Fluorescent vitro and cell. Medical search. Frequent questions

Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (cdc.gov)
In vitro differentiation of small intestinal epithelium that approaches physiologic functionality of the in vivo host may allow for the development of a pathogenesis model for norovirus. (cdc.gov)
According to our findings the ex vivo proliferation capacity and functionality of NK cells is affected by multiple factors, such as the donor, composition of starting material, cytokine combination and the activation protocol. (frontiersin.org)
1. UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo. (nih.gov)
4. Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. (nih.gov)
14. In vivo cell biology of cancer cells visualized with fluorescent proteins. (nih.gov)
16. Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies. (nih.gov)
18. In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. (nih.gov)
20. In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases. (nih.gov)
This paper is based on the in vivo and in vitro study in glycation and lung cancer model systems. (hindawi.com)
Bioorthogonal covalent labeling with self-labeling enzymes like SNAP-tag bears a high potential for specific targeting of cells for imaging in vitro and also in vivo . (rsc.org)
Therefore, promising in vitro results are often not followed by respective success in vivo. (helsinki.fi)
IN VIVO ANGIOGENESIS ASSAY: CT26 murine colon carcinoma cells expressing green fluorescent protein were delivered into mouse dorsal skinfold window chambers. (duke.edu)
These results strongly circumscribe the possible mechanisms of PINK1 action in the mitochondrial life cycle and also raise the possibility that mitochondrial turnover events that occur in cultured embryonic axons might be restricted to the cell body in vivo, in the intact nervous system. (sdbonline.org)
Androgen receptor (AR) was detected in vivo by Western blotting and the cells responded to methyltrienolone (R1881). (nih.gov)
However, the green fluorescent protein (GFP) gene was introduced and stably expressed in PacMetUT1 cells, allowing tumor imaging in vivo. (nih.gov)
Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. (duke.edu)
The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration. (duke.edu)
An integrated view of supramolecularity implies that proteins crystallizing either in vitro or in vivo or participating in cellular processes share common attributes, notably determinants and antideterminants that favour or disfavour their correct or incorrect associations. (iucr.org)
Fluorescent reporter technologies are emergent tools to follow cellular processes in vivo [ 7 ]. (degruyter.com)

The use of the chromium-release assay to determine cytotoxicity of effector against target cells has various limitations mostly due to the inherent properties of the radioactive substance. (nih.gov)
These conditions were standardized by cell viability immunofluorescence assay (LIVE/DEAD), and cell invasion qualitative analysis (colorimetric method with acridine orange). (usp.br)
This in vitro assay employs the fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. (immunochemistry.com)
ICT's FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. (immunochemistry.com)
We probed a panel of proximal His mutants of cyanobacterial, human, and plant HO enzymes using a live-cell activity assay based on heterologous co-expression in Escherichia coli of each HO mutant and a fluorescent biliverdin biosensor. (rcsb.org)
Finally, Chapter 6 examines the interaction of the chaperone Hsp70 with an unfolding substrate using a FRET-based binding assay both in vitro and in cells. (illinois.edu)

