• Fragmented nucleic acid sequences of target, labelled with fluorescent dyes. (wikipedia.org)
  • Membrane probes include fluorescent analogs of natural lipids, as well as lipophilic organic dyes that have little structural resemblance to natural biomolecules ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
  • The fluorescent dyes provided with WesternBright MCF and WesternBright MCF-IR outperform Cy™ dyes and allow detection of low pg of protein. (thomassci.com)
  • Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points. (rsc.org)
  • Detection of PCR products in real-time can be accomplished by using fluorescent dyes or probes. (cdc.gov)
  • Fluorescently-labeled probes detect the amount of specific double-stranded DNA sequences while fluorescent dyes detect only the amount of double-stranded DNA. (cdc.gov)
  • Fluorescent indicators for Ca 2+ based on green fluorescent proteins and calmodulin. (nature.com)
  • Baird, G. S., Zacharias, D. A. & Tsien, R. Y. Circular permutation and receptor insertion within green fluorescent proteins. (nature.com)
  • For example, tumors may evade immune detection by manipulating the expression of immune checkpoint proteins (e.g. (cellsignal.com)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • WesternBright MCF visible and near infrared (IR) fluorescent Western blotting kits allow the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. (thomassci.com)
  • Fluorescent sensor proteins are instrumental for detecting biological signals in vivo with high temporal accuracy and cell-type specificity. (biorxiv.org)
  • The detection and evaluation of concentration of influenza virus proteins in biological samples is critical in a broad range of medical and biological investigations regarding the concern over potential outbreaks of virulent influenza strains in animals and humans. (omicsonline.org)
  • This parallel detection of PB1-F2 and NP suggests that applied sensor chip technology may be amenable to an arrow immunosensor for simultaneous detection of all known influenza virus proteins in infected tissues and cells. (omicsonline.org)
  • Oligonucleotide probes are increasingly being used to characterize natural microbial assemblages by Tyramide Signal Amplification-Fluorescent in situ Hybridization (TSA-FISH, or CAtalysed Reporter Deposition CARD-FISH). (hal.science)
  • The plasma membranes and intracellular membranes of live cells and the artificial membranes of liposomes represent a significant area of application for fluorescent probes. (thermofisher.com)
  • and for investigating synaptosome recycling ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ) and lipid-mediated signal transduction processes ( Probes for Signal Transduction-Chapter 17 ). (thermofisher.com)
  • Fatty acids are liberated by the enzymatic action of phospholipase A on phospholipids ( Probes for Lipid Metabolism and Signaling-Section 17.4 ) and also by various other lipases. (thermofisher.com)
  • So far, many selective smart-molecular fluorescent probes targeting Zn 2+ have been reported, that are mostly based on quinoline, fluorescein, benzazole, or fluorophores [ 6 - 10 ]. (hindawi.com)
  • Over the past decade, the modification of small molecular fluorescent probes on the surface of nanostructured materials has been attracting significant attention [ 15 - 18 ]. (hindawi.com)
  • Thus HRP-catalyzed deposition of tyramide-fluorophore molecules provides enhanced amplification of fluorescence signal at the site of the antigen. (cellsignal.com)
  • Light produced by a system of few optically active biomarkers (fluorescent molecules, quantum dots) exhibits quantum behavior. (nist.gov)
  • Those runaway scissors could be used for diagnostic tests: Once they're triggered to start cutting by the presence of, say, a specific germ, the continued cutting can also turn on fluorescent molecules that signal "Germ detected! (wbur.org)
  • Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. (uni-muenchen.de)
  • The fluorescence signal increases at each PCR cycle as more double-stranded DNA molecules are generated. (cdc.gov)
  • The kits provide high levels of specificity, sensitivity (see figure " Highly sensitive detection of HBV DNA. Highly sensitive detection of HBV DNA. "> Highly sensitive detection of HBV DNA "), and reproducibility over a broad linear range. (qiagen.com)
  • Tissue autofluorescence can be an issue often contributing to poor sensitivity in fluorescent IHC. (cellsignal.com)
