• I tried to do a simple staining, 1 slide with my first antibody and its fluorochrome, and the second slide with the other first antibody with its fluorochome and then check the result with the filter red and green. (utsouthwestern.edu)
  • Can I measure the concentration of a fluorochrome-conjugated antibody using an A280 reading? (rndsystems.com)
  • The A280 reading of a fluorochrome-conjugated antibody is affected by the fluorochrome as well as by the buffer used to prepare the conjugate. (rndsystems.com)
  • The instrument's value was significantly enhanced by the 1950's when Coons and Kaplan demonstrated the localization of antigens in tissues that were stained with a fluorescein (the fluorochrome) tagged antibody. (fsu.edu)
  • Perform antibody conjugation to fluorochromes for flow cytometry and metal tags for mass cytometry. (purdue.edu)
  • In this case, an antibody with an affinity to L. pneumophila bacteria, was conjugated with a fluorochrome, which is a fluorescent dye, e.g. fluorescein isothiocyanate. (cdc.gov)
  • In order to obtain optimal results from the flow cytometry analysis, especially when performing multidimensional analysis, it is vital that the appropriate combination of fluorochromes is used. (umc.edu)
  • The genome sizes of the B- and Q-types of the whitefly Bemisia tabaci (Gennnadius) were estimated using flow cytometry ( Drosophila melanogaster as the DNA reference standard and propidium iodide (PI) as the fluorochrome) and k-mer analysis. (frontiersin.org)
  • The DURAClone RE CTC Tube consists of ready-to-use tubes containing a mix of 3 antibodies conjugated to fluorochrome (Cytokeratin-FITC, CD326 (EpCAM)-APC and CD45-PC7) and a viability dye (DAPI). (beckman.com)
  • Furthermore, MA staining can be used in conjunction with commonly used fluorochromes that are detectable at the FL1 detector (FITC, GFP) of either standard or 2 laser systems. (muni.cz)
  • But it's strange because I see both color as if I had put both fluorochromes (second antibodies). (utsouthwestern.edu)
  • The concentration can be found on the lot specific Certificate of Analysis (CoA) for all Bio-Techne fluorochrome-conjugated antibodies. (rndsystems.com)
  • Circulating and BALF lymphocytes were labeled with fluorochrome-conjugated monoclonal antibodies to human lymphocyte antigens and a flow cytometer was used to count lymphocyte subtypes. (researchgate.net)
  • Each fluorochrome has distinct properties and is characterized by specific excitation and emission wavelengths. (umc.edu)
  • Multiplex immunohistochemistry (IHC) that involves more than four colors is a challenge for the microscope since the excitation and emission spectra of fluorochromes will significantly overlap. (kromnigon.com)
  • Over 30 Wavelengths - Efficient excitation of any fluorochrome. (coherent.com)
  • When samples are immunostained with more than one fluorochrome, it is crucial to have filter sets with high specificity for each fluorochrome in the sample to avoid bleed-through signals. (kromnigon.com)
  • The use of fluorochromes has made it possible to identify cells and sub-microscopic cellular components and entities with a high degree of specificity amidst non-fluorescing material. (fsu.edu)
  • To avoid spectral overlap choose, if possible, fluorochromes that have the minimal amount of emission spectral overlap. (umc.edu)
  • In general the further apart the different emission maxima of the fluorochromes are, the less spectral overlap will occur. (umc.edu)
  • In addition to the excitation/emission spectra each fluorochrome has specific properties and may be unsuitable for your particular experiment. (umc.edu)
  • In fluorescence microscopy, optical filters are used to excite (excitation filter) and collect (emission filter) light from fluorochromes. (kromnigon.com)
  • The emission filter determines which interval of the emitted light from the fluorochromes in the sample that will be passed on to the camera/eyes for detection. (kromnigon.com)
  • E.g. the emission spectra of the fluorochromes CFP and YFP partially overlap, and CFP signals also appear in the YFP fluorescence channel. (nih.gov)
  • However, it was not until the 1930's that Haitinger and others developed the technique of secondary fluorescence--employing fluorochrome stains to stain specific tissue components, bacteria, or other pathogens which do not autofluoresce. (fsu.edu)
  • These fluorochrome stains, tagged to specific objects, spurred the use of the fluorescence microscope. (fsu.edu)
  • Fluorescence measurements were converted to molecules of equivalent soluble fluorochrome for comparison. (nih.gov)
  • Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. (vectorlabs.com)
  • These results indicate that folate-modified NIR fluorochrome conjugate can be used for improved detection of FR-positive tumors. (nih.gov)
  • This means that you can custom make your own 7-plex IHC with your favorite fluorochromes without spectral unmixing corrections. (kromnigon.com)
  • Tandem conjugates are dyes that combine two fluorochromes. (umc.edu)
  • Hence, you have the freedom to combine any fluorochromes from the SpectraSplit® 7 fluorochrome chart in immunostainings, all the way up to seven colors. (kromnigon.com)
  • Use the brightest fluorochrome, typically PE or PE based energy transfer conjugates, for the protein that has the lowest expression, and vice versa the dimmest fluorochrome for the most highly expressed protein. (umc.edu)
  • Typically, each fluorochrome has a wavelength peak interval wherein most light is emitted. (kromnigon.com)
  • Fluorochrome-labeled modified albumin (MA) was applied to investigate the cellular uptake. (nih.gov)
  • Thereby the risk of bleed-through signals from the other fluorochromes rapidly increases. (kromnigon.com)
  • Hence, each filter set must be carefully designed to ignore signals from all other fluorochromes and still efficiently collect signals from its specific fluorochrome. (kromnigon.com)
  • Injection of the unmodified NIR2 fluorochrome did not result in persistent contrast increases under similar conditions. (nih.gov)
  • We hypothesized that modification of folate would retain receptor affinity in vivo, despite the bulkier NIR fluorochrome, NIR2 (em = 682 nm). (nih.gov)
  • When choosing fluorochromes, there are a number of different parameters to take into account. (umc.edu)
  • Know the specific properties of the fluorochromes you are using. (umc.edu)
  • Avoid exposing samples to bright light as many fluorochromes are light sensitive and subject to photo bleaching. (umc.edu)
  • In tandem dyes one of the fluorochromes is excited by the laser and is, in turn, able to transfer its fluorescent energy to the second molecule which then emits light of a different, longer wavelength. (umc.edu)
  • When a fluorochrome is exposed to light, a photon with the appropriate amount of energy can excite an electron in the fluorochrome. (kromnigon.com)
  • This causes the fluorochrome to emit light. (kromnigon.com)
  • For example, green fluorochromes emit most light at a wavelength around 500-540 nanometer. (kromnigon.com)
  • Thereby fluorochromes continuously are excited and emit light when illuminated the excitation light source. (kromnigon.com)
  • This peak interval determines the color of the fluorochrome. (kromnigon.com)
  • The filter sets effectively separate seven classes of common fluorochromes, with less than 0.5% bleed through between any channels. (kromnigon.com)
  • Especially if the staining intensity varies between fluorochromes in the sample. (kromnigon.com)
  • How sample biology can influence fluorochrome choice. (labroots.com)