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  • Fourier Transfor
  • Herein, we show that Fourier transform infrared (FTIR) linewidth analysis of noninvasive 1-13C═18O labels can be used to obtain a reliable measure of the local polarity, even in a highly multiphasic system, such as a membrane protein. (wisc.edu)
  • The book describes basic techniques of Fourier-Transform Infrared (FTIR) and Raman scattering spectroscopies. (helion.pl)
  • amino
  • first, a local scale, in which a specific amino aci9-s residue is affected, altering the binding of CO to Mb through a steric interaction, and second, a global scale, in which hydrogen bonds are disrupted in the bulk of the protein structure, causing a slight change in the conformation of the protein as a whole. (illinois.edu)
  • The potentiality of FTIR-DS is fully exploited when the structure of the protein is known and at least some of the bands in the difference spectrum have been assigned to a vibration of a given chemical moiety of a specific amino acid or cofactor inside the protein. (hindawi.com)
  • We have developed a method to incorporate unnatural amino acid residues into the oxygen-evolving center. (gatech.edu)
  • This approach will provide a new dimension in protein engineering, enabling the replacement of native amino acid residues with custom designed residues. (bu.edu)
  • The sequences of IDPs have low hydrophobicity, and they are substantially enriched in polar (such as R, G, Q, S, P, E, and K) and structure-breaking (such as G and P), disorder-promoting amino acid residues [ 3 ]. (mdpi.com)
  • lipid
  • The film was scanned by FTIR, and lipid (1725 cm -1 ) and protein (1545 cm -1 ) peaks were used for the respective quantifications. (arvojournals.org)
  • Less than half the levels of both lipid and protein were recovered from lenses used with the rub regimen (CER) than with the no-rub regimen (OFX). (arvojournals.org)
  • Average lipid levels were 3.4µg/lens with rub (CER) and 7.2µg/lens with the no-rub (OFX), while protein levels were 0.7µg/lens with rub (CER) and 2.0µg/lens with no-rub (OFX). (arvojournals.org)
  • FTIR analysis of clinically worn Focus Night & Day silicone hydrogel lenses showed significant improvement of lens cleanliness with regards to both lipid and protein by use of a rub-regimen product (CER) versus a no-rub regimen product (OFX). (arvojournals.org)
  • FTIR was assessed to be a viable means of quantifying lipid and protein as general categories of lens deposits. (arvojournals.org)
  • We show that in the Influenza M2 H+ channel, residues that line the pore are located in an environment that is as polar as fully solvated residues, while residues that face the lipid acyl chains are located in an apolar environment. (wisc.edu)
  • Protein, chitin, and lipid characteristic spectral features were detected using both methods. (degruyter.com)
  • membrane proteins
  • It is also an excellent system to study matrix effects on the dynamics of membrane proteins and ET processes [ 3 ]. (hindawi.com)
  • The group focuses primarily on rhodopsins, a large family of integral membrane proteins involved in animal vision and photo-sensing and ion transport in bacteria and algae. (bu.edu)
  • In this project, the biophysics group is developing new methods based on molecular genetics and advanced biophysical techniques that will provide the capability to modify membrane proteins at the molecular level. (bu.edu)
  • Membrane proteins facilitate many key cellular processes, including energy transduction, ion transport, and signal recognition. (bu.edu)
  • time-resolved
  • The RC has also been studied by time-resolved FTIR-DS ([ 8 - 12 ] and references therein) and used as a "case study" to develop and test new data analysis techniques ([ 12 , 13 ] and references therein). (hindawi.com)
  • aromatic
  • The first phase, from 270 up to 310 °C, shows monosaccharides, protein fragments and aromatic compounds. (uu.nl)
  • The EI and CI experiments fail to discriminate between the pyrolysis products of polysaccharides and proteins between 250 and 400 °C. FTIR shows in this temperature range the development of an aromatic network. (uu.nl)
  • organic
  • For example, we recently reported the discovery of organic protein remains in fossils. (icr.org)
  • A completely different class of fossils holds remnants of animal biochemicals like proteins, pigments, and DNA that minerals never replaced, and lab tests indicate that these organic tissue components could not last a million years-that's what we're talking about. (icr.org)
  • These conditions include amorphous, dehydrated protein powders, suspensions of such in organic solvents, and encapsulated in polymer matrices. (irdg.org)
  • Such encapsulation involves the exposure of proteins to very denaturing conditions, such as dehydration and organic solvents. (irdg.org)
  • Previous to this study, burned limestone rocks were submitted to PaleoResearch for organic (FTIR) analysis from these same sites, as well as from a few other sites in the general area (Logan 2010). (tdar.org)
  • mixtures
  • Functional compounds between soya, wheat, corn protein isolates, and konjac glucomannan (KGM) and these dough mixtures were investigated by FTIR studies. (academicjournals.org)
