Fluorescent labeling reagents. Medical search. Frequent questions

When paired with fluorescent labeling (e.g., fluorescent protein, immunofluorescence, chemical dyes) and high content confocal microscopy, Corning 3D Clear reagent allows for complete 3D cell culture model characterization and more accurate drug screening. (dksh.com)
More specifically, this invention relates to asymmetric benzoxanthene dyes useful as fluorescent labeling reagents. (justia.com)
First, it is difficult to find a collection of dyes whose emission spectra are spectrally resolved, since the typical emission band half-width for organic fluorescent dyes is about 40-80 nanometers (nm) and the width of the available spectrum is limited by the excitation light source. (justia.com)
Second, even if dyes with non-overlapping emission spectra are found, the set may still not be suitable if the respective fluorescent efficiencies are too low. (justia.com)
Third, when several fluorescent dyes are used concurrently, simultaneous excitation becomes difficult because the absorption bands of the dyes are widely separated. (justia.com)
We are pleased to offer a broad range of premium fluorescent dyes that span the whole visible and near infrared spectrum, for incorporation into your custom peptides. (anaspec.com)
NHS Ester of Fluorescent Dyes enable one-step labeling & detection of both proteins (e.g. antibodies) via targeting of lysine and any other primary amine-containing macromolecules. (stratech.co.uk)
(Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively. (stratech.co.uk)
Alkyne-containing Fluorescent dyes can be used for the fluorescent labeling of Azide-tagged molecules via a Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC). (stratech.co.uk)
Fluorescent PEG - PEGylation reagents labeled with fluorescent dyes, fluorescein FITC, rhodamine, or near infrared (NIR) dyes. (creativepegworks.com)
Label-free detection, on the other hand, is proving to be a highly sensitive method of measurement for endogenous targets in live cell assays, and eliminates the need for tags, dyes or specialized reagents or engineered cells-reducing the resources required for assay development, simplifying assay design, minimizing liabilities created by the use of labels and enabling the use of native cells for greater biological relevance. (drugdiscoverynews.com)
Green Fluorescent Protein, GFP) and staining with fluorescently conjugated antibodies or fluorescent dyes. (sigmaaldrich.com)
With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel ® flow cytometry antibodies and dyes. (sigmaaldrich.com)
Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
Microscopy requires high contrast for the best image which is provided by fluorescence and fluorescent dyes. (3dbiology.com)
These dyes, or labels, attach to proteins when you incubate your tissue in their solution for a period of time. (3dbiology.com)
Other words for it used commonly are stains, fluorescent dyes, and labeling reagents. (3dbiology.com)

FITC surface labelled fluorescent particles are used as calibration particles for flow cytometry. (proscitech.com.au)
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. (mobitec.com)
5-FITC is more widely used than 6-FITC isomer for protein labeling. (mobitec.com)

The method will enable multiplex identification of antibody labels and the project will develop new antibodies suitable for high sensitivity assays. (europa.eu)
The BDProbeTec CT Chlamydia trachomatis Amplified DNA Assays are based on the simultaneous amplification and detection of target DNA, using amplification primers and a fluorescent labeled detector probe. (cdc.gov)
Protein labeling reagents are used for various assays that are coupled with antigen-antibody reaction. (dojindo.com)
Overall, a conjugate prepared by NH 2 labeling is adequate for general assays while a conjugate prepared by SH labeling can be used for high-sensitivity assays. (dojindo.com)
A peptide can be labeled with a single or multiple dye(s) at the peptide N-terminus, C-terminus, or internally, for applications in protein interaction studies , to trace the presence of biomolecules in cells, enzyme activity assays , etc. (anaspec.com)
Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers. (selectscience.net)
As advances continue to be made in screening technologies, the use of labels in traditional drug discovery screening assays has been criticized as producing undesirable and unanticipated interactions that can compromise screening data and lead to false conclusions. (drugdiscoverynews.com)
In December 2009, we reported that Caliper Life Sciences entered into an agreement with SRU Biosystems to use its label-free BIND technology to offer new functional assays as part of its discovery alliances and services. (drugdiscoverynews.com)
In April 2010, we reported that ForteBio chose Tecan as its preferred automation partner for real-time, label-free assays to support bioprocess and drug discovery workflows. (drugdiscoverynews.com)
In November 2010, we brought you the news that ACEA Biosciences Inc. and Vivo Biosciences Inc. agreed to work together to develop label-free and real-time cell-based assays for the xCELLigence System, which is co-developed by Roche and ACEA and marketed by Roche Applied Science. (drugdiscoverynews.com)
In small-molecule lead characterization, label-free assays are playing a pivotal role in validating the direct binding of small molecule and fragments to target protein. (drugdiscoverynews.com)
Also, labeled assays show end-point results only and do not allow real-time kinetic measurements such on-rates, off-rates and affinity measurements, which lead to critical information being missed, or weak binding compounds being missed in a screen. (drugdiscoverynews.com)
Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)

