scienceboard.net)
  • As a result, mRNAs transcribed from the SMN1 gene are translated into full-length SMN protein, while most of those transcribed from the SMN2 gene are translated into a truncated protein, SMNΔ7. (scienceboard.net)
Genomic1
  • Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. (novusbio.com)
CDNA2
  • First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
  • High-throughput sequencing of cDNA fragment populations, commonly known as RNA-seq, has rapidly become a default standard for profiling the composition of a transcriptome and quantifying the expression of individual transcriptional units (such as genes, transcripts, or exons). (biomedcentral.com)
Genes2
  • Both genes showed an increase in transcript levels until 36 h after explant inoculation, and a subsequent down-regulation, before the initiation of exponential growth. (frontiersin.org)
  • DcAOX genes' were further explored in a plant pot experiment in response to chilling, which confirmed the early AOX transcript increase prior to the induction of a specific anti-freezing gene. (frontiersin.org)
Genome annotation1
  • 2021) Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research. (oeaw.ac.at)
Differential5
  • Accurately analyse differential gene expression and transcript usage. (nanoporetech.com)
  • Charlotte Soneson, Michael I. Love, Mark D. Robinson (2015): Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences. (bioconductor.org)
  • From an analysis perspective, there are several ways to approach RNA-seq data to unravel differential transcript usage, such as annotation-based exon-level counting, differential analysis of the percentage spliced in, or quantitative analysis of assembled transcripts. (biomedcentral.com)
  • Count-based methods generally perform well in the detection of differential transcript usage. (biomedcentral.com)
  • It is important to distinguish DTU from gene-level differential expression and from transcript-level differential expression (DTE). (biomedcentral.com)
Yields2
  • The syntheiss of high yields of full-length reverse transcripts of globin mRNA. (wikidata.org)
  • The HiScribe SP6 RNA Synthesis Kit from NEB is faster and achieves higher yields for RNAs of various lengths. (neb.com)
Abundance3
  • Import and summarize transcript-level abundance estimates for transcript- and gene-level analysis with Bioconductor packages, such as edgeR , DESeq2 , and limma-voom . (bioconductor.org)
  • We begin by locating some prepared files that contain transcript abundance estimates for six samples, from the tximportData package. (bioconductor.org)
  • The transcript ID must be the same one used in the abundance files. (bioconductor.org)
Variant2
  • Homo sapiens glia maturation factor gamma (GMFG), transcript variant 1, mRNA. (genscript.com)
  • Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. (novusbio.com)
Accumulation1
  • Stress-Induced Accumulation of DcAOX1 and DcAOX2a Transcripts Coincides with Critical Time Point for Structural Biomass Prediction in Carrot Primary Cultures (Daucus carota L. (frontiersin.org)
Experimental1
  • A notable application is the detection of changes in transcript usage between experimental conditions. (biomedcentral.com)
Synthesis1
  • This kit is suitable for synthesis of high yield RNA transcripts and for incorporation of cap analogs (not included) or modified nucleotides (not included) to obtain capped, biotin-labeled or dye-labeled RNA. (neb.com)
Gene-level1
  • Transcripts need to be associated with gene IDs for gene-level summarization. (bioconductor.org)
Podcast1
Transcriptome2
  • Even with the increasing sequencing depth of transcriptome studies, elucidating the full extent of transcriptome depth and tissue variation is still a challenging effort ahead. (nature.com)
  • Using deeply sequenced full-length mRNA libraries of oat cultivar Ogle-C, a de novo high-quality and comprehensive oat seed transcriptome was assembled. (usda.gov)
Extent1
  • The extent of this transcript is supported by transcript alignments. (novusbio.com)
Clips1
  • The new special uses clips from a variety of full-length interviews from Eyes on the Prize, which are stored at the Washington University Libraries as part of the Henry Hampton/Blackside Inc. collection . (wustl.edu)
Functional1
  • In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
Research3
  • VectorBuilder offers a full spectrum of gene delivery solutions covering virtually all research and clinical needs from bench to bedside. (bio-itworld.com)
  • Since journals typically insist on reducing the length of papers, published articles are often highly condensed versions of the full results of a research effort. (lu.se)
  • A collection of policy documents, parliamentary transcripts, government guidance and think tank research. (lu.se)
Expression of full-length2
  • BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)
  • In contrast, the ubiquitous expression of full-length mHTT in the BACHD hypothalamus moderately affects the transcriptomic profile. (lu.se)
Species1
  • Both common and uniquely modulated transcripts and perturbed pathways were identified for the metal species. (cdc.gov)
Interview2
  • Learn more about this health topic in the full-length interview. (utah.edu)
  • Full-length interview with Bobby Seale produced for "Eyes on the Prize: America at the Racial Crossroads 1965-1985. (wustl.edu)
Read1
  • Click on the transcript button to read the description. (ackland.org)
Content1
  • Cancer.Net podcasts are edited for content and length. (cancer.net)
Levels1
  • Cd levels remained elevated over the length of the experiment, while Cr levels declined. (cdc.gov)
Individual1
  • Each unaffected full sibling of an affected individual has a 67% likelihood of being a carrier. (medscape.com)
Higher1
  • Transcript expression showed higher correlations between adjacent time points. (usda.gov)
Find1
Successfully1
  • Pro-French Secretary of State Thomas Jefferson , already at odds with the neutral Federalist administration, successfully insisted that Genet be accorded full diplomatic recognition. (encyclopedia.com)
Text2
  • Full-text journals, reference books and conference papers. (lu.se)
  • Please note that the text below is not a full transcript and has not been copyedited. (medscape.com)
Human1
  • No unspliced RHO transcripts could be detected in RNA from human retina. (molvis.org)
Results1
  • While the SMN1 gene's pre-RNA is always spliced to include exon 7, a point mutation in the SMN2 gene results in the deletion of exon 7 from the majority of mature mRNA transcripts. (scienceboard.net)
Template1
  • Splicing of full-length RHO is more efficient than splicing of the minigene, and assays using a full-length template more accurately mimic splicing in photoreceptors. (molvis.org)