Formation of g quadruplexes g quadruplexes. Medical search. Frequent questions

This book provides current research on G-quadruplex structures, their formation and role in biology. (novapublishers.com)
In molecular biology, G-quadruplex secondary structures (G4) are formed in nucleic acids by sequences that are rich in guanine. (wikipedia.org)
G-quadruplex structures can be computationally predicted from DNA or RNA sequence motifs, but their actual structures can be quite varied within and between the motifs, which can number over 100,000 per genome. (wikipedia.org)
With the abundance of G-quadruplexes in vivo, these structures hold a biologically relevant role through interactions with the promoter regions of oncogenes and the telomeric regions of DNA strands. (wikipedia.org)
Current research consists of identifying the biological function of these G-Quadruplex structures for specific oncogenes and discovering effective therapeutic treatments for cancer based on interactions with G-quadruplexes. (wikipedia.org)
Early evidence for the formation of G-quadruplexes in vivo in cells was established by isolating them from cells, and later by the observation that specific DNA helicases could be identified where small molecules specific for these DNA structures accumulated in cells. (wikipedia.org)
These structures, formed from two separate G-rich strands, are termed bimolecular quadruplexes. (wikipedia.org)
Finally, sequences which contain four distinct runs of guanine bases can form stable quadruplex structures by themselves, and a quadruplex formed entirely from a single strand is called an intramolecular quadruplex. (wikipedia.org)
G-rich sequences have a propensity for forming highly stable quadruplex structures in both RNA and DNA termed G-quadruplexes. (nature.com)
Guanine (G)-quadruplexes are highly stable nucleic acid secondary structures formed from short tracts of G-rich sequence associating together. (nature.com)
G-quadruplexes are higher-order nucleic acid structures formed by tetrads of guanine bases (G-tetrads) through non-canonical base interactions. (herts.ac.uk)
rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. (ox.ac.uk)
Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)
It has been indeed demonstrated that the involvement of G-rich sequences in duplex architecture is compatible with their folding into quadruplex structures, thanks to the breathing of duplex DNA [ 27 ]. (hindawi.com)
G-quadruplexes (G4s) are four-stranded nucleic acid structures with closely spaced guanine bases forming square planar shapes in genomes that were implicated in genome stability and cellular regulation. (usda.gov)
G-quadruplexes (G4s) are four-stranded nucleic acid structures with closely spaced guanine bases forming square planar shapes. (usda.gov)
Aberrant formation of G4 structures has been associated with genomic instability, functional similarities of the G4s in transcription, replication, and translation have been observed in related species. (usda.gov)
We also find that rG4 formation is associated with its stability, Cytosine (C)-content, and the stability of alternative RNA structures. (nih.gov)
Quadruple helix structures, called DNA G-quadruplexes (G4s), have previously been detected in cells. (barfblog.com)
G-quadruplexes are secondary semi-stable folded structures found in our DNA and RNA which tend to assemble around guanine-rich sequences in the presence of cation molecules like potassium. (anti-agingfirewalls.com)
The formation (folding) of DNA G-quadruplexes and the unfolding of G-quadruplex structures to allow telomerease to work is highly evolved. (anti-agingfirewalls.com)
The negative supercoiling of DNA can induce sequence-dependent conformational changes that give rise to local DNA structures and alternative DNA conformations such as cruciforms, A-DNA, left-handed DNA (Z-DNA), triplexes, four-stranded DNA (quadruplexes) and others [ 2 , 3 ]( ref )" But we don't really get into most of those other types here. (anti-agingfirewalls.com)
The outcomes - concludes Bozzoni - highlight how important the RNA's complex structures, such as G- quadruplex, are and, by identifying this new regulation mechanism, they cast new light on the non-coding RNA's functional repertoire, the "dark side" of our cell transcriptions . (uniroma1.it)
The most well-studied form of stable secondary DNA structures is the G quadruplex (G4) structure. (brc.hu)
G-quadruplexes are nucleic acid secondary structures that form within guanine-rich DNA or RNA sequences. (bgu.ac.il)
On the other hand, it has been hypothesised that the formation of quadruplex DNA structures in the promoter region of some oncogenes plays an important role in regulating the transcription of the corresponding gene. (eurekaselect.com)
There has recently been a huge increase in interest in the formation of stable G-quadruplex structures in mRNAs and their functional significance. (silverchair.com)
However, it has now been shown that single stranded DNA can fold into hairpin, triplex, i-motif and G-quadruplex structures. (univ-grenoble-alpes.fr)
The more interesting G-quadruplex DNA structures comprise four strands of stacked guanine (G)-tetrads formed by the coplanar arrangement of four guanines, held together by Hoogsteen bonds. (univ-grenoble-alpes.fr)
The DNA sequences with potential to form G-quadruplex structures are found at the chromosomal extremities (i.e. the telomeres) and also at the intra-chromosomal region (i.e. oncogenic promoters) in several important oncogenes. (univ-grenoble-alpes.fr)
The formation of G-quadruplex structures is considered to have important consequences at the cellular level and such structures have been evoked in the control of expression of certain genes involved in carcinogenesis (c-myc, c-kit, K-ras etc.) as well as in the perturbation of telomeric organization. (univ-grenoble-alpes.fr)
Therefore, G-quadruplex structures are an important target for drug design and development and there is a huge interest in design and development of small mols. (univ-grenoble-alpes.fr)
RNA G-quadruplexes (RG4s) are four-stranded structures known to control mRNA translation of cancer relevant genes. (unitn.it)
Here, we have used a model system to provide evidence about the formation of G-quadruplex structures involving telomeric DNA and RNA sequences that have the potential to provide a protective capping structure for telomere ends. (elsevierpure.com)
Guanine quadruplexes are nucleic acid structures built ions in slow exchange with bulk ions. (lu.se)

