Fluorescent gene silencing. Medical search. Frequent questions

Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. (researchsquare.com)
Meanwhile, FZ supported systemic expression of fluorescent proteins mNeonGreen and mCherry in N. benthamiana and soybean. (researchsquare.com)
This overview considers these technologies and how they have been used to identify novel viral and plant proteins or genes involved in disease and resistance responses, as well as defense signaling. (springer.com)
These approaches include analysis of spatial and temporal responses by plants to infection, and techniques that allow the expression of viral genes transiently or transgenically in planta, the expression of plant and foreign genes from virus vectors, the silencing of plants genes, imaging of live, infected cells, and the detection of interactions between viral proteins and plant gene products, both in planta and in various in vitro or in vivo systems. (springer.com)
Our research performance involves developing novel and innovative drug delivery technologies that can carry proteins, RNAs, or gene therapies to the target tissues safely and efficiently. (varbi.com)
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation (1). (gbiosciences.com)
Fluorophores for STORM and SMLM include synthetic dyes, fluorescent proteins (FPs), and even Quantum Dots (QDots). (microscopyu.com)
Recently, we elucidated the developmental role of tobacco ( Nicotiana tabacum L.) NtTTG2 in association with 12 genes that putatively encode AUXIN RESPONSIVE FACTOR (ARF) proteins, including NtARF8, NtARF17, and NtARF19. (biomedcentral.com)

They mediate epigenetic silencing of gene expression in a wide range of developmental and homeostatic processes in many tissues, including the brain. (escholarship.org)
Methylation at the 5 position of the cytosine base, when followed by guanine (CpG) in the promoter region of a protein-coding gene, is an epigenetic modification that has been shown to be involved in gene silencing. (mit.edu)

Por meio da clonagem de regiões de genes ortólogos já utilizados como alvo de silencimento em outras espécies de insetos (V-ATPase A, Receptor de Ecdisona e Arginina Kinase), e de genes com função específica identificadas após a caracterização do transcritoma de D. saccharalis (Juvenile Hormone Epoxide Hydrolase, Neverland e Quitina Sintase) foram conduzidos ensaios de RNAi. (usp.br)
Os genes V-ATPase A, receptor de Ecdisona, Arginina Kinase, Juvenile Hormone Epoxide Hydrolase e Quitina Sintase apresentaram redução significativa no número de transcritos em larvas, demonstrando a viabilidade do uso de RNAi em D. saccharalisneonatas. (usp.br)
Gene silencing by RNA interference (RNAi) rapidly became a widely investigated approach, adopted in various aspects of biology. (usp.br)
In the present work, target genes with the potential to affect the normal development of D. saccharaliswere searched, together with an efficient dsRNA delivery approach to test the target-genes to validate the use of the RNAi in D. saccharalis. (usp.br)
Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, similar to miRNA, and operating within the RNA interference (RNAi) pathway. (wikipedia.org)
In 1998, Andrew Fire at Carnegie Institution for Science in Washington DC and Craig Mello at University of Massachusetts in Worcester discovered the RNAi mechanism while working on the gene expression in the nematode, Caenorhabditis elegans. (wikipedia.org)
The ability to utilize the RNA interference (RNAi) machinery for silencing target-gene expression has created a lot of excitement in the research community. (onacademic.com)
In the present study, we used a cytomegalovirus (CMV) promoter-driven DNA template approach to induce short hairpin RNA (shRNA) triggered RNAi to block exogenous Enhanced Green Fluorescent Protein (EGFP) and endogenous No Tail (NTL) gene expressions. (onacademic.com)
From RNA interference (RNAi) to CRISPR, there are several methods that researchers can use to manipulate gene function, each with its own strengths and weaknesses. (technologynetworks.com)
RNA interference (RNAi) was the primary tool for disrupting gene expression (along with zinc finger nucleases (ZFNs) and transcription activator-like effector nuclease (TALENs)) but newer CRISPR-based technologies, including CRISPR knockout (CRISPRko) and CRISPR interference (CRISPRi) are viable options. (technologynetworks.com)
As gene-modulating tools, RNAi and CRISPR technologies are often grouped together, despite their disparate modes of action and considerations for effective usage. (technologynetworks.com)
In contrast to RNAi, CRISPR-based gene manipulation requires a guide RNA and a programmable Cas endonuclease protein. (technologynetworks.com)
Achieving a mechanistic and experimental middle ground between CRISPR-mediated gene knockout, whereby gene expression is reduced at the DNA level, and RNAi, in which target knockdown is not permanent, is CRISPRi. (technologynetworks.com)