TThe project aims to develop the two-photon excitation (TPX) method for the measurement of fluorescence from antibody labels on the surface of micro- and nano-sized carrier particles, The proposed two-photon excitation (TPX) method which is based on fluorescence caused in micro-volumes by two-photon excitation combineshe most advanced technology in micro-fluidistics, micro-mechanics, laser scanning microscopy and flow cytometry applications. (europa.eu)
Our Tyramide Amplification Kits have been demonstrated to be robust and versatile for multi-color fluorescence imaging, compatible with dye-labeled antibodies and various cell staining methods (see Figure 1). (biotium.com)
This is effective in checking the reaction speed of each reagent and in analyzing time-course changes in color development and fluorescence intensities. (hamamatsu.com)
1) After fluorescent dye tracing, immunofluorescent staining (IF), or fluorescence in situ hybridization (FISH), mount brain sections upon adhesive microscope slides, such as gelatin-coated slides. (bioenno.com)
The labeled cells can be visualized using fluorescent imaging, quantified using flow cytometry, and isolated using fluorescence-activated cell sorting (FACS).The ALDEFLUOR™ fluorescent reagent system offers a novel approach to the non-immunological identification, evaluation and isolation of neural stem and progenitor cells. (stemcell.com)
The AF488 NT labeling mix contains specially optimized dUTP-XX-AF488 for incorporation into DNA by nick translation using DNA Polymerase I. The excellent stability and quantum yield of the fluorophore combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications. (jenabioscience.com)
Independent of light scatter analysis, fluorescence measurements are achieved by transfection and expression of common fluorescent proteins (e.g. (sigmaaldrich.com)
The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence. (cdc.gov)

The group also developed a reagent to detect anti-H5 avian influenza virus antibody, a fluorescein-labeled protein that binds only with the antibody. (medicaldesignbriefs.com)
Label IT® CX-Rhodamine Labeling Kit Label IT® Fluorescein Labeling Kit Label IT® TM-Rhodamine Labeling Kit *Each Full Size Kit contains sufficient reagents to Label 100 µg of nucleic acid. (pdfmedarticles.com)
The Label IT Nucleic Acid Labeling Reagents covalently attach CX-rhodamine, fluorescein, digoxin, biotin, Cy™3, Cy™5, dinitrophenol (DNP), and TM-rhodamine labels to nucleic acid bases within DNA or RNA without impacting hybridization performance, and therefore can be used in almost any molecular biology application (excitation and emission wavelengths for each fluorescent Label IT Reagent are listed in Table 1). (pdfmedarticles.com)

The enzyme labeling kits can be used for labeling small molecules, such as organic chemicals, amino acids, peptides, or oligonucleotides. (dojindo.com)
For peptides labeled with a dye and quencher, see our FRET peptides page. (anaspec.com)
We deliver fluorescent dye-labeled peptides in research grade or as critical raw material. (anaspec.com)

The overall protocols for Kit-NH 2 and Kit-SH for labeling macromolecules and small molecules g are indicated in Figs. 3 and 4. (dojindo.com)
Liquid crystal molecules are capable of controlling the polarization direction of fluorescent light, while the microfluidic device has a number of microchannels as a measurement vessel. (medicaldesignbriefs.com)
The reagent was made by reproducing hemagglutinin (HA) protein fragments, which are expressed on the surface of H5 avian influenza virus, through gene recombination and by labeling fluorescent molecules to the fragments. (medicaldesignbriefs.com)
the lysine side chain (with a -NH2 terminus, a primary amine) is commonly used to covalently attach labels to antibody molecules. (novusbio.com)
Mirus' Label IT Nucleic Acid Labeling Technology represents a new class of labeling reagents designed to efficiently and reproducibly attach marker molecules to nucleic acids (DNA or RNA) in a simple one-step reaction within minutes. (pdfmedarticles.com)

