Fluorescence cell movement. Medical search. Frequent questions

The newly developed fluorescence time-lapse imaging technique uses a very thin sheet of laser light to build up images into a full 3D image of the heart layer by layer. (ed.ac.uk)
Evaluates designs using sequencing, fluorescence assays, enzyme activity assays, and single cell studies using time-lapse microscopy. (washington.edu)

Cell surface distribution of lectin receptors determined by resonance energy transfer. (uconn.edu)
Cerebrospinal fluid and vesicular lesions were PCR-positive for VZV and were determined to be the vOka strain by the Centers for Disease Control and Prevention using a VZV fluorescence resonance energy transfer PCR. (cdc.gov)

Excitation light of different wavelengths may be differentially modulated to allow the fluorescence evoked by each to be demultiplexed from a single photodetector signal, for example to independently measure fluorescence evoked from the genetically encoded calcium indicator GCaMP by 470 nm and 405 nm excitation light. (nature.com)
Professor Takeshi Yasui, from the Institute of Post-LED Photonics (pLED), Tokushima University, Japan, who led the study, explains, "Our method can be interpreted as simultaneously mapping 44,400 'light stopwatches' over a 2D space to measure fluorescence lifetimes-all in a single shot and without scanning. (labmanager.com)

The base of choice for live-cell imaging, high-content applications, confocal and super-resolution. (nikon.com)

Advanced fluorescence microscopy techniques take advantage of this. (photometrics.com)
Single molecule imaging represents a subset of fluorescence microscopy techniques that uses fluorescent tags to detect and analyze individual single molecules. (photometrics.com)

They are generally more expensive, toxic, do not permeate cell membranes, and cannot be manufactured by the cell. (wikipedia.org)
Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
Verapamil affects the passage of calcium ions across cell membranes of smooth muscle cells and heart (myocardial) cells. (seagullindia.com)
Interactions of microtubule subunits and membranes followed by fluorescence techniques. (uconn.edu)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
As cell membranes are both dynamic and compositionally complex, replicating these aspects in a model membrane are essential. (lu.se)

Some proteins or small molecules in cells are naturally fluorescent, which is called intrinsic fluorescence or autofluorescence (such as NADH, tryptophan or endogenous chlorophyll, phycoerythrin or green fluorescent protein). (wikipedia.org)
The benefit that GFP and other fluorescent proteins have over organic dyes or quantum dots is that they can be expressed exogenously in cells alone or as a fusion protein, a protein that is created by ligating the fluorescent gene (e.g. (wikipedia.org)
For example, we can 'tag' signaling proteins with green fluorescent protein (GFP) and then follow their movements in the cell during hormonal stimulation in real time. (rochester.edu)
A number of photoactivatable GFP-like fluorescent proteins (PAFPs) have been reported whose fluorescence can be switched on or whose fluorescent state can be modified by relatively intense irradiation at a specific wavelength. (nih.gov)
The use of these proteins gives unique opportunities to photolabel and track fusion proteins in a living cell. (nih.gov)
To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. (ppjonline.org)
By filtering through noise, Lei was able to determine the movement of proteins in cells using fluorescent dyes, characterizing the difference in protein motion between healthy and infected cells. (oredigger.net)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
Some inverted microscopes are equipped with fluorescence imaging capabilities, which allow the user to study samples that have been labeled with fluorescent dyes or proteins. (microbiologynote.com)
Changes in vascular permeability are lial cells tethered together by junctional proteins commonly present in certain clinical diseases such as tight and adherens junctions. (bvsalud.org)

Fluorescence spectroscopy for chemical analysis reveals information on the biological samples. (horiba.com)
Since then, he has sought to apply his research techniques towards "Advanced fluorescence microscopy and linear and nonlinear spectroscopy techniques in biomedical applications. (oredigger.net)
For this purpose we employ the combination of experimental techniques in real and reciprocal spaces, including grazing incidence X-ray scattering and fluorescence, off-specular neutron scattering, flicker spectroscopy, etc. (lu.se)
Overhauser effect spectroscopy, dielectric relaxation and fluorescence spectroscopy. (lu.se)

