• alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • Conventional techniques employed to detect NPs in tissues such as transmission electron microscopy coupled with energy dispersive x-ray spectroscopy offer superior nanoscale resolution, however pose limitations due to the high cost of sample processing and limited sample analysis throughput. (cdc.gov)
  • Confocal and fluorescence microscopy are also common techniques used to detect fluorescent NPs, however their detection ability is often obscured by tissue autofluorescence and are limited to detecting fluorescent NPs. (cdc.gov)
  • I show pictures using each technique, discuss some of their pros and cons and describe how DIC microscopy works. (canadiannaturephotographer.com)
  • For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. (gentaur.com)
  • For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen-antibody reaction to locate antigenic substances in tissues or cells. (medicilon.com)
  • This technique of labeling antibodies with fluorescent substances for antigen localization is called fluorescent antibody technique ( fluorescent antibody technique ). (medicilon.com)
  • in most applications the substance in question serves as antigen, both in antibody production and in measurement of antibody by the test substance. (theodora.com)
  • Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye ( FLUORESCENT ANTIBODY TECHNIQUE, DIRECT ) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody ( FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT ). (nih.gov)
  • Molecular and immunocytological techniques were used to study the expression of embryonic zeta (ζ), epsilon (ε), and fetal gamma (γ) globin genes in newborn cord blood, peripheral blood from men, pregnant and non-pregnant women, and in vitro mononuclear cell cultures. (hku.hk)
  • Fluorescence immunoassay technology is one of the earliest developed labeling immunoassay techniques. (medicilon.com)
  • Fluorescence immunotechnology generally uses fluorescent substances for labeling. (medicilon.com)
  • Fluorescent molecules do not convert all the absorbed light energy into fluorescence, and they are always released in more or less other forms. (medicilon.com)
  • Fluorescence efficiency refers to the percentage of fluorescent molecules that convert the absorbed light energy into fluorescence, which is proportional to the value of the emitted fluorescent light quantum. (medicilon.com)
  • Each fluorescent molecule has its specific absorption spectrum and emission spectrum (fluorescence spectrum), that is, there is a maximum absorption peak and maximum emission peak at a specific wavelength. (medicilon.com)
  • When the wavelength of the excitation light is selected to be close to the maximum absorption peak wavelength of the fluorescent molecule, and the measured light wave is close to the peak of the maximum emission light, the obtained fluorescence intensity is also maximum. (medicilon.com)
  • FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. (gentaur.com)
  • A fluorescent-antibody technique for the detection of enterotoxin-producing cells of Clostridium perfringens type A / Manuel J. Torres-Anjel, Hans P. Riemann, and Che C. Tsai. (who.int)
  • The antibodies do not exhibit non-specific binding to dissimilar NPs such as gold NPs or carbon nanotube s as demonstrated through custom-designed in vitro assays. (cdc.gov)
  • However, studies show varying results on NP skin penetration depending upon the NP size and surface chemistry, skin model used and the NP detection techniques employed. (cdc.gov)
  • The scFv antibodies are engineered with a FLAG tag to allow for secondary detection using standard immunohistochemistry methods. (cdc.gov)
  • The novel antibodies isolated when used in conjunction with other existing techniques for NP detection will comprise a powerful tool kit, and enable researchers to use them to detect NPs both in the environment and in a biological milieu. (cdc.gov)
  • A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of effect of HC virus on Newcastle disease virus in swine tissue cultures. (microbiologyresearch.org)
  • Alternative technologies using fluorescent and antibody detection have also been developed, but are not highly sensitive. (auburn.edu)
  • Daphnnia), ciliates, and some bacteria exhibit good contrast by this technique. (canadiannaturephotographer.com)
  • Application of the fluorescent-antibody technique to an ecological study of bacteria in soil. (microbiologyresearch.org)
  • Processed using the fluorescent antibody (FA) staining technique, this photomicrograph revealed the presence of a number of Streptococcus pneumoniae bacteria, that were discovered in this cerebral spinal fluid (CSF) sample. (cdc.gov)