  • Preliminary experiments permitted to determine the device sensitivity in the μmol.L -1 range as regards the detection of PBV fluorescence signals. (spie.org)
  • The instrument employs the spectral detector GaAsp that allows for high sensitivity detection of 32-channels and three additional detectors (PMTs), two for confocal detection and one for transmitted light detection. (lu.se)
  • The interaction of biotin and Avidin or Streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. (genomicglossaries.com)
  • Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. (vectorlabs.com)
  • The results show that the fluorescence signal intensity distribution is fairly narrow indicating high chemical reaction uniformity among the beads population. (cdc.gov)
  • However, conventional optical codes based on fluorescent color and intensity offer a limited number of possible codes. (chemistryviews.org)
  • As the PCR progresses and the quantity of double-stranded DNA increases, more dye binds to the PCR products and hence, signal intensity increases. (cdc.gov)
  • A confocal scanner then reads the fluorescent intensity of each hybridized sequence in the array. (medscape.com)
  • Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
  • To support this spectrum of requirements, BD Biosciences offers a full range of apoptosis detection tools and technologies for measuring indicators at different stages across the apoptotic process. (bdbiosciences.com)
  • Sphingolipids play critical roles in processes such as proliferation, apoptosis, signal transduction and molecular recognition at cell surfaces. (thermofisher.com)
  • TUNEL based assay for high throughput quantitative detection of apoptosis. (rndsystems.com)
  • Why Use the TiterRACS In Situ Apoptosis Detection Kit? (rndsystems.com)
  • The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. (rndsystems.com)
  • Detection of Apoptosis in Staurosporine-treated ML-1 Cells. (rndsystems.com)
  • All necessary reagents optimized for reliable HBV DNA detection and quantitation, including genotypes A-H for in vitro diagnostic use, are provided. (qiagen.com)
  • artus HBV PCR Kits provide all necessary reagents optimized for reliable HBV DNA detection and quantitation. (qiagen.com)
  • Fluorescent tags-bound directly to the analyte-are easily disposable, but these reagents are not always sensitive enough to meet a researcher's needs ( J. Kling, The Scientist , April 14, 1997, page 14 ). (the-scientist.com)
  • The kit comes complete with the detection reagents required to measure activity. (bellbrooklabs.com)
  • Perform your enzyme reaction, add the detection reagents, and measure. (bellbrooklabs.com)
  • The processed sample is added to the Priming Microwell, which contains the amplification primers, fluorescent labeled detector probe, and other reagents necessary for amplification. (cdc.gov)
  • 13 ] synthesized a new fluorescent chemosensor based on a helical imide as fluorophore and a cyclen moiety as ionophore, which not only showed enhanced fluorescent responses in the presence of Zn 2+ , Cd 2+ , and Hg 2+ , but also could simultaneously and selectively distinguish the three cations in a simulated physiological condition with the help of cysteine as an auxiliary reagent. (hindawi.com)
  • Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
  • Intensiometric genetically encoded biosensors, based on allosteric modulation of the fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of neural activities and other cellular biochemical events. (nature.com)
  • C57BL/KaLwRij mice were implanted subcutaneously with unilateral murine green fluorescent protein-expressing 5TGM1 tumors and divided into 3 independent groups: untreated, treated beginning week 2 after tumor implantation, and treated beginning week 3 after tumor implantation. (snmjournals.org)
  • Two weeks post-stroke, mice received intramuscular injections of pseudorabies virus (PRV-152), a trans-synaptic retrograde herpes virus driving expression of green fluorescent protein (GFP), into the affected contralesional forelimb to label neurons in descending tracts to the forelimb musculature. (frontiersin.org)
  • In this study we present a preclinical fluorescence imaging system to detect sentinel lymph nodes labeled with this fluorescent tracer. (spie.org)
  • A photon-number resolving detector or a Hanbury Brown and Twiss-type arrangement of two or more single-photon detectors should be used to detect fluorescent signal. (nist.gov)