  • The presence of overlapping absorption bands at 3435.95 and3488.99 cm -1 which were assigned to -OH and NH 2 stretch vibration, from protein matrix and -OH vibration from KGM intermingled to form a smooth surface of SEM image of the dough mixtures. (academicjournals.org)
  • proton
  • represents a model system in bioenergetics, especially suited to investigate electron transfer (ET), proton transfer, and quinone redox chemistry in proteins [ 2 ]. (hindawi.com)
  • biological
  • Research in my group is focused on how the dynamic and responsive protein matrix facilitates biological catalysis. (gatech.edu)
  • Lipases synthesized by microbes play important roles as natural functional proteins in which their type and function in bacteria, actinomycetes, and fungi may differ mainly depending on its constitutive genetic makeup and biological evolution in order to optimize their behavioural condition in complex environment under normal-stress condition as well as for their living and survival [ 8 - 12 ]. (hindawi.com)
  • However, as early as in the 1990s, it was reported that there is another class of proteins, which have no well-defined structures under physiological conditions, but still have biological functions [ 1 , 2 ]. (mdpi.com)
  • processes
  • Our study suggests that peas should have been heated up to least 310 °C before their residues survive natural degradation processes. (uu.nl)
  • changes
  • Millisecond changes to protein structure can be studied by observing the CD signal at an informative wavelength during the course of a stopped flow reaction. (olisweb.com)
  • vibrational
  • After discussing the vibrational modes of the peptide bond, the main analytical procedures employed are introduced, and limitations of the FTIR methodology are discussed. (irdg.org)
  • study
  • Both these parameters provide important information about the stability of the protein under study. (olisweb.com)
  • Structures
  • Intrinsically disordered proteins (IDPs) are proteins that usually do not adopt well-defined native structures when isolated in solution under physiological conditions. (mdpi.com)
  • bulk
  • Peas (Pisum sativum L.) consist mainly of cotyledons and their bulk material is starch and proteins. (uu.nl)
  • provide
  • Therefore, why would anyone use a methodology that typically does not provide detail information but rather an "overview" picture of global structural arrangements in proteins? (irdg.org)
  • results
  • The FTIR results show significant (p C-H ), alkanes ( C-H ), alcohol ( O-H ) and unsaturatedesters ( C-O ). (scirp.org)
  • Nair and Pradeep used Lactobacillusstrains with silver ions in the biosynthesis of nanoparticles and they alluded to the fact that if the lactic acid bacteria metabolites in whey protein are mixed with silver ion with results to the nucleation of the silver ions hence formation of AgNP. (scirp.org)
  • The results showed that fragments-CONH 2 , were assigned to absorption bands in the region of 1634 - 1650 cm -1 , and fragments-carboxyl groups of protein matrix were exhibited at the region of 3235 - 3460 cm -1 . (academicjournals.org)
  • Finally, some recent results on lyophilized proteins are used to exemplify how the developed tools were employed to address important scientific issues. (irdg.org)
  • show
  • In contrast to QUA1, another Golgi-localized protein required for HG-synthesis, QUA2 does not show sequence similarity to glycosyltransferases, but instead contains a putative methyltransferase (MT) domain. (wiley.com)
  • The resulting solid products of the heating process still show the original markers for polysaccharides and proteins up to 270 °C. Concurrently three phases can be considered, each characterized by its own products. (uu.nl)
  • decade
  • Over the last decade, nanoparticles used as protein carriers have opened new avenues for a variety of biomedical applications. (hindawi.com)
  • Over the past decade, many useful protein ligands have been identified through screening of peptoid libraries. (jove.com)
  • important
  • One important application of this work is the development of optogenetic proteins for use in neuroscience research, such as elucidating the functioning of complex neural circuits and understanding the basis for neurodegenerative diseases. (bu.edu)
  • state
  • In a recent work [ 15 ] it has been shown that the hydration state of the RC controls the protein dynamics associated to ET reactions. (hindawi.com)
  • However, proteins are susceptible to solid-state aggregation under such conditions . (irdg.org)
  • class
  • The disulfide connectivity pattern, together with data on the CD81 binding site and reported E2 deletion mutants, enabled the threading of the E2e polypeptide chain onto the structural template of class II fusion proteins of related flavi- and alphaviruses. (prolekare.cz)
  • found
  • Residues of heated peas, usually called carbonized peas, are found in the archaeological record. (uu.nl)
  • shown
  • The water oxygens are shown in purple, the Gly34 residues in green, and the amide oxygen in red. (nih.gov)
  • The His37 residues are shown in light blue, and the ring nitrogens ( N δ and N ε ) are shown in dark blue. (nih.gov)