Notwithstanding its superior sensitivity and versatility, fluorescence imaging has always been hampered by the fact that only a small percentage of emitted photons is actually detected, necessitating stronger excitation intensities, which may lead to photobleaching and photodamage ( Yang and Yuste, 2017 ). (jneurosci.org)
Time-division multiplexed illumination allows independent readout of fluorescence evoked by different excitation wavelengths from a single photoreceiver signal. (nature.com)
The Swarm delivers variable intensity blue-light optogenetic stimulation to enable membrane depolarization and red or lime-light excitation to enable fluorescence detection of the resulting changes in membrane potential or calcium levels, respectively. (frontiersin.org)
Finally, the measured signal is mathematically transformed into the frequency domain, and the fluorescence lifetime at each "pixel" is easily calculated from the relative phase delay that exists between the excitation signal at that modulation frequency versus the one measured. (labmanager.com)

They include advances in atomic force microscopy, structured illumination microscopy and other 3-D approaches, as well as imaging in single cells in animals and in plants. (cshlpress.com)
Light emitted by the indicators is transmitted back through the optic fiber, separated from the illumination light and fluorescence from other indicators using optical filters, and converted to electrical signals by high sensitivity photoreceivers or cameras. (nature.com)
Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or lasers, the evanescent field only penetrates the specimen by about 100 nm starting from the coverslip/medium interface. (leica-microsystems.com)
They may have simple illumination systems and fixed stages, and may not have advanced features such as motorized stages or fluorescence imaging capabilities. (microbiologynote.com)
They may have motorized stages, advanced illumination systems, and fluorescence imaging capabilities, as well as other specialized features such as phase contrast or DIC optics. (microbiologynote.com)
These microscopes are designed specifically for studying living cells and tissues and are equipped with features such as temperature-controlled stages, specialized illumination systems, and fluorescence imaging capabilities. (microbiologynote.com)

It also allowed quantitative imaging independent from changes in cell volume, revealing a surprisingly strong and hitherto uncovered contribution of TRPV4 channels to Na + loading on energy failure. (jneurosci.org)

We monitor intracellular calcium in the cytoplasm and the endoplasmic reticulum using several fluorescence imaging techniques. (rochester.edu)

Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)

His premature death at the age of 53 (in March 1997) cut short a remarkably creative career that included seminal contributions to understanding the physiology of smooth muscle, and the pioneering development of digital imaging fluorescence microscopy that permitted observation of dynamic movement of molecules in living cells in real time. (umassmed.edu)

This drawback can be overcome by fluorescence lifetime imaging (FLIM) based on time-correlated single-photon counting. (jneurosci.org)
Here, we demonstrate the applicability of rapidFLIM, combined with new and improved correction schemes, for spatiotemporal fluorescence lifetime imaging of low-emission fluorophores in a biological system. (jneurosci.org)
And the time-correlated single photon counting (TCSPC) unit of the FluoroMax-C can measure the time-resolved fluorescence lifetime of emitting materials. (horiba.com)
2D arrangement of 44,400 light stopwatches enables scan-less fluorescence lifetime imaging. (labmanager.com)
Now, a new study by scientists from Japan is set to revolutionize the field of fluorescence lifetime microscopy. (labmanager.com)
A way around the conventional problem is to focus on fluorescence lifetime instead of intensity. (labmanager.com)
The "fluorescence lifetime microscopy" technique leverages this phenomenon-which is independent of experimental conditions-to accurately quantify fluorescent molecules and changes in their environment. (labmanager.com)
Fortunately, in this recent study published in Science Advances , the aforementioned team of scientists developed a novel approach to acquire fluorescence lifetime images without necessitating mechanical scanning. (labmanager.com)
Thanks to its superior speed and high spatial resolution, the microscopy method developed in this study will make it easier to exploit the advantages of fluorescence lifetime measurements. (labmanager.com)

