• In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. (abcam.com)
  • Fluorescein-DHPE (N-(Fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a useful surface pH indicator (1) and has also been used for measuring lateral diffusion in membranes via fluorescence photobleaching recovery method. (biotium.com)
  • AK-FLUOR® (Fluorescein Injection, USP) is a sterile solution in Water for Injection, of Fluorescein prepared with the aid of Sodium Hydroxide. (rxlist.com)
  • AK-FLUOR® (fluorescein injection) is used intravenously as a diagnostic aid. (rxlist.com)
  • A syringe, filled with fluorescein, is attached to transparent tubing and a 25 gauge scalp vein needle for injection. (rxlist.com)
  • If the needle has extravasated, the patient's blood will be seen to bulge the skin and the injection should be stopped before any fluorescein is injected. (rxlist.com)
  • When assured that extravasation has not occurred, the room light may be turned off and the fluorescein injection completed. (rxlist.com)
  • Care must be taken to avoid extravasation during injection as the high pH of fluorescein solution can result in severe local tissue damage. (rxlist.com)
  • The ocular structures impregnated by fluorescein are illuminated by a blue radiation which makes them luminescent. (vin.com)
  • Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing biologists to target the fluorophore to specific proteins or structures within cells. (wikipedia.org)
  • the released fluorescein will fluoresce , and can be detected with a microscope using filter s that allow only the longer-wavelength light to pass. (everything2.com)
  • Fluorescein can also be conjugated to nucleoside triphosphates and incorporated into a probe enzymatically for in situ hybridisation. (wikipedia.org)
  • In laboratory science, fluorescein can be used for protein labeling, and in a number of other tasks. (wisegeek.com)
  • In addition to providing data about current flow, the movement of the fluorescein can also be used to trace contaminants, by tagging the contaminant with the compound and watching to see where it goes. (wisegeek.com)
  • Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water). (wikipedia.org)
  • Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. (abcam.com)
  • Avidin D, Fluorescein labeled, having an excitation at 495 nm and an emission at about 515 nm, is made from our highly purified Avidin D by a special labeling technique. (vectorlabs.com)
  • One of its more recognizable uses was in the Chicago River, where fluorescein was the first substance used to dye the river green on St. Patrick's Day in 1962. (wikipedia.org)
  • Fluorescein can also be used to find foreign bodies such as grit in the eye, as they show up surrounded by a green ring. (netdoctor.co.uk)
  • Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining. (vectorlabs.com)