The importance of discovering G-quadruplex structure was described through the statement, "If G-quadruplexes form so readily in vitro, Nature will have found a way of using them in vivo" - Aaron Klug, Nobel Prize Winner in Chemistry (1982). (wikipedia.org)

Thus, over the past decade telomeric G-quadruplex DNA has been thoroughly studied with an initial focus on the possible interferences with telomerase activity [ 25 ]. (hindawi.com)
Anticancer activity for targeting telomeric G-quadruplex and antiangiogenesis of a novel Ru(II)-Se complex. (telomerescience.com)

Interest in in vivo function of G-quadruplexes surged after large scale genome-wide analysis showed the prevalence of potential G-quadruplex (pG4)-forming sequences within gene promoters of human, chimpanzee, mouse, and rat - presented in the First International G-quadruplex Meeting held in April 2007 in Louisville, Kentucky. (wikipedia.org)
The length of the nucleic acid sequences involved in tetrad formation determines how the quadruplex folds. (wikipedia.org)
Short sequences, consisting of only a single contiguous run of three or more guanine bases, require four individual strands to form a quadruplex. (wikipedia.org)
Longer sequences, which contain two contiguous runs of three or more guanine bases, where the guanine regions are separated by one or more bases, only require two such sequences to provide enough guanine bases to form a quadruplex. (wikipedia.org)
By computational analysis, it was found that FXYD1 can fold into G-quadruplex and multiple sequence alignment of ortholog FXYD1 sequences indicated that G-quadruplex-forming potential is conserved in evolution, hinting at a potential regulatory mechanism of FXYD1 expression. (herts.ac.uk)
The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)
Nevertheless, we have to keep in mind that these G-quadruplex-forming sequences although transiently single-stranded are a priori not as easily accessible as the telomeric sequences, due to the presence of the transcription machinery [ 28 ]. (hindawi.com)
Recently, these intrachromosomal G4 sequences have been shown to play an important function in the regulation of transcription and translation, in the firing of replication origins, and in the formation of recombination hot spots. (brc.hu)
Thus, it is highly desirable to have a computational method to predict G-quadruplex formation of new DNA sequences or whole genomes. (bgu.ac.il)
Here, we present a new method, called G4detector, to predict G-quadruplexes from DNA sequences based on multi-kernel convolutional neural networks. (bgu.ac.il)
In this study, using model human telomeric DNA and RNA sequences, we demonstrated that human telomeric RNA and DNA oligonucleotides form a DNA-RNA G-quadruplex. (elsevierpure.com)

For intramolecular quadruplexes, this means that any loop regions present must be of the propeller type, positioned to the sides of the quadruplex. (wikipedia.org)
We have also compared the melting of inter- and intramolecular quadruplexes. (soton.ac.uk)