Of these, fluorescent, biotin, and enzyme labeling reagents are used most frequently for antibody labeling. (dojindo.com)
Nick translation labeling is based on the reverse activities of Polymerase I and DNase I. DNase I is able to introduce randomly distributed nicks to double stranded DNA at low enzyme concentrations. (jenabioscience.com)
The well balanced polymerase / nuclease activities of the enzyme mix ensure the generation of highly labeled double stranded DNA fragments. (jenabioscience.com)
Traditional nonradioactive Labeling methods (random priming, nick translation) are enzyme mediated and thus inherently difficult to control. (pdfmedarticles.com)
This Labeled-nucleotide is not the preferred substrate for the enzyme and may compromise the efficiency of the reaction and introduce a Labeling bias. (pdfmedarticles.com)

In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. (neb.com)
The Amplification Microwells are sealed to prevent contamination and then incubated in a thermally controlled fluorescent reader, which monitors each reaction for the generation of amplified products. (cdc.gov)
Dojindo's labeling kits contain centrifugal Filtration Tubes that are used for protein purification, conjugation reaction, condensation, and buffer exchange. (dojindo.com)
Labeling requires 1) purification of protein and buffer exchange, 2) labeling reaction, and 3) purification and recovery of the conjugate. (dojindo.com)
The tyramide reaction does not interfere with the binding of dye-labeled antibodies or other fluorescent staining reagents. (biotium.com)
This mode measures the reaction process of one reagent and follows its changes over time. (hamamatsu.com)
The kit is good for three conjugation reactions with each reaction labeling up to 5 mg IgG. (mobitec.com)
The simplicity of the Labeling reaction, and the fact that only a single reagent is required, suggests that this technology can be widely used in a variety of nucleic acid Labeling applications. (pdfmedarticles.com)
In contrast, the Label IT Labeling reactions are nondestructive, easy to control, and can be scaled up or down by either the size of the reaction or the desired Labeling density. (pdfmedarticles.com)
If there is a need to increase or decrease the density of Labels in the final product, simply modify the ratio of Labeling reagent to nucleic acid during the Labeling reaction or adjust the incubation time of the labeling reaction. (pdfmedarticles.com)
2. Prepare the Labeling reaction according to the example shown below. (pdfmedarticles.com)
Antibodies are typically labeled through direct conjugation (either as commercially available products or conjugation kits allowing the end user to perform the reaction) or through the use of secondary antibodies. (sigmaaldrich.com)
DBD-d(and l)-beta-proline, new fluorescent chiral derivatization reagents, were synthesized from the reaction of 4-(N,N-dimethylaminosulfonyl)-7- fl uoro-2,1,3-benzoxadiazole (DBD-F) with beta-proline. (unboundmedicine.com)

Proteins are labeled endogenously with antibody epitope tags or fluorescent proteins to visualize them with unprecedented specificity and ease. (wraltechwire.com)
By reproducing fragments of spike proteins expressed in the novel coronavirus, and using them as the reagent, the analyzer should be able to detect anti-coronavirus antibodies. (medicaldesignbriefs.com)
Common molecular biology applications include analysis of recombinantly expressed fluorescent proteins that have induced expression in various cell types to elucidate gene function or for in vivo cell tracking. (sigmaaldrich.com)

Most protein labeling reagents have similar reactive sites, such as succinimidyl ester (NHS) for amino groups and maleimide or bromoacetamide for sulfhydryl groups. (dojindo.com)
Dojindo offers the following protein labeling reagents and kits for protein research and proteomics. (dojindo.com)
Sometimes, protein labeling with NH 2 -reactive compounds is not favorable because of the potential blockage of the protein's active site. (dojindo.com)
Therefore, labeling at the NH 2 group on a protein is fairly reliable and simple method for preparing a conjugate. (dojindo.com)
The sugar chain of a protein is also a possible labeling site with hydrazide compounds. (dojindo.com)
Dojindo offers a wide variety of protein labeling kits. (dojindo.com)
Additionally, the kit's unique buffer system prevents aggregation of protein and conjugate during labeling and storage of the conjugate. (dojindo.com)
The reagent uses a synapsin promoter to drive neuronal expression of the red fluorescent protein mKate2 and minimize crossover to non-neuronal cells. (genengnews.com)
Next, using genetically modified plants, which express fluorescent protein and are responsive to the level of auxin, we demonstrated the possibility to deliver encapsulated agrochemicals into cells. (nature.com)
The Odyssey 28 KD Loading Indicator - 700 nm is a single 28 kDa recombinant protein directly labeled with a near-infrared fluorescent dye. (licor.com)
Our fluorescent fusion protein is changing the phenotype. (cellculturedish.com)

Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel ® Flow Cytometry Reagent Preparation protocol. (sigmaaldrich.com)
Performing flow cytometry analysis involves using various flow cytometry antibodies and labels. (sigmaaldrich.com)

To convert to an aldehyde group before labeling with a hydrazide labeling. (dojindo.com)

From AnaSpec: Truncated fluorescently labeled amyloid-beta peptide containing metal binding residues to study its metal interaction characteristics and kinetics. (anaspec.com)

NGS relies on sequencing by synthesis: the sequence of a template DNA strand is determined by synthesizing a complementary strand from fluorescently labeled bases. (acs.org)
The NPs were fluorescently labeled and loaded with agrochemicals such as the plant hormone auxin. (nature.com)

The reagents segment will control most of the peptide synthesis market. (fairfieldmarketresearch.com)
and the market availability of various peptide synthesis reagents can all be attributed to the large share of this segment. (fairfieldmarketresearch.com)

The processed sample is added to the Priming Microwell, which contains the amplification primers, fluorescent labeled detector probe, and other reagents necessary for amplification. (cdc.gov)
Arsenic-based Nrf2 activators, fluorescent biarsenical labeling reagent (FlAsH) and phenylarsine oxide (PAO), were used to probe binding of arsenic to Keap1. (cdc.gov)
AF488 Nick Translation Labeling Kit contains all reagents (except template and materials for purification of the probe) required for nick translation labeling providing a highly efficient, easy-to-perform and rapid labeling technology. (jenabioscience.com)

Fluorogenic substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released). (anaspec.com)

A fluorophore, sometimes called a fluorochrome, is simply a fluorescent compound that upon light excitation emits light. (3dbiology.com)

The TPX plate reader will allow multiplexed analysis through the use of fluorescent labelling agents which have different emission wavelengths. (europa.eu)

The most common reactive site used is the NH 2 group from lysine residue because of the simple process of labeling an NH 2 terminal under physiological conditions. (dojindo.com)
However, activity loss due to blockage of active sites by labeling is rare in the case of antibody labeling with NH 2 -reactive reagent. (dojindo.com)
The second most common labeling method is the use of SH-reactive compounds. (dojindo.com)
Then, SH-reactive compounds such as maleimide and bromoacetyl compounds are applied in labeling the protein's SH groups. (dojindo.com)
There are just four common approaches to labeling, which differ in the creation of the reactive label. (novusbio.com)

After a period of incubation (one hour), excess secondary reagent is washed away and the amount of label associated with the primary antibody is quantified. (novusbio.com)
With direct detection, the prior covalent attachment of the label to the primary antibody means that only a single incubation step with the antigen is required and only a single round of wash steps, as opposed to two rounds of incubation and wash steps with indirect detection. (novusbio.com)
During incubation, the primary antibody dissociates from the antigen with the secondary reagent and in subsequent wash steps, resulting in amplification due to a diminishing amount of primary antibody. (novusbio.com)

Some techniques commonly used in the labeling of antibodies and other biomolecules are outlined in the following section. (novusbio.com)

Undoubtedly, the main reason for this is that direct labeling of primary antibodies is relatively complicated and, historically, antibody labeling has been carried out only by those with specialist knowledge of chemical-modification techniques. (novusbio.com)

The efficiency of DNA labeling can be estimated by calculating the ratio of incorporated fluorophores to the number of bases in the fragment (dye / base). (jenabioscience.com)

HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. (selectscience.net)
Following treatment of the plants with the different fluorescent-labeled NPs, fluorescent microscopic techniques enabled to localize the NPs and observe the accumulation in the plant's vascular system. (nature.com)

This invention relates generally to fluorescent dye compounds useful as molecular probes. (justia.com)
Molecular movements of the reagent bound with the antibody will be smaller in the liquid, producing a different degree of polarization from the reagent not bound with the antibody. (medicaldesignbriefs.com)

