Found in mrna localization of specific rnas. Medical search. Frequent questions

Enzyme-catalyzed proximity labeling (PL), also known as proximity-based labeling, is a laboratory technique that labels biomolecules, usually proteins or RNA, proximal to a protein of interest. (wikipedia.org)
Labeling is achieved when the activated biotin reacts with nearby amines, such as the lysine sidechain amines found in proteins. (wikipedia.org)
A. thaliana PHIP1 and its homologs represent a novel class of plant-specific RNA-binding proteins that may play a unique role in the polarized mRNA transport to the vicinity of the cell plate. (nih.gov)
The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (antibodies-online.com)
These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. (antibodies-online.com)
This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. (antibodies-online.com)
Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. (elsevierpure.com)
Preliminary experiments suggest that by interacting with Argonaute2, PICK1 can relieve this block of protein synthesis and increase the production of specific proteins. (ukri.org)
MiRNAs silence target mRNAs via the RNA-induced silencing complex (RISC), of which Argonaute proteins (Ago) are the major subunit. (ukri.org)
RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. (uni-muenchen.de)
Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
Spatial segregation of proteins to neuronal axons arises in part from local translation of select mRNAs that are first transported into axons in ribonucleoprotein particles (RNPs), complexes containing mRNAs and RNA binding proteins. (biorxiv.org)
Fragile X granules (FXGs) are axonal RNPs containing the Fragile X related family of RNA binding proteins along with ribosomes and specific mRNAs. (biorxiv.org)
Restricting local translation to the appropriate cellular compartment at the correct developmental timepoint requires correct positioning of ribonucleoprotein particles (RNPs), complexes that contain mRNAs and the RNA binding proteins that control their translation. (biorxiv.org)
FXGs contain one or more of the Fragile X related (FXR) family of RNA binding proteins: FMRP (Fragile X mental retardation protein), FXR2P, and FXR1P. (biorxiv.org)
RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)
Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes, and proteins that were nucleolar in plants but nonnucleolar in human. (hud.ac.uk)
Seventy-two proteins were expressed as GFP fusions and 87% showed nucleolar or nucleolar-associated localization. (hud.ac.uk)
In the central dogma RNA acts as a simple intermediary between the DNA that carries the genetic information and the proteins that define the whole variety of biological processes in the cell. (biomedcentral.com)
During ribosomal RNA processing, ribosomal proteins are incorporated into the pre-ribosomal subunits to form the mature 40S and 60S subunits. (biomedcentral.com)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
The core is composed of 3 major (ie, lambda-1, lambda-2, sigma-2) and several minor proteins that surround 10 segments of double-stranded RNA. (medscape.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)

The introns containing these large expansions are transcribed and indeed in patients the GGGGCC-repeat expansion is detectable by in situ hybridization in nuclear RNA foci 5 . (nature.com)
We studied by in situ hybridization and RNA blots the pattern of 9E3 mRNA distribution in the wings of normal, wounded, and RSV-infected newly hatched chicks. (silverchair.com)
We have used in situ hybridization and immunocytochemistry to compare the nuclear localization of a specific nascent pre-mRNA and the essential non-snRNP splicing factor SC-35. (northwestern.edu)

While long non-coding RNAs (lncRNAs) are found abnormally expressed in cancer, how dysregulated lncRNAs contribute to carcinogenesis remains largely unknown. (cdc.gov)
Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. (biomedcentral.com)
The second group includes rRNAs and long non-coding RNAs (lncRNAs), which to date are very poorly functionally annotated. (biomedcentral.com)
In-depth analysis revealed that lncRNAs is an extensive and very heterogenic group from mRNA-like transcripts to circular RNAs. (biomedcentral.com)
According to the GENCODE project one third of all human lncRNAs genes are primate-specific [ 4 ]. (biomedcentral.com)
LncRNAs share a lot of common features with mRNAs, they are often capped, polyadenylated and undergo splicing. (biomedcentral.com)
Whereas 95% of all multi-exon mRNAs are alternatively spliced only 25% of lncRNAs undergo alternative splicing. (biomedcentral.com)
For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
Long non-coding RNAs (lncRNAs) are widely recognized as important regulators of gene expression. (mdpi.com)
Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)