  • A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)
  • These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. (vectorlabs.com)
  • The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
  • The magnetic Fe 3 O 4 @SiO 2 nanoparticles (NPs) functionalized with 8-chloroacetylaminoquinoline as a fluorescent sensor for detection and removal of Zn 2+ have been synthesized. (hindawi.com)
  • The fabricated electrodes were applied for the detection of dopamine and trace metals as well as to generate electrochemically a Prussian Blue modified sensor. (scielo.br)
  • An actuator, the reverse of a sensor , is a device that converts an electrical signal to an action. (genomicglossaries.com)
  • Chapter 2 describes a project to develop a molecular sensor with a Noxide-bipyridine complex as a binding site and for signaling the association. (utexas.edu)
  • A fluorescent dye (FD)-based miniaturized sensor has previously been developed: a zigzag microchannel which mixes two streams under 30 s. (lu.se)
  • A viral inactivation and two polishing steps were reproduced, sending a sample of the product pool after each phase directly to the microfluidic sensor for aggregate detection. (lu.se)
  • An additional UV sensor was connected after the micromixer and an increase in its signal would indicate that aggregates were present in the sample. (lu.se)
  • 14 ] investigated selective and sensitive "turn-on" fluorescent Zn 2+ sensors based on di- and tripyrrins with readily modulated emission wavelengths. (hindawi.com)
  • In addition, due to more advantages of silica, such as nontoxicity, biocompatibility chemical inertness, no swelling, and transparency, it is a possible option to develop the nontoxic, biocompatible, and recoverable Zn 2+ -selective fluorescent sensors on Fe 3 O 4 @SiO 2 NPs surface. (hindawi.com)
  • This dissertation describes work aimed at the development of fluorescent sensors for various analytes in solution. (utexas.edu)
  • Methods The limit of detection (LOD) of the Idylla EGFR prototype assay was assessed by cell line dilution studies. (bmj.com)
  • The artus HBV QS-RGQ Kit is used with QIAsymphony RGQ, the automated system for pathogen detection, and is not for use with manual assay setup. (qiagen.com)
  • Transcreener Rac1 Assay technology uses a simple but highly effective method that consists of an antibody selective to GDP and a far-red fluorescent tracer. (bellbrooklabs.com)
  • artus HBV Kits are based on the amplification and simultaneous detection of a specific region of the HBV genome using real-time PCR. (qiagen.com)
  • amplification: See signal amplification, target amplification ( and gene amplification ). (genomicglossaries.com)
  • Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. (vectorlabs.com)
  • The BDProbeTec CT Chlamydia trachomatis Amplified DNA Assays are based on the simultaneous amplification and detection of target DNA, using amplification primers and a fluorescent labeled detector probe. (cdc.gov)
  • The Amplification Microwells are sealed to prevent contamination and then incubated in a thermally controlled fluorescent reader which monitors each reaction for the generation of amplified products. (cdc.gov)
  • Greenwald, E. C., Mehta, S. & Zhang, J. Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks. (nature.com)
  • Our extensive validation and advanced technical support ensures that all of our IHC-P-validated antibodies can be used for fluorescent multiplex IHC. (cellsignal.com)
  • Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
  • And its ability for in-vivo multi-channel fluorescence detection at subcellular level is verified. (jpier.org)
  • Yonghong Shao, "Multi-Laser Scanning Confocal Fluorescent Endoscopy Scheme for Subcellular Imaging (Invited)," Progress In Electromagnetics Research , Vol. 169, 17-23, 2020. (jpier.org)
  • The Zeiss 780 confocal laser-scanning inverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells. (lu.se)
  • dual-labeled hybridizable probe for detection and quantification of amplified DNA junction sequence. (nxtbook.com)
  • The immunosensor was successfully applied in the detection and quantification of PB1-F2 in infected mouse lungs and cell lines, providing temporal expression profiles of PB1-F2 during viral infection. (omicsonline.org)
