AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth CareGeographicals
Cell FractionationChemical FractionationSubcellular FractionsFractionation, Field FlowCytoplasmCell NucleusMolecular Sequence DataCell CompartmentationCell MembraneAmino Acid SequenceMolecular WeightMicroscopy, ImmunoelectronEndoplasmic ReticulumCytosolCell LineElectrophoresis, Polyacrylamide GelMembrane ProteinsGolgi ApparatusBiological TransportFluorescent Antibody TechniqueProtein TransportTrichomonasKineticsMicroscopy, ElectronIntracellular MembranesBase SequenceCells, CulturedLysosomesRecombinant Fusion ProteinsCloning, MolecularBlotting, WesternCaveolinsMitochondriaMicroscopy, FluorescenceProtein Processing, Post-TranslationalHeLa CellsMicrosomesDigitoninImmunoblottingSaccharomyces cerevisiaeLiverMicrobodiesCarrier ProteinsProteinsChromatography, GelCentrifugation, Density GradientSequence Homology, Amino AcidProtein BindingChromatographyCaveolin 1Cytoplasmic GranulesTransfectionVacuolesChromatography, Ion ExchangeCOS CellsMicroscopy, ConfocalPrecipitin TestsRecombinant ProteinsBacterial ProteinsPolyribosomesMutationChemistry Techniques, AnalyticalDetergentsFluorescent Antibody Technique, IndirectCell SeparationDNA, ComplementaryBrefeldin ARNA, MessengerFungal ProteinsEscherichia coli3T3 CellsProtein Sorting SignalsSulfur IsotopesCricetinaeSaccharomyces cerevisiae ProteinsProtein BiosynthesisPeroxisomesCentrifugation, IsopycnicPhosphorylationSolubilityNuclear ProteinsFibroblastsViral ProteinsEndocytosisTritiumTissue DistributionNuclear EnvelopePlant ExtractsPhosphoproteinsCattlePeptidesRabbitsPlasmidsHydrogen-Ion ConcentrationChromatography, DEAE-CelluloseProtein PrecursorsGreen Fluorescent ProteinsCercopithecus aethiopsTime FactorsProtein Structure, Tertiary
Eukaryotic cellsSubcellularDescriptorProteinsHeLaNucleusCompartmentsPeopleSpecificCancer cellNormalSystems
Eukaryotic cells3
  • Eukaryotic cells are complex, well-ordered, and highly structured systems. (qiagen.com)
  • The Cell Compartment Kit is designed for fast and easy subcellular fractionation of intact eukaryotic cells and tissue. (qiagen.com)
  • The hallmark of eukaryotic cells is their compartmentalization into distinct membrane-bound organelles. (elifesciences.org)
Subcellular2
Descriptor1
  • Cell Fractionation" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
Proteins9
  • By sequential addition of different extraction buffers to a cell pellet, proteins in the different cellular compartments can be selectively isolated. (qiagen.com)
  • The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues. (qiagen.com)
  • Localization of proteins in cells under different growth conditions. (qiagen.com)
  • We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
  • Proteins are the tools and machines of the cell, and each organelle has its own set of proteins that it requires to work correctly. (elifesciences.org)
  • Each cell contains ten or more organelles, and several thousand different types of proteins. (elifesciences.org)
  • The location of many proteins in a cell is unclear or simply not known. (elifesciences.org)
  • set out to develop a method that reveals the locations of all the proteins in a cell at any given time. (elifesciences.org)
  • The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
HeLa2
  • Micrographs (upper panels) of HeLa cells [A] under normal growth conditions and [B] after 4 hour incubation with staurosporine, an inducer of apoptosis. (qiagen.com)
  • Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)
Nucleus1
  • Results showed that TIGAR was increased and relocated to the nucleus after epirubicin or hypoxia treatment in cancer cells. (nature.com)
Compartments1
  • The interior of every cell is highly organised, and contains many compartments, called organelles, that are dedicated to specific roles. (elifesciences.org)
People3
  • This graph shows the total number of publications written about "Cell Fractionation" by people in this website by year, and whether "Cell Fractionation" was a major or minor topic of these publications. (wakehealth.edu)
  • Below are the most recent publications written about "Cell Fractionation" by people in Profiles. (wakehealth.edu)
  • also want to investigate the differences between healthy cells and cells from people with neurological disorders to understand how such diseases arise. (elifesciences.org)
Specific1
  • Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
Cancer cell1
  • The Cdk5-AMT signal pathway involved in regulation of DDR by TIGAR defines a new role of TIGAR in cancer cell survival and it suggests that TIGAR may be a therapeutic target for cancers. (nature.com)
Normal1
  • were able to compare such a model of the cell under normal circumstances to a model made after the cell had been stimulated with a growth factor. (elifesciences.org)
Systems1
  • The ability to monitor changes in organellar composition would provide a powerful tool to investigate cell biological processes at the systems level. (elifesciences.org)