GloFish is a patented technology which allows GM fish (tetra, barb, zebrafish) to express jellyfish and sea coral proteins giving the fish bright red, green or orange fluorescent colors when viewed in ultraviolet light. (wikipedia.org)
NK cells respond to stress signals such as NKG2D ligands [MHC class I chain-related proteins (MICA/MICB) and UL16 binding proteins (ULBP-1 to ULBP-6)], to nectins and nectin-like (Necls) molecules with DNAM-1 ( 3 , 4 ), or to an absence of self-antigens such as downregulation of HLA class I ( 5 - 8 ). (frontiersin.org)
3. Fluorescent proteins enhance UVC PDT of cancer cells. (nih.gov)
Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. (intechopen.com)
I am using fluorescent protein tagged virus proteins and a range of markers that label different cellular compartments to look at what happens during virus infection in live cells. (brookes.ac.uk)
Enhanced Apoptosis and Loss of Cell Viability in Melanoma Cells by Combined Inhibition of ERK and Mcl-1 Is Related to Loss of Mitochondrial Membrane Potential, Caspase Activation and Upregulation of Proapoptotic Bcl-2 Proteins. (immunochemistry.com)
In contrast to in vitro studies with purified proteins, we observed that multiple HO mutants retained significant activity within the intracellular environment of bacteria. (rcsb.org)
In fungi, monothiol glutaredoxins are central regulators of key cellular functions such as iron homeostasis, cell wall integrity and redox status via their interactions with other proteins as well as iron-sulfur clusters and glutathione. (ubc.ca)
Cellular distributions of the ERM proteins in MDCK epithelial cells: regulation by growth and cytoskeletal integrity. (embl-heidelberg.de)
The highly homologous ERM (ezrin/radixin/moesin) proteins, molecular cross-linkers which connect the cell membrane with the underlying cytoskeleton, have molecular weights of 81, 80 and 78 kDa respectively. (embl-heidelberg.de)
We present data which shows significant variation in the molecular weight and presence of multiple forms of ERM proteins in different cell lines, such that specific antibodies to each protein are essential for unambiguous detection. (embl-heidelberg.de)
Available open reading frames include a wide selection of transcription factors, TXTL modulators and fluorescent reporter proteins to build multi-stage gene circuits. (biocat.com)
Project title: Possible role of membrane contact sites between the endoplasmic reticulum (ER) and lysosomes in resistance to chemotherapeutics in breast cancer cell models modulated by key ER proteins. (faseb.org)
Focusing on this topic in the hemo-lymphoid system, we have identified the Ikaros gene family of zinc finger DNA binding proteins and shown in a series of genetic studies that they function as key regulators of lymphocyte specification working from the level of the hemopoietic stem cell (HSC). (massgeneral.org)
conducted a series of experiments to identify specific proteins in the melanoma cellsthat were responsible for making the cancer more invasive after it interacted with the lymph vessel cells. (elifesciences.org)
These experiments identified proteins called MMP14, Notch3, and β1-integrin as critical to the invasive spread of melanoma cells. (elifesciences.org)
This work includes efforts to better understand the folding of model proteins in vitro, with an eye towards how this insight can inform questions about protein dynamics in more complex environments and protein-protein interactions. (illinois.edu)
Chapter 1 is a survey of the theory of protein folding and the major techniques and findings from the study of fast folding proteins in vitro, with a special emphasis on how this work informs our understanding of more complex protein dynamics. (illinois.edu)
Although some of these molecules act by binding to cytoplasmic receptors, many of them act by binding to specific cell surface receptors, some of which are subsequently internalized as a complex, while others act by transmembrane signalling of their binding via membrane proteins coupled to GTP binding proteins or polyphosphoinositide--diacylglycerol generation. (cuanschutz.edu)
It is of particular interest where on the cell-surface interacting human proteins are located and what their binding partners are. (degruyter.com)

Microscopy, PCR, and fluorescent in situ hybridization provided evidence of norovirus infection. (cdc.gov)
As a functional measure of gap junctional communication, donor cells were microinjected with the fluorescent dye, Lucifer yellow, and the transfer of dye from donor cells to primary neighbor cells was monitored by fluorescence microscopy. (nih.gov)
Acutely prepared slices of neonatal rat cerebellum in conjunction with confocal microscopy and fluo4 epifluorescence were used to track changes induced by MeHg in CGC Ca(2+) i regulation in the external (EGL) and internal granule cell layers (IGL) as well as the molecular layer (ML). MeHg caused no cytotoxicity but did cause a time-dependent increase in fluo4 fluorescence that depended on the stage of CGC development. (nih.gov)
Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. (immunochemistry.com)
They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. (intechopen.com)
I also use electron microscopy to uncover how the host cell changes during viral infection in high resolution. (brookes.ac.uk)
Here, we describe the use of serial block-face scanning electron microscopy to achieve high-resolution 3D characterisation of P10 structures within Trichoplusia ni TN-368 cells infected with Autographa californica multiple nucleopolyhedrovirus. (brookes.ac.uk)
Microscopy based on two-photon excitation of fluorescent VSDs offers the possibility of recording sub-millisecond membrane potential changes on micron length scales in cells that lie upwards of one millimeter below the brain's surface. (springer.com)
To this end, fluorescent SNAP substrates have been established and used in microscopy and fluorescence imaging while radioactive substrates for the highly sensitive and whole-body positron emission tomography (PET) have been lacking. (rsc.org)
The use of labels is common in traditional biochemical and cell culture assays as well as in fluorescence microscopy, however the main drawback of this approach is that derivatization with fluorescent probes can change the behavior and properties of drug molecules or drug delivery systems, or even cell behavior. (helsinki.fi)
RESOLFT is a similar technique that employs fluorescent molecules with 'on' and 'off' times that are much longer than those used in STED microscopy. (elifesciences.org)
In addition, recent advances in NMR, mass spectrometry, fluorescence microscopy, and other techniques have enabled the study of protein folding in the cellular environment and have shown that the effect of the cell on protein folding is variable and difficult to predict. (illinois.edu)
Chapter 4 is a practical guide to improving the accuracy and reliability of Fluorescence Relaxation Imaging (FReI), our Fӧrster Resonance Energy Transfer (FRET) microscopy based technique for studying biomolecular kinetics in the cell. (illinois.edu)
Alkyne functionalized dyes were used for bioorthogonal click reactions by labeling of metabolically incorporated sugar-azides on the surface of living neuroblastoma cells, which were applied to direct stochastic optical reconstruction microscopy ( d STORM) for the visualization of cell-surface glycans in the nm-range. (degruyter.com)
These limitations can be overcome using small covalently attached fluorescent probes with short linkers in combination with new super-resolution fluorescence microscopy methods [ 9 ], [ 10 ]. (degruyter.com)