The interaction of ABHD11-AS1 with SART3 promotes USP15 (ubiquitin specific peptidase 15) nuclear localization. (cdc.gov)
Nuclear localized USP15 interacts with pre-mRNA processing factor 19 (PRPF19) to increase CD44 RNA alternative splicing activating β-catenin and enhancing cancer stemness. (cdc.gov)
The family members consist of multiple functional domains, including a lysine-rich domain (KRD domain) that contains three nuclear localization motifs (KKKR/NK), two RNA recognition motifs (RRMs), and three CCHC-type zinc fingers. (nih.gov)
Its M9 domain acts as both a nuclear localization and nuclear export signal. (antibodies-online.com)
We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors. (elsevierpure.com)
Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
By using high-voltage electron microscopy, we have found that the c-fos path extends out and comes into direct contact with the nuclear envelope. (northwestern.edu)
We have also compared the localization of c-fos transcripts with the speckled nuclear regions that are enriched in snRNPs and the non-snRNP splicing factor SC-35. (northwestern.edu)
This study demonstrates a direct link between specific nascent RNA transcripts and nuclear speckles that are enriched in pre-mRNA splicing factors. (northwestern.edu)
Spector, David L. / Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors . (northwestern.edu)
Generally, lncRNA has a lower expression level, their expression is more tissue-specific, and the majority of lncRNA has nuclear localization. (biomedcentral.com)

Context and state of the art mRNA transport in general: Cytoplasmic mRNA transport and local translation are essential for many processes requiring symmetry breaking such as embryonic development, cell migration and neuronal differentiation (1). (europa.eu)
mRNAs are transported along microtubules (MTs) by different kinesins and cytoplasmic dynein and get anchored at the actin cortex, intermediate filaments or unknown structures (1, 3). (europa.eu)
Since the first discovery of cytoplasmic mRNA transport almost three decades ago, several essential questions could not be answered mostly due to the complexity of the in vivo situation and the approaches available. (europa.eu)

Our results represent the first genome-wide survey of mRNAs localized to a specific cytoskeletal component and suggest that microtubule localization of specific mRNAs is likely to function in mitotic regulation and mRNA segregation during cell division. (escholarship.org)
In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. (elsevierpure.com)
A comparison of their known functions has identified, besides a common role within protein folding, multiple roles for the cyclophilins within pre-mRNA splicing and cellular signalling, and within transcription and cell cycle regulation for the parvulins. (biomedcentral.com)
The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. (uni-muenchen.de)
The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. (idhinhibitor.com)
The 84th Cold Spring Harbor Symposium focused on RNA Control & Regulation and provided a current synthesis of the enormous progress in our understanding of RNA's influence on cells and organisms and how, when aberrant, its effects may contribute to the progression of disease. (cshlpress.com)
TF mRNA regulation by PARP-14 was selective, as tumor necrosis factor (TNF)α mRNA, which is also regulated by TTP, was not altered in PARP-14 deficient macrophages. (imperial.ac.uk)
When comparing the transcriptomes across developmental stages, we found an over-representation of genes involved in growth regulation in the early development stage in C. virgata . (frontiersin.org)

Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. (antibodies-online.com)
Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts. (bioscience.co.uk)
Prior to serum induction no c-fos RNA is detected, but these transcripts localize to two dots in the interphase nucleus after induction. (northwestern.edu)
LncRNA are found in all branches of life and the complexity of different organisms is well correlated with the amount and the diversity of these transcripts [ 3 ]. (biomedcentral.com)
Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis , respectively. (frontiersin.org)

Additionally, the 5′ and 3′ UTRs flanking the coding region regulate mRNA translation, half-life, and subcellular localization [2]. (researchgate.net)
The subcellular localization of EZH2 protein was predicted by using different predictors (CELLO, Euk-mPLoc, WoLF PSORT, and TargetP). (hindawi.com)

Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. (elsevierpure.com)
Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. (biomedcentral.com)
In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)

In addition to the cis -elements in its 3′UTR and trans -acting factor Staufen, posterior localization of oskar mRNA requires deposition of exon junction complex (EJC) in its first exon through splicing ( Hachet and Ephrussi, 2004 ). (frontiersin.org)
In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. (hud.ac.uk)

Another type of molecule, called micro RNA (miRNA) can bind to mRNA and stop the translation of mRNA into protein. (ukri.org)
We have found that a protein (called PICK1), which is known to be involved in synaptic plasticity over the timescale of hours, interacts with another protein (called Argonaute2), which is an important component of the cell machinery that promotes the association of miRNA with mRNA to block protein synthesis. (ukri.org)
The subcellular localisation of translational repression via miRNA and RISC in neurons is unknown, and the mechanisms for activity-dependent modulation are also unclear. (ukri.org)
In endometrial cancer only some of these cf-RNAs have been investigated, no studies have been performed on snoRNAs, piRNAs, snRNAs and tRNA, the most analyzed one is represented by cf-miRNA in plasma. (scientificarchives.com)
These findings open an immediate use of selected cf-miRNA as biomarker in liquid biopsy of EC and suggest further investigation of the other cf-RNAs to implement the knowledge in this fascinating field. (scientificarchives.com)
Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)

Shep harbors two highly conserved RNA recognition motifs (RRMs), and genetic evidence points to a functional relationship between its RNA-binding capability and insulator function. (nih.gov)

The goal of this study is to investigate the mechanism of Cr(VI)-induced lung carcinogenesis focusing on the role of the lncRNA ABHD11 antisense RNA 1 (tail to tail) (ABHD11-AS1). (cdc.gov)
It was found that the lncRNA ABHD11-AS1 expression levels are up-regulated in chronic Cr(VI) exposure-transformed human bronchial epithelial cells, chronically Cr(VI)-exposed mouse lung tissues, and human lung cancer cells as well. (cdc.gov)
Together, these findings indicate that lncRNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote cell malignant transformation and lung carcinogenesis. (cdc.gov)
Thousands of long non-coding RNA (lncRNA) genes are annotated in the human genome. (biomedcentral.com)
42% of lncRNA contains two exons in comparison with only 6% in mRNAs. (biomedcentral.com)
In addition, on average lncRNA are shorter than mRNA. (biomedcentral.com)
LncRNA is less conserved than mRNA, but the sequence conservation in lncRNA is not always correlated to their function. (biomedcentral.com)

Consistent with this finding, core components of EJC (eIF4AIII, Barentsz, Tsunagi, and Mago nashi) are critical for oskar mRNA localization ( Hachet and Ephrussi, 2001 , 2004 ). (frontiersin.org)
Previous Cold Spring Harbor Symposia have addressed many different aspects of RNA biology such as Mechanisms of Transcription (1998), The Ribosome (2001), Epigenetics (2004) and Regulatory RNAs (2006). (cshlpress.com)
2001. The effects of inhalation exposure to bromo dichloromethane on specific rat CYP isoenzymes. (cdc.gov)

Human ZAP inhibits many viruses, including HIV and coronaviruses, by binding to viral RNAs to promote their degradation and/or translation suppression. (bvsalud.org)

In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)

G-rich sequences have a propensity for forming highly stable quadruplex structures in both RNA and DNA termed G-quadruplexes. (nature.com)
Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
mRNA localisation requires primary sequences and secondary structures often localised in their 3'UTR (3). (europa.eu)
Their design of molecular probes that target the genomic sequences of specific pathogens can enable diagnosticians and clinical laboratories to spot extremely small amounts of viral and bacterial pathogens in patients' biological samples, as well as in the environment and wildlife. (darkdaily.com)
The National Human Genome Research Institute defines a "probe" in genetics as a "single-stranded sequence of DNA or RNA used to search for its complementary sequences in a sample genome . (darkdaily.com)
The (PCR) technique relies on primers that bind to nucleic acid sequences specific to an organism or group of organisms. (darkdaily.com)