Subsequently, we will evaluate the efficacy of long-term silencing by shRNA MC delivery by RVG-exosomes to silence EGFP expression in the brain of transgenic EGFP-mice. (neurodegenerationresearch.eu)
We have elucidated the developmental function of NtTTG2 by analyses of NtTTG2 -overexpressing ( TTG2 + ) and NtTTG2 silencing ( TTG2 i) transgenic tobacco lines compared to wild-type (WT) plants or a transgenic control line [ 4 ]. (biomedcentral.com)
Quantification of Linoleylethanolamide dsRed+ cells from zebrafish embryos Transgenic embryos expressing green fluorescent protein (GFP) under the control of the promoter and red fluorescent protein (dsRed) under the promoter 16 were dissociated into single cells and analyzed by flow cytometry.14 O-dianisidine staining and heme content For O-dianisidine staining ten embryos/condition were collected and stained according to standard procedure. (bioerc-iend.org)

It interferes with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, preventing translation. (wikipedia.org)
Thus silencing the gene that encodes that mRNA. (wikipedia.org)
miRNAs typically silence genes by repression of translation and have broader specificity of action, while siRNAs typically work with higher specificity by cleaving the mRNA before translation, with 100% complementarity. (wikipedia.org)
However, the mere presence of small RNAs (Supplementary Table 1 ) or terminally modified mRNA fragments 9 does not result in indefinite RNA silencing. (nature.com)
The shGFP-generating plasmid significantly inhibited the EGFP expression viewed under fluorescent microscope and reduced by 70.05 +/- 1.26% of exogenous EGFP gene mRNA levels compared with controls by Q-PCR. (onacademic.com)
When the RNA fragments complex with a complementary (target) mRNA sequence and an endogenous silencing complex, mRNA is fragmented, preventing its translation. (technologynetworks.com)

CD47 silencing will be assessed by flow cytometry and immunohistochemistry analysis together with antibodies for staining of specific cell types. (varbi.com)

Gene knockdown by transfection of exogenous siRNA is often unsatisfactory because the effect is only transient, especially in rapidly dividing cells. (wikipedia.org)
The experiment of knockdown endogenous MMP1 gene was performed in MeWo cells. (kinasepathway.com)
Knockdown of these genes increased the number of chromocenters within the rod nuclei and increased euchromatin area distributed throughout the cell body as compared to the control groups. (arvojournals.org)
Confirmation that the observed phenotype can be attributed to the knockdown of these genes was achieved by analyzing multiple retinas injected with the original shRNA. (arvojournals.org)
Additional validation was achieved by injecting and imaging a second knockdown construct of each gene and by performing rescue experiments. (arvojournals.org)
A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA RID A-5315-2009 [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA ,2003:1844---1848. (onacademic.com)
Finally, effects of FUS on HIV gene transcription are also exhibited genome wide, where FUS mainly occupies gene promoters at transcription starting sites, while its knockdown leads to an increase in AFF4 and Cdk9 occupancy on gene promoters of FUS affected genes. (biomedcentral.com)
Methods employed to knockdown or knockout gene products can answer specific questions about gene function, in addition to helping identify and develop therapeutics. (technologynetworks.com)
From this cleavage and repair process, sequence insertions or deletions (indels) often cause errors in translation, leading to functional gene, and subsequent protein, knockdown. (technologynetworks.com)
Therefore, we hypothesized that Pitx2 is a candidate gene for inhibiting pluripotency gene expression and that Pitx2 knockdown might enhance reprogramming in fibroblast cells. (usc.edu)