Store all other supplied reagents, including the G50 microspin purification columns, at 4°C. Do not use the G50 microspin columns if they have been stored at -20°C or colder. (pdfmedarticles.com)

The fluorescent reagent, NeuroLight Red™, specifically labels both primary neurons and human iPSC derived neurons when grown in co-culture with astrocytes, allowing for quantitation using the IncuCyte NeuroTrack™ software. (genengnews.com)
NeuroLight Red is a lentiviral-based reagent that has been designed to efficiently transduce multiple types of neurons with low toxicity. (genengnews.com)
The fluorescent images of neurons provided sufficient signal amplitude over background to enable the masking of neurites (bottom left, yellow) and cell body clusters (bottom right, blue) using NeuroTrack software and user-defined processing definitions. (genengnews.com)
In addition to rat primary forebrain neurons, NeuroLight Red can label other primary neuronal subtypes (hippocampal, cortical, and dorsal root ganglion) as well as iPSC-dervied neurons (data not shown). (genengnews.com)
Labeling neurons with a flueorescent reagent such as NeuroLight Red provides a means to develop a medium-throughput assay to study neurite dynamics in a co-culture setting. (genengnews.com)
Figure 2 shows data from a 96-well assay measuring neurite length over twelve days using NeuroLight Red labeled rat forebrain neurons with rat cortical astrocytes (NeuroPrime™ Cell Kit). (genengnews.com)
D) AAV1 transfected neurons were labeled with anti-RFP and Alexa Fluor™ 647 antibodies. (bioenno.com)

If a suitable reagent is developed, this technology could be used to detect antibodies against SARS-CoV-2, the causative virus of COVID-19. (medicaldesignbriefs.com)
Our analyzer could be used to conduct other bio tests if suitable reagents are developed," says Tokeshi. (medicaldesignbriefs.com)

There are two types of kits available: NH 2 labeling (Kit-NH 2 ) and SH labeling (Kit-SH). (dojindo.com)
Note: AnaTag kits are not intended for peptide labeling. (mobitec.com)

The microplate to be used will significantly reduce reagent requirements and dispense with the multiple washing currently required in conventional methods. (europa.eu)
Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods. (justia.com)
In the case of automated DNA sequencing, multicolor labeling allows for the analysis of all four bases in a single lane thereby increasing throughput over single-color methods and eliminating uncertainties associated with inter-lane electrophoretic mobility variations. (justia.com)
With traditional labeling methods, a basic understanding of the principles of chemical modification is required because the antibody and/or label must be chemically 'activated' before the labeled antibody (or 'conjugate') can be formed. (novusbio.com)
This guide is a user friendly tool that allows you to learn the basics of common antibody labeling methods. (novusbio.com)
A 'label' is also incorporated into the assay using one of two methods (see below - Direct vs. Indirect Detection Methods) to provide measurability. (novusbio.com)
There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. (genomicglossaries.com)
Methods include the pre-column method, in which the analytes are labeled in advance, and the post-column method, in which the analytes are labeled with derivatizing reagents after HPLC separation. (fujifilm.com)

The supplied standard Labeling protocol will yield Labeling efficiencies of approximately one Label every 20-60 base pairs of double-stranded DNA. (pdfmedarticles.com)

The Strand Displacement Amplification (SDA) reagents are dried in two separate disposable microwell strips. (cdc.gov)
What about the amplification afforded by the secondary reagent in indirect detection? (novusbio.com)

Fluorochrome-labeled antibodies distinguish populations and subpopulations of WBCs. (cdc.gov)

Now, companies are introducing sequencing platforms that separate fluorescent labeling from the extension of the complementary DNA strand, touting improvements in accuracy that result from optimizing each step. (acs.org)

Each Trial Size Kit contains sufficient reagents to Label 25 µg of nucleic acid. (pdfmedarticles.com)
In addition, these types of reactions generate Labeled products that are not representative of the starting nucleic acid but rather consist of a series of Labeled samples over a variable size range. (pdfmedarticles.com)
The Labeling efficiency of these reactions is dictated by the enzyme's ability to incorporate a 'Labeled-nucleotide' precursor into a growing nucleic acid chain. (pdfmedarticles.com)
Use only purified nucleic acid (A260/A280 between 1.8 and 2.2) in the Labeling reactions. (pdfmedarticles.com)