The image is build up by scanning both lasers over the sample so that the small region in which the fluorophores are 'on' probes the entire cell. (elifesciences.org)
Furthermore, fluorescent probes with high selectivity and sensitivity are developed to characterize Lp-PLA2 and its enzymatic activity in vitro or even in living cells in a way more convenient than immunoblotting tests or immunofluorescence imaging. (proteopedia.org)

Two species of fish, zebrafish (Danio rerio) and medaka (Japanese rice fish, Oryzias latipes), are most commonly modified because they have optically clear chorions (shells), develop rapidly, the 1-cell embryo is easy to see and micro-inject with transgenic DNA, and zebrafish have the capability of regenerating their organ tissues. (wikipedia.org)
Using models derived from human cells and tissues, called in vitro models, researchers designed a 3D organoid model that mimics how the human heart develops. (nih.gov)
Targeted pulsed focused ultrasound (pFUS) increases stem cell homing to tissues by establishing a trophic microenvironment through calcium-dependent cyclooxygenase-2 (COX2) signaling. (nih.gov)
The precipitation of AGEs in various cells, tissues, and organs is one of the factors for the initiation and progression of various metabolic derangements including the cancer. (hindawi.com)
The research community needs tools that can probe living cells in real tissues to produce high dimensional time-series data from cells of interest. (nih.gov)
Although we cannot guarantee that an antibody will work in all cells or tissues, under all conditions, our validation shows that the antibody recognizes the fixed antigen. (rndsystems.com)
Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. (duke.edu)

Fluorescent ionophore imaging revealed both intracellular Ca2+ and Na+ transients during pFUS. (nih.gov)
Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors NANOSCALE. (nottingham.ac.uk)

We have developed an improved flow cytometric method that is able to measure cytotoxicity, based on two fluorescent dyes. (nih.gov)
It is shown that in comparison with organic dyes, quantum dots have superior photostability of tracking apoptosis in cancer cells for longer time. (intechopen.com)
Herein we report the feasible quantitative scale synthesis of photoswitchable alkyne functionalized water-soluble cyanine dyes with absorption wavelengths from ~550 (m=1) to ~650 nm (m=2) which have been used successfully for the bioorthogonal Huisgen-Meldal-Sharpless click reaction on the surface of living cells. (degruyter.com)

Furthermore, I had the opportunity to be a part of a collaboration with biophysicists, which led to the development of a single-molecule fluorescent in situ hybridization (smFISH)-based M ultiplex S patial T ranscriptome A nalysis (scMST) that revealed a pluripotent-like neural crest stem cell domain in the dorsal neural tube. (nih.gov)

The method is used to quantify natural killer (NK) and lymphokine-activated killer (LAK) activities against the human K562 and Daudi cell lines and the murine YAC-1 and L1210 cell lines respectively, as well as cell-mediated lympholysis (CML) exerted by tumor-infiltrating lymphocytes (TIL) against autologous and allogeneic human breast cancer tumor cells. (nih.gov)
Since they possess natural mechanisms to recognize and kill tumor cells, NK cells are considered as a potential option for an off-the-shelf allogeneic cell-based immunotherapy. (frontiersin.org)
The success of this first project led McAlpine and his collaborators to tackle another project, this time by combining these 3D printed biochemical capsules with 3D bioprinted tumor cells. (nih.gov)
Schematic of the 3D bioprinted in vitro tumor model demonstrating the integration of the tumor cells, blood-vessel-like structures, and chemical gradients. (nih.gov)

Monolayers were subsequently washed and incubated with medium alone or containing the pro-inflammatory cytokine IL-1β (5 ng/mL) for 6 h to upregulate pro-atherosclerotic adhesion molecules (AM). AM expression was assayed by ELISA and binding of U937 human monocytes pre-loaded with fluorescent dye was determined. (biomedcentral.com)
Real- time measurements on the spatial and temporal fluctuations of single molecules in living cells, which are not possible using other methods, are a major goal of this initiative. (nih.gov)
Exosomes contain cargo material from endosomes, cytosol, plasma membrane and microRNA molecules, they are released by a number of non-cancer and cancer cells into both the extracellular microenvironment and body fluids such as blood plasma. (brookes.ac.uk)
This approach involves tagging the biomolecules of interest with fluorescent molecules, such as green fluorescent protein (GFP), so that they can be identified in cells. (elifesciences.org)
In particular, RESOLFT uses fluorescent molecules that can be rapidly switched back and forth between long-lived 'on' and 'off' states many times by the two lasers. (elifesciences.org)
By labeling microtubules and the drugs with fluorescent molecules, we can follow how microtubules are formed and what happens when the drug binds to them", says the last author Anna Akhmanova of Utrecht University. (uu.nl)