Transfection efficiency was determined by calculating the ratio of fluorescent to total cells after transfection with a plasmid containing the green fluorescent protein (GFP) gene. (screenfect.com)
The aim of the present investigation is to study the effect of MMP-2 gene silencing by transfection of MMP-2 shRNA expression plasmids (pMMP-2on radiation-induced DNA damage in human normal dermal fibroblasts (HDFs) and MCF-7 cells. (biomedcentral.com)

Chromatin Insulator Elements Block Transgene Silencing in Engineered hESC Lines at a Defined Chromosome 13 Locus. (ca.gov)
However, when these cells were differentiated into specific lineages, reduction in reporter expression was observed, indicating transgene silencing. (ca.gov)
Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)
Here we introduce mating as a simple approach to reproducibly initiate RNA silencing of a single-copy transgene that can last for hundreds of generations. (nature.com)
Transgene expression was analysed in Arabidopsis T‐DNA transformants carrying defined numbers and arrangement of different reporter genes. (fu-berlin.de)
High, stable transgene expression was observed in plants containing two copies of the β‐glucuronidase (GUS) gene, two or four copies of the green fluorescent protein (GFP) gene and two, four or six copies of the streptomycin phosphotransferase (SPT) gene. (fu-berlin.de)
Thus, the mere presence of multiple promoter and/or transgene sequences did not result in gene silencing. (fu-berlin.de)

siRNAs and their role in post-transcriptional gene silencing(PTGS) was discovered in plants by David Baulcombe's group at the Sainsbury Laboratory in Norwich, England and reported in Science in 1999. (wikipedia.org)
Instead, post‐transcriptional gene silencing (PTGS) correlated with the copy number of the highly expressed transgenes. (fu-berlin.de)

An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. (researchsquare.com)
Independent silencing of the NtARF8 gene more strongly repressed tobacco growth than silencing the NtARF17 or NtARF19 gene and more effectively eradicated the growth enhancement effect of NtTTG2 overexpression. (biomedcentral.com)
NtARF8 also contributed to NtTTG2-regulated seed production as concurrent NtTTG2 and NtARF8 overexpression played a synergistic role in seed production quantity, whereas concurrent silencing of both genes caused more severe seed abortion than single gene silencing. (biomedcentral.com)

The green fluorescent protein (GFP) gene was driven by EF1alpha or CMV early enhancer/chicken beta actin (CAG) promoter. (ca.gov)
These data proved the feasibility of the CMV promoter-driven shRNA expression technique to be used to inhibit exogenous and endogenous gene expressions in zebrafish in vivo. (onacademic.com)
Suppression of zebrafish VEGF gene by cytomegalovirus promoter-driven short hairpin constructs induces vascular development defects and down regulation NRP1 expression [J]. BIOLOGIA ,2009:1025---1031. (onacademic.com)
Concordantly, depletion of FUS elevates the occupancy of AFF4 and Cdk9 on the viral promoter and activates HIV gene transcription. (biomedcentral.com)
Additionally, DRP1 is a downstream target gene of FENDRR, and FENDRR formed an RNA-DNA triplex with the promoter of DRP1, which led to an increase in DRP1 promoter methylation that decreased the transcriptional level of DRP1. (biomedcentral.com)
in addition, they indicated recurrent mutations in the promoter region of telomerase (TERT) that reactivates the gene. (medscape.com)

However, fruit flies with a mutation in the gene that encodes the enzyme xanthine dehydrogenase develop kidney stones that are remarkably like their human counterparts, rich in calcium hydroxyapatite. (newswise.com)
16. Knorr, D.A. and Dawson, W.O. (1988) A point mutation in the tobacco mosaic capsid protein gene induces hypersensitivity in Nicotiana sylvestris . (springer.com)
At the time of diagnosis, the vast majority of these tumors will exhibit a mutation of the IDH1/2 gene and up to one half will exhibit gene mutation or deregulation of the expression of the TP53 gene. (medscape.com)
As the disease progresses and converts into leukemia, further gene mutation occurs, and a proliferation of leukemic cells overwhelms the healthy marrow. (medscape.com)

