Fluorochrome antibody. Medical search. Frequent questions

C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Brilliant Violet 480 (Product # 414-4031-81) (left) or 0.25 µg of CD3 Monoclonal Antibody, Brilliant Violet 480 (right). (thermofisher.com)
Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. (novusbio.com)
Antibodies were detected with serially diluted Sheep Anti-FITC-PE. (novusbio.com)
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. (novusbio.com)
FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. (novusbio.com)
There are no reviews for FITC Antibody (NB100-64617). (novusbio.com)
Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. (anobase.org)
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). (anobase.org)
Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. (anobase.org)
Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. (anobase.org)
The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface. (anobase.org)

Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)

These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. (vectorlabs.com)
Kit Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. (immunospot.com)
The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer. (immunospot.com)
Fluorochrome conjugates for (5HT2B Receptor) in the Chromocyte database. (chromocyte.com)
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. (biotium.com)
Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. (biotium.com)
For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). (biotium.com)
1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols 1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. (vectorlabs.com)

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. (rndsystems.com)
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. (vectorlabs.com)
Green Fluorescent Protein, GFP) and staining with fluorescently conjugated antibodies or fluorescent dyes. (sigmaaldrich.com)
Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
In blood cell analysis applications in both research and clinical laboratories, the cells are first treated with a number of fluorescent labels (fluorochromes). (coherent.com)

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. (cellsignal.com)

While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. (thermofisher.com)
Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). (thermofisher.com)
The B27 antibody reacts with the human interferon-γ. (biolegend.com)

The following product was used in this experiment: iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 700, eBioscience™ from Thermo Fisher Scientific, catalog # 56-5920-82, RRID AB_2848474. (thermofisher.com)
Detection of Rat IgG Control by Flow Cytometry Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG-3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB33281N , filled histogram) or Rat IgG Alexa Fluor® 700-conjugated Isotype Control Antibody (Catalog # IC013N, open histogram). (rndsystems.com)
Be the first to review Rat IgG2B Alexa Fluor® 700-conjugated Antibody and earn rewards! (rndsystems.com)
Have you used Rat IgG2B Alexa Fluor® 700-conjugated Antibody? (rndsystems.com)

The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence. (cdc.gov)
When used to adjust a flow cytometer, fluorescence intensity is converted from an arbitrary unit to the standard unit of measurement, molecules of equivalent soluble fluorochrome (MESF) units. (novusbio.com)
Setting parameters with compensation beads ensure a detector will only measure fluorescence emission from the appropriate fluorochrome, providing more confidence in the analysis of cell populations. (novusbio.com)
This is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. (salk.edu)
This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. (vectorlabs.com)

Principles of panel design dictate the optimal matching of antigen density with fluorochrome brightness, yet at least one required reagent is unavailable as a standard catalog item for approximately 20% of panels designed today. (bdbiosciences.com)
Reagent choice brings flexibility to your research, but it's important to keep in mind the BD Horizon™ Dye principles of matching antigen density with fluorochrome brightness to design optimized panels. (bdbiosciences.com)
Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. (novusbio.com)

Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)

The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)

BD OptiBuild™ On-Demand Reagents make it easy for you to gain access to the antibodies you need on an ever-growing catalog of dye combinations. (bdbiosciences.com)
BD OptiBuild™ Reagents can be used to redesign or expand upon existing panels that have various combinations of fluorochromes and antibodies. (bdbiosciences.com)
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. (vectorlabs.com)
With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel ® flow cytometry antibodies and dyes. (sigmaaldrich.com)
This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. (rndsystems.com)
740 nm, and often are reagents of choice for labeling antibodies. (vectorlabs.com)

At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity. (vectorlabs.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)

The following product was used in this experiment: CD3 Monoclonal Antibody (17A2), Brilliant Violet™ 480, eBioscience™ from Thermo Fisher Scientific, catalog # 414-0032-82, RRID AB_2925604. (thermofisher.com)

Antibodies are typically labeled through direct conjugation (either as commercially available products or conjugation kits allowing the end user to perform the reaction) or through the use of secondary antibodies. (sigmaaldrich.com)
A new proprietary flow cytometry technology allows end users to flexibly mix and match independently selected antibodies and dyes to assemble them in any combination, without the use of conjugation kits or secondary antibodies. (sigmaaldrich.com)
Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800 . (vectorlabs.com)

Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
It was prepared using affinity-purified rabbit polyclonal antibody conjugated to Cy3 fluorochrome. (atsbio.com)

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. (biolegend.com)