One of our main research aims is to develop novel complementary real-time label-free in vitro tools for existing static biological assays and animal tests in the field of preclinical drug research and drug discovery. (helsinki.fi)
Current functional in vitro biochemical and cell culture assays used in pharmaceutical and life science research are static and only shed light on the endpoint equilibrium. (helsinki.fi)
Thus, there is a clear need for real-time label-free biomimetic in vitro assays that allows one to monitor, detect and distinguish between drug and nanoparticle induced cellular processes, such as target specific drug induced cell signaling pathways and cell uptake mechanisms. (helsinki.fi)

I gained my PhD in plant cell biology from Oxford Brookes University in 2004, which was provided great training in different imaging techniques which I continue to use today. (brookes.ac.uk)
I received my Ph.D. in stem cell biology from the Faculty of Medicine at The University of Helsinki, Finland. (nih.gov)
Cavieres is a postdoctoral fellow in Patricia Burgos' lab at the Center for Cell Biology and Biomedicine at the University of San Sebastián in Providencia, Chile, where she studies the potential involvement of endoplasmic reticulum quality control in resistance to chemotherapy for breast cancer. (faseb.org)
Mammalian cell biology is dynamic, but today's 'omic approaches require destruction of cells to access their molecular contents and cannot resolve the full richness of cellular diversity in situ . (nih.gov)

Fluorescence Methods for Investigation of Living Cells and Microo. (intechopen.com)
The winners will share $400,000 in prizes awarded for the development of new tools and methods for predicting the behavior and function of a single cell in complex tissue - and how that reflects the health of the tissue. (nih.gov)
Phase 1 of the Follow that Cell Challenge sought novel robust methods for analysis of individual cells to detect and assess changes in cell behavior and function over time either as a result of natural state changes or when perturbed. (nih.gov)
Now, in addition to single-cell sequencing technology, we have integrated methods to monitor temporal changes in the phosphorylation and activation of transcription factors. (nih.gov)
METHODS: Ang2-mediated Tie2 receptor phosphorylation was assessed in vitro in the absence and presence of aptamer coupled to polyethylene glycol. (duke.edu)
Chapter 3 is an introduction to how perturbative methods developed in vitro can be applied to study processes in the cell that are inaccessible by conventional steady-state measurements. (illinois.edu)

The basic task of the fluorescence microscope is to permit excitation light to irradiate the specimen and then to separate the much weaker re-radiating fluorescent light from the brighter excitation light. (fsu.edu)
An excitation laser then drives the fluorescent molecule, which is also known as a fluorophore, into an excited state: after a short time, the fluorophore can return to its ground state by releasing a fluorescence photon. (elifesciences.org)

J Cell Biol (2005) 169 (5): 719-724. (rupress.org)

Induced pluripotent stem cells (iPSCs) have recapitulated several aspects of Parkinson's disease (PD), but most iPSCs are derived from familial cases, which account for only about 15% of patients. (plos.org)
PD ranks as the second most common neurodegenerative disease after Alzheimer's disease [ 1 ], and patient specific induced pluripotent stem cells (iPSCs) have shown great promise in recapitulating critical aspects of PD pathogenesis, enabling mechanistic studies as well as the identification of novel therapeutics [ 2 ]. (plos.org)
2D model showing how the pluripotent stem cells react to human relevant doses of cadmium over 8 days. (nih.gov)
First, they used human pluripotent stem cells to develop 3D embryoid bodies to mimic early steps in tissue and organ formation in humans. (nih.gov)

Fluorescence imaging allows researchers to visualize these cancer cells migrating through the constructed blood vessels upon release. (nih.gov)

The Assessment of the Phototoxic Action of Chlortetracycline and Doxycycline as a Potential Treatment of Melanotic Melanoma-Biochemical and Molecular Studies on COLO 829 and G-361 Cell Lines. (immunochemistry.com)
We found that the human FBR is mediated by immune-cell-specific RAC2 mechanotransduction signalling, independently of the chemistry and mechanical properties of the implant, and that a pathological FBR that is human-like at the molecular, cellular and tissue levels can be induced in mice via the application of human-tissue-scale forces through a vibrating silicone implant. (stanford.edu)
We have developed a novel nanopipette technology that can be used to monitor the molecular properties of single cells over time. (nih.gov)
These fundamental studies continue to contribute insight into the earliest steps of the B and T cell differentiation pathways and the molecular network that supports cell fate decisions in the hemo-lymphoid system. (massgeneral.org)
To dissect the underlying molecular mechanisms, we established LEC co-cultures with different melanoma cells originating from primary tumors or metastases. (elifesciences.org)
We like to use special synthesized carbohydrate labels, which, in combination with new super-resolution imaging, allow the identification of cell-surface glycans well below the diffraction limit approaching virtually molecular resolution. (degruyter.com)

