• A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. (labome.org)
  • Serum specimens from hospitalized patients with pneumonia were tested for antibodies to Streptococcus pneumoniae pneumolysin, pneumolysin immune complexes, C polysaccharide, surface protein A, Haemophilus influenzae, and Branhamella catarrhalis by Dr. M. Leinonen, National Public Health Institute, Oulu, Finland, as reported previously ( 11 - 13 ). (cdc.gov)
  • Acute- and convalescent-phase serum specimens from 150 patients also had been previously tested by enzyme-linked immunosorbent assay (ELISA) for antibodies to L. pneumophila serogroups 1-6 by Yu ( 14 ). (cdc.gov)
  • Through the use of a filter-paper technique to collect daily serum samples, it was possible to determine that the initial increase in FA response was on the 7th to 9th day of patency. (ajtmh.org)
  • This technique requires each Ag to be tested separately and involves long incubation periods, making ELISA labor-intensive and time-consuming as well as requiring significant amounts of Ag and serum or plasma. (asm.org)
  • We prepared primary anti- Rickettsia akari and anti- Rickettsia slovaca rabbit antibodies (in serum) in our experimental animal facility at Biomedical Research Center. (biomedcentral.com)
  • Diagnosis may be made by culture of the fastidious Gram-negative bacillus from lung tissue or pleural fluid, demonstration of the organism in lung tissue by the direct fluorescent antibody technique, or documentation of a significant rise in serum titer by indirect fluorescence. (annals.org)
  • Indirect fluorescent antibody (IFA) tests ( Figure 20.29 ) are used to look for antibodies in patient serum. (openstax.org)
  • Patient serum is spread over the smear and anti-treponemal antibodies, if present, are allowed to bind. (openstax.org)
  • The serum is washed off and a secondary antibody added. (openstax.org)
  • On examination, the T. pallidum bacteria will only be visible if they have been bound by the antibodies from the patient's serum. (openstax.org)
  • BACKGROUND AND PURPOSE: The aim of this study was to describe clinical and paraclinical characteristics of all Danish patients who tested positive for anti-voltage-gated potassium channels (VGKC)-complex, anti-leucine-rich glioma-inactivated 1 (LGI1) and anti-contactin-associated protein-2 antibodies in the serum/cerebrospinal fluid between 2009 and 2013 with follow-up interviews in 2015 and 2016. (sdu.dk)
  • Histological examination of the cutaneous lesions, indirect immunofluorescence on rat bladder epithelium, and western blot of human keratinocyte proteins identified anti-epidermal antibodies in the patient's serum. (bvsalud.org)
  • In the field serum samples tested, the seroprevalences of anti-N. caninum antibodies were 3.2%, 4.6%, and 6.4% in the NAT, IFAT and ELISA, respectively. (bvsalud.org)
  • Patient serum diffuses from one well through the gel and reacts with a known specific antigen (or antibody) which diffuses through the gel from a second well. (specialtylabs.com)
  • Magnitude of antibody level in patient' serum can be assessed by indirect immunofluorescence and enzyme linked immunosorbent assay. (elsevier.com)
  • This study sought to adapt the new SAT multiplex system for measuring antibodies against nine malarial vaccine candidate antigens, including recombinant proteins from two variants of merozoite surface protein 1, two variants of apical merozoite antigen 1, erythrocyte binding antigen 175, merozoite surface protein 3, and peptides from the circumsporozoite protein, ring erythrocyte surface antigen, and liver-stage antigen 1. (asm.org)
  • Electrophoresis (EP) is a technique for separation of ionic molecules (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. (specialtylabs.com)
  • Immunoelectrophoresis (IEP) is a two-step procedure which first involves the electrophoretic separation of proteins, followed by the linear diffusion of antibodies into the electrophoretic gel from a trough which extends through the length of the gel adjacent to the electrophoretic path. (specialtylabs.com)
