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  • intracellular
  • The intracellular localization and specific organelle association of mRNA may reflect essential functions, stages, and stability of mRNA. (spie.org)
  • Analysis of PQBP1 complexes revealed six novel interacting proteins, namely the RNA-binding proteins KSRP, SFPQ/PSF, DDX1 and Caprin-1, and two subunits of the intracellular transport-related dynactin complex, p150(Glued) and p27. (mpg.de)
  • To gain a deeper understanding of the hitherto poorly understood nature of miRNA:PB interactions, to more accurately quantify the distribution of miRNAs within PBs, and to unravel the relative contribution of PBs in miRNA-mediated gene silencing, we integrated our recently developed iSHiRLoC1,2,3 (intracellular Single molecule High-Resolution Localization and Counting) technique with systems biology modeling. (utoronto.ca)
  • synaptic
  • have been interested in identifying the population of localized mRNAs, because these are likely to encode proteins that function to produce local changes in synaptic efficacy and structure. (jneurosci.org)
  • Whereas GluRIIA and GluRIIB are redundant for viability, it was shown recently that GluRIII is essential for both the synaptic localization of GluRIIA and GluRIIB and the viability of Drosophila. (jove.com)
  • nanos
  • In Drosophila , localization of bicoid and nanos RNA is required for establishment of the anterior/posterior axis of the embryo (for review see St Johnston and Nüsslein-Volhard, 1992 ). (rupress.org)
  • nascent
  • Görlich and Mattaj, 1996 ) and for targeting mRNAs encoding secretory and membrane proteins ( Blobel and Dobberstein, 1975 ) to the endoplasmic reticulum (ER) 1 via the nascent polypeptide. (rupress.org)
  • mechanism
  • Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. (abcam.com)
  • We have found that homology-dependent repair (HDR) is a highly efficient mechanism with two important characteristics: HDR requires templates with short (35bp) homology arms and is prone to template switching. (hhmi.org)
  • Both proteins selectively upregulated E1A-10S mRNA accumulation in transfection experiments, by a mechanism independent of the tiny RS-repeat. (diva-portal.org)
  • Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. (fiocruz.br)
  • pathways
  • Thus, localization of coding RNA is RLR dependent, and localization of noncoding RNA is RLR independent, suggesting that they are localized by different pathways. (rupress.org)
  • Our single molecule systems biology approach opens up an avenue toward better understanding the mechanistic underpinnings of RNA silencing pathways in healthy and cancer-diseased cells. (utoronto.ca)
  • Cell Differentiation
  • Although knockout mutants of PDF2 and ATML1 , another L1-specific HD-GL2 class gene sharing the highest homology with PDF2 , display normal shoot development, the double mutant results in severe defects in shoot epidermal cell differentiation. (biologists.org)
  • molecule
  • In the central dogma of molecular biology, messenger RNA (mRNA) is the molecule linking the genetic code, DNA, with the cellular work force, proteins. (mdpi.com)
  • discrete
  • The discrete pattern of hybridization for VIP/PHI mRNA indicates that these two peptides are synthetized in rat retina and SCN and suggests that they may play a key role in the photic entrainment to the SCN regulating various circadian rhythms. (springer.com)
  • Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus. (rupress.org)
  • In cells from sea urchin to human, nuclear poly(A) RNA was found concentrated primarily within several discrete "transcript domains" which often surrounded nucleoli. (rupress.org)
  • lncRNAs
  • In addition to these key players, many other RNA species have been identified such as long non-coding RNAs (lncRNAs), micro RNAs (miRNAs) or even enzymatic RNAs like RNase P [ 1 ]. (mdpi.com)
  • The RNA composition of the subcellular fractions differs significantly from each other, but lncRNAs are found in all locations. (biomedcentral.com)
  • tissue
  • Furthermore, we use our internal standard for the quantification of modified nucleosides in mouse tissue messenger RNA (mRNA), where we find different modification profiles in liver and brain tissue. (mdpi.com)
  • Transcriptome analyses revealed that the expression of ~5-10% of genes exhibits circadian rhythmicity at steady-state mRNA levels in any given organism or tissue ( Duffield, 2003 ). (biologists.org)
  • elegans
  • Lublin, A.L. and Evans, T.C. (2007) The RNA-binding proteins PUF-5 and PUF-6/7 reveal multiple systems for maternal mRNA regulation during C. elegans oogenesis Developmental Biology 303, 635-649. (ucdenver.edu)
  • In C. elegans , apoptosis occurring through a specific `physiological' pathway normally claims about half of all developing oocytes. (biologists.org)
  • 1991
  • Denis P, Dussaillant M, Nordmann JP, Elena PP, Saraux H, Rostène W (1991) Autoradiographic characterization and localization of vasoactive intestinal peptide binding sites in albino rat and rabbit eyes. (springer.com)
  • precursor
  • Dussaillant M, Sarrieau A, Gozes I, Berod A, Rostène W (1992) Distribution of cells expressing vasoactive intestinal peptide/ peptide histidine isoleucine-amide precursor messenger RNA in the rat brain. (springer.com)
  • situ hybridization
  • The localization of the messenger RNA (mRNA) encoding vasocative intestinal peptide/peptide histidine isoleucine (VIP/PHI) in the rat eye was studied by in situ hybridization histochemistry using a synthetic 35 "S-labeled oligodeoxyribonucleotide. (springer.com)
  • Their exclusive localization within the epithelial cells was verified by in situ hybridization. (nih.gov)
  • Dendritically localized mRNAs were initially identified fortuitously in the course of in situ hybridization (ISH) analyses. (jneurosci.org)
  • These experiments suggest that there is a large population of dendritically localized mRNAs, although wide acceptance of the dendritic localization of these mRNAs has been delayed by the lack of in situ hybridization to demonstrate that the mRNAs are indeed present in dendrites. (jneurosci.org)
  • translation
  • Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. (abcam.com)
  • In particular, evidence shows that defective mRNA transport and translation are implicated. (frontiersin.org)
  • Here, we provide a review of what is currently known about altered mRNA translation in ALS and how this impacts on the ability of affected cells to cope with proteotoxic stress. (frontiersin.org)
  • To understand the relationship between RNA localization and translation, it is necessary to visualize the movement of the RNA in real time. (ibiology.org)
  • miRNAs
  • We found that only a small fraction (~10%) of miRNAs resides within PBs, predominantly as monomeric or dimeric micro-ribonucleoprotein complexes (miRNPs). (utoronto.ca)