• Ovarian tissue cryopreservation is a method of maintaining fertility in which the outer layer of an ovary, which contains a large number of young eggs, is removed and frozen for future use. (fertilitycrete.gr)
  • Directional solidification demonstrates benefits in adhered cells and native tissues cryopreservation through controlled heat transfer. (huji.ac.il)
  • The results suggest that directional freezing becomes a viable option for cryopreservation in tissue engineering applications. (huji.ac.il)
  • When you're talking about temperatures that low, you can freeze off freaking anything. (vice.com)
  • Frostbite, the most common type of freezing injury, is defined as the freezing and crystalizing of fluids in the interstitial and cellular spaces as a consequence of prolonged exposure to freezing temperatures. (medscape.com)
  • Chilblains are caused by the repeated exposure of skin to temperatures just above freezing to as high as 60°F. The cold exposure causes damage to the groups of small blood vessels in the skin. (cdc.gov)
  • Exposure to below-freezing temperatures puts any part of the body at risk of frostbite. (msdmanuals.com)
  • Initially, Gomez and his colleagues simply wanted to understand how some plants, for example carrots, survive the freezing temperatures of winter. (lu.se)
  • Schoenberg Fejzo M, Slamon DJ: Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. (wikipedia.org)
  • In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
  • This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites. (unibo.it)
  • The stored ovarian tissue can be transplanted orthotopically (in the rest of the ovary, ovarian cortex or in the wider joint), heterotopically (subcutaneous abdominal tissue, anterior abdominal wall, just below the peritoneum or rectum), recent studies have been reported to fully mature in vitro. (fertilitycrete.gr)
  • A complete in vitro spermatogenesis has been obtained from mouse prepubertal testicular tissue, although with low efficiency. (elifesciences.org)
  • Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
  • Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
  • It has been previously shown that supplementing organotypic culture media with retinol improves the in vitro production of spermatids and spermatozoa in prepubertal mouse testicular tissues cultured at a gas-liquid interphase ( 5 , 7 ). (elifesciences.org)
  • Cytomics Frozen section procedure MicroArray and Gene Expression (MAGE) Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. (wikipedia.org)
  • As expected, the colder the freezing procedure, the less damage the process generally produced. (magritek.com)
  • The organotypic culture procedure, which preserves testicular tissue architecture, microenvironment and cell interactions, has been used successfully to obtain spermatozoa from fresh or frozen/thawed mouse prepubertal testicular tissues ( 3 - 6 ). (elifesciences.org)
  • Pioneering doctors had implanted previously frozen ovarian tissue into an American woman, Margaret Lloyd-Hart, but the procedure was not a success. (bbc.co.uk)
  • In the process of freezing the ovarian tissue, a portion of an ovary is usually removed by laparoscopy. (fertilitycrete.gr)
  • In the laboratory, the outer layer of the ovary (called the ovarian cortex) is cut into small strips and is frozen. (fertilitycrete.gr)
  • While men whose fertility is threatened can elect to have semen frozen for later use, at the moment, the preservation - and reactivation - of eggs, and tissue from the ovary, is far more problematic. (bbc.co.uk)
  • Frozen tissue cores with 2 mm diameter from the regions of interest are removed from frozen tissue OCT blocks at different freezing temperature since each tissue type has their own temperature preference at frozen stage. (wikipedia.org)
  • Samples were directionally frozen in a sandwich format either in 10% DMSO or culture medium with the sample movement at 30 μm/s through the predetermined temperature gradients along a 2.6 mm slit. (huji.ac.il)
  • The fingers, toes, ears, and nose-the skin structures most at risk for frostbite-contain multiple arteriovenous anastomoses that allow shunting of blood in order to preserve core temperature at the expense of peripheral tissue circulation. (medscape.com)
  • Overview of Cold Injuries The skin and the tissues under it are kept at a constant temperature (about 98.6° F, or 37° C) by the circulating blood and other mechanisms. (msdmanuals.com)