  • The clinicopathologic diagnosis of paraneoplastic pemphigus was confirmed by indirect immunofluorescence staining of murine bladder epithelium, and the presence of IgG autoantibodies against envoplakin, periplakin, and 170 kDa protein. (elsevier.com)
  • In five out of seven patients, the culture supernatants tested by indirect immunofluorescence on frozen sections of normal human skin and rabbit lip showed the production of autoantibodies with different specificities: basal epidermal cells, whole epidermis, Merkel cells, fibroblasts endothelial cells, etc. (elsevier.com)
  • A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). (int-res.com)
  • Ito T, Olesen NJ, Skall HF, Sano M, Kurita J, Nakajima K, Iida T (2010) Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. (int-res.com)
  • FACS analysis using indirect immunofluorescence techniques indicated that approximately 84% of the synaptosomal fraction was labeled by monoclonal antibody (mAb) A2B5 and thus appeared to be of neuronal origin. (elsevier.com)
  • Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilized, even when the size of the cell is identical to other subset species. (specialtylabs.com)
  • It has been more than 50 years since antinuclear antibodies were first discovered and found to be associated with connective tissue diseases. (scribd.com)
  • Connective tissue diseases (CTD) are a group of autoimmune disorders which are characterized by presence of antinuclear antibodies (ANA) in the blood of patients. (scribd.com)
  • Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti -infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
  • The antitail antibody blocks transport before the delivery of cargo to the Golgi compartment containing α-1,2 mannosidase I. Semiintact CHO clone 15B cells were incubated at 32°C in a complete transport cocktail for indicated time in the absence (closed circles) or presence (open circles) of antitail antibody. (nih.gov)
  • 1994). The cells were pelleted, resuspended in a transport cocktail that contained either 5 mM EGTA (a), 0.1 μM Ca2+ (b), or 0.1 μM Ca2+ and 10 μg of antitail antibody (c), and incubated at 32°C for 90 min. (nih.gov)
  • As shown in Fig. 4 (closed circles), semiintact CHO 15B cells incubated in the absence of antibody at 32°C processed VSV-G to the Man5 form. (nih.gov)
  • Herein we optimized this serological technique, testing four fixative solutions, for the sensitive detection of rickettsial antigens, and preservation of intracellular structures of the host cells, particularly filamentous actin. (biomedcentral.com)
  • Fluorescent cells appear after a relatively brief incubation of cells from adult-suppressed animals, whereas no fluorescent cells are detected when cells from neonatally treated animals are incubated briefly. (sciencemag.org)
  • In contrast, antireceptor antibody causes reversible blockade of responsive cells in adult-suppressed animals. (sciencemag.org)
  • Antibodies against protein kinase C (PKC), parvalbumin, and calbindin were used to label rod bipolar cells, AII amacrine cells, and cone bipolar cells, respectively. (lu.se)
  • The AB5 antibody was used to label ganglion cells. (lu.se)
  • Cloyd, MW & Bigner, DD 1977, ' Contained indirect viable cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses ', Journal of Clinical Microbiology , vol. 5, no. 1, pp. 86-90. (utmb.edu)
  • The reorientation of the microtubule organizing center during cell migration into a wound in the monolayer was directly observed in living wound-edge cells expressing gamma-tubulin tagged with green fluorescent protein. (mblwhoilibrary.org)
  • Analysis of HBV-transfected cells by indirect immunofluorescence or immunoelectron microscopy revealed the expression of viral antigens and cytoplasmic viral particles, respectively. (unab.cl)
  • To localize the step in transport sensitive to antibody, we made use of the CHO clone 15B cell line, which lacks the cis/medial-Golgi enzyme N-acetylglucosamine-transferase I (GlcNAC Tr I) (Tabas and Kornfeld, 1979). (nih.gov)
  • abstract = "We describe a case of cerebellar ataxia associated with anti-Hu antibodies and benign ganglioneuroma. (elsevier.com)