Macrocyclic scaffolds are particularly attractive for designing selective G-quadruplex ligands essentially because, on one hand, they show a poor affinity for the "standard" B-DNA conformation and, on the other hand, they fit nicely with the external G-quartets of quadruplexes. (hindawi.com)
Among them, macrocycles rapidly became popular for recognition of quadruplexes motivated by the fact that telomestatin is one of the most active known G-quadruplex ligands [ 33 ]. (hindawi.com)
This review aims to discuss the different types of ligands that have been recently developed as quadruplex DNA stabilisers. (eurekaselect.com)
A large no. of so-called G-quadruplex ligands, displaying varying degrees of affinity and more importantly selectivity (i.e. the ability to interact only with quadruplex-DNA and not duplex-DNA), have been reported. (univ-grenoble-alpes.fr)
This tutorial review provides an overview of G-quadruplex ligands and biophys. (univ-grenoble-alpes.fr)
Templated Formation of Discrete RNA and DNA:RNA Hybrid G-Quadruplexes and Their Interactions with Targeting Ligands. (univ-grenoble-alpes.fr)

Chapter One summarizes the structural features of quadruplexes built by sequence extensions and site-specifically incorporated modified building blocks that have been established to induce the formation of stable, majority single G-quadruplex folding topologies, primarily from the human telomere repeat DNAs. (novapublishers.com)
Here we show using NMR and CD spectroscopy that the C9orf72 hexanucleotide expansion can form a stable G-quadruplex, which has profound implications for disease mechanism in ALS and FTD. (nature.com)
The QPA reaction is based on formation of "an unusually stable monomolecular quadruplex" by a guanine rich sequence constructed with fluorescent nucleotides, Kankia said. (genomeweb.com)

Interestingly, several classes of small molecules have been developed that efficiently target G-quadruplex DNA [ 29 - 32 ]. (hindawi.com)
Interestingly, G-quadruplex DNA associates with mtDNA deletions formation in human diseases. (openaire.eu)

The results provided clear evidence of G-quadruplex formation in vitro and together with evolutionary conservation point to potential role in regulating expression of FXYD1 possibly through alternative splicing and thus regulate indirectly the activity of Na+/K+-ATPase. (herts.ac.uk)
Access to efficient and robust in vitro assays is needed to effectively monitor and quantify the G-quadruplex DNA/ligand interactions. (univ-grenoble-alpes.fr)
RG4 formation is pervasive in vitro but not in cellulo, indicating the existence of poorly characterized molecular machinery that remodels RG4s and maintains them unfolded. (unitn.it)

FXYD1 pre-mRNA was investigated for its ability to form G-quadruplexes. (herts.ac.uk)

Many critical aging-related processes like the telomere/telomerase story can't be fully told without considering G-quadruplexes. (anti-agingfirewalls.com)
It has been shown that folding singlestranded telomeric DNA into a quadruplex structure inhibits telomerase (an enzyme overexpressed in 85-90% of cancer cells). (eurekaselect.com)
It has been shown that the formation of quadruplexes inhibits the telomere extension by the telomerase enzyme, which is up-regulated in cancer cells. (univ-grenoble-alpes.fr)
The G-quadruplex DNA-binding properties of the complex and its selenium ligand (Phen-Se) were evaluated by thermal denaturation study, polymerase chain reaction (PCR) stop assay, and telomerase repeat amplification protocol (TRAP). (telomerescience.com)
The results showed that the obtained complex could induce and stabilize G-quadruplex structure as well as exhibit potent inhibitory activity against telomerase. (telomerescience.com)