  • Some of these studies have used fresh spinal meninges obtained from living animals, [1-4] whereas others have relied on cadaveric human tissue previously frozen for variable periods of time before study. (asahq.org)
  • Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to allow simultaneous histological analysis. (wikipedia.org)
  • Ovarian tissue freezing is also an important method of maintaining fertility for young girls. (fertilitycrete.gr)
  • Freezing of prepubertal testicular tissue containing spermatogonia is a fertility preservation option proposed to prepubertal boys with cancer prior to highly gonadotoxic treatments. (elifesciences.org)
  • Frozen human ovarian tissue - a potential fertility lifeline for hundreds of UK women - has been successfully thawed and revived in mice. (bbc.co.uk)
  • Dr Fishel commented that while frozen egg fertility treatments seemed to have the greatest current potential in the short term, there were still safety concerns about possible genetic damage within the egg during the dehydrating and freezing process. (bbc.co.uk)
  • However, as for the effect of the pretreatment method prior to freezing for strawberry tissues, most researchers have focused only on one of the textural parameters, i.e. fracture stress that is considered as a textural index reflecting the cell wall hardness. (ishs.org)
  • For this reason, measurement of tissues that underwent freezing and defrosting may provide researchers with more flexibility in setting measurement campaigns, thus speeding up the development of microwave-based biomedical devices. (iscte-iul.pt)
  • Further, we investigated the effect of the pretreatment with the CaCl 2 solution, which is well known as a texture preserving technique for freeze-thawing fresh strawberry. (ishs.org)
  • At Imperial College London , a method combining 3D printing and cryogenics may have the answer to replicating the texture of soft tissues in the body, and potentially fooling parts of our lungs and brain. (3dprintingindustry.com)
  • We observe a slight increase-less than 10%-in complex permittivity of high-water-content tissues (muscle and liver) after defrosting, and negligible effect on fat tissues. (iscte-iul.pt)
  • When fresh strawberry tissue is frozen and thawed, the tissue suffers from remarkable softening like other vegetables. (ishs.org)
  • still, diffusion cell studies using fresh and frozen meningeal tissues continue to be submitted to anesthesia journals for publication (personal observation, C.M.B.). To address this concern, we directly compared the permeability of morphine through fresh and dry-frozen spinal meninges. (asahq.org)
  • We measured the permeability of morphine through fresh spinal meninges obtained from anesthetized monkeys and then repeated this measurement through the same tissues after they were dry-frozen at -20 [degree sign]C. (asahq.org)
  • The methods for obtaining fresh meningeal tissue and for determining permeability coefficients have been described in detail previously. (asahq.org)
  • As a result, cross-contamination of cytosolic and nuclear fractions from fresh/frozen tissues remains a major problem (see references 1 and 2 below). (inventbiotech.com)
  • This novel Cytosolic and Nuclear Extraction Kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contamination for fresh and frozen tissue samples. (inventbiotech.com)
  • second, determine if defrosted tissue dielectric properties differ from those of fresh tissues. (iscte-iul.pt)
  • Imagine a fresh, crunchy salad that has been… frozen and defrosted? (lu.se)
  • Additionally, the diffusion of water molecules in tissue was measured by using NMR to evaluate the degradation of cell membrane. (ishs.org)
  • When the frozen ovarian tissue is re-implanted in the body and regains its function, the immature eggs that are frozen inside the tissue begin to develop normally and become recoverable in an IVF process. (fertilitycrete.gr)
  • The tissue was implanted into mice, and immature follicles within the tissue, which are supposed eventually to release the eggs for fertilisation, began to develop in a similar way to conventional ovarian tissue. (bbc.co.uk)
  • In this study, we developed a method to analyze damages on strawberry tissue taking the membrane degradation into account. (ishs.org)
  • These measurements are often not practical, as tissue degradation restricts the time available between tissue excision and dielectric measurements. (iscte-iul.pt)
  • The majority of current commercial kits are designed to treat cultured cells and tissues similarly, not paying sufficient attention to the structural difference between the two. (inventbiotech.com)