The shRNA targeting endogenous NTL gene resulted in obvious NTL phenotype of 30 +/- 4% and decreased the level of their corresponding mRNAs up to 54.52 +/- 2.05% compared with nontargeting control shRNA. (onacademic.com)

The ingestion of double-stranded RNA (dsRNA) molecules with identity to regions of essential genes of the insect-pest can result in the target gene knock-down and, consequently, to deficient phenotypes. (usp.br)
Cells with GFP expression indicated it was successful in construction of target gene reporter plasmid. (kinasepathway.com)

Researchers in the laboratory of Mark E. Davis at the California Institute of Technology have been using small interfering RNA (siRNA), sometimes known as silencing RNA, to "silence" specific genes that are implicated in certain malignancies. (news-medical.net)

In particular, described here is the first normal hESC fluorescent reporter line that robustly expresses GFP in both the undifferentiated state and throughout dopaminergic lineage differentiation. (ca.gov)
Selected genes from the target list were knocked down through in vivo electroporation in native retinas using shRNA-mediated gene silencing with a rod-specific fluorescent reporter in postnatal day 0 mice. (arvojournals.org)
In none of the cases analysed were tandem repeat arrangements of transgenes and/or inverted repeat (IR) T‐DNA structures sufficient to trigger silencing of the different reporter genes. (fu-berlin.de)

A ingestão de moléculas de RNA dupla fita (dsRNA) com identidade a regiões de genes essenciais de insetos-praga pode resultar no silenciamento destes genes, levando a fenótipos deficientes. (usp.br)
Neste contexto, o presente trabalho teve como objetivo buscar genes alvos para o silenciamento com potencial para impedir o desenvolvimento normal da D. saccharalis e estabelecer uma forma de entrega do dsRNA eficiente para o teste de genes, visando assim validar o uso da técnica para a espécie. (usp.br)
O silenciamento dos genes alvo requer quantidades elevadas de dsRNA, superiores aos obtidos por transcrição in vitro, o que limita a viabilidade de ensaios com maiores replicatas e para determinar efeitos biológico. (usp.br)
The mechanism by which natural siRNA causes gene silencing through repression of translation occurs as follows: Long dsRNA (which can come from hairpin, complementary RNAs, and RNA-dependent RNA polymerases) is cleaved by an endo-ribonuclease called Dicer. (wikipedia.org)
This toxicity can be avoided by switching to short interfering RNAs (siRNAs), which are truncated RNA duplexes that bypass the aforementioned dsRNA cleavage step, retain targeted gene silencing abilities of dsRNA, and do not trigger the deleterious immune response in mammalian cells. (technologynetworks.com)

FZ-based silencing constructs induced robust silencing of phytoene desaturase genes in N. benthamiana , multiple soybean accessions, and cowpea. (researchsquare.com)

Scientists from the University of Twente, the Netherlands, have successfully exploited the optical properties of fluorescent nanoparticles to broaden the scope of single-cell microscopy. (news-medical.net)
Firstly, fluorescent ASOs conjugated to a range of ligands will be studied for cellular uptake in different cell lines, including cardiomyocytes, using flow cytometry and confocal microscopy. (varbi.com)
B) Examples of fluorescent microscopy images of HEK293T cells transfected with different lipids from the library showing GFP positive cells. (screenfect.com)

Cell viability assay was performed right Selleckchem Inhibitor Library after the fluorescent analysis. (kinasepathway.com)

Through this extensive and ongoing screen, it has been shown that some target genes affect chromatin organization in rod photoreceptor cells, moving away from the inverted pattern. (arvojournals.org)

Killing the messenger: Short RNAs that silence gene expression [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY ,2003:457---467. (onacademic.com)