BACKGROUND: No rabies post-exposure prophylaxis (PEP) failure has been documented in humans in the United States using modern cell-culture vaccines. (cdc.gov)

My graduate work focused on characterization of regulatory functions in mouse neural stem cells. (nih.gov)
Several monoclonal cell strains with a normal karyotype and gene expression profile upon characterization have been screened out from the untransfected HCE cell line by limited dilution techniques in our laboratory [ 18 ], prompting us to hypothesize that these cells can be used for TE-HCE reconstruction and clinical transplantation trial as well. (molvis.org)
Characterization of PacMetUT1, a recently isolated human prostate cancer cell line. (nih.gov)
Chapter 5 describes the development and characterization of the fluorescent construct GPGK-tc and its use to study population-level variation of protein folding in E. coli. (illinois.edu)

8. Wavelength dependence of cellular responses in human melanocytes and melanoma cells following exposure to ultraviolet radiation. (nih.gov)
We show here that exposure of expansively growing human WM852 melanoma cells, but not singly invasive Bowes cells, to lymphatic endothelial cells (LEC) in 3D co-culture facilitates melanoma distant organ metastasis in mice. (elifesciences.org)
Notably, the expansively growing metastatic melanoma cells adopted an invasively sprouting phenotype in 3D matrix that was dependent on MMP14, Notch3 and β1-integrin. (elifesciences.org)
investigated whether exposing human melanoma cells to cells from the walls of human lymph vessels would make the cancer cells more aggressive. (elifesciences.org)
Indeed, after growing the two cell types together in the laboratory, the melanoma cells became more invasive. (elifesciences.org)
When melanoma cells with less MMP14 or Notch3 were implanted into zebrafish, the cancer cells spread less efficiently. (elifesciences.org)

The expansion conditions were evaluated based on NK cell viability, functionality, expansion rate and purity. (frontiersin.org)
Since 1994, ImmunoChemistry Technologies has been producing top quality reagents, kits, and services for cell viability and ELISA applications in a broad range of research areas. (immunochemistry.com)
5. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area. (nih.gov)

The uncontrollable scratching as caused by pruritus promotes the release of TSLP from keratinocytes, which regulates the differentiation of T cells into T H 2 type, producing a large amount of T H 2 cytokines (e.g. (nature.com)
The effect of BPA-G on the differentiation of human and 3T3L1 murine preadipocytes was evaluated in vitro by quantifying lipid accumulation and the expression of adipogenic markers. (nih.gov)
From the control in the first panel, to the last panel, researchers can see how the differentiation to cardiomyocytes (as shown by the green fluorescent-positive cells) is inhibited with different doses of cadmium. (nih.gov)
My research interest is focused on the mechanisms by which cell de-differentiation and cell identity are regulated. (nih.gov)
Loss of function of Ikaros' chromatin remodeling associates in the skin interferes with the maintenance and differentiation of epidermal and follicular stem cells in unexpected ways. (massgeneral.org)
Both aspects of normal development and neoplastic transformation are studied here using state-of-the-art mouse genetic models, cell isolation and differentiation systems combined with comparative global transcriptome and epigenome approaches. (massgeneral.org)
This combination of attributes makes Ikaros an ideal entry point to study epigenetics in stem cell commitment and differentiation. (massgeneral.org)

As a postdoc in the Kerosuo lab, I hope to expand my neural crest and craniofacial research portfolio with a project exploring gene expression signatures that occur across the dorsal and ventral neural tube of the chick to better understand the specific function of genes known to be necessary for neural crest cell specification and survival. (nih.gov)
Initially, we developed the technology to monitor changes in gene expression (genomics) in single cells. (nih.gov)
This allows us to monitor the activation of different transcription factors and the array of genes each one regulates to investigate how different signaling pathways interact in controlling gene expression in a single cell. (nih.gov)
In many developmental systems, nuclear regulators have been implicated in coupling key events in gene expression with specific cell fate and lineage decisions. (massgeneral.org)
Since the untransfected HCE cell line exhibited chromosomal aneuploidy, monoclonal HCE cell strains with normal diploid karyotypes and gene expression profile are believed to be the ideal seeder cells for TE-HCE reconstruction. (molvis.org)
Many of these mRNAs occur less than one copy per every ten cells, suggesting a high degree of cellular specificity in neural gene expression. (cuanschutz.edu)