  • Another piece of Australian research also found that mouse ovarian follicles could be frozen and thawed, then similarly developed under laboratory conditions. (bbc.co.uk)
  • this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. (wikipedia.org)
  • Scientists who have been developing antibodies for therapeutic purpose all need their preliminary results from frozen tissues to get approval from FDA. (wikipedia.org)
  • A team of scientists from Melbourne, Australia, has now demonstrated that frozen human ovarian tissue, once thawed, can grow in a "normal" fashion. (bbc.co.uk)
  • Nonfreezing tissue injuries include frostnip, immersion foot, and chilblains. (msdmanuals.com)
  • In order to thoroughly evaluate different freezing protocols, a method to characterize the extent and distribution of damage from freezing is needed. (magritek.com)
  • Since frozen tissue sections don't go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen tissues retain their native characteristics much more than in paraffin embedded tissues. (wikipedia.org)
  • The main challenge of synthetic tissue is fabricating it in a way that tricks the body into believing that it has been there all along. (3dprintingindustry.com)
  • The setup was able to successfully create 3D complex geometrical structures, with an average compressive stiffness of O(1) kPa (0.49 ± 0.04 kPa stress at 30% compressive strain) and therefore mimics the mechanical properties of the softest tissues found in the human body (e.g. brain and lung). (3dprintingindustry.com)
  • The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. (inventbiotech.com)
  • This study reports useful information on the limits of the organotypic culture of neonatal mouse testes, which has been regarded as an experimental strategy that can be extended to humans in the clinical setting for the conservation and subsequent re-use of testicular tissue. (elifesciences.org)
  • Shoulder pain may be severe and sudden for injuries such as a fracture or sprain or conditions such as a frozen shoulder. (medicalnewstoday.com)
  • Nonfreezing Tissue Injuries In nonfreezing tissue injuries, parts of the skin are chilled but not frozen. (msdmanuals.com)
  • The associated decrease in blood flow does not deliver sufficient heat to the tissue to prevent the formation of ice crystals. (medscape.com)
  • As a result, it was revealed that both cell wall and cell membrane got damaged, so that the remarkable tissue softening was induced. (ishs.org)
  • However, for strawberry tissue, damage on cell membrane was found to be dominant in textural softening. (ishs.org)
  • It was also clarified that the pretreatment with the CaCl 2 solution would suppress the decrease of fracture stress reflecting cell wall hardness but could not improve initial modulus reflecting cell membrane tension after freeze-thawing. (ishs.org)
  • As a conclusion, it was found to be necessary to control the damage on cell membrane to prevent the softening of strawberry tissue after freeze-thawing. (ishs.org)
  • In conclusion, this study reports the failure of adult Leydig cell development and altered steroid production and signaling in tissue cultures. (elifesciences.org)
  • Before freezing and 24 h after thawing, cell viability was assessed using live-dead assay. (huji.ac.il)
  • Sucrose in the freezing solution is used to draw water out through the cell membrane. (bbc.co.uk)
  • Other vegetables, such as peas, have a different cell structure and are less affected by being frozen altogether. (lu.se)
  • Therefore, many scientific experimental results from formalin fixed tissues are not reliable. (wikipedia.org)
  • Results show non-uniform damage throughout the fillet, with tissue damage due to freezing concentrated in the center of the fillet. (magritek.com)
  • Freezing of fish is an important processing method that can extend the shelf life of the product but can also lead to significant damage to the tissue if performed incorrectly. (magritek.com)
  • A unique method of freezing vegetables and fruit that keeps cells alive has been developed, and patented, at Lund University in Sweden. (lu.se)
  • Using input from two other research areas - how cells can be treated with electrical pulses, and how tissue behaves in a vacuum - the method was developed. (lu.se)
  • Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. (inventbiotech.com)
