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  • pathogenesis
  • In Vibrio cholerae, multiple QS circuits control pathogenesis and biofilm formation. (nih.gov)
  • The laboratory of Lauren Bakaletz, PhD, director, Center for Microbial Pathogenesis and vice president of basic sciences, The Research Institute at Nationwide Children's, studied the biofilm construction capabilities of nontypeable Haemophilus influenzae (NTHI), a bacterium responsible for sinusitis, pneumonia, exacerbations of cystic fibrosis and COPD, bronchitis and ear infections. (phys.org)
  • adherence
  • Time course analysis using a formazan salt reduction assay to monitor metabolic activities of cells within the biofilm, together with microscopy studies, revealed that biofilm formation by C. dubliniensis occurred after initial focal adherence, followed by growth, proliferation, and maturation over 24 to 48 h. (asm.org)
  • Both attachment to plastic and mat formation require Flo11p, a member of a large family of fungal cell surface glycoproteins involved in adherence. (sciencemag.org)
  • biomass
  • Exposure of cells to the SPIONs at concentrations up to 200 μg/ml resulted in an increase in biofilm biomass by 16 h under static conditions and a corresponding increase in cell density in the bulk liquid. (scirp.org)
  • sessile
  • Initial attachment of cells is followed by proliferation and biofilm formation, with sessile cells in biofilms displaying properties that are dramatically different from their planktonic (free-living) counterparts. (asm.org)
  • aggregates
  • EPS make up the intercellular space of microbial aggregates and form the structure and architecture of the biofilm matrix. (mdpi.com)
  • epidermidis
  • Here we report on the function of novel 18 kDa Small basic protein (Sbp) that was isolated from S. epidermidis biofilm matrix preparations by an affinity chromatographic approach. (nih.gov)
  • In this report, a biofilm model of S. epidermidis was generated and the minimal inhibitory concentration (MIC) and sub-MIC (SMIC) on bacterial cultures were assessed for the following agents: total alkaloids of Sophora alopecuroides (TASA), ciprofloxacin (CIP), and erythromycin (ERY). (hindawi.com)
  • These results thus suggest that TASA has an inhibitory effect on biofilm formation of clinic S. epidermidis , which may be a potential agent warranted for further study on the treatment prevention of infection related to S. epidermidis in veterinary clinic. (hindawi.com)
  • morphology
  • Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). (hindawi.com)
  • crude
  • Affinity purification of Aap domain-B interaction partners from crude biofilm matrix preparations. (nih.gov)
  • Vibrio
  • The quorum sensing (QS) pathway of Vibrio cholerae activates the expression of VpsR, VpsT and AphA (the main activators of biofilm formation) at low cell density and HapR (the main repressor) at high cell density. (genome.jp)
  • A Vibrio cholerae autoinducer-receptor pair that controls biofilm formation. (nih.gov)
  • characterize
  • Scanning electron microscopy and confocal scanning laser microscopy were used to further characterize C. dubliniensis biofilms. (asm.org)
  • CLSM
  • A morphological analysis using CLSM further confirmed the destruction of biofilm by these agents. (hindawi.com)
  • confocal
  • At various times, the medium was shifted to medium supplemented with DNase I, and the fate of the biofilms was followed by confocal laser scanning microscopy ( Fig. 1 ). (sciencemag.org)
  • After 12, 36, 60, and 84 hours, the medium was shifted to minimal medium supplemented with DNase I, and the fate of the biofilms was followed by recording confocal laser scanning micrographs ( 8 ) at various times after the shift. (sciencemag.org)
  • Surface
  • The ability of MS to adhere and grow on a glass surface, reflecting biofilm formation, was studied in the presence of the lactobacilli (LB). The effect of LB culture supernatants on the viability of the MS was studied as well. (springer.com)
  • The second phase of the study investigated surface characteristics of implants that could influence the formation of a biofilm. (bl.uk)
  • environments
  • Here, we review selected case studies to exemplify this potential, discuss limitations, and suggest that this approach opens new vistas into biofilm research over traditional setups, allowing us to expand our understanding of the formation and consequences of biofilms in a broad range of environments and applications. (asm.org)
  • These idealized conditions are often not realized in practice, and the explicit consideration of additional elements of natural biofilm environments has recently begun to reveal important new features of this microbial lifestyle ( 4 ). (asm.org)
  • grown
  • Most manifestations of candidiasis are associated with biofilm formation, with cells in biofilms displaying properties dramatically different from free-living cells grown under normal laboratory conditions. (asm.org)
  • strain
  • Conversely, we observed a natural rough variant of M. colombiense (57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. (hindawi.com)
  • cells
  • Fe(II) levels in the supernatants of biofilms formed in the presence of FeNPs exceeded 100 μM compared with 20 μM in control media without cells. (scirp.org)
  • Mature C. dubliniensis biofilms consisted of a dense network of yeasts cells and hyphal elements embedded within exopolymeric material. (asm.org)
  • The latter has been presumed to be derived from lysed cells and has not been thought to represent an important component of biofilm structure. (sciencemag.org)
  • However, the matrix composition, spatial organization, and relevance of specific molecular interactions for integration of bacterial cells into the multilayered biofilm community are not fully understood. (nih.gov)
  • latter
  • The source of this DNA is unclear, but it is presumably derived from membrane vesicles rather than cell lysis as we saw no evidence of the latter during biofilm formation. (sciencemag.org)
  • matrix
  • The matrix, which holds bacterial biofilms together, is a complex mixture of macromolecules including exopolysaccharides, proteins, and DNA ( 4 ). (sciencemag.org)
  • This suggests that the matrix in mature biofilms may be strengthened by other substances or that mature biofilms may produce sufficient proteolytic exoenzymes to locally inactivate the DNase I ( 7 ). (sciencemag.org)
  • Sbp accumulates within the biofilm matrix, being preferentially deposited at the biofilm-substratum interface. (nih.gov)
  • fundamental
  • These biofilms then play a fundamental role in determining the degradation of the particles and, ultimately, the fate of carbon in the particles, with a direct impact on the flux of carbon from the upper to the deep ocean ( 9 ). (asm.org)
  • A graphic view of some environmental factors that can be fundamental for biofilm formation. (asm.org)
  • Part of Scott's research is on the fundamental processes of biofilm formation, one of the major frontiers in wound healing science today. (woundsource.com)
  • spots
  • Upper part, particles are prime microbial resource hot spots and biofilm substrates in the ocean. (asm.org)
  • C) Detachment of IA5 biofilm colonies by dispersin B. A well containing a small number of biofilm colonies (dark spots) was aspirated and filled with 100 μL of dispersin B (20 μg/mL in PBS) and photographed at the indicated times. (nih.gov)
  • Burkholderia
  • Here we establish that, in addition to mediating interbacterial competition, the Burkholderia thailandensis CDI system exoprotein BcpA is required for biofilm development. (nih.gov)
  • represses
  • The VarS/VarA signaling system responds to an unknown environmental cue and represses biofilm production by process which involves the regulatory sRNAs CsrB, CsrC and CsrD and the RNA-binding protein CsrA, interfering with LuxO of the QS pathway. (genome.jp)