The mobility of a fluorescent protein fusion can be assessed using a technique known as fluorescence recovery after photobleaching (FRAP). (microscopyu.com)
Fluorescent protein fusions are the tags of choice for use in FRAP because they can be bleached without detectable damage to the cell. (microscopyu.com)
We use a number of model systems (pituitary cells, cell lines, transgenic animals) to study signal pathways for a hypothalamic peptide hormone, TRH, which acts via a G protein coupled receptor to increase cytoplasmic calcium and protein kinase C activity. (rochester.edu)
Excluding genetic constructs cloning, cell culturing and transfection, the whole protocol may take anywhere from 10 min to several hours, depending on motility of the protein being studied. (nih.gov)
We analyzed the organization and function of mitochondrial DNA in a stable human cell line (ECV304, which is also known as T-24) containing mitochondria tagged with the yellow fluorescent protein. (biomedcentral.com)
We now examine the organization of mitochondrial genomes in a human cell line that contains mitochondria tagged with the yellow fluorescent protein (YFP). (biomedcentral.com)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
Working with experts in protein chemistry, genetics, beta-lactamase biochemistry, cell wall synthesis, and medical microbiology - in Belgium, France, the United States, and Sweden - Jacobs-Wagner discovered a regulatory protein that can sense the peptidoglycans that accumulate when antibiotics disrupt cell wall synthesis, and then activate transcription of beta-lactamase, the enzyme that disarms the drugs. (the-scientist.com)
The cerebrospinal fluid had a leukocyte level of 252 cells/mm 3 (reference range 0-5 cells/mm 3 ) and a protein level of 96 mg/dL (reference range 15-45 mg/dL). (cdc.gov)
2, 3, 4, 5] BLM encodes 1417 amino acids that code for a protein in the nuclear matrix of growing cells, which is a member of the RecQ family of helicases. (medscape.com)
actin is a ubiquitous contractile protein that can be found in non-muscle cells. (medscape.com)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Nanowerk News ) Scientists can detect the movements of single molecules by using fluorescent tags or by pulling them in delicate force measurements, but only for a few minutes. (nanowerk.com)
Scientists can tune the color of the fluorescence from carbon nanodots by modifying its size and surface chemistry. (horiba.com)
Christine Jacobs-Wagner's studies of a bacterial species have changed how scientists think about cell shape and polarity. (the-scientist.com)
Fluorescence microscopy is widely used in biochemistry and life sciences because it allows scientists to directly observe cells and certain compounds in and around them. (labmanager.com)
NIDCR scientists used fluorescence microscopy to visualize pain signaling in mice's facial nerves. (medlineplus.gov)

New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. (cshlpress.com)

Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for several voltage-gated sodium channel subtypes including Nav1.2, Nav1.5, and Nav1.7. (frontiersin.org)
In summary, the Swarm is a novel high-throughput all-optical system capable of collecting information-dense data from optogenetic assays in both heterologous and iPS cell-derived models, which can be leveraged to drive diverse therapeutic discovery programs for nervous system disorders and other disease areas involving excitable cells. (frontiersin.org)

As GCaMP is approximately isosbestic at 405 nm (i.e. fluorescence is independent of calcium concentration) this gives a calcium sensitive signal and a calcium insensitive signal that can be used to control for movement artefacts 5 . (nature.com)
To address this challenge, we developed the Swarm TM , a custom designed optical instrument for highly parallelized, multicolor measurements in excitable cells, simultaneously recording changes in voltage and calcium activities at high temporal resolution under optical stimulation. (frontiersin.org)
As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
It works by affecting the movement of calcium into the cells of the heart and blood vessels. (seagullindia.com)
These results suggest that CD-349 is a more potent calcium antagonist for decreasing pacemaker activity of rabbit SA node cells as compared with the other three calcium antagonists tested. (seagullindia.com)
Verapamil HCl is a calcium ion influx inhibitor (slow-channel blocker or calcium ion antagonist) that exerts its pharmacologic effects by modulating the influx of ionic calcium across the cell membrane of the arterial smooth muscle as well as in conductile and contractile myocardial cells. (seagullindia.com)

The basic principle of fluorescence imaging is the same for all microscopes: The sample is first chemically labelled with fluorescent molecules called fluorophores. (mpg.de)

The dynamic remodeling of actin filaments (F-actin) concentrated at the cell periphery is responsible for cell translocation, for cell shape changes, and for cellular resistance to potentially disruptive mechanical stresses. (rupress.org)