The second aim is to develop this system for long-term silencing within the brain using minicircles expressing shRNA (shRNA MC). (neurodegenerationresearch.eu)
Finally, we will silence the expression of human WT and phospho-mimic S129D alpha-synuclein expressed in mice using shRNA MC. (neurodegenerationresearch.eu)
This study evaluated the effect of matrix metalloproteinases-2 (MMP-2) gene silencing using MMP-2 shRNA expression plasmids (pMMP-2) on IR induced cytotoxicity and DNA damage by MTT, dead green, γH2AX and comet assays in human normal dermal fibroblasts (HDFs) and MCF-7 human breast cancer cells. (biomedcentral.com)

In 2001, the expression of a specific gene was successfully silenced by introducing chemically synthesized siRNA into mammalian cells (Tuschl et al. (wikipedia.org)
To explore novel mechanisms and gene targets for HTN, the gene expression profiles of renal biopsy samples obtained from 2 healthy living donor controls and 5 HTN patients were determined by single-cell RNA sequencing. (bvsalud.org)
Key hub genes expression were validated by the Nephroseq v5 platform. (bvsalud.org)
Together, our data identify a distinct cell-specific gene expression profile, pathogenic inflammatory signaling and potential cell-cell communications between endothelial cells or mesangial cells with other renal resident cells in HTN. (bvsalud.org)
RNA sequencing analysis indicated that the N protein changed the expression of certain genes involved in immune-related functions and four important signaling pathways, including JAK-STAT, TNF, NF-κB and MAPK signaling pathways, which suggested that the N protein may not only regulate the expression of genes involved in the process of resisting viral infection in macrophages of the immune system, but also change cellular signal processing. (bvsalud.org)
14. Gowda, S., Wu, F.C., Scholthof, H.B., and Shepherd, R.J. (1989) Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the fulllength RNA transcript. (springer.com)
A candidate list of 720 genes was established to have differential expression between rod and cone photoreceptors, as determined by RNA-sequencing transcriptional profiling data. (arvojournals.org)
These data reveal another layer of gene expression control at the post-transcriptional level. (nature.com)
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. (nature.com)
The ability to rapidly change gene expression patterns is essential for differentiation, development, and functioning of the brain. (escholarship.org)
Throughout development, or in response to environmental stimuli, gene expression patterns are tightly regulated by the dynamic interplay between transcription activators and repressors. (escholarship.org)
In this study, we used fluorescent immunohistochemistry to map their expression patterns throughout the adult mouse brain. (escholarship.org)
This study investigated fluorescent protein expression in the shallow-water coral Acropora yongei during a 30 day laboratory photoacclimation experiment in the context of its dinoflagellate symbionts. (biologists.com)
Changes that stably alter gene expression across generations presumably require particular conditions that are currently unknown. (nature.com)
Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. (nature.com)
The first aim is to evaluate the efficacy of normal and modified siRNA delivery by RVG-exosomes to silence alpha-synuclein expression in mice. (neurodegenerationresearch.eu)
Successfully transfected cells can be visualized due to the expression of the green fluorescent protein (GFP) and cytotoxicity is directly proportional to the number of brighly stained nuclei in the upper panels. (screenfect.com)
Gene expression and silencing in polyploids. (tsukuba.ac.jp)
Gene expression and epigentic alterations as consequences of genome duplication. (tsukuba.ac.jp)
A widely used approach for studying gene function is to reduce or disrupt its basal expression. (technologynetworks.com)
Both platforms require RNA-based reagents, and can lower gene expression, but they differ in more ways than they are similar. (technologynetworks.com)
Extremely lethal consequences of IR causes double-strand breaks, which are mainly responsible for genomic instability, altered gene expression, and cell death. (biomedcentral.com)
Expression profiling during ES cell differentiation suggests that the gene, Pitx2, is highly expressed in differentiating cells, which are no longer in an ES cell-like state. (usc.edu)

Lead conjugates will then be tested for functional silencing of target genes in different organs in mice following systemic administration of ASOs. (varbi.com)