Other common fluorochromes like phycoerythrin & allophycocyanin are large proteins and I do not have any detailed protocols for conjugating them. (bio.net)
Epithelial immunospot (EPISPOT) is an antibody-based method for quantification of live CTCs by detection of CTCs which are capable of secreting proteins such as cathepsin D, MUC1 and CK19. (medscape.com)
These are engineered using antibody biochemistry so that they stick only to specific antigens (usually proteins) on the outside of the cells. (coherent.com)
Co-localization of 2 or more different proteins used in combination with fluorochromes of different colors can reveal the location of each protein. (cdc.gov)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
What is a Recombinant Antibody and Why is it Important? (cellsignal.com)
Recombinant antibodies offer several key advantages compared to traditional antibodies. (cellsignal.com)
As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
What is a Recombinant Antibody? (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. (biolegend.com)

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. (rndsystems.com)
Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis. (thermofisher.com)
Applications Tested: This CXNFT antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated thioglycolate-elicited peritoneal macrophages using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. (thermofisher.com)
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis . (biolegend.com)
Find answers to frequently asked questions (FAQs) about ColorWheel ® flow cytometry antibodies and dyes, straight from the experts. (sigmaaldrich.com)
Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows. (sigmaaldrich.com)
View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining. (sigmaaldrich.com)
Performing flow cytometry analysis involves using various flow cytometry antibodies and labels. (sigmaaldrich.com)
Flow cytometry antibodies are used to specifically bind to different cell markers, helping to characterize the cell type and assess any changes in protein expression as part of the experiment. (sigmaaldrich.com)
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human neutrophil granulocytes (red-filled) from C5aR2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
Separation of human monocytes (red-filled) from CD205 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD205 (HD30) PE antibody (10 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
The beads serve as a quality control for fluorochrome-conjugated monoclonal antibodies minimizing the effects of spectral spillover between multiple fluorochromes and flow cytometer channels. (novusbio.com)
Selecting fluorochromes for polychromatic panels for flow cytometry is complex and time-consuming. (biorxiv.org)

We provide species-specific secondary antibodies that are available with a variety of conjugated labels. (rndsystems.com)
We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. (rndsystems.com)
Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. (vectorlabs.com)

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. (exbio.cz)

To aid researchers in designing fluorochrome panels we implemented the algorithm in a web server, Spectracular, that also considers instrument laser configuration and allows users to define various constraints related to antibody-fluorochrome availability and marker co-expression. (biorxiv.org)

Here we present a novel complexity optimization algorithm for fast design of minimal spectral overlap fluorochrome combinations. (biorxiv.org)

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (thermofisher.com)
These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)

The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages. (exbio.cz)

A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG. (sigmaaldrich.com)
While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (vectorlabs.com)

WNV immunohistochemistry were pretreated with 0.1% wolf and a domestic dog) with severe disease associated protease (20 min at 37°C) and nonspecific antibody bind- with WNV infection. (cdc.gov)

4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent. (vectorlabs.com)
A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface. (vectorlabs.com)

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. (thermofisher.com)

The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. (bdbiosciences.com)

Poorly designed panels can result in overlap between the emission spectra of the different fluorochromes, making their signals difficult to separate. (biorxiv.org)

Cells or beads stained with individual fluorochrome-conjugated antibodies should be used for setting up compensation before running the samples. (biolegend.com)

The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. (exbio.cz)

The elimination of major xenoantigens in pig cells prevents hyperacute xenograft rejection (HXR), driven by preformed antibodies. (iupui.edu)
Transfected cells were stained with HLA-E and/or HLA-G antibody. (iupui.edu)
After the incubation period, cells are washed out and the released protein spots are detected by the incubation with a fluorochrome-conjugated antibody and counted. (medscape.com)
The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)

The isolated CTCs are then cultured in tissue culture plates pre-coated with antibodies which capture the secreted protein of interest. (medscape.com)

Fluorochrome-labeled antibodies distinguish populations and subpopulations of WBCs. (cdc.gov)

Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents. (immunospot.com)

Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. (exbio.cz)

Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
The kit also contains a NL557-conjugated Mouse Anti-Human SSEA-1 Monoclonal Antibody. (genengnews.com)
Mouse monoclonal anti-e-cadherin antibodies (BD Biosciences, San Jose, CA) in conjunction with fluorochrome-conjugated donkey anti-mouse antibodies clearly delineated sites of e-cadherin expression in lungs of rats or mice. (cdc.gov)
CA). The primary antibody was mouse anti-WNV mono- endemic for decades (4,8-11). (cdc.gov)

By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.com)

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)

Negative control for use in conjunction with R&D Systems antibodies. (rndsystems.com)

In this work, we isolate a dromedary camel V H H single-domain antibody (also known as nanobody) named D4 and a monoclonal antibody (mAb) named HM2 recognizing membrane-distal and membrane-proximal epitopes of GPC1, respectively. (nature.com)

Please make sure that your instrument is capable of detecting this fluorochrome. (thermofisher.com)

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