To investigate how spindle-localized RNAs might influence mitosis, we comprehensively surveyed all messenger RNAs (mRNAs) that bound to microtubules during metaphase in both Xenopus laevis egg extracts and mitotic human cell extracts. (escholarship.org)
We identify conserved classes of mRNAs that are enriched on microtubules in both human and X. laevis. (escholarship.org)
Although many MT-mRNAs associate with polyribosomes, we find that active translation is not required for mRNA localization to mitotic microtubules. (escholarship.org)
While it is clear, that RNA distribution patterns in neurons are created by active transport processes of mRNPs along microtubules, the essential enzymatic activities required are not known. (europa.eu)
Before we can understand how overall distributions of thousands of mRNA are generated, we first need to understand how any specific mRNA can be transported along microtubules - a question unanswered since the first MT based mRNA transport was observed more than 2 decades ago. (europa.eu)

A large proportion of miRNAs are expressed in the brain, and most of these are found in neuronal dendrites associated with synapses. (ukri.org)
Shep is required for neuronal remodeling during development and is highly enriched in the CNS, perhaps serving to negatively regulate insulator function in these cell types to promote CNS-specific gene expression programs. (nih.gov)
In neurons, local translation is supported by a variety of RNPs that can differ in their prevalence, mRNA cargoes, and RNA binding protein composition depending upon developmental stage, neuronal cell type, and subcellular location. (biorxiv.org)
Neuronal mRNA transport occurs in packages of single to a few copies of mRNAs (8-11), mostly in a translationally repressed mode. (europa.eu)
An antibody prepared against a peptide sequence specific to the Aedes aegypti GPI-linked carbonic anhydrase labels plasma membranes of a number of cell types including neuronal cells and muscles. (silverchair.com)

After induction of long-term potentiation, CaMKIIa-mRNA is transported to distal parts of dendrites where its translation is locally regulated (12, 13). (europa.eu)

They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)

An intermediate between DNA and protein is called messenger RNA (mRNA), and neurons can transport mRNA to the parts of the neuron close to synapses and locally control the synthesis of a particular protein that is important for those synapses at a particular time. (ukri.org)
RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
We found FXGs in a conserved subset of neurons and circuits in the brains of every examined species that together include mammalian taxa separated by up to 160 million years of divergent evolution. (biorxiv.org)
mRNA transport in neurons: In neurons, thousands of mRNAs are transported into axons or dendrites by so far not identified transport mechanisms (6). (europa.eu)
C-E) Co-labeling VMHvl neurons for PR (anti--gal) and Cckar (mRNA) in PRPL/PL mice. (cdc.gov)

Mechanistically, it was found that ABHD11-AS1 directly binds SART3 (spliceosome associated factor 3, U4/U6 recycling protein). (cdc.gov)
It also binds specifically to Ran2 mRNA. (nih.gov)
We recently identified a novel RNA-binding protein, Isha, that binds to the su(Hw) mRNA and is required for gypsy insulator function. (nih.gov)

We focused our research on the nucleolar function that a specific member of EGFR family, the ErbB3 receptor, plays in glioblastoma, a tumor without effective therapies. (biomedcentral.com)

LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. (elsevierpure.com)

LO1-750 specifically identified necrotic core in ex vivo human coronary lesions.Injection of LO1-750 into high fat (HF) fed atherosclerotic Ldlr-/-mice led to specific focallocalization within the aortic arch and its branches, as detected by fluorescence moleculartomography (FMT) combined with micro-computed tomography (CT). (imperial.ac.uk)
Ex vivo confocalmicroscopy confirmed LO1-750 subendothelial localization of LO1-750 at sites ofatherosclerosis, in the vicinity of macrophages. (imperial.ac.uk)
In the rabbit, in vivo injected LO1-750 localization wassuccessfully imaged ex vivo in aortic lesions with a customised intra-arterial NIRF detectioncatheter. (imperial.ac.uk)
These results would not have been possible without critical recent innovations in the field, such as the development of safe and efficient materials for in vivo mRNA delivery and advanced protocols for the production of high quality mRNA. (researchgate.net)

Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. (imperial.ac.uk)

Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)

Using RNA immunoprecipitation followed by deep sequencing (RIP-seq), we made the striking finding that certain mRNAs, including that encoding Su(Hw) itself, associate stably with gypsy insulator complexes. (nih.gov)
Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)

The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. (bioscience.co.uk)

MicroRNAs (miRNAs) are small noncoding endogenous RNA molecules that repress target mRNAs through complementary binding in the message 3'-UTR. (ukri.org)

Since the early 1990s, patients with both CMT1 and CMT2, while often clinically similar, were found to be genetically heterogeneous. (medscape.com)

Ribosomal RNA is synthesised as precursor 47S pre-rRNA which is subjected to specific and highly regulated processing steps that allow to mature 18S, 5.6S and 28S RNAs. (biomedcentral.com)

When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. (bioscience.co.uk)

Nanos family is characterized by two specific Cys-Cys-His-Cys zinc finger motifs at the carboxy-terminal region which present a regular spacing between the Cys and the His residues. (nature.com)

Active mitotic translation occurs on X. laevis meiotic spindles, and a subset of microtubule-bound mRNAs (MT-mRNAs) associate with polyribosomes. (escholarship.org)

This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)
Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)
The CDC's move came the day after the US Food and Drug Administration (FDA) approved Moderna's and Pfizer-BioNTech's updated COVID-19 messenger RNA (mRNA) vaccines for people 12 years or older and authorized the shots for emergency use in children 6 months through 11 years of age. (cdc.gov)
2, 3, 4, 5] The WT1 gene contains 10 exons that produce 4 different messenger RNAs (mRNAs) as a result of 2 alternative splicing sites in exons 5 and 9 that, in turn, encode 4 different isoforms of the WT1 protein. (medscape.com)

In 2019, BioID has been tethered to the beta-actin mRNA transcript to study its localization dynamics. (wikipedia.org)
Multiple transcript variants encoding different isoforms have been found for this gene. (idhinhibitor.com)
Based on the length of the transcript non-coding RNAs are divided into two groups: short and long non-coding RNAs. (biomedcentral.com)

In 2019, Alice Ting and the Ting lab have used APEX to identify RNA localized to specific cellular compartments. (wikipedia.org)
Transcriptomic analysis of ZAP-isoform-specific knockout cells revealed uncharacterized host mRNAs targeted by ZAPL/S with broad cellular functions such as unfolded protein response (UPR), epithelial-mesenchymal transition (EMT), and innate immunity. (bvsalud.org)
The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. (hud.ac.uk)

G-quadruplexes have been shown to be involved in a range of processes including telomere stability and RNA transcription, splicing, translation and transport. (nature.com)
Tumor suppressors, oncogenes and alternatively deregulated upstream signalling pathways can directly influence the RNA polymerase I activity inducing hyper activation of rRNA transcription in cancer cells. (biomedcentral.com)
The virion core contains several enzymes needed for transcription and capping of viral RNA. (medscape.com)

We find that RBPs exhibit dynamic temporal and fiber-type specific expression patterns in mRNA-Seq data and display muscle-specific phenotypes. (uni-muenchen.de)
These analyses of the relationships between patient survival and expression patterns obtained from an open mRNA database in cancer patients indicate that ORs may be cancer biomarkers and therapeutic targets. (bmbreports.org)
We showed that the method is capable of measuring high RNA quality in tissue areas of both high and low cell density and that the spatial RNA integrity patterns are reflected in spatial transcriptomics data. (kth.se)