My postdoctoral (postdoc) work in Professor Marianne Bronner's laboratory demonstrated that neural crest cells, despite their transient nature, maintain a premigratory stem cell niche of self-renewing and pluripotent cells located in the dorsal neural tube. (nih.gov)

The researchers saw how exposure to low levels of cadmium can block usual formation of cardiomyocytes, which are the major type of cells that form the heart. (nih.gov)
They then used a 2D in vitro model that included a fluorescent regulatory protein system (NKX2-5) known to be involved in heart development, which allowed them to look at cadmium toxicity after exposure. (nih.gov)
2022. Cardiac Development in the Presence of Cadmium: An in vitro Study Using Human Embryonic Stem Cells and Cardiac Organoids. (nih.gov)
The images demonstrate some of the different cell types in the organoids and specific markers the team used to see how cadmium suppresses the organoid. (nih.gov)

Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (cdc.gov)
The aim of this study was to in vitro evaluate the direct and indirect effect of viable C. albicans on the human palatal epithelial cells (HPEC) over time. (usp.br)
The glomerular epithelial cell anti-adhesin podocalyxin associates with the actin cytoskeleton through interactions with ezrin. (embl-heidelberg.de)
During development, renal glomerular epithelial cells (podocytes) undergo extensive morphologic changes necessary for creation of the glomerular filtration apparatus. (embl-heidelberg.de)

long lasting probe for cell migration tracking? (bio.net)
Arsenic-based Nrf2 activators, fluorescent biarsenical labeling reagent (FlAsH) and phenylarsine oxide (PAO), were used to probe binding of arsenic to Keap1. (cdc.gov)
Ataka K, Pieribone VA (2002) A genetically targetable fluorescent probe of channel gating with rapid kinetics. (springer.com)
Clarke RJ, Zouni A, Holzwarth JF (1995) Voltage sensitivity of the fluorescent probe RH421 in a model membrane system. (springer.com)
Dragsten PR, Webb WW (1978) Mechanism of the membrane potential sensitivity of the fluorescent membrane probe merocyanine 540. (springer.com)

Moesin is a member of the ERM family and is involved in plasma membrane-actin cytoskeleton cross-linking, resulting cell adhesion, shape, and motility. (embl-heidelberg.de)

Other transgenic lines of pet fish include Medaka which remain transparent throughout their lives and pink body color transgenic angelfish (Pterophyllum scalare) and lionhead fish expressing the Acropora coral (Acroporo millepora) red fluorescent protein. (wikipedia.org)
15. Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis. (nih.gov)
Visit our Cell-free Protein Expression page to learn more about the myTXTL System . (biocat.com)
While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. (elifesciences.org)
have modified this protein to make a new fluorophore called rsEGFP2 with a shorter switching time, and have used it to image various structures-including Vimentin, a protein that forms part of the cytoskeleton in many cells, and organelles called peroxisomes-inside live mammalian cells. (elifesciences.org)
Attention has now turned to developing a better understanding of how protein structure, function, and folding interact to enable necessary biochemical processes in the cell. (illinois.edu)
This thesis is roughly divided into two sections: Chapters 1-2 discuss fast protein folding in vitro and Chapters 3-6 address the study of protein folding and folding mediated processes in the cell. (illinois.edu)

The filtered UV light illuminates the specimen, in this case a crystal of fluorspar, which emits fluorescent light when illuminated with ultraviolet light. (fsu.edu)

Here, our objective was to identify the optimal cytokine-based, feeder-free, activation and expansion protocol for cytotoxic NK cells against glioblastoma in vitro . (frontiersin.org)
Glioblastoma (GBM) is a highly aggressive brain tumor originating from the nerve cell-supporting astrocytes. (frontiersin.org)

We have, for several years, been developing real-time label-free platforms based on surface plasmon resonance in combination with living cell sensing and quartz crystal microbalance in combination with lipid bilayers as cell model layers for assessing drug and nanoparticle targeting and delivery efficacy. (helsinki.fi)

The FLICA reagent SR-LEHD-FMK enters each cell and irreversibly binds to activated caspase-9. (immunochemistry.com)
Because the SR-LEHD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound SR-LEHD-FMK FLICA reagent diffuses out of the cell and is washed away. (immunochemistry.com)
The remaining red fluorescent signal is a direct measure of the active caspase-9 enzyme activity present in the cell at the time the reagent was added. (immunochemistry.com)
Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. (immunochemistry.com)

We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. (nih.gov)

Additionally, the hydrogels contain cores that are packed with chemicals to guide cancer cell migration or blood vessel growth, plus an encapsulating outer shell comprised of gold nanorods. (nih.gov)
It acts on microtubules, cellular filaments that are involved in cell division and cell migration. (uu.nl)

7. Effect of ultraviolet irradiation on MCA102 tumor cell immunogenicity and sensitivity to tumor necrosis factor. (nih.gov)

