• Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • The number of ovarian follicles and corpora lutea also were not affected by treatment. (ejast.org)
  • Transvaginal ovum retrieval (OVR) is the process whereby a small needle is inserted through the back of the vagina and guided via ultrasound into the ovarian follicles to collect the fluid that contains the eggs. (wikipedia.org)
  • The process involves monitoring and stimulating a woman's ovulatory process, removing an ovum or ova (egg or eggs) from her ovaries and letting sperm fertilise them in a culture medium in a laboratory. (wikipedia.org)
  • Ovaries from abattoirs are grouped into four categories: ovaries without corpus luteum and dominant follicles (-CL and -FD), without corpus luteum and with dominant follicles (-CL and + FD), with corpus luteum and without dominant follicle (+ CL and -FD), and with corpus luteum and dominant follicles (+ CL and + FD). (ifory.id)
  • Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
  • AMH stands for Anti-Müllerian Hormone, which is produced by follicles in the ovaries which contain eggs. (worldmedic-ivf.com)
  • In affected ovaries, oxygenation of follicular fluid is low, ultrastructural disturbances especially of mitochondria, occur in granulosa cells and oocytes, and considerable disturbances of meiosis occur. (biomedcentral.com)
  • A health care provider closely monitors the development of the eggs using transvaginal ultrasound and blood tests to assess follicle growth and estrogen production by the ovaries. (wikipedia.org)
  • Oocytes were collected from aspirated porcine tertiary follicles. (biomedcentral.com)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
  • Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
  • Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)
  • The problems mostly commonly occur when the ovarian pool of follicles is depleted to a critical level with age but are also found in low follicular reserve of other etiologies. (biomedcentral.com)
  • As well as inducing a plethora of deleterious changes in follicular cytoplasmic structure and function, the omega 9 palmitate/oleate ratio is increased by lowered activity of PPARα. (biomedcentral.com)
  • Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
  • Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
  • After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)
  • Oocytes 5-6 after fertilization were washed 3 times using CR1aa media then cultured in 5% CO2 incubator, temperature 38.5 °C for 48 hours. (ifory.id)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
  • Components and OPTIONS FOR in vivo test: In vitro maturation and fertilization maturation and fertilization had been carried out as described [21] previously. (bioskinrevive.com)
  • The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)
  • In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
  • ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
  • This subject involves procedures such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), cryopreservation of gametes or embryos, and/or the use of fertility medication . (wikipedia.org)
  • With ART, the process of sexual intercourse is bypassed and fertilization of the oocytes occurs in the laboratory environment (i.e., in vitro fertilization ). (wikipedia.org)
  • In vitro fertilization is the technique of letting fertilization of the male and female gametes (sperm and egg) occur outside the female body. (wikipedia.org)
  • The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
  • After that, we examined the result of cultured conditioned mass media (CM) of IVF embryos on splenic immune system cells and Madin-Darby bovine kidney (MDBK) cells with stably presented ISG15 promoter-reporter constructs. (bioskinrevive.com)
  • For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
  • Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
  • In some species (e.g., bovine), evidence indicates that decreases in IGFBP-4 and -5 levels in estrogen-dominant preovulatory follicles are likely due, in part, to increased protease activity, whereas lower levels of IGFBP-2 are not due to increased proteolysis. (bioone.org)
  • Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)
  • Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)
  • It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
  • Studies that compared postnatal outcomes between in vitro-conceived (IVF, ICSI or IVM) and in vivo-conceived mammalian animal models were included. (bvsalud.org)
  • When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)
  • Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
  • Today, the scientific term "in vitro" is used to refer to any biological procedure that is performed outside the organism in which it would normally have occurred, to distinguish it from an in vivo procedure (such as in vivo fertilisation), where the tissue remains inside the living organism in which it is normally found. (wikipedia.org)
  • This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
  • In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
  • In vivo conception included mating, artificial insemination, or either of these followed by embryo transfer to a recipient animal with or without in vitro culture. (bvsalud.org)
  • WIDER IMPLICATIONS: Conception via in vitro or in vivo means appears to have an influence on measurable outcomes of offspring physiology, manifesting differently across the species studied. (bvsalud.org)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
  • Since recent clinical studies have indicated that dehydroepiandrosterone (DHEA) supplementation may reverse the problem of oocyte quality, this review of the literature was undertaken in an attempt to find an explanation of why this is effective? (biomedcentral.com)
  • These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
  • For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
  • In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)
  • To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
  • Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)
  • thereby, oocytes and sperm are highly vulnerable to reactive oxygen species (ROS) attacks, affecting their quality and viability (6). (vetmedmosul.com)
  • The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
  • As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)
  • To characterize the effect of artificially increasing MIR21 on oocyte competence without inhibiting GVBD, a MIR21 mimic, scrambled microRNA negative control, or nuclease free water was micro-injected into denuded oocytes at 21 h of IVM. (biomedcentral.com)
