Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). (nih.gov)
Snapshots of actin and tubulin folding inside the TRiC chaperonin. (ox.ac.uk)
A second mechanism has been described for the eukaryotic chaperonin CCT, which interacts mainly with the cytoskeletal proteins actin and tubulin. (icr.ac.uk)
The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo. (google.ro)
C,D) Cryoelectron microscopy (cryo-EM) reconstructions from the chaperonin CCT (blue) in complicated with (C) actin and (D) tubulin (both in crimson). (phytid.org)
Intermediates4
Cofactor D is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. (wikipedia.org)
Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. (wikipedia.org)
The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. (thermofisher.cn)
Folding intermediates, and nonnative protein species generally, are aggregation-prone usually, both and in the congested cellular environment. (phytid.org)
Beta-tubulin1
interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. (wikipedia.org)
Alpha-tubulin1
Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)
TRiC2
The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). (nih.gov)
The TRiC complex plays a role in the folding of actin and tubulin (Probable). (nih.gov)
Tubulin-specific chaperone D is a protein that in humans is encoded by the TBCD gene. (wikipedia.org)
These findings suggest coevolution of CCT with actin and tubulin in order to counteract the folding problems associated with the generation in these two cytoskeletal protein families of new domains involved in their polymerization. (icr.ac.uk)
The fold recognition server Phyre2 ( http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index ) was used to identify the best structural template and domain of ATP7B protein, and molecular graphics were analyzed by Pymol software. (beingmedicos.com)
The microtubule protein tubulin regulates adenylyl cyclase and phospholipase Cβ 1 (PLCβ 1 ) signaling via transactivation of the G-protein subunits Gαs, Gαi1, and Gαq. (jneurosci.org)
Because most tubulin is not membrane associated, this study investigates whether tubulin translocates to the membrane in response to an agonist so that it might regulate G-protein signaling. (jneurosci.org)
These observations presented a spatial and temporal resolution of the sequence of events underlying receptor-evoked involvement of tubulin in G-protein-mediated signaling. (jneurosci.org)
The interaction of tubulin with these polypeptides involves a GTP transfer from the exchangeable GTP-binding site (E site) of tubulin to Gα, which activates the G-protein (transactivation) ( Roychowdhury and Rasenick, 1994 ). (jneurosci.org)
G) Cryo-EM reconstruction of eukaryotic prefoldin (silver) in complicated with nonnative actin (crimson). (phytid.org)
Cytosol1
It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. (thermofisher.cn)
Polypeptides1
Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. (nih.gov)
Regulation1
Knaus and Mundlos analyse the effects of mechanical stimuli, which are so essential for bone healing, on chromatin folding and gene regulation. (charite.de)
The cadherin cytoplasmic tail binds β-catenin (Armadillo [Arm]), that links to α-catenin (α-cat), which binds F-actin. (rupress.org)
Cytoskeleton1
Dystrophin-glycoprotein complex bridges the inner cytoskeleton (F-actin) and the basal lamina. (medscape.com)
Interaction1
Here we have performed a detailed study of the docking of the actin and tubulin molecules extracted from their corresponding CCT:substrate complexes obtained from cryoelectron microscopy and image processing to localize certain regions in actin and tubulin that are involved in the interaction with CCT. (icr.ac.uk)
Complex1
Our findings provide new insights into the complex dynamics of chaperonins and their role in the functional folding cycle. (bvsalud.org)
Pathway1
Supernatant from quartz-treated macrophages rapidly activated the NF-κB signalling pathway in RLE cells and markedly induced iNOS mRNA expression up to 2000-fold compared to non-treated control cells. (biomedcentral.com)
Kinase1
When the inhibitor Rho kinase (Y27632, small GTPase), a regulator of actin polymerization, was tested, encystment of was blocked [39]. (globaltechbiz.com)
Cellular2
The 1.5-fold increase in the intensity of tubulin suggested that the ligand inhibitory effect stabilized the cellular microtubule structure. (hainmc.edu.cn)
Preliminary data with endothelial cells shows striking changes in cellular mechanics, nuclear lamina and extracellular matrix composition, as well as increased F-actin bundling and tubulinacetylation, resulting in increased cellular tension and nuclear deformation. (charite.de)
Cavity1
ATP binding liberates the substrate in the GroEL cavity where it is given a chance to fold. (icr.ac.uk)
Image1
Bottom image is a close-up view of part of the top image to clearly visualize actin rings (stripes) with 180 nm periodicity. (cytoskeleton.com)
Functional1
We consider a number of the best-characterized chaperones today, which we classify into three wide functional types: holding, unfolding and folding. (phytid.org)
Structure1
The pilus subunit includes an immunoglobulin fold that does not have a -strand, which, in the set up pilus structure, is normally supplied by the neighbouring pilus subunit. (phytid.org)
Rapid1
In SK-N-SH cells, carbachol induced a rapid and transient translocation of tubulin to the plasma membrane, microtubule reorganization, and a change in cell shape as demonstrated by confocal immunofluorescence microscopy. (jneurosci.org)
Cells2
In short, the combination of STED and SiR probes allows for unparalleled fluorescent visualization of subcellular actin and tubulin/microtubule structures and their physical characterization in living cells, (see Fig. 2 and Ref. 2). (cytoskeleton.com)
A specific and time-dependent association of tubulin with plasma membranes was observed when SK-N-SH cells were treated with carbachol. (jneurosci.org)
Activity1
The levels of adenylate cyclase activity rises 2C4-fold during dormant stage [46]. (globaltechbiz.com)
Maintenance1
However, mechanistic details are lacking, and recent studies have implicated ALMS1 in several processes including endosomal trafficking, actin organisation, maintenance of centrosome cohesion and transcription. (springer.com)
Transfer2
Tubulin, at nanomolar concentrations, transactivated Gαq by the direct transfer of a GTP analog and potentiated carbachol-activated PLCβ 1 . (jneurosci.org)
The time course of this event was concordant both with transactivation of Gαq by the direct transfer of [ 32 P]P 3 (4-azidoanilido)-P 1 -5′-GTP from tubulin as well as with the activation of PLCβ 1 . (jneurosci.org)
State1
The folding procedure is now viewed as the downward route an unstructured polypeptide assumes a funnel-like free-energy surface MGCD0103 pontent inhibitor area representing the progressively decreasing variety of conformations open to it since it gets to its indigenous state (Supper (Daggett & Fersht, 2003). (phytid.org)