X-ray crystallographic structures of human HO1 H25R with bound heme and additional functional studies suggest that HO mutant activity inside these cells does not involve heme ligation by a proximal amino acid. (rcsb.org)

Human aortic endothelial cells (HAEC) were incubated overnight with control media with dimethylsulfoxide (DMSO) vehicle (1% v/v) or containing DMSO extracts of whole dehydrated mushrooms (0.1 mg/mL), which included Agaricus bisporus (white button and crimini), Lentinula edodes (shiitake), Pleurotus ostreatus (oyster), and Grifola frondosa (maitake). (biomedcentral.com)
Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (cdc.gov)
Understanding of the pathogenesis of human noroviruses has been limited by our inability to propagate these viruses in vitro ( 12 ). (cdc.gov)
However, these models do not answer the fundamental questions of human norovirus attachment and entry into cells of the human gastrointestinal tract. (cdc.gov)
Representative models of differentiated human intestinal epithelium can be established by growing cells in 3 dimensions (3-D) on collagen-I-coated porous microcarrier beads in rotating-wall vessel (RWV) bioreactors that model the physiologic fluid-shear environment in their respective organs ( 21 - 24 ). (cdc.gov)
NK cells were enriched from human peripheral blood and expanded for 16 days with different activation and cytokine combinations. (frontiersin.org)
9. Differential apoptotic pathways in human keratinocyte HaCaT cells exposed to UVB and UVC. (nih.gov)
Human TH17 cells engage gasdermin E pores to release IL-1α on NLRP3 inflammasome activation. (immunochemistry.com)
While the proposed studies focus on the use of our nanopipette technology to study human cancer cells in culture, the cell monitoring capabilities of the nanopipette allow it to be used for in situ analysis of single cells in tissue biopsies and slices. (nih.gov)
Human corneal endothelial (HCE) cells of the innermost layer of the cornea play vital roles in maintaining corneal transparency by barrier and pump functions. (molvis.org)
They are round, half as thick as a human hair, contain gold nanorods and fluorescent dye, and are surrounded by a biomaterial obtained from algae. (tum.de)
They are currently being used in vitro , outside the human body. (tum.de)

We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations. (nih.gov)
I then continued as a postdoctoral fellow at the California Institute of Technology where I initiated my research on stem cell characteristics of the neural crest by adopting the early chicken embryo as my model system. (nih.gov)
I made this discovery by creating novel techniques such as developing crestospheres, an in vitro method to maintain neural crest stem cells in a self-renewing state. (nih.gov)
Precise understanding of the mechanisms that induce and maintain the pluripotency of the stem cell niche in neural crest cells is fundamental to model neural crest defects to develop regenerative therapeutic strategies. (nih.gov)
My broad research interest lies in the mysteries of how a stem-cell has the potential to become nearly any cell in the body. (nih.gov)
Nanosensors capable of measuring pH, oxygen, glucose, calcium, zinc, temperature and enzyme activity have been demonstrated and they have been applied to a range of biological systems including nematodes, stem cells, cancer spheroids, yeast cells and bacterial biofilms. (nottingham.ac.uk)

When HCE cells are damaged due to various causes (e.g., viral infection, intraocular surgery, or Fuchs dystrophy), corneal endothelial dysfunction occurs. (molvis.org)

Preimplantation biopsy of blastocysts obtained by in vitro fertilization is an invasive technique. (medscape.com)
PacMetUT1 cells are more invasive in Matrigel than DU-145, PC-3, and LNCaP cells, and showed greater anchorage-independent growth in soft agar. (nih.gov)

The researchers designed the hydrogel to mimic the composition and stiffness of physiological structures to allow cells to behave in a similar manner outside of the body. (nih.gov)
The National Institutes of Health has named two biological engineering researchers as winners in Phase 2 of its Follow that Cell Challenge. (nih.gov)
The combination of RESOLFT and rsEGFP2 should allow researchers to image a wide variety of structures and processes in living cells that have not been imaged before. (elifesciences.org)
But researchers also suspect that vessel cells interact with the cancer cells, helping the melanoma invade distant organs. (elifesciences.org)
A group of researchers at the Technical University of Munich (TUM) has developed the world's first microrobot ("microbot") capable of navigating within groups of cells and stimulating individual cells. (tum.de)

Microtubules in the cell, with fluorescent markers at the tips. (uu.nl)
Growth and shortening of microtubules, with fluorescent markers at the tips. (uu.nl)