High research and development expenses by biotechnology companies, an increase in the number of biotechnology firms, an increase in biopharmaceuticals, and technological advancements are projected to drive the growth of the reagents segment during the forecast period. (fairfieldmarketresearch.com)
The rising use of biotechnology reagents in medicines, commercial applications, and fundamental research are just a few of the factors expected to drive the growth of the reagents sector. (fairfieldmarketresearch.com)

1. Prior to each use, warm the vial containing the Label IT Reagent to room temperature and quick spin to collect the pellet. (pdfmedarticles.com)

However, a conjugate prepared by SH labeling provides a better signal than that prepared by NH 2 labeling because of site-specific labeling. (dojindo.com)

Progress reports containing any problems encountered during shipping or receipt of specimens, summary statistics for each control pool, QC graphs, instrument calibration, reagents, and any special considerations are submitted to NCHS quarterly. (cdc.gov)

Corning now offers 3D Clear Tissue Clearing reagent that can be used in a tissue clearing technique, designed specifically to support imaging for 3D cell culture models and plate-based high throughput processing. (dksh.com)
The use of Corning 3D Clear Tissue Clearing reagent allows for a significant increase in dose response sensitivity (left), as the technique allows for the entire population of cells within a 3D cell culture model to be characterized. (dksh.com)
And most recently, last month, we reported that Activiomics and Belgian biopharma UCB will apply Activiomics' Targeted In-depth Quantification of cell Signaling (TIQUAS) phospho-proteomics platform-a system that is quantitative, label-free and applicable to cell and tissue samples-in a collaborative effort to elucidate signaling mechanisms of therapeutic antibodies in relevant cell-based systems. (drugdiscoverynews.com)

3. For imaging only dead cells, you can use NucGreen® Dead 488 ReadyProbes® Reagent SKU R37109 for green ( https://www.lifetechnologies.com/order/catalog/product/R37109 ) or SKU R37113 for red ( https://www.lifetechnologies.com/order/catalog/product/R37113 ). (cellculturedish.com)

Critics also argue that labeled technologies typically require the use of genetically modified cell lines that can alter cellular behavior. (drugdiscoverynews.com)

In order to achieve high sensitivity and selectivity of the analyte in high performance liquid chromatography (HPLC), labeling is performed with a derivatizing reagent appropriate for the detection method. (fujifilm.com)

In presence of dye-labeled dUTP Polymerase I incorporates labeled dUTP instead of dTTP. (jenabioscience.com)
After each base is incorporated by a polymerase and imaged, its fluorescent tag is removed and another base can be added. (acs.org)

By eliminating the need for radioactive labels, safety is enhanced and the environmental impact of reagent disposal is greatly reduced, resulting in decreased costs for analysis. (justia.com)

Thermo Fisher Scientific is committed to providing instruments, reagents, and solutions for plant and animal genotyping applications: simple, scalable, and affordable next-generation solutions that will help drive remarkable agricultural innovations. (thermofisher.com)
What applications do label-free technologies make possible that are either impossible with current technologies, or so impractical as to have made them unapproachable without label-free technology? (drugdiscoverynews.com)
All applications in biotherapeutic discovery and development such as epitope mapping and binning and antibody screening are impossible, or at least highly cumbersome, with labeled techniques. (drugdiscoverynews.com)
Mirus has found that this Labeling density is sufficient to allow sensitive detection for the majority of applications. (pdfmedarticles.com)

This allows accurate evaluation of lot-to-lot differences in reagent performance and measurement variations over time. (hamamatsu.com)
To make the measurement, serum collected from birds was mixed with the reagent and left for 15 minutes. (medicaldesignbriefs.com)

Significant attention will be paid also to reducing waste and reagent use, The technology to be developed will enable the multinational SME:s involved to created significant products for global markets to be commercialised by the partners through an agreed action plan, taking into consideration the IP-rights developed. (europa.eu)

Dye labeled secondary antibodies can be co-incubated with the HRP-conjugated secondary or HRP-streptavidin from the tyramide kit. (biotium.com)
Alternatively, using indirect detection, the label is covalently attached to a secondary antibody, which is allowed to bind to the primary antibody during the immunoassay. (novusbio.com)
Excess unbound primary antibody is then washed away and a labeled secondary reagent is added. (novusbio.com)

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