  • Within randomly orientated PCL-PLA fibers, HeLa cells exhibited typical shape and attachment with higher than 90% viability prior to freezing. (huji.ac.il)
  • While up to 80% of HeLa cells were alive on fiber mats after freezing using DMSO with or without directional solidification step. (huji.ac.il)
  • The demonstrated controlled freezing may assist optimizing the freezing of more sensitive cells. (huji.ac.il)
  • The vegetables are frozen after a few hours, since the cells have been put under stress. (lu.se)
  • Magnetic resonance imaging (MRI) was tested as a technique to map and quantify tissue damage from freezing in fish. (magritek.com)
  • Areas of damage appear as bright clusters in the muscle tissue. (magritek.com)
  • This suggests that surface limited methods, such as hyperspectral imaging, may not fully capture the extent of damage due to freezing and thawing. (magritek.com)
  • Worst-case scenario, he has permanent nerve damage, he has skin tissue that can fall off and slough off. (vice.com)
  • Water left in the egg causes damage to its fragile contents during freezing. (bbc.co.uk)
  • Unlike the freezing of eggs and embryos, where only one cycle can take several weeks to complete, the ovarian tissue can be frozen in a very short time. (fertilitycrete.gr)
  • An important advantage of freezing ovarian tissue is the number of eggs that can be frozen. (fertilitycrete.gr)
  • And in what could be more good news for women threatened with infertility, a new technique appears to have greatly increased the number of frozen human eggs which can be subsequently fertilised. (bbc.co.uk)
  • Only 30 babies have been born worldwide using frozen eggs - the success rate is extremely low. (bbc.co.uk)
  • The Bologna team doubled or even tripled the concentration of sucrose, and found great improvements in the proportion of eggs which survived the freezing process. (bbc.co.uk)
  • Dr Rafaella Fabbri, who led the research, said: "It is very important that we improve the possibilities for women to become pregnant after having their eggs frozen. (bbc.co.uk)
  • Then all the frozen tissue cores are inserted in a recipient OCT frozen block in a precisely spaced, array pattern. (wikipedia.org)
  • We are reaching out to you today to reassure you that during these difficult times that all of your cryopreserved tissues in storage at Hanabusa IVF are being very well taken care of. (hanabusaivf.com)
  • People aged 40-60 are most likely to develop frozen shoulder, and it is four times more common in females than males. (medicalnewstoday.com)
  • Tissue microarrays from frozen tissues-OCT technique. (wikipedia.org)
  • However, it is the restoration of working ovarian tissue, even allowing natural conception to take place, that is described as the "holy grail" in this field. (bbc.co.uk)
  • However, a team from the University of Bologna, Italy, say they have found a way of improving the egg's chances of surviving the freezing process. (bbc.co.uk)
  • The wet feet lose heat faster and the circulation begins to shutdown, resulting in the skin tissue beginning to die. (cdc.gov)
  • As a result, we measure the dielectric properties of ten samples of chicken muscle, bovine liver, and bovine fat, each before and after freezing (up to 14 days) and defrosting. (iscte-iul.pt)
  • HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over 1 year of freezing at -80 A degrees C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011-2012. (unibo.it)
  • Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. (unibo.it)
  • To provide repeatable, objective classification, image analysis using a convolutional neural network was then performed on the MRI data to identify regions of damaged tissue. (magritek.com)
  • You need blood flow to maintain the health of the tissue, the nerves, and any of the surrounding structures. (vice.com)
  • Because cold causes blood vessels to narrow, tissue that is near the frozen area but not itself frozen may be damaged as a result of the decreased blood flow. (msdmanuals.com)
  • These clots may limit blood flow so much that the tissue dies. (msdmanuals.com)
  • When blood flow returns to the affected area, the damaged tissues release a number of chemical substances that promote inflammation. (msdmanuals.com)
  • In this letter we study the effect of freezing and defrosting on the dielectric properties of biological tissues. (iscte-iul.pt)
  • Most areas heal over time, but sometimes surgery is required to remove dead tissue. (msdmanuals.com)
  • The follicles within the ovarian tissue did not develop fully. (bbc.co.uk)