  • Some non-fluorescent pigment substances such as methylene blue and basic fuchsin are commonly used in fluorescent antibody technology. (medicilon.com)
  • This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. (gentaur.com)
  • SYN: group i.. humoral i. i. associated with circulating antibodies, in contradistinction to cellular i.. infection i. the paradoxical immune status in which resistance to reinfection coincides with the persistence of the original infection. (theodora.com)
  • However, like all cultures, diagnosis is slow using this method, and more rapid diagnosis is possible using PCR techniques. (auburn.edu)
  • Paramushir virus was recently demonstrated to be serologically identical to Avalon virus by complement-fixation, neutralization, and fluorescent-antibody techniques (4). (cdc.gov)
  • HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique. (microbiologyresearch.org)
  • Results of search for 'su:{Fluorescent antibody technique. (who.int)
  • or it may be passive, being acquired from transfer of antibodies from another person or from an animal, either naturally, as from mother to fetus, or by intentional inoculation (artificial passive i.), and, with respect to the particular antibodies transferred, it is specific. (theodora.com)
  • In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
  • In recent years, several special fluorescent immunoassays have been developed, which are used in clinical tests like enzyme immunoassays and radioimmunoassays. (medicilon.com)
  • Additionally, the antibodies have been characterized for their binding and cross-reactivity properties to several other NPs, and some challenges associated with the isolation of the antibodies from a large library and alternative method for selection of antibodies have been discussed. (cdc.gov)
  • We have developed NP binding antibodies to commercially important NPs including QDs and TiO2 NPs using phage display technology. (cdc.gov)
  • Blood samples were collected for CT IgG antibodies testing using indirect immunofluorescence. (ufrgs.br)
  • There is a high prevalence of CT IgG antibody in Brazilian pregnant and infertile women, but we found a low prevalence of positive PCR in the urine samples. (ufrgs.br)
  • The antibodies isolated against GSH-QDs and TiO2 NPs by panning in solution, can recognize the respective NPs in skin and did not show any non-specific binding to skin samples without NPs. (cdc.gov)
  • An affinity based selection technique (biopanning) is used to identify binders from the library. (cdc.gov)
  • Immunofluorescence techniques in diagnostic microbiology / edited by Joan M. B. Edwards, C. E. D. Taylor, A. H. Tomlinson. (who.int)
  • With the recent application of new molecular diagnostic techniques, the study of vector-borne disease problems has been enhanced. (vin.com)
  • We found statistically similar prevalence of PCR and IgG antibodies between the groups. (ufrgs.br)
  • B-lymphocytes undergo isotype switching, and IgM, IgG, and IgA antibodies are found in the neuropil adjacent to the lesion. (jneurosci.org)
  • The radiation ability of fluorescent molecules will be weakened or even quenched after being irradiated by the excitation light for a long time. (medicilon.com)
  • Therefore, care should be taken to avoid direct exposure of light (especially ultraviolet light) and contact with other compounds in the storage of fluorescent materials. (medicilon.com)
  • This technique can be applied using a dark-field condenser or metal stop (e.g. coin) that fits below the condenser in the light path. (canadiannaturephotographer.com)
  • In this study, a sensitive host cell reactivation (HCR) technique was used to examine the repair capacity for DNA damaged by sunlamp exposure in fibroblast strains derived from 5 normal individuals and 8 patients representing three different diseases associated with DNA repair deficiencies. (mcmaster.ca)
  • Previous reports using this technique showed a substantial reduction in the HCR of sunlamp-exposed Ad 2 for infection of excision repair deficient fibroblasts from patients with xeroderma pigmentosum. (mcmaster.ca)
  • Ѐtude de la multiplication en cycle unique d'un clone de virus de la peste porcine classique au moyen de l'immunofluorescence. (microbiologyresearch.org)
  • This thesis discusses the discovery of novel antibodies binding QDs and TiO2 NPs and their functionality by demonstrating their binding both in vitro and in an ex vivo human skin model. (cdc.gov)
  • Therefore, a simple economical technique which can provide information on both the presence of NPs and their form in biological systems and the environment is required. (cdc.gov)