Results from the UMN study, published in Advanced Materials, found that in the 3D bioprinted model, it takes drugs more time to kill fewer cells than previous studies have shown, but the results likely offer a more accurate representation of the body's processes than 2D drug screening platforms. (nih.gov)
This study examined whether the highly conserved cytoplasmic tail of podocalyxin also contributes to the unique organization of podocytes by interacting with the cytoskeletal network found in their cell bodies and foot processes. (embl-heidelberg.de)
Long threadlike processes (axons and dendrites) may stretch a meter or more from the cells nucleus, making highly specific synaptic connections with other cells. (cuanschutz.edu)
Cell-surface glycans have been shown to participate in cell-cell recognition processes and tumor development. (degruyter.com)

Calcein-AM, a non-fluorescent substance which is intracellularly converted to the green fluorescent calcein by esterase activity in viable cells, is initially used to stain target cells. (nih.gov)
Dead target cells are distinguished by their double (green-red) staining. (nih.gov)

This His-heme interaction has been regarded as being essential for enzyme activity, because His-to-Ala mutants fail to convert heme to biliverdin in vitro. (rcsb.org)

Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells. (immunochemistry.com)
Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells. (immunochemistry.com)

The cytotoxicity of these memory-like NK cells was enhanced with re-activation, diminishing the donor variation. (frontiersin.org)

2001) Quantitative comparison of algorithms for tracking single fluorescent particles. (mindmeister.com)

Recently, the first in vitro norovirus cell culture model was reported for a virus that infects mice ( 18 , 19 ). (cdc.gov)
I think our model has the key components to push the boundaries of in vitro cancer research. (nih.gov)
In this new model, the drug diffuses through the 3D system before it reaches its intended target, the cancer cells. (nih.gov)
The PacMetUT1 cell line allows metastases to be assessed using a single animal model. (nih.gov)

Cell 176 , 805-815.e8 (2019). (nature.com)

To establish the utility of this multiplexed technology, we are employing it to study a critical biological question: How do cancer cells develop drug resistance? (nih.gov)

Biochemical fractionation of MDCK cells demonstrates that although the individual ERM fractionation patterns are unaltered by cell density, the multiple forms of moesin each associate with different subcellular fractions. (embl-heidelberg.de)

TE-HCE reconstruction was performed by culturing 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-labeled monoclonal HCE cells on denuded amniotic membranes (dAMs) in 20% fetal bovine serum-containing Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F12 (1:1) medium and 5% CO 2 at 37 °C on a 24-well culture plate. (molvis.org)

20 ) reported production of Norwalk virus particles (norovirus GI.1, the prototype strain of this genera from Norwalk, Ohio) after transinfection of cultured kidney cells. (cdc.gov)

Thus, extensive dye transfer was observed in the rat myometrial smooth muscle cells under all culture conditions examined, regardless of sexual maturity or gestational stage of the animal, in vitro hormone treatment, or cell density. (nih.gov)
The reconstructed TE-HCE at a density of 3,413.33±111.23 cells/mm 2 in average established intense cell-cell and cell-dAM junctions. (molvis.org)
this was similar to the normal eye control in cell density. (molvis.org)

Co-senior author Angela Panoskaltsis-Mortari, Ph.D., the Vice Chair for Research and Professor in the Department of Pediatrics at UMN, said, "Blood vessels help cancer cells migrate to other sites in the body, a process called metastasis, which complicates treatment strategies. (nih.gov)
Cancer cells invade the blood vessel-like structures due to the guided growth created by the chemical gradients, thereby mimicking metastasis. (nih.gov)
Three-dimensional bioprinting technology was used to precisely place melanoma or lung cancer cells, normal cells, and blood vessel-like structures in the laboratory dish based on their normal functions. (nih.gov)
She investigated the role of the GOLPH3 oncoprotein in the function of lysosomes in breast cancer cells, mentored by Gonzalo Mardones. (faseb.org)
This is beneficial for the fight against cancer cells: the ability of cancer cells to divide and migrate is inhibited, which limits tumor growth and metastases. (uu.nl)
When transplanted into mice, these cancer cells spread to and invaded the rodents' distant organs. (elifesciences.org)

Vivo20

Assay6

Proteins20

Microscopy15

Probes2

Tissues7

Intracellular2

Dyes3

Hybridization1

Tumor cells4

Molecules6

Assays3

Biology4

Methods6

Excitation2

Biol1

Pluripotent stem4

Visualize1

Molecular6

Humans1

Characterization4

Melanoma cells6

Viability3

Differentiation7

Gene6

Dorsal1

Cadmium4

Epithelial4

Probe5

Motility1

Protein7

Emits1

Glioblastoma2

Lipid1

Reagent4

Reporter1

Migration2

Sensitivity1

Functional1

Human12

Stem6

Endothelial1

Invasive2

Researchers5

Markers2

Processes4

Green2

Interaction1

Apoptosis2

Cytotoxicity1

20011

Model4

20191

Biological1

Biochemical1

Fetal1

Particles1

Density3

Cancer cells6