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  • embryos
  • Procedures for in vitro fertilization (IVF) of rat oocytes were developed and validated by transferring the resulting embryos to recipient females. (uwo.ca)
  • Although embryos resulting from IVF were less successful in establishing pregnancy than appropriate in vivo fertilized controls, optimization of culture and transfer techniques enabled minimization of embyronic losses and permitted valid use of these techniques. (uwo.ca)
  • In vitro production of embryos in swine. (publish.csiro.au)
  • It also serves as a fundamental technique in the preparation of recipients and donors for embryo transfer procedures, as well as in the recovery of a large number of embryos used later for both biotechnological purposes and in basic studies. (biomedcentral.com)
  • MHR 2014) ePAB orthologues expressed in oocytes and early embryos. (yale.edu)
  • While Epab -/- males and Epab +/- of both sexes were fertile, Epab -/- female mice were infertile, and could not generate embryos or mature oocytes in vivo or in vitro . (yale.edu)
  • In the United States, a mean number of 2.45 embryos were transferred in IVF cycles using fresh non-donor oocytes in 2006, resulting in a 34.3% live birth rate per transfer, of which 32% were multiple-infant live births. (yale.edu)
  • Similarly, a mean number of 2.3 embryos were transferred in IVF cycles using fresh donor oocytes, achieving a 52.3% live birth rate per transfer, 40.8% of which were multiple-infant live births. (yale.edu)
  • Our cytoplasmic injection protocol improved generation of transgenic embryos and resulted in the first transgenic buffalo calves produced by this method. (biomedcentral.com)
  • We are examining whether pregnancy rates differ based on day of embryo transfer in patients who replace all available embryos after an In vitro Fertilization (IVF) cycle. (stanford.edu)
  • Research with rodent species continues to provide the bulk of basic information on early development, in large part because of the ready availability of oocytes and embryos, but it is difficult to know to what extent information can be extrapolated to primate species, including humans. (springer.com)
  • However, the amount and quality of basic scientific data on early development that can be obtained from this source are restricted, in part by ethical constraints (Austin, 1990) and in part by conflicting priorities between research and clinical needs for the supply of oocytes and embryos. (springer.com)
  • These are then punctured with a specialised needle under ultrasound guidance to retrieve eggs, which are then fertilised in a petri dish ('in vitro') to create several embryos. (medfem.co.za)
  • antral follicles
  • The follicles are named preantral or antral follicles, according to the absence or presence of a cavity, respectively. (biomedcentral.com)
  • In addition, late antral follicles in the ovaries of Epab -/- mice exhibited impaired cumulus expansion, and a 8-fold decrease in ovulation, associated with a significant down-regulation of mRNAs encoding EGF-like growth factors AREG, EREG, and BTC, and their downstream regulators, PTGS2, HAS2, and TNFAIP6. (yale.edu)
  • Enzymatically isolated pre-antral follicles from the ovaries of 10-12 day SJL pups were divided amongst control (CT) and HA treatments. (biomedcentral.com)
  • ovaries
  • Storage of cat ovaries for up to 24 h caused minimal alteration of preantral follicles and oocytes. (biomedcentral.com)
  • Other studies reported unaffected in vitro maturation (IVM) rates when domestic cat ovaries were stored at 4 °C up to 72 h. (biomedcentral.com)
  • 19 ] isolated the so-called "rare mitotically active germ cells" from adult mouse and human ovaries and propagated them in vitro , which after all generated oocytes. (biomedcentral.com)
  • In Low Dose IVF the ovaries of a woman are minimally stimulated, using oral ovulation induction agents and/or low doses of gonadotropins (follicle stimulating hormone and luteinizing hormone), in order to induce the growth of a small number of eggs. (medfem.co.za)
  • vitrification
  • Previous studies showed that oocytes submitted for vitrification or slow freezing induced subcellular alterations or disturbances in the intrinsic cellular homeostasis as compared to untreated controls. (biomedcentral.com)
  • pregnancy
  • The results indicate no difference in pregnancy or birth rates when oocytes collected from large or small folluicles were used in goat SCNT. (usda.gov)
  • Many of these PCOS women require techniques of assisted reproduction to achieve a pregnancy. (hindawi.com)
  • However, more than 60% of in vitro fertilization (IVF) cycles do not result in a pregnancy and poor oocyte quality is the main cause of fertilization failure in assisted reproductive techniques [ 14 ]. (hindawi.com)
  • Although hMGs increased the number of oocytes and the chance of pregnancy, they brought about Ovarian Hyperstimulation syndrome (OHSS), which could even be fatal for an otherwise healthy young woman (16) . (tjoddergisi.org)
  • The time of ovulation was determined and a pregnancy diagnosis was performed 14 days after ovulation of the injected follicle. (biomedcentral.com)
  • Endometriosis appears to affect every part of a woman's reproductive system including ovarian function, oocyte quality, embryo development and implantation, uterine function and the endocrine system choreographing the reproductive process and results in infertility or spontaneous pregnancy loss. (biomedsearch.com)