This optimism is built on recently published studies demonstrating the efficacy of mRNA vaccines in combatting several types of cancer and infectious pathogens where conventional vaccine platforms may fail to induce protective immune responses. (researchgate.net)
This review summarizes the most important developments in mRNA vaccines from the past few years and discusses the challenges and future directions for the field. (researchgate.net)
The mRNA vaccines can encode multiple antigens, strengthening the immune response against pathogens and enabling the targeting of multiple microbial variants [19] . (researchgate.net)
For example, 'Two mRNA vaccines developed by Pfizer-BioNTech and Moderna are effective against the coronavirus. (researchgate.net)
mRNA COVID vaccines saved lives and won a Nobel - what's next for the technology? (cdc.gov)
From the paper: 'In just three short years, mRNA vaccines have saved millions of lives, achieved household recognition and, as of this week, become the subject of a Nobel Prize. (cdc.gov)
Vaccines based on mRNA rose to fame not only for their safety and efficacy, but also for the speed with which they were developed and rolled out during the COVID-19 pandemic. (cdc.gov)

The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets. (scialert.net)

We recently found that Shep can antagonize enhancer-promoter looping of one of its downstream transcriptional targets in a temporal manner. (nih.gov)
Tumor suppressors such as retinoblastoma (pRB) and p53 negatively regulate RNA polymerase I and interfere with the assembly of transcriptional machinery on the rDNA promoter. (biomedcentral.com)

The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. (antibodies-online.com)

Dendritic localisation of CaMKIIa-mRNA requires its 3'UTR harbouring binding sites for RBPs as FMRP and Staufen2, which are required for its correct localisation. (europa.eu)

Long noncoding RNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote lung carcinogenesis. (cdc.gov)

Proximity labeling has also been used to find interaction partners of heterodimeric protein phosphatases, of the miRISC (microRNA-induced silencing complex) protein Ago2, and of ribonucleoproteins. (wikipedia.org)
In this context, during any sort of stress condition the alternative reading frame protein p14ARF subtracts MDM2, an E3 ubiquitin ligase, from the interaction with p53 in manner to free and stabilise p53 level in the nucleolus of the cell where it inhibits RNA polymerase I activity [ 3 ]. (biomedcentral.com)

RNA localization is of critical importance in many fundamental cell biological and developmental processes by regulating the spatial control of gene expression. (escholarship.org)

In addition, OR expression pattern in various cancers were analyzed based on RNA-sequencing data reported in the Cancer Genome Atlas (TCGA), and the associations between patient survival outcomes and OR levels were analyzed to determine the clinical relevance and significances of OR expression in tumors. (bmbreports.org)
Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)
In contrast, we found huge variabilities investigating several malign tissue types ranging from homogenous (pediatric medulloblastoma) to highly variable genomes (ductal breast cancer and glioblastoma). (kth.se)
Malentacchi F, Sorbi F, Cipriani N, Sgromo C, Antonuzzo L, Pillozzi S. Circulating Cell-Free RNA: A New Perspective for Endometrial Cancer. (scientificarchives.com)
Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)
We focus on the role of HPV in the increased incidence of oropharyngeal squamous cell carcinoma (OSCC), the head and neck cancer in which HPV is most commonly found ( 4 ). (cdc.gov)

Similar elevation of mRNA occurs immediately adjacent to RSV tumors but, surprisingly, the tumor tissue itself shows no detectable levels of this message. (silverchair.com)
Microglia cells have been implicated to play an important role in the tumor microenvironment, and we found spatial co-localization of microglia and epithelial-to-mesenchymal transition (EMT) signatures in our patient cohort. (kth.se)
Furthermore, we found homogenous and recurrent iCNV profiles in the high-grade tumors of relapse patients and identified expression of gene SPP1 in the tumor stroma as a potential prognostic mRNA marker in pediatric brain tumor relapse patients. (kth.se)

Expression of untranslatable versions of these mRNAs alters insulator body localization and promotes insulator activity. (nih.gov)

A good example is the CaMKIIa-mRNA, which encodes the a-subunit of the Ca2+-calmodulin kinase II. (europa.eu)