Fluorescence microscopy is an essential tool for biology. (jneurosci.org)
Cell biology approaches, combined with the superb genetics of yeast, have advanced our understanding to CME. (biorxiv.org)
Present address: Department of Cell and Developmental Biology, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain. (biologists.com)
Now there's a whole new field of people who are working on bacterial cell biology using the same sorts of approaches used to study eukaryotes. (the-scientist.com)
Development: For advances in developmental biology and stem cells. (lu.se)
One team studies the biology of sensations such as pain, and the other has expertise in cell imaging. (medlineplus.gov)
Dr. Yamada, who is Chief of the NIDCR Cell Biology Section, has expertise in developmental biology. (medlineplus.gov)

Cell Therapy for Parkinson's Disease: What Next? (lu.se)

These findings support that Nurr1 exerts a protective effect on adult mDA neurons in a cell-autonomous manner Indeed, several intrinsic mechanisms associated with Nurr1-mediated cell survival have been identified. (justia.com)

Fluorescence imaging is an indispensable method for analysis of diverse cellular and molecular processes, enabling, for example, detection of ions, second messengers, or metabolites. (jneurosci.org)
The technique enabling the on-the-flow detection of the pathogens is termed spatially modulated fluorescence detection and delivers high signal-to-noise discrimination without precision optics. (parc.com)

It can do several things: measure steady-state fluorescence intensity and spectrum in a wide spectral range, from visual to near-infrared wavelengths. (horiba.com)

Surface functions during mitosis I: phagocytosis, pinocytosis and mobility of surface bound Con A. Cell 15: 327-341. (uconn.edu)

A third class of small molecule fluorophore is that of the transition metal-ligand complexes, which display molecular fluorescence from a metal-to-ligand charge transfer state which is partially forbidden, these are generally complexes of Ruthenium, Rhenium or Osmium. (wikipedia.org)
The long-term goal is to understand signal transduction in endocrine cells at a molecular and cellular level. (rochester.edu)
Fred perfected the use of the single smooth muscle cell preparation to study virtually every aspect of smooth muscle physiology and molecular anatomy at the cellular and subcellular level. (umassmed.edu)
Her pioneering studies on the molecular mechanisms underlying cell shape and cell polarity in Caulobacter crescentus , says Errington, "have helped change the way people think about bacteria. (the-scientist.com)

Analysis of simulated and experimental fluorescence recovery after photobleaching. (microscopyu.com)
fluorescence intensity is significantly affected by both experimental conditions and the concentration of the fluorescent substance. (labmanager.com)

Altogether, we show that LAMP1 + TRAIL + astrocytes limit CNS inflammation by inducing T cell apoptosis, and that this astrocyte subset is maintained by meningeal IFNγ + NK cells that are licensed by the microbiome. (nature.com)

C , Flies expressing GCaMP6s and CsChrimson in dFB neurons (R23E10-Gal4) were imaged using a 2-photon microscope, with ROIs selected for cell bodies, dendrites, and axons (dFB). (jneurosci.org)
F , Flies expressing GCaMP6s and CsChrimson in EB R3m neurons (R28D01-Gal4), with ROIs selected for cell dendrites, axons (EB), and cell bodies. (jneurosci.org)
Cell 137: 47-59) has identified an unexpected effect of Nurr1 which is expressed in glial cells in response to stimuli which trigger inflammation, and this Nurr1 suppresses the production of pro-inflammatory cytokines that cause the death of mDA neurons, but does not suggest a therapeutic effect through induction of Nurr1 expression. (justia.com)
In particular, glial cells in nerve tissues include astrocytes and microglial cells and are accessory cells that aid in promoting the functions and survival of neurons. (justia.com)
However, under a pathologic condition, the glial cells are instead activated to set up an environment in which the glial cells cause damage to neurons (M1 activation). (justia.com)
Meanwhile, it is known that glial cells with an M1 phenotype which set up a diseased environment can be converted into glial cells with an M2 phenotype which promote a therapeutic environment in which the survival and regeneration of neurons is set up. (justia.com)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)
Radiographic distinction enhancement brain tissues studies have revealed signiп¬Ѓcant inп¬Ѓltrates of tumor cells outdoors the contrasted tumor. (ehd.org)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
This can be particularly useful for studying the distribution and movement of molecules within cells or tissues. (microbiologynote.com)

