• Here we describe the use of optical window chambers in combination with photobleaching, photoactivation, and photoswitching to quantitatively measure collective tumor cell movement, proliferation, and protein dynamics in squamous cell carcinoma cells within a tumor mass in vivo . (aacrjournals.org)
  • A combination of RNA interference and Fluorescence Recovery After Photobleaching approaches demonstrated very different dynamics from one protein to the other, but also according to the stage of construction and the age of the flagellum. (mcponline.org)
  • The two other, rather new but more reliable approaches are either by detecting areas of different probe mobility on an individual image basis or by physical modeling of fluorescence loss from moving bodies. (wikipedia.org)
  • Here, using multiple quantitative fluorescence-based approaches and electron microscopy, we show that synapsin is instrumental for resisting vesicle dispersion and serves as a regulatory element for controlling lateral vesicle sharing between synapses. (nih.gov)
  • If, for example, the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will gradually recover fluorescence. (academic.ru)
  • These techniques, which are almost universally employed in both the medical and biological sciences, have spurred the development of more sophisticated microscopes and numerous fluorescence accessories. (microscopyu.com)
  • Methods- Histopathology, molecular biology, and electrophysiological and fluorescence cell death assays in slice cultures after OGD and in developing rats after intrauterine hypoxia-ischemia (HI). (ahajournals.org)
  • The points represent the fluorescence data, and the solid line is the predicted recovery curve given the first-order rate constant, k, determined by least-squares fitting of the data to the equation given in Materials and Methods. (asm.org)
  • In this study, we investigated microscopic two-dimensional and three-dimensional organization of lipid membranes comprising of PLE and their dependence on Ca 2+ concentration ([Ca 2+ ]) with fluorescence microscopy and AFM. (frontiersin.org)