Chapter Four describes the G-quadruplex induced fluorescent signals that have been employed for the study of their folding and unfolding mechanism and thereby visualisation of their formation and structure. (novapublishers.com)
Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad or G-quartet), and two or more guanine tetrads (from G-tracts, continuous runs of guanine) can stack on top of each other to form a G-quadruplex. (wikipedia.org)
The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. (wikipedia.org)
We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability. (ox.ac.uk)
Indeed, given that four guanine residues can self-associate in a planar arrangement through a Hoogsteen-type hydrogen-bonding network [ 4 ] to form a structure called G-quartet, G-rich strands can adopt a peculiar three-dimensional arrangement called G-quadruplex DNA [ 5 - 9 ] resulting from the stacking of several contiguous G-quartets (Figure 1 ). (hindawi.com)
Numerous studies currently suggest that quadruplex formation in this overhang alters the structure and function of telomeres, inducing a damage response and rapid apoptosis in particular in cancer cells [ 14 - 24 ]. (hindawi.com)
Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K+ solution. (ntu.edu.sg)
Inside this RNA - says Irene Bozzoni, team coordinator - there is a G-quadruplex structure, a sort of knot that needs to be untangled by an enzyme, DHX36, so that its inner information can be efficiently translated and converted into proteins. (uniroma1.it)
The researchers have demonstrated how Inc-SMaRT, by connecting itself with the G-quadruplex structure of the messenger RNA MLX-g, can decrease the corresponding protein's levels and accurately organise the single stages leading to the right muscle differentiation . (uniroma1.it)
The consensus sequence which can potentially form a G quadruplex structure is (G3-5-N1-7)4. (brc.hu)
The formation of the nascent G-quadruplex structure is then detected by the G-quadruplex-selective iridium(iii) complex with an enhanced luminescent response. (edu.hk)
In an attempt to find a common shared sequence among the aptamers studied, it was found that some of them contain contiguous GG, and moreover, they can adopt a quadruplex structure. (unina.it)
These aptamers, that fold in a G-quadruplex structure and are dimers, have shown a specific interaction with the native form of PrP. (unina.it)
hDNA2 localizes to mitochondria and has the biochemical properties to process four-stranded DNA structure (G-quadruplexes). (openaire.eu)
23Na NMR findings but contrary to the only crystal structure of this quadruplex. (lu.se)
A contributions from external and internal ions and from unique feature of the quadruplex structure is the presence different classes of internal ions. (lu.se)
The processes of RNA tertiary structure formation. (bvsalud.org)

Chapter Two discusses the G-quadruplexes and i-motifs as scaffolds for molecular engineering of DNA aptamers. (novapublishers.com)
These insights allow us to better understand the formation of various structural elements in G-quadruplexes and should be useful for designing and manipulating G-quadruplex scaffolds with desired properties. (ntu.edu.sg)

and discusses the future applications of G-quadruplex interacting small molecules, including the development as potential drug candidates. (novapublishers.com)
Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)
Consequently, there is great current interest in developing small molecules that can bind selectively to quadruplex DNA and in doing so could act as anticancer drugs. (eurekaselect.com)

G-quadruplex formation can affect chromatin architecture and gene regulation and has been associated with genomic instability, genetic diseases and cancer progression. (bgu.ac.il)

The existence of G-quadruplex DNA is also heavily implied in the promoter region of genes and oncogenes, and is thus assumed to play an important regulatory role in their transcription [ 26 ]. (hindawi.com)

The VNTR sequence, which contains the potential G-quadruplex-forming sequence CGC(GGGGCGGGG)n, is involved in the downregulation of α-globin expression. (jascoinc.com)
Here, we performed volumetric analyses of formation of the complex between an oligonucleotide with the sequence of human telomeric DNA (h-telo) and hemin . (bvsalud.org)
In the presence of PTK7, the specific binding of the sgc8 aptamer sequence triggers a structural transition and releases the G-quadruplex-forming sequence. (edu.hk)

ABSTRACT: The structural stability of guanine quadruplexes depends critically on an unusual configuration of dehydrated Na+ or K+ ions, closely spaced along the central axis of the quadruplex. (lu.se)

The recognition of G-quadruplexes in mRNAs, their transport as repressed complexes and the control of their translation at their subcellular destinations involves a diversity of proteins, including those associated with disease pathologies. (silverchair.com)
A series of luminescent iridium(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. (edu.hk)

Influence of the SPR Experimental Conditions on the G-Quadruplex DNA Recognition by Porphyrin Derivatives. (univ-grenoble-alpes.fr)

Špacková N., Berger I., Šponer J.: Nanosecond molecular dynamics simulations of parallel and antiparallel guanine quadruplex molecules. (learned.cz)

Such magnetic relaxation dispersion (MRD1) structural polymorphism of quadruplexes ( 2, 5, 6 ), but little measurements have been used to study ion binding (via 23Na is known about the energetics and kinetics of ion binding. (lu.se)

In 2006, the prevalence of G-quadruplexes within gene promoters of several bacterial genomes was reported predicting G-quadruplex-mediated gene regulation. (wikipedia.org)
this reveals predominant R-loop formation near gene promoters with strong G/C skew and propensity to form G-quadruplex in non-template DNA, corroborating with all biochemically established properties of R-loops. (escholarship.org)

Methods for investigating G-quadruplex DNA/ligand interactions. (univ-grenoble-alpes.fr)

Such a quadruplex is described as tetramolecular, reflecting the requirement of four separate strands. (wikipedia.org)
8-Amino guanine accelerates tetramolecular G-quadruplex formation. (irbbarcelona.org)