People knew there was a connection between enzyme induction and cell wall breakdown," he says. (the-scientist.com)

The ability to measure over long time scales opens the possibility to study systems with strong affinity at the single-molecule limit, such as lectin-carbohydrate interactions responsible for cell recognition and adhesion," Hafner said. (nanowerk.com)
Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3. (ppjonline.org)

J Cell Biol (2007) 179 (5): 1011-1025. (rupress.org)
J. Cell Biol. (uconn.edu)
Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)

The motorized external phase-contrast system enables users to combine phase contrast with epi-fluorescence imaging without compromising fluorescent light transmission by bypassing the need to use phase-contrast objectives. (nikon.com)

Fluorescence is used in the life sciences generally as a non-destructive way of tracking or analysing biological molecules. (wikipedia.org)
The new approach has provided a way to visualise biological processes, like cell division within the heart, which have never been seen before. (ed.ac.uk)
The broader applications will enable new insights into a wide range of biological mechanisms, especially where movement and changes of shape have previously made long term imaging extremely challenging. (ed.ac.uk)
Practitioners introduce these nano-sized materials into biological cells to color the cells and track biological components. (horiba.com)
Fluorescence microscopy is a workhorse technique in biological sciences allowing specific biological structures to be labeled and imaged with high contrast. (photometrics.com)

An analytical technique based on fluorescence quenching of CdTe/CdS/ZnS quantum dots (QDs) was developed to quantify verapamil in commercially available preparations. (seagullindia.com)
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed in the early 1980s. (leica-microsystems.com)
The receptor is normally avoided by This technique from time for the cell surface area, where in fact the receptor could remove even more LDL particles. (researchensemble.com)
The technique they used―called "fluorescence microscopy"―allowed the research team to watch this happen in real time as the individual cells in the mice's facial nerves responded to each of the stimuli. (medlineplus.gov)

The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely proportional). (wikipedia.org)

These cell- and when occurring in the lung or brain lead to to-cell connections allow for the formation of serious consequences related to impaired gas inter-endothelial gaps through which substances exchange and cerebral function, respectively. (bvsalud.org)

Astrocytes are glial cells that are abundant in the central nervous system (CNS) and that have important homeostatic and disease-promoting functions 1 . (nature.com)
Specifically, a method of converting glial cells from an M1 phenotype to an M2 phenotype, wherein Nurr1 and Foxa2 are introduced into the glial cells to be overexpressed in the glial cells and a method of preventing or treating an inflammatory neurologic disorder, which includes glial cells into which Nurr1 and Foxa2 are introduced, or a viral vector loaded with Nurr1 and Foxa2, are provided. (justia.com)
Therefore, the present inventors have found that various neurologic disorders based on inflammation may be treated since transcription factors Nurr1 and Foxa2 interact with each other to convert glial cells from an M1 phenotype to an M2 phenotype which sets up a therapeutic environment, when the transcription factors are overexpressed in the glial cells. (justia.com)

It has roles in cell division, membrane trafficking, and cell migration. (biologists.com)

Because of its fluorescence properties, the sample re-emits part of the captured radiation while still preserving the aforementioned frequency-position correspondence. (labmanager.com)

The above techniques can be combined with computational methods to estimate staining levels without staining the cell. (wikipedia.org)
Microtubule membrane interaction fluorescence techniques. (uconn.edu)

Fiber photometry is the process of recording bulk neural activity by measuring fluorescence changes in activity sensitive indicators such as GCaMP through an optical fiber. (nature.com)
It aims to build a robot-human closed loop system to alter the neural control of movement as a way to rehabilitate, assist, and enhance human motor control and learning capabilities. (washington.edu)
This course is an introductory design course in Neurobotics focusing on learning about human neural control of movement, using physiological signals as inputs, and controlling a mechanical device. (washington.edu)
CSE 529: Neural Control Of Movement: A Computational Perspe Systematic overview of sensorimotor function on multiple levels of analysis, with emphasis on the phenomenology amenable to computational modeling. (washington.edu)

Latrunculin B (LatB) treatments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). (ppjonline.org)