  • Therefore, a potential conflict was observed between low-fat diets, which stimulate follicle growth and ovulation, and high-fat diets (insulin-depressing), which improve the development of the embryo and pregnancy outcome [ 15 ]. (ejast.org)
  • When follicles have reached an adequate size and the eggs are mature enough, an injection of the hormone hCG initiates the ovulation process. (wikipedia.org)
  • In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
  • Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)
  • The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
  • In Vitro Fertilisation (IVF) is a procedure in which fertilisation occurs outside the body. (worldmedic-ivf.com)
  • The oocytes compete with one another to become the dominant follicle and eventually ovulate to be released as an egg capable of being fertilized. (medscape.com)
  • A role for the complex in mammalian synapse formation and neurite outgrowth has been suggested by the early presence of Sec6/Sec8 immunoreactivity at sites of synaptogenesis in culture (Hazuka, 1999) and the impairment of neurite outgrowth with dominant-negative forms of sec10 and sec8 in PC12 cells (Vega, 2001). (sdbonline.org)
  • Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
  • Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)
  • In vitro effects of PCBs and OH-PCBs on the basal and dexamethasone-modified thyroid hormone metabolism in chicken liver. (krakow.pl)
  • In Vitro" is a Latin phrase meaning "in glass," which relates to the laboratory dishes used for fertilisation instead of the uterus. (worldmedic-ivf.com)
  • and human spermatogenic stem cell culture to treat azoospermia, and to preserve fertility in pre-pubertal boys undergoing cancer treatment. (infertile.com)
  • In females with normal fertility, pre-antral ovarian theca cells respond to stimulation by inhibin B to provide androgen-based support for the developing follicle. (biomedcentral.com)
  • Moreover, the in vitro experiment demonstrated high glucose amounts' deleterious effect on embryo development [ 3 ]. (ejast.org)
  • The Latin term in vitro, meaning "in glass", is used because early biological experiments involving cultivation of tissues outside the living organism were carried out in glass containers, such as beakers, test tubes, or Petri dishes. (wikipedia.org)
  • Despite considerable heterogeneity, these studies suggest that the use of IVF or maturation results in offspring with higher birthweights and a longer length of gestation, with most of this evidence coming from studies in cattle. (bvsalud.org)
  • The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • The adaptor protein Grb2 is able to enhance the activity of the cytoplasmic tyrosine kinase Btk through a novel mechanism, revealing a new role for Grb2 in B-cell signaling. (elifesciences.org)
  • Thus, common ART interventions may have long-term consequences for those conceived through these techniques, regardless of the pathology underpinning diagnosed infertility. (bvsalud.org)
  • After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)
  • The selected oocytes are then matured in an incubator of 5% CO2, a temperature of 38.5 °C for 24 hours. (ifory.id)
  • However, IVF is usually performed in Petri dishes, which are both wider and shallower and often used to cultivate cultures. (wikipedia.org)
  • Each petri dish contained three drops of maturation medium (300 µl/drops) according to the groups. (sciencegate.app)
  • This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)
  • In 2018, it was estimated that eight million children had been born worldwide using IVF and other assisted reproduction techniques. (wikipedia.org)
  • Oocyte arrest at the diplotene stage is maintained in part through the activity of phosphodiesterase enzymes that catalyze the hydrolysis of cyclic adenosine monophosphate (cAMP) into AMP [ 1 , 2 ]. (biomedcentral.com)
  • The TPP1/POT1 heterodimer also promotes telomerase processivity, as demonstrated by an in vitro direct telomerase activity assay [ 4 , 20 ]. (prolekarniky.cz)
  • After culturing, the adhesion activity of the isolated osteoclasts with the substrates was estimated with a light microscope, and the surfaces of the bone slices were observed with a scanning electron microscope. (go.jp)
  • The results showed that the adhesion activity of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts is higher at the bone resorptive phase than at the bone formative phase, and this tendency in isolated osteoclasts was observed more frequently on the bone slices than on the glass culture dishes. (go.jp)
  • These techniques may also impair glucose and lipid metabolism in male mice. (bvsalud.org)
  • The embryologist find out the healthy, normal eggs and puts them to small culture dishes and returns it to the incubators. (drjatinshah.com)
  • The clinci has 10 incubators and it is very important that the transfer of eggs from culture dishes to incubators is done in the shortest possible time. (drjatinshah.com)
  • This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
  • After that, oocytes are collected by slicing and only oocytes with a cytoplasmic are homongent and have compact cumulus cells used in the study. (ifory.id)
  • Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
  • First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
  • The cells were cultured on glass culture dishes and bone slices. (go.jp)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
  • Our results indicate MIR21 is active and important during meiotic maturation of the oocyte. (biomedcentral.com)
  • In vitro maturation allows female goat (does) contributes toward reproduction despite the fact that the animal has been slaughtered. (sciencegate.app)
  • Reduced quality and accelerated follicle loss with female reproductive aging - does decline in theca dehydroepiandrosterone (DHEA) underlie the problem? (biomedcentral.com)
  • The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
  • We showed previously that cytoplasmic release of mtDNA activates the cGAS STING TBK1 pathway resulting in interferon-stimulated gene (ISG) expression that promotes antiviral immunity4. (regenerativemedicine.net)
  • However, PDMS has not previously been amenable to applications which require complex geometries in three dimensions for cell culture device fabrication in the absence of additional components. (bvsalud.org)
  • Relative abundance of mature MIR21 was quantified at 0, 8, 16, 24, 32, and 42 h of in vitro (IVM) with or without treatment with 3-isobutyl-1-methylxanthine (IBMX). (biomedcentral.com)
  • Oocytes were considered mature when they reached metaphase II. (sciencegate.app)