AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Ribonuclease T1RibonucleasesRibonuclease, PancreaticGuanosine MonophosphateEndoribonucleasesAspergillus oryzaeRibonuclease HRibonuclease PIodoacetatesOligoribonucleotidesCytidine MonophosphateAconitic AcidGuanine NucleotidesGuanosineBase SequenceKineticsProtein ConformationEscherichia coliProtein DenaturationPlacental HormonesHydrogen BondingTryptophanEnzyme StabilityDinucleoside PhosphatesHydrogen-Ion ConcentrationRibonuclease IIISpectrophotometry, UltravioletBinding SitesMolecular Sequence DataThermodynamicsAspergillusModels, MolecularNucleic Acid ConformationGlycolatesAmino Acid SequenceRNA, TransferRNA, RibosomalMagnetic Resonance SpectroscopySpectrometry, FluorescenceUreaSubstrate SpecificityAmino AcidsEosinophil-Derived NeurotoxinOligonucleotidesGuanineX-Ray DiffractionCrystallizationCircular DichroismElectrophoresis, DiscProtein FoldingProtein BindingHydrogenRNA, ViralPancreasCattleMolecular WeightFree RadicalsStructure-Activity RelationshipExoribonucleasesCrystallography, X-RayGlutamatesRecombinant ProteinsRibonuclease H, Human Immunodeficiency VirusRNARNA, CatalyticStereoisomerism
GrownMediumMedia
Grown2
  • pLB (5 × 10 4 ) or pLUM alone, or as 5:1, 3:1, 1:1, 1:3 or 1:5 pLUM:pLB ratios, were plated in E-free, growth factor (GF)-reduced Matrigel and grown into three-dimensional colonies for 7 days. (medscape.com)
  • Cells were grown in T0 media, consisting of RPMI-based serum-free medium with Chir 99021 (2ug/ml) and Activin A (100 ng/ml), for one day. (nih.gov)
Medium2
  • They were maintained in E-containing medium and remained luminobasal-free. (medscape.com)
  • On days "T1-2", medium was changed to RPMI with BMP4 (0.5 ng/ml), FGF2 (10ng/ml), Activin A (100 ng/ml), and VEGF (10 ng/ml). (nih.gov)
Media1
  • They were maintained in EWD media and remained luminal-free. (medscape.com)