We further show that splicing mediated by SF1 and Hrb87F is necessary for Z-disc stability and proper myofibril development, and strong knockdown of either gene results in impaired localization of kettin to the Z-disc. (uni-muenchen.de)
Despite this, the mRNAs have concerns about their stability and immuno-stimulation, therefore, researchers and companies have concentrated their work to address this issue [2]. (researchgate.net)

Our Custom LNA mRNA Detection Probes are designed with optimal LNA positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. (qiagen.com)
Our user-friendly online design tool lets you easily design highly sensitive LNA probes for your specific mRNA sequence of interest. (qiagen.com)

Insulators are required for the formation of topologically associating domains (TADs), and on a local scale, insulators enforce the strict specific and temporal expression of loci with complex enhancer and/or promoter configuration. (nih.gov)
Localization of 9E3/CEF-4 in avian tissues: expression is absent in Rous sarcoma virus-induced tumors but is stimulated by injury. (silverchair.com)
Cells explanted from the tumors and grown in culture also show no expression of 9E3, in marked contrast to the very high level found in similarly cultured RSV-transformed CEFs. (silverchair.com)
In efforts to identify pharmacological agents that target this pathway, we found that fisetin, a natural flavonoid, is an ULK1 inhibitor that decreases type I IFN-induced dACE2 expression. (bvsalud.org)
Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
To analyze the regulatory mechanism involved in the high growth potential of C. virgata , we performed RNA-seq-based transcriptome analysis and illustrated a comprehensive gene expression map of the species. (frontiersin.org)

Understanding the importance of local translation for a particular circuit requires not only identifying axonal RNPs and their mRNA cargoes, but also whether these RNPs are broadly conserved or restricted to only a few species. (biorxiv.org)

We established that endogenous ZAPL and ZAPS localize to membrane compartments and cytosol, respectively, and that the differential localization correlates with their target-RNA specificity. (bvsalud.org)
Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. (bioscience.co.uk)

Synapses can change their strength (known as 'synaptic plasticity') by altering the number of receptors found on the surface of the neuron in the synapse, and also by changing the size and shape of the dendritic spine that houses the synapse. (ukri.org)

The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
Important recent specific progress was brought to my attention by Dr. Victoria Lunyak , a lab director at the Buck Institute who presented on her recent work at the Cell signaling, inflammation and aging symposium in Las Vegas last week, where I was also a presenter. (anti-agingfirewalls.com)
IF we could find a way to rejuvenate adult stem cells in their niches, then the stem cell supply chain could possibly be transformed from being a once-through-in-life process to a continuing closed-loop process. (anti-agingfirewalls.com)
To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)

We found that the splicing factors hnRNPA1/A2, PTBP1/2, and U1-snRNP inhibit ZAPS production and demonstrated the feasibility to modulate the ZAPL/S balance by splice-switching antisense oligonucleotides in human cells. (bvsalud.org)

Anatomy33

Organisms20

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Psychiatry and Psychology1

Phenomena and Processes80

Disciplines and Occupations3

Information Science4

Proteins31

Situ hybridization3

LncRNAs11

Nuclear12

Cytoplasmic3

Regulation9

Transcripts5

Subcellular localization2

Assay3

Exon junction complex2

MiRNA7

Highly conserved1

LncRNA7

20013

Degradation1

Genome-wide1

Sequences6

Microtubules5

Neuronal5

Distal1

Regulate gene expression1

Neurons6

Binds3

Nucleolar1

Structures1

Vivo4

Mechanisms2

Blots1

Immunoprecipitation2

Quantification1

MiRNAs1

Heterogeneous1

Precursor1

Molecules1

Motifs1

Bound1

Messenger4

Transcript3

Cellular3

Transcription3

Patterns3

Vaccines7

Roles1

Transcriptional2

Particles1

FMRP1

Regulates1

Interaction2

Control of gene expression1

Cancer6

Tumor3

Promotes1

Subunit1

Stability2

Sequence2

Expression6

Translation1

Target2

Synaptic plasticity1

Cell5

Modulate1