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Chromatography, GelChromatography, Ion ExchangeMolecular WeightChromatography, AffinityChromatography, High Pressure LiquidGelsElectrophoresis, Polyacrylamide GelChromatographyMolecular Sequence DataKineticsAmino Acid SequenceAmino AcidsSubstrate SpecificityHydrogen-Ion ConcentrationChromatography, LiquidChromatography, DEAE-CelluloseChromatography, GasChemical FractionationIsoelectric FocusingIsoelectric PointEscherichia coliChromatography, Thin LayerChromatography, AgaroseMacromolecular SubstancesCattleCell FractionationFiltrationSolubilityProtein BindingCarbohydratesUltracentrifugationRecombinant ProteinsTemperatureRabbitsMass SpectrometryHot TemperatureEnzyme StabilityBase SequenceCloning, MolecularBacterial ProteinsDimerizationImmunodiffusionOligosaccharidesProtein ConformationGlomerular Filtration RateCarbohydrate SequenceChemistryHydroxyapatitesSequence Homology, Amino AcidCentrifugation, Density GradientHydrolysisChemical PhenomenaTrypsinPeptide FragmentsGlycoside HydrolasesGlycoproteinsLiverCations, DivalentUltrafiltrationDetergentsCircular DichroismEdetic AcidBinding SitesPeptide HydrolasesRadioimmunoassayPeptidesCarrier ProteinsCytosolAgaricalesMicroscopy, ElectronSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCell LineCell MembraneProteinsDurapatiteBiological AssaySubcellular FractionsEndopeptidasesMagnetic Resonance SpectroscopyElectrophoresis, Gel, Two-DimensionalProtease InhibitorsSwineCross-Linking ReagentsIsoenzymesBlotting, WesternRats, Inbred StrainsPolysaccharidesImmune Serabeta-GlucosidaseCarbohydrate ConformationPhospholipidsProtein DenaturationHyperprolactinemiaSpectrophotometrySpectrometry, FluorescenceStreptomycesLectinsSequence AlignmentKidneyBlood Proteins
AffinitySepharoseAnion exchange chromatographySeparationProteinsField-flow fractionPermeationEthanolAmmonium SulphateSilica gelCohnElectrophoresisChromatographic methodsIsolationPurificationProteinCelluloseAnalyticalColumnsMolecular weightsLaboratoriesEnzymeMethodsPolymersBufferHomogeneitySizeSampleAnalysisDevelopmentMethodExchangeMedicineProductsFindProcess
Affinity6
Sepharose2
  • There are three main steps that combine Cohn fractionation with chromatography: 1) factors I, II, and III are removed via cold ethanol fractionation, 2) Sepharose fast flow ion exchange and sepharose fast flow chromatography procedures are run, and 3) gel filtration is run. (wikipedia.org)
  • Their modernized albumin purification process consists of five steps: Starting material is plasma that has been pretreated by centrifugation, A round of gel filtration is run, ion exchange on DEAE Sepharose is run to bind the albumin to the column, Albumin is eluted with a sodium acetate buffer, and Final polishing with gel filtration. (wikipedia.org)
Anion exchange chromatography1
Separation12
  • Chromatography is a physical method of separation that distributes the components you want to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. (wikipedia.org)
  • Gel permeation chromatography gpc is a chromatographic technique in which the separation obtained is a function of molecular size. (web.app)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • Gel permeation chromatography gpc is a column separation technique based on a nonionic molecularsieve effect and, in contrast to other chromatographic. (web.app)
  • Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. (gbiosciences.com)
  • Use for biomaterial separation, fractionation and so on. (nagasaki-u.ac.jp)
  • Due to the AF4 feature to discretionarily alter the resolution power within one separation run, the fractionation of higherorder aggregates and insoluble, precipitated protein was successfully performed. (uni-muenchen.de)
  • Summary, conclusions and prospective 177 Given the background that effective AF4 fractionations are due to differences in analyte size - i.e., in diffusion coefficients -, the separation of equal-sized proteins is prima facie considered to be impractical. (uni-muenchen.de)
  • In this chapter, we first define and classify the membrane separation technology and then comprehensively describe its applications, for instance, component separation, filtration, removal of bacteria, and wastewater treatment in dairy industry. (intechopen.com)
  • Standardized silica gels are widely used in separation processes to purify high value compounds in ton quantities. (emdmillipore.com)
  • Column chromatography is a technique used for the separation of mixtures. (rsc.org)
  • The adoption of on-line fractionation and optimised acquisition protocols utilising ion mobility separation (IMS) technology has significantly increased the scope for protein identifications ten-fold. (biomedcentral.com)
Proteins6
  • Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectively-from the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. (vwr.com)
  • As expected, polyacrylamide gel electrophoresis (PAGE) for native basic proteins confirmed the homogeneity and PAGE in presence of dodecyl sodium sulphate revealed a single 14-kDa polypeptide band. (bvsalud.org)
  • These proteins were discovered and characterized through activity purification, SDS-PAGE analysis, and gel filtration chromatography . (ichacha.net)
  • Yet, the retaining impact of sample-membrane interaction was evidenced to decrease the effective diffusion coefficient, resulting in successful fractionations of proteins differing ~1% in size (i.e. (uni-muenchen.de)
  • Using this technique, we were able to obtain data for the proteome profile of full thickness human skin using on-line two-dimensional liquid chromatography, followed by ultra-high definition label-free mass spectrometry analysis (UDMS E ). We were able to identify in excess of 2000 proteins from a full thickness skin sample. (biomedcentral.com)