  • One of the predominant factors affecting proper embryo development and successful pregnancy is oocyte quality. (biomedcentral.com)
  • primordial follicles
  • Strikingly, BOLL is downregulated, and DAZL re-expressed, as primordial follicles form, revealing BOLL expression to be restricted to a narrow window during fetal oogenesis. (jove.com)
  • stimulation
  • As the number of retrieved oocytes was smaller in the myoinositol group, the risk of hyper stimulation syndrome can be reduced in these patients. (hindawi.com)
  • Follicle-stimulating hormone (FSH) stimulation of in vitro maturing oocytes delayed nuclear maturation but did not affect the proportion of oocytes capable of undergoing fertilization. (uwo.ca)
  • Exogenous gonadotropin stimulation for short courses seems to improve the ultrastructure of the oocytes expected to mature in in vitro conditions. (tjoddergisi.org)
  • Epab -/- oocytes failed to achieve translational activation of maternally-stored mRNAs upon stimulation of oocyte maturation, including cyclin B1 and Dazl mRNAs. (yale.edu)
  • This is a randomized open-label study to compare between in-vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes of the two regimen of Cetrotide® (Cetrorelix acetate) which are 0.25 milligram (mg) used from Day 1 or Day 7 of the menstrual cycle (Day 0 or Day 6 of stimulation) in polycystic ovarian (PCO) women in assisted reproductive technology (ART). (clinicaltrials.gov)
  • Low Dose IVF, a minimal stimulation IVF, is a relatively new and advanced reproductive technology (ART) that offers a gentle alternative of ovarian stimulation to traditional in vitro fertilisation (IVF). (medfem.co.za)
  • fertility
  • However, studies have shown that IVM can be used in almost all areas where in vitro fertilization (IVF) is used and it has a strong place in fertility protection and Ovarian Hyperstimulation syndrome management. (tjoddergisi.org)
  • The purpose of this Funding Opportunity Announcement (FOA) is to encourage applications from the scientific community to support outstanding research in the area of oocyte mitochondrial function in relation to fertility, aging, and mitochondrial disease transmission to offspring. (nih.gov)
  • In vitro follicle techniques provide a tool to model follicle development in order to investigate basic biology, and are further being developed as a technique to preserve fertility in the clinic 1-4 . (jove.com)
  • We offer a range of comprehensive fertility treatments and techniques including: IVF, ICSI-ET, FET, PGD, blastocyst biopsy and IVF-surrogacy. (diffhospital.com)
  • embryonic
  • Oocytes matured in the presence of their cumulus cells and serum were as capable of IVF, embryonic and fetal development as ovulated oocytes. (uwo.ca)
  • The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. (scielo.org.co)
  • Embryonic poly(A) binding protein (ePAB), identified in Xenopus oocytes (Voeltz et al. (yale.edu)
  • sperm
  • Oocytes matured in the absence of cumulus cells, serum or follicular fluid had an increased resistance to sperm penetration. (uwo.ca)
  • IVF refers to a technique of assisted reproduction where the egg and sperm are fertilised outside of the body to form an embryo. (medfem.co.za)
  • ICSI involves use of specialized micromanipulation tools and techniques by our embryologists to select individual sperm with an ICSI needle and inject it directly into the inner part of an egg. (diffhospital.com)
  • small follicles
  • The dynamic mechanical environment mimics the natural ovarian environment in which small follicles reside in a rigid cortex and move to a more permissive medulla as they increase in size 11 . (jove.com)
  • morphology
  • In Experiment 1, the effects of the storage period at 4 °C on the morphology, cytoskeleton (α/β tubulin) and DNA integrity (phosphorylation of histone H2AX) of preantral follicles were investigated. (biomedcentral.com)
  • Interpretation of equine in-vitro produced embryo morphology (Abstract). (tamu.edu)
  • Outcome parameters monitored were follicle morphology, survival after IVC, antrum formation, GVBD and MII formation. (biomedcentral.com)
  • western blot
  • Isolated follicles can be used for a variety of experimental techniques, including in vitro development assays, live imaging, mRNA expression analysis and western blot analysis of proteins. (jove.com)
  • Additionally, protein level, stability, and/or posttranslational modification state during a particular stage of follicle development can be examined through western blot analyses. (jove.com)
  • generation of transgenic
  • Because generation of transgenic and gene-targeted mouse mutants is costly and can take long periods of time, manipulation of female gametes in vitro is a more economical and time-saving strategy. (jove.com)
  • morphological
  • Additionally, because these follicles develop in culture, they are ideally suited for live imaging studies, which often reveal new mechanisms that mediate morphological events. (jove.com)
  • PURPOSE: The objective of our study is to assess the relationship of embryo ploidy status in relation to embryo sex, morphological characteristics, and transfer parameters.METHODS: This is a retrospective cohort study at an academic medical center of patients who underwent in vitro fertilization with preimplantation genetic screening (PGS) from 2010 to 2015. (stanford.edu)