• For viable cells, only the cell-surface amines are available to react with the dyes while for the necrotic cells or the other cells with compromised membranes, the reactive dyes react with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. (creativebiomart.net)
  • The fluorescent dyes provided in the kits are retained in cells by reacting with cellular components. (creativebiomart.net)
  • Among the reactive dyes, amine-reactive dyes are most often used to prepare various bioconjugates for immunochemistry, histochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor binding and other biological applications since amino groups are either abundant or easily introduced into biomolecules. (biomol.com)
  • Amine-reactive dyes are most often used to prepare bioconjugates for immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling and fluorescent analog cytochemistry. (biomol.com)
  • However, current monitoring methods fall short, said the study's first author Evan W. Miller, a post-doctoral researcher in the lab of Roger Tsien, PhD, Howard Hughes Medical Institute investigator, UC San Diego professor of pharmacology, chemistry and biochemistry and 2008 Nobel Prize co-winner in chemistry for his work on green fluorescent protein. (ucsd.edu)
  • Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio without precipitation during conjugation. (fishersci.ca)
  • Chromeo Py-Dyes have been shown to work efficiently and with high sensitivity in different methods of protein electrophoresis. (activemotif.com)
  • The labeled protein is ready to use immediately, and any possible background from unbound dye is eliminated. (activemotif.com)
  • Because of their unique features, Chromeo Py-Dyes (Chromeo P429, P503, P540 and P543) have been used successfully in a number of "no-wash" applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. (activemotif.com)
  • Amine-reactive pyrylium dyes have been used as a pre-stain in protein electrophoresis. (activemotif.com)
  • The mutant form GGBP/H152C of the D-glucose/D-galactose-binding protein with the solvatochromic dye BADAN linked to cysteine residue Cys 152 can be used as a potential base for a sensitive element of glucose biosensor system. (peerj.com)
  • Green fluorescent protein - EGFP redirects here. (enacademic.com)
  • Yellow fluorescent protein - (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. (enacademic.com)
  • Fluorescent fusions of the N protein of phage Mu label DNA damage in living cells. (viictr.org)
  • Dyes for analysis of protein aggregation WO 2011/065980 A2, 03.06.2011. (org.ua)
  • Use of squaraine dyes to visualize protein during separations // Patent Application Publication WO 2008/027821 A1, 06.03.2008. (org.ua)
  • These fluorophores bind via a vinyl sulfone functional unit to amines and thiols under slightly basic condition rendering an antibody, protein marker, or other moeity fluorescent to aid in microscope visualization. (akinainc.com)
  • When the protein unfolds, the exposed hydrophobic surfaces bind the dye, resulting in an increase in fluorescence by excluding water. (wikipedia.org)
  • Representative photoresistant fluorescent dyes such as Alexa Fluor® 488 and ATTO 488 are also known to encounter photodegradation in STED microscopy, making it difficult to conduct continuous live-imaging of biological systems while retaining high resolution. (innovations-report.com)
  • The Fungi Fluor ® Pneumocystis kit offers a fast, fluorescent staining procedure for Pneumocystis carinii in bronchial specimens. (polysciences.com)
  • ASHBURN, Va., Oct. 19, 2017 - To better illuminate the inner workings of cells, researchers have developed a way to adjust the properties of fluorescent dyes deliberately, resulting in an expanded palette of dyes that are bolder, brighter and more cell-permeable. (photonics.com)
  • With the extensive selection available, Atto dyes can replace commonly used fluorescent dyes. (sigmaaldrich.com)
  • The commonly used fluorescent dye YOYO-1 (YOYO) has, using bulk techniques, been demonstrated to stain DNA heterogeneously at substoichiometric concentrations. (chalmers.se)
  • The water-soluble dye is composed of a central perylene chromophore, a polyphenylene dendrimer scaffold, and a polymer shell bearing multiple amine groups. (acs.org)
  • The internal location of the microelectrode has been confirmed by electrophoretically microinjecting the water-soluble dye Lucifer yellow CH into giant mitochondria or mitoplasts. (sciencemag.org)
  • From simple organelle specific dyes for imaging cell structure and determining cell viability, to more complex dyes and reporter assays for monitoring cell signaling, death pathways, and toxicity, every product is developed and reliably manufactured to provide sensitivity, specificity, and convenience. (enzolifesciences.com)
  • Multi-color organelle-specific dyes for co-localization studies and monitoring changes in cell structure. (enzolifesciences.com)
  • In order to address the demand for better performing dyes for two-photon based imaging, we have prepared a new series of reactive fluorophores tailored for multiphoton imaging. (spie.org)
  • The Invitrogen™ eBioscience™ Super Bright polymer dyes represent a suite of bright fluorophores excited by the violet laser (405 nm). (selectscience.net)
  • The SureColor F9470H also offers businesses the option of printing with two genuine fluorescent inks - Yellow and Pink - which brings bright, vivid colors to the production of sportswear, workwear and fashion items. (digitaloutput.net)
  • Typically, the dyes were dissolved in a hydrophilic organic solvent and then suddenly placed in an aqueous environment, where they spontaneously produce molecular assemblies. (coursehero.com)
  • The wide house-stock collection containing about 2,000 dyes of various classes (polymethine cyanines, styryles, coumarines, and metallocomplexes) is used as a basis for performing of such studies. (org.ua)