And, G-quadruplex formation on single stranded DNA is one of the ways that the telomeric DNA is protected from oxidative stress and from triggering the DNA-damage response (DDR), which causes cellular senescence. (anti-agingfirewalls.com)

Volumetric analysis of formation of the complex of G-quadruplex DNA with hemin using high pressure. (bvsalud.org)
DNA guanine-quadruplexes ( G-quadruplexes ) complexed with the Fe-containing porphyrin , hemin ( iron (III)-protoporphyrin IX), can catalyze oxidation reactions. (bvsalud.org)
To improve DNAzyme properties, we sought to elucidate the interaction mechanism between G- quadruplex DNA and hemin . (bvsalud.org)
The G- quadruplex DNA alone and the G- quadruplex DNA - hemin complex were destabilized with increasing pressure in Na+ buffer . (bvsalud.org)
The pressure required to destabilize the h-telo- hemin complex was less in K+-containing buffer than in buffer with Na+, which indicates that there was a smaller volumetric change upon h-telo formation in K+ buffer than in Na+ buffer . (bvsalud.org)
CEA aptamer loaded onto Pt@CuMOFs was bound with hemin to form hemin@G-quadruplex (hGq) with mimicking peroxidase activity. (unboundmedicine.com)

We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). (soton.ac.uk)

Template-mediated stabilization of a DNA G-quadruplex formed in the HIV-1 promoter and comparative binding studies. (univ-grenoble-alpes.fr)

However, the technique used required either killing the cells or using high concentrations of chemical probes to visualize G4 formation, so their actual presence within living cells under normal conditions has not been tracked, until now. (barfblog.com)
We next employed chemistry-based oligonucleotide probes to mimic the naturally formed telomeric DNA-RNA G-quadruplexes in living cells, suggesting that the process of DNA-RNA G-quadruplex formation with oligonucleotide models of telomeric DNA and RNA could occur in cells. (elsevierpure.com)

G-quadruplexes have been shown to be involved in a range of processes including telomere stability and RNA transcription, splicing, translation and transport. (nature.com)

Depending on how the individual runs of guanine bases are arranged in a bimolecular or intramolecular quadruplex, a quadruplex can adopt one of a number of topologies with varying loop configurations. (wikipedia.org)

ATRX promotes heterochromatin formation to protect cells from G-quadruplex DNA-mediated stress. (nih.gov)

It has emerged from recent studies that G-quadruplexes occur widely throughout the human genome and have significant biological roles. (herts.ac.uk)
In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)
However, the presence of the ligand Phen-Se did not appear to have a significant effect either on G-quadruplex binding or on biological activity. (telomerescience.com)

Here, we examine the bimolecular diagonal-looped foldback quadruplexes [d(G3T4G3)]2 (Q3) and [d(G4T4G4)]2 (Q4) by 2H, 17O, and 23Na magnetic relaxation dispersion (MRD). (lu.se)
can be used to probe the internal ions of quadruplexes in We have chosen to study the bimolecular foldback aqueous solution. (lu.se)

If all strands of DNA proceed in the same direction, the quadruplex is termed parallel. (wikipedia.org)
Schematic representation of the parallel stranded GGGGCC RNA G-quadruplex. (nature.com)

The placement and bonding to form G-quadruplexes is not random and serve very unusual functional purposes. (wikipedia.org)
The GGGGCC expansion of C9orf72 forms runs of adjacent G-repeats and raises the possibility these could form G-quadruplexes. (nature.com)
The new paradigm or exception to this rule is that multiple guanine nucleotides also spontaneously form a "tetraplex" with a positively charged cation, such as potassium, forming what is referred to as a "Guanine Qaudruplex", or "G-quadruplex" for short. (anti-agingfirewalls.com)
The experimental data produced by this technique provides unprecedented details on where and at what intensity do G-quadruplexes form in the human genome. (bgu.ac.il)

An application note from Thermo Fisher Scientific describes the use of the company's NanoDrop 3300 instrument for studying DNA methylation events and polyamine induced DNA G-quadruplex formations by microvolume fluorometry. (spectroscopyonline.com)

Further analysis by fluorescence emission spectroscopy confirmed G-quadruplex formation in the human and bovine FXYD1-oligonucleotides that was triggered by the presence of K+ ions. (herts.ac.uk)
Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. (ntu.edu.sg)