  • When comparing a gel-based fractionation technique and the on-line fractionation technique described here we found a 50 % increase in the number of proteins detected (data not shown). (biomedcentral.com)
Field-flow fraction5
  • It was the aim of this thesis to evaluate the applicability of asymmetrical flow field flow fractionation (AF4) in pharmaceutical protein analytics, to compare AF4 performance with established state-of-the-art methods and to reveal the effectivity of inherent AF4 characteristics in demanding analytical tasks. (uni-muenchen.de)
  • The Theoretical Section encompasses Chapter 2, wherein the family of field-flow fractionation techniques is introduced, as well as Chapter 3 (attending to protein aggregation) and Chapter 4, which provides an insight into multi-angle light scattering. (uni-muenchen.de)
  • Thermal field-flow fractionation and the advanced analysis of complex polymers. (sun.ac.za)
  • Novel characterization of decorative coatings using field-flow fractionation and a multi-detector approach. (sun.ac.za)
  • Field-flow fractionation of amphiphilic block copolymers. (sun.ac.za)
Permeation32
Ethanol3
  • Traditionally, the Cohn process incorporating cold ethanol fractionation has been used for albumin purification. (wikipedia.org)
  • Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. (sigmaaldrich.com)
  • Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. (sigmaaldrich.com)
Ammonium Sulphate1
  • The enzyme was purified 123.69 fold with a yield of 21.37% by ammonium sulphate fractionation, PEG-4000 precipitation, CM cellulose column chromatography and DEAE cellulose column chromatography. (scirp.org)
Silica gel4
  • Upgrade your purification performance with Silica Gel 60. (emdmillipore.com)
  • Our silica gels are available in many particle size ranges all derived from a single base silica gel, specifically produced only for adsorption and chromatography processes. (emdmillipore.com)
  • Chromatography columns are designed to be filled with a 'stationery phase' (usually silica gel) and the mixture to be separated is passed through the column with a solvent system. (rsc.org)
  • These prevent the need for chemists to handle fine, loose silica which can become airborne and present an inhalation risk when making silica gel. (rsc.org)
Cohn1
  • Developments were ongoing in the time period between when Cohn fractionation started being used, in 1946, and when chromatography started being used, in 1983. (wikipedia.org)
Electrophoresis1
Chromatographic methods1
  • Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. (scielo.br)
Isolation1
  • Gel-based protein isolation or fractionation techniques is a viable method for preparing samples prior to mass spectrometry analysis and one which we explored whilst developing the method presented in this paper. (biomedcentral.com)
Purification1
Protein1
  • This method is relatively quick, consists of less sample preparation steps and less protein (3 μg) compared with similar gel-based methods. (biomedcentral.com)
Cellulose1
  • This heterogeneity was still apparent in the presence of 6M urea and appeared to be unchanged following re‐chromatography on DEAE‐cellulose under the usual fractionation conditions. (elsevierpure.com)
Analytical1
Columns1
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
Molecular weights1
  • There was a discrepancy between the apparent molecular weights determined by gel‐filtration (70,000-150,000) and by zone sedimentation (64,000) as has been reported previously for other colony stimulating factors. (elsevierpure.com)
Laboratories1
Enzyme2
  • The molecular weight of the purified SOD was determined to be approximately 35.5 kDa by gel filtration and the enzyme was also shown to be composed of dimeric subunits on denaturing SDS-PAGE. (korea.ac.kr)
  • Results of subcellular fractionation of rat hepatocytes suggest that the enzyme is enriched in the microsomal fraction and, to a lesser extent, in the lysosomal fraction. (northwestern.edu)
Methods2
Polymers1
  • Gel permeationsize exclusion chromatography 5 chapter 2 gpcsec overview 6 polymers 6 size matters 6 how does gpcsec work 7 who uses gpcsec, what for and why 8 calibrations 8. (web.app)
Buffer1
Homogeneity1
Size3
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • Fractionation of ECM CSA revealed a slight size heterogeneity on gel‐filtration and on zone sedimentation in sucrose gradients. (elsevierpure.com)
  • A comparative study of AF4 with size exclusion chromatography (SE-HPLC) unveiled SE-HPLC to inhere higher recovery rates and AF4 to exhibit greater resolution. (uni-muenchen.de)
Sample1
  • System variables and parameters of fractionation were investigated, revealing that sample loads differing more than two orders of magnitude did not negatively affect data reproducibility. (uni-muenchen.de)
Analysis2
  • Cleanup by chromatography has been used extensively to separate the non- ortho and the mono- ortho CBs from the remaining congeners before quantitative analysis (Hess et al. (cdc.gov)
  • This is the system of high-performance liquid chromatography for proteome analysis. (nagasaki-u.ac.jp)
Development1
Method1
  • Standardized silica gels from MilliporeSigma are produced using a traditional method in the world's largest and most modern production plant for chromatography grade silica gels. (emdmillipore.com)
Exchange1
  • Controlled plasmin degradation at these sites converts Fbg into fragment D and fragment E. The individual fragments are isolated by salt fractionation, gel filtration and ion exchange chromatography. (cellsystems.eu)
Medicine1
  • The Institute of Transfusion Medicine in Skopje, North Macedonia is a plasma fractionation center in the Balkans. (wikipedia.org)
Products1
Find2
Process1