As a model system we will target the fluorescent EGFP protein. (neurodegenerationresearch.eu)

Results were evaluated by phenotype observation and real-time fluorescent quantitative reverse-transcription polymerase chain reaction (Q-PCR). (onacademic.com)
As transcription silencing is key for establishing the HIV reservoir, significant efforts have been made to understand the mechanism that regulate HIV gene transcription, and the role of the elongation machinery in promoting this step. (biomedcentral.com)
FUS inhibits the activation of HIV transcription by AFF4 and ELL2, and silences overall HIV gene transcription. (biomedcentral.com)
Our observations show that FUS regulates both HIV and global gene transcription and modulates viral latency, thus can potentially serve as a target for future therapy that sets to reactivate HIV from its latent state. (biomedcentral.com)
Early studies on the regulation of gene transcription of the proviral human immunodeficiency virus (HIV) have laid the foundations to our current understanding of how metazoan transcription elongation is regulated [ 1 ]. (biomedcentral.com)

Cells with an apparent absence of green fluorescence indicated gene silencing. (kinasepathway.com)

Physical mapping of ribosomal genes by Fluorescent Molecular in situ Hybridization. (tsukuba.ac.jp)

This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans . (nature.com)

Nuclear receptor corepressor 1 (NCoR1) and silencing mediator for retinoid or thyroid-hormone receptors (SMRT) are the best characterized transcriptional co-repressors from a molecular point of view. (escholarship.org)

The upregulated genes in tubules of HTN were mainly participating in inflammatory signatures including IFN-Î³ signature, NF-κB signaling, IL-12 signaling and Wnt signaling pathway. (bvsalud.org)
Molecular data have indicated a direct progression from diffuse astrocytoma to anaplastic astrocytoma to glioblastoma in patients whose tumors bear mutations in the IDH1/2 genes and TP53 genes, a finding that marks the pathogenic pathway of 5% of all glioblastomas. (medscape.com)

The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
Virus-induced gene silencing (VIGS) is an alternative functional genomics tool for soybean because it does not involve the generation of inheritable changes. (researchsquare.com)

Subsequently, dose-and time response studies will be performed using qPCR to determine the magnitude of gene silencing with the different conjugates across different cell types. (varbi.com)
Alpha-synuclein silencing will be evaluated by qPCR, western blot and immnuhistochemistry throughout the brain. (neurodegenerationresearch.eu)

These features are shared by other natural antisense transcripts and can confer regulatory activity to an artificial antisense to green fluorescent protein. (nature.com)
Green fluorescent protein (GFP) concentration measured by Western blotting changed reversibly with light intensity. (biologists.com)
Using yeast surface display, I engineered an MBD variant with improved binding affinity, concatenated it, and expressed it in E. coli as a green fluorescent protein (GFP) fusion. (mit.edu)

Similar findings were made in bone marrow cells from gene-targeted mice lacking Bim and/or Puma infected with FLT3-ITD and treated with inhibitor, where loss of Puma only provided transient protection from apoptosis, but loss of Bim preserved clonal survival upon FLT3-ITD inhibition. (ashpublications.org)
Within your postdoctoral studies you will study a range of novel ASO-conjugates for their ability to silence genes in cell culture and in mice models, with focus on heart delivery. (varbi.com)

Since in principle any gene can be knocked down by a synthetic siRNA with a complementary sequence, siRNAs are an important tool for validating gene function and drug targeting in the post-genomic era. (wikipedia.org)
We have developed a gene therapy vehicle based on targeted exosomes which we have shown are capable of selectively targeting the delivery of siRNA to brain resulting in gene silencing after intravenous administration. (neurodegenerationresearch.eu)

RNA silencing: a conserved antiviral immunity of plants and animals [J]. VIRUS RESEARCH ,2004:109---115. (onacademic.com)

Proximal tubules in HTN highly expressed hub genes including BBOX1, TPM1, TMSB10, SDC4, and NUP58, which might be potential novel targets for proximal tubular injury. (bvsalud.org)