The MRD data indicate that both quadruplexes contain Na+ ions between the T4 loops and the terminal G-quartets and that these ions have one water ligand. (lu.se)
In both quadruplexes, the stem ions have residence times of 0.6-1.0 ms at 27 °C. The equilibrium constant for Na+ f K+ exchange is 4 for both loop and stem sites in Q3, in agreement with previous 1H NMR findings. (lu.se)
7 ) and Tl+ ( 8 ) ions have been quadruplexes formed by the oligodeoxyribonucleotides used as a spin-1/2 surrogates for the similarly sized K+ ion, d(G3T4G3) (abbreviated Q3) and d(G4T4G4) (abbreviated Q4). (lu.se)

However, as currently used in molecular biology, the term G4 can mean G-quadruplexes of any molecularity. (wikipedia.org)
There have been a flurry of publications on the method this year, and it is so new that it didn't make it into a February Molecular BioSystems review of isothermal methods or a December 2013 Methods review of quadruplex-based methods for nucleic acid detection. (genomeweb.com)
Štefl R., Cheatham T. E, Špačková, N., Fadrná E., Berger I., Koča J., Šponer J.: Formation pathways of a guanine-quadruplex DNA revealed by molecular dynamics and thermodynamic analysis of the substates. (learned.cz)

G-quadruplex formation in human and bovine FXYD1-derived oligonucleotides was detected experimentally by non-denaturing poly acrylamide gel electrophoresis that showed an increased mobility rate of G-quadruplexes in contrast to controls. (herts.ac.uk)

In an important model of premature aging, Werner's Syndrome, the genetic problem seems to be lack of a helicase protein that can unwind G-quadruplexes. (anti-agingfirewalls.com)
Carrier Protein Mediated Formation of the Dihydropyridazinone Ring in Actinopyridazinone Biosynthesis. (go.jp)
5,5-dmbpy = 5,5′-dimethyl-2,2′-bipyridine) exhibited high luminescence for G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a G-quadruplex-based assay for protein tyrosine kinase-7 (PTK7) in aqueous solution. (edu.hk)

To our knowledge, this is the first G-quadruplex-based assay for PTK7. (edu.hk)
Furthermore, the cell migration assay and the tube formation assay also demonstrated that the complex Ru-Se significantly inhibited human umbilical vascular endothelial cell (HUVEC) proliferation, migration, and tube formation. (telomerescience.com)

Single-molecule visualisation of DNA G-quadruplex formation in live cells. (ecbs2021.eu)

A study of Sapienza University in collaboration with the Italian Institute of Technology sheds new light on the lesser-known part of our genetic makeup, highlighting the action mechanism underlying a non-coding RNA molecule in muscle tissue formation. (uniroma1.it)

Further in-vivo works should address whether alternative splicing of FXYD1 to FXYD1-009 is associated with G-quadruplex formation. (herts.ac.uk)

This natural 8-ring 24-membered macroheterocycle (Figure 2 ) displays high affinity for quadruplex and most importantly has no affinity for duplex DNA ( vide infra ). (hindawi.com)

The formation of four-stranded DNA has been tracked in living human cells, allowing scientists to see how it works, and its possible role in cancer. (barfblog.com)

Furthermore, we investigated the possible roles of this DNA-RNA G-quadruplex. (elsevierpure.com)

C9orf72 and triplet repeat disorder RNAs: G-quadruplex formation, binding to PRC2 and implications for disease mechanisms. (colorado.edu)

93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. (ntu.edu.sg)

Anatomy1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes9

Information Science2

Health Care1

Structures35

Discovering G-quadruplex structure1

Telomeric G-quadruplex2

Sequences11

Intramolecular quadruplexes2

Ligands6

Stable3

Interestingly2

Vitro3

MRNA1

Telomerase5

Structure17

Scaffolds2

Small Molecules3

Genomic instability1

Oncogenes1

Sequence3

Abstract1

Complexes2

Recognition1

Antiparallel1

Polymorphism1

Promoters2

Interactions1

Tetramolecular2

Single-stranded1

Hemin6

Triplexes1

Stabilization1

Probes2

Telomere1

Topologies1

ATRX1

Biological3

Bimolecular2

Parallel2

Form4

Methylation1

Fluorescence2

Ions3

Molecular3

Oligonucleotides1

Protein3

Assay2

Visualisation1

Mechanism1

Vivo1

Duplex1

Human1

Roles1

Implications1

Enzyme1