  • Indeed, the swelling and erosion kinetics ( Supplementary Fig. S1a ), gelation time ( Supplementary Fig. S1b ) and adhesion to biological tissues ( Supplementary Fig. S1c ) of PEG:dextran were unaffected by 5% fluorescent PEG (demonstrated using the following formulation of dextran, D10-50-14, and PEG, P8-10-40, as described in Supplementary Table S2 ). (nature.com)
  • During a normal operation, a flow cytometer collects signal from each biological entity for only a short time (lO's of microseconds to a millisecond). (nist.gov)
  • This is useful in biological assays or security detection technologies. (chemistryviews.org)
  • The Imaging Core Facility offers microscopy systems that enable advanced imaging of fluorescent and non-fluorescent live cells and fixed-stained biological specimens. (lu.se)
  • The detection limit of the analytical HBV PCR is not reduced. (qiagen.com)
  • Here, topics such as molecular interactions in solution, single-analyte sensing using preorganized scaffolds, installation of signaling groups for detection of binding, analytical techniques for complexation and sensing using multiple component sensing ensembles are reviewed. (utexas.edu)
  • A detection system that records and interprets the hybridization signal. (wikipedia.org)
  • The covalent nature of tyramide-tyrosine engagement allows for heat-mediated removal (stripping) of primary/secondary antibody pairs bound to the antigen, while preserving the antigen-associated fluorescence signal. (cellsignal.com)
  • Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
  • The Detection mix included 5 μg/mL GDP antibody and 2 nM GDP Alexa 633 tracer. (bellbrooklabs.com)
  • RADT test performance could be enhanced using different antibody labeling and signal detection techniques. (bvsalud.org)
  • GDP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout. (bellbrooklabs.com)
  • Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)
  • Furthermore, this composite presented reliable detection of the streptavidin biomolecule in the gold surface with good signal-to-noise ratio (SNR). (scielo.br)
  • It has been a challenge to achieve the adequate performance needed for the reliable detection of single copy losses or gains of very small regions. (bmj.com)
  • These procedures allow the detection of cellular fluorescent signals throughout the brain in an unbiased manner. (frontiersin.org)
  • When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. (uni-muenchen.de)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. (vectorlabs.com)
  • AMCA Streptavidin can be used on frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies. (vectorlabs.com)
  • Enables detection of low abundance targets. (cellsignal.com)
  • A post-PCR melt curve analysis enables the detection of nonspecific PCR products, which melt at different temperatures than the specific PCR product. (cdc.gov)
  • To study this sensory selection process, we trained male and female mice in a selective detection task in which mice learn to respond to rapid stimuli in the target whisker field and ignore identical stimuli in the opposite, distractor whisker field. (jneurosci.org)
  • This model of selective attention proposes that both attended and unattended signals enter an early sensory store. (jneurosci.org)
  • We developed a non-invasive imaging technique that tracks material erosion in vivo through a fluorescent tag covalently attached to components of model materials. (nature.com)
  • Materials erosion was calculated from the decay in total material fluorescence signal using non-invasive In Vivo Imaging System (IVIS). (nature.com)
  • With its multi-laser scanning, multi-channel detection, flexible probing, and in-vivo imaging abilities it will become a powerful tool in bio-chemical research and diagnostics, such as the investigation of the transport mechanism of nano-drugs in small animals. (jpier.org)
  • The fluorescent dye-based method utilizes a dye that emits fluorescence when incorporating itself into double-stranded DNA. (cdc.gov)
  • Since 2014, the Advanced Molecular Detection (AMD) program has worked to integrate the latest next-generation sequencing technology with bioinformatics and epidemiology expertise to improve public health. (cdc.gov)
  • artus HBV PCR Kits are ready-to-use molecular detection kits for real-time PCR. (qiagen.com)
  • For target stimuli, we observed strong signal activation in primary somatosensory cortex (S1) and frontal cortices, including both the whisker region of primary motor cortex (wMC) and anterior lateral motor cortex (ALM). (jneurosci.org)