During CME, cells deform the plasma membrane locally and subsequently pinch off a vesicle into the cytoplasm. (biorxiv.org)

The membrane potential of the red blood cells was determined before and after cell shrinkage as the external potassium concentration at which there was no change in diSC35 fluorescence. (cdc.gov)

This precision allows researchers to study individual heart cells continuously forming and dividing over a full-day period, without causing any harm to the fish. (ed.ac.uk)
Researchers can track the component's locations and movements with specific and predetermined carbon nanodots, which fluoresce at different colors. (horiba.com)
Researchers have identified certain singlet oxygen species as being able to damage a cancer cell. (horiba.com)
This limit was regarded as insurmountable − until researchers developed super-resolution fluorescence microscopy methods, such as STED and PALM / STORM, within the past two decades. (mpg.de)

Other single-molecule methods based on fluorescence are limited by photo bleaching, and those based on force measurements are limited by radiation damage and mechanical instabilities. (nanowerk.com)

In practice, the fluorescently labeled molecules in a small region of the cell are irreversibly photobleached using high laser power, followed by monitoring the subsequent movement of the surrounding non-bleached fluorescent molecules into the photobleached region using low laser power. (microscopyu.com)

Stem Cell Reports. (lu.se)

Live-cell super resolution analysis of endocytosis was also performed in fission yeast (Arasada, 2018). (biorxiv.org)
High content imaging is primarily concerned with the automated analysis of large cell populations where the goal is to process as many cells as possible in the fastest time with the highest resolution. (photometrics.com)

Genetics and live-cell imaging revealed core CME spatiodynamic similarities between the two yeasts, though two-zone actin assembly is a fission yeast-specific mechanism, which is not essential for CME. (biorxiv.org)

TRAIL expression in astrocytes is repressed by molecules produced by T cells and microglia in the context of inflammation. (nature.com)
Microglia movement in zebrafish. (bruker.com)

higher doses are 'off-label' Cd accumulation in WRL-68 cells is a time-, temperature- and concentration-dependent process. (seagullindia.com)

By decoupling the training corpus from the cells under investigation, these approaches provide an avenue for using stains that are otherwise incompatible with live cell imaging, such as anti-body staining. (wikipedia.org)
The QImaging CCD family of scientific cameras are designed with solutions for electrophysiology, long stare, color imaging, documentation and live cell imaging. (photometrics.com)
A short-term position at Oxford Brookes just after my PhD allowed me to expand my expertise to ER and Golgi as well as acquiring skills in live cell imaging. (brookes.ac.uk)
This allows high-speed and high-sensitivity live-cell imaging, enabling the best use to be made of the Ti2's large FOV. (nikon.com)

Nevertheless, if the PCSK9 molecule is normally obstructed, LDL receptors are recycled, came back to the top of cells, and remove even more LDL contaminants from flow. (researchensemble.com)
For example, narcotic drugs like opioids attach to certain receptors in brain cells. (medlineplus.gov)

Anatomy46

Organisms13

Diseases1

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Psychiatry and Psychology9

Phenomena and Processes72

Disciplines and Occupations4

Technology, Industry, Agriculture5

Information Science11

Health Care2

Time-lapse2

Resonance energy t2

Measure fluorescence2

Confocal1

Microscopy techniques2

Membranes7

Proteins10

Spectroscopy4

Excitation4

Illumination6

Quantitative1

Intracellular1

Microtubules1

Molecules in livin1

Lifetime9

Protein11

Scientists6

Mammalian cells1

Assays3

Calcium6

Fluorophores1

Concentrated at the cell periphery1

Biology7

Therapy for Parkinson's Disease1

Intrinsic1

Detection2

Wavelengths1

Mobility1

Molecular4

Experimental2

Subset1

Neurons8

Tissues5

Enzyme1

Interactions2

Biol3

Phase contrast1

Biological5

Technique4

Principle1

Endothelial1

Glial cells3

Migration1

Frequency1

Techniques2

Neural4

Treatments1

Cytoplasm1

Membrane potential1

Researchers4

Measurements1

Fluorescent molecules1

Stem1

Analysis2

Actin1

Microglia2

Concentration1

Live cell4

Receptors2