The HTN endothelium upregulated cellular adhesion genes (ICAM2 and CEACAM1), inflammatory genes (ETS2 and IFI6) and apoptosis related genes (CNN3). (bvsalud.org)

However, only silencing of Bim, or its direct transcriptional activator FOXO3a, abrogated apoptosis efficiently. (ashpublications.org)

This study investigates genes that may play a role in regulating these nuclear architecture patterns in photoreceptor cells. (arvojournals.org)
To determine the magnitude of silencing in individual cells, different methods will be employed. (varbi.com)
Malat-1 silencing will be analyzed using single cell RNA sequencing of cells that have taken up Cy3-labelled ASO conjugates in vivo. (varbi.com)
In addition, MMP-2 gene silencing using pMMP-2 decreased comet tail length in irradiated HDFs but increased in irradiated MCF-7 cells. (biomedcentral.com)
Preliminary reprogramming was assessed by the detection of red fluorescent in cells infected with red fluorescent protein. (usc.edu)
Using alkaline phosphatase staining to mark ES cells, we identified ES-like colonies that were reprogrammed from fibroblast cells in the plate where OSKM factors were present and the Pitx2 gene was silenced. (usc.edu)
This study demonstrated that it is possible to reprogram human dermal fibroblast cells to a pluripotency state although further studies are needed using different reprogramming methods and other pluripotency genes. (usc.edu)
A thorough understanding of the Pitx2 gene will lead to more efficient reprogramming strategies, enabling large-scale production of iPS cells and producing an alternative to embryonic stem cells for use in research and therapy for diabetes, heart failure, Parkinson's disease and other neurodegenerative diseases. (usc.edu)

This, combined with the relatively small numbers of seed each soybean plant produces, makes it extremely challenging to generate mutant seed libraries that cover even 50% of genes. (researchsquare.com)
In contrast, plant growth was not affected by silencing additional nine NtTTG2 -regulated NtARF genes. (biomedcentral.com)

It may, additionally, also include a transfer of gene segment from another organism into the host cell. (biomedcentral.com)
Cell culture and gene silencing The human lymphoblast K562 cell line (ATTC number CCL-243? (bioerc-iend.org)

In flies genetically predisposed to develop stones, researchers can track the origins of the condition, and can also screen drugs, medical therapies, and other genes that counter stone formation. (newswise.com)

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research. (sigmaaldrich.com)

Genes related to zinc transport in particular seemed to play a major role, demonstrating the importance of the element in stone formation. (newswise.com)

Here we identify a nuclear-enriched lncRNA antisense to mouse ubiquitin carboxy-terminal hydrolase L1 ( Uchl1 ), a gene involved in brain function and neurodegenerative diseases 11 . (nature.com)
Another growing area of research in the Andaloussi lab pertains to the delivery of antisense oligonucleotides (ASOs) that are used to either silence genes or induce splice switching. (varbi.com)

The chromosomal abnormalities commonly involve the MLL gene (11q23). (medscape.com)

this is what enables the molecules to form the RNA-Induced Silencing Complex (RISC). (wikipedia.org)

Proteins8

Epigenetic2

RNAi13

Transgenic3

MRNA6

Assessed by flow cytometry1

Knockdown10

Transfection2

Transgene7

PTGS2

Overexpression4

Promoter6

Mutation4

Endogenous1

Target gene2

Specific genes1

Reporter3

DsRNA5

Constructs1

Microscopy3

Assay1

Chromatin1

RNAs1

ShRNA3

Expression23

Systemic1

EGFP1

Transcription5

Fluorescence1

Situ1

Sequences1

Mediator1

Pathway2

Functional2

QPCR2

Green3

Mice2

SiRNA2

Immunity1

Highly expressed1

Cellular1

Transcriptional1

Cells8

Plant2

Cell2

Therapies1

Widely1

Zinc1

Antisense2

Chromosomal1

Complex1