  • We trained mice in a detection task with both target and distractor stimuli. (jneurosci.org)
  • Multispectral imaging systems support software enabling linear unmixing of fluorophores (and chromogens) that have overlapping excitation/emission spectra and thus promote multiplex target detection with superior resolution. (cellsignal.com)
  • These systems rely on a reporter enzyme-which signals the presence of the target and produces the luminescence-and a substrate, usually added separately. (the-scientist.com)
  • Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. (uni-muenchen.de)
  • The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. (hindawi.com)
  • The addition of functionalized Fe 3 O 4 @SiO 2 NPs into the acetonitrile solution of Zn 2+ had an effect of visual color change as well as significant fluorescent enhancement. (hindawi.com)
  • and (ii) the new Finecare RADT (Ab-coated fluorescent beads). (bvsalud.org)
  • In so-called optical multiplexing, multiple optical signals are generated by an irradiated material. (chemistryviews.org)
  • Here, we are going to look at the principles behind fluorescent dye-based detection. (cdc.gov)
  • In addition to an absolute calibration, because the complete list of all photoelectronic detections is retained, the time-resolving statistical analysis identifies temporal properties of signal (nonclassical) and noise (classical). (nist.gov)
  • Nonisotopic DNA detection system employing elastase and fluorogenic rhodamine substrate. (anaspec.com)
  • First of all, the magnetic Fe 3 O 4 NPs as core in the Fe 3 O 4 @SiO 2 NPs would facilitate the magnetic separation and recuperation from the detection system within the magnetic field. (hindawi.com)
  • In this work, 8-chloroacetylaminoquinoline (CAAQ) was synthesized and covalently linked to Fe 3 O 4 @SiO 2 NPs surface to establish an efficient system for the detection and removal of Zn 2+ (labeled as Fe 3 O 4 @SiO 2 -CAAQ). (hindawi.com)
  • Absorption and fluorescent signals will be visible from phenyl rings, tryptophan, and bound cofactors such as ATP and adenine (which is fluorescent). (nanomedicine.com)
  • The colorless and nonfluorescent (Z-Ala-Ala-Ala-Ala)2Rh110 is hydrolyzed to highly fluorescent rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. (anaspec.com)
  • This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)
  • Initial direct viral detection is typically performed using an upper respiratory tract (URT) specimen. (medscape.com)
  • Schistosoma mansoni is one of the most widespread species, and its routine diag- nosis is based on microscopic detection of parasite eggs in stool samples. (cdc.gov)
  • Three fluorescent photomultiplier detecting channels of red, green and blue can record multi-color fluorescence signals from single sample site simultaneously. (jpier.org)
  • We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. (rsc.org)
  • Detection using TACS-Sapphire, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. (rndsystems.com)
  • The Detection Mix was added with the substrate to start the reaction and incubated at 30°C. Readings were taken with a CLARIOstar plate reader. (bellbrooklabs.com)
  • A 3-plex Fluorescent mIHC analysis of paraffin-embedded human breast cancer using PD-L1 (E1L3N ® ) XP ® Rabbit mAb #13684 (green), CD3ε (D7A6E ™ ) XP ® Rabbit mAb #85061 (yellow), and CD8α (C8/144B) Mouse mAb (IHC Specific) #70306 (Red). (cellsignal.com)
  • The most commonly used dye for fluorescent- dye based detection is SyBr Green. (cdc.gov)
  • Some fatty acids (e.g., arachidonic acid) are important in cell signaling. (thermofisher.com)
  • With our setup, at a given concentration of fluorophores (per cell), we accumulate the optical signal from many exposures (many cells) and statistically find second-order correlation functions and their dependence on various time delays. (nist.gov)
  • This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
  • Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
  • 1 IU/ml corresponds to 8.21 copies/ml for detection of HBV DNA on the Rotor-Gene Q. The conversion factor is an approximation based on an average factor across the assay's dynamic range. (qiagen.com)
  • A positive signal indicates an intact left IRT-rep gene junction has been detected and amplified, representing the maximum possible rcAAV contamination level present in the rAAV vector sample being analyzed. (nxtbook.com)