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Ligandsfms-Like Tyrosine Kinase 3CD40 LigandFas Ligand ProteinTandem Repeat SequencesLeukemia, Myeloid, AcuteReceptor Protein-Tyrosine KinasesProtein BindingRANK LigandBinding SitesSignal TransductionMolecular Sequence DataMembrane ProteinsModels, MolecularAmino Acid SequenceMutationMembrane GlycoproteinsCD30 LigandTNF-Related Apoptosis-Inducing LigandProtein Structure, TertiaryCell LineCells, CulturedDideoxynucleosidesMice, Inbred C57BLOX40 LigandReceptors, Cell SurfaceKineticsProto-Oncogene ProteinsProtein ConformationRecombinant ProteinsCell Line, TumorBinding, CompetitiveCrystallography, X-RayDendritic CellsApoptosisGene DuplicationStructure-Activity Relationship4-1BB LigandFlow CytometryLeukemia, MyeloidTransfectionHematopoietic Stem CellsCell DifferentiationMolecular StructureRecombinant Fusion ProteinsSTAT5 Transcription FactorGene Expression Regulation, LeukemicStem Cell FactorVascular Endothelial Growth Factor Receptor-1PhosphorylationRNA, MessengerBase SequenceCHO CellsTumor Cells, CulturedMice, KnockoutAntigens, CDAntigens, CD95Programmed Cell Death 1 Ligand 2 ProteinBone Marrow CellsPhenylurea CompoundsCell ProliferationRadioligand AssayReceptors, ImmunologicCarrier ProteinsIndolesPeptidesReceptors, TNF-Related Apoptosis-Inducing LigandBenzothiazolesCricetinaeMutagenesis, Site-DirectedMice, Inbred BALB CCarbazolesReverse Transcriptase Polymerase Chain ReactionT-LymphocytesReceptors, G-Protein-CoupledReceptors, Cytoplasmic and NuclearLymphoid Progenitor CellsSequence Homology, Amino AcidLymphocyte ActivationProtein Kinase InhibitorsAntibodies, MonoclonalDose-Response Relationship, DrugCell DivisionIntercellular Signaling Peptides and ProteinsBlotting, WesternCell AdhesionStaurosporineGene Expression RegulationProto-Oncogene Proteins c-kitAntineoplastic AgentsCell MembraneCOS CellsUp-RegulationThermodynamicsProteinsGene ExpressionReceptors, sigmaReceptors, NotchMice, TransgenicModels, Biological
Flt3LThrombopoietinReceptor tyrosiDendritic cellsProgenitor cellsHematopoietic cellsDifferentiationAntibodyMutationsProteinSpecificityCDNABindsCytokineInternal tandem duplicationGenesInhibitorsPrognosisTumorsRecognizesTransmembraneQuizartinibProgenitorsHighly purifiedSignificantlyMiceCellsSurvivalGeneHumanActivityMultipleEarlyImageShowSignal
Flt3L5
  • We have shown that administration of Fms-like tyrosine kinase 3 ligand (Flt3L) significantly increases in the number of dendritic cells, and synergizes with radiation therapy to improve survival in preclinical models of lung cancer. (elsevierpure.com)
  • Rabbit anti Human Flt3 Ligand antibody recognizes the ubiquitously expressed human growth factor Flt3-Ligand (Flt3L), a 235aa type I transmembrane glycoprotein which regulates the proliferation of early haematopoietic cells. (bio-rad-antibodies.com)
  • Flt3L exists in both a membrane-bound and proteolytically cleaved soluble isoform, both of which are biologically active, and initiates cell proliferation/ differentiation signalling in co-operation with other colony stimulating factors (CSFs) and interleukins, by binding to and activating the tyrosine kinase receptor Flt3 (FLT3R), expressed highly by hematopoietic progenitor cells and stem cells. (bio-rad-antibodies.com)
  • To date, it has not been elucidated if fms-like tyrosine kinase-3 (Flt3) and its natural ligand (Flt3L) are expressed in astrocytic tumors, although some of the clinically intended small-molecule receptor tyrosine kinase inhibitors affect Flt3, while others do not. (altmetric.com)
  • In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
Thrombopoietin4
  • 6. Kuter D.J., Rosenberg R.D. The reciprocal relationship of thrombopoietin (c-Mpl ligand) to changes in the platelet mass during busulfan-induced thrombocytopenia in the rabbit // Blood. (medline.ru)
  • CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells // Blood. (medline.ru)
  • Through such studies, c-kit ligand (KL), flt3 ligand (FL), thrombopoietin, and IL-11 have emerged as likely positive regulators of HSC self-renewal. (lu.se)
  • Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. (bvsalud.org)
Receptor tyrosi2
  • It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. (biolegend.com)
  • FLT3 (FMS-related tyrosine kinase 3) located on chromosome 13q12.2 encodes a receptor tyrosine kinase (RTK) that activates the Ras and PI3 kinase pathway leading to the increased proliferation and inhibition of apoptosis in hemopoietic progenitor cells [ 7 ]. (hindawi.com)
Dendritic cells4
  • Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. (rndsystems.com)
  • This therapy is based on recruitment of Flt3-positive dendritic cells, but may be corroborated by activity of this signaling pathway in glioma cells. (altmetric.com)
  • Flt3-ligand (Flt3-L), a hematopoietic growth factor, dramatically increased the number of dendritic cells in the lungs of Ova-sensitized and challenged mice. (creighton.edu)
  • Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)
Progenitor cells3
  • After irradiation-induced thymic damage, thymic recovery is facilitated by Flt3 ligand (FL), expressed by perivascular fibroblasts surrounding the thymic entry site of Flt3 receptor-positive progenitor cells. (ox.ac.uk)
  • Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. (biolegend.com)
  • Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells. (biolegend.com)
Hematopoietic cells3
  • FLT3 ligand is a growth factor that regulates the proliferation of early hematopoietic cells. (biofargo.com)
  • The FLT3 ligand itself does not stimulate the proliferation of early hematopoietic cells, but rather synergistically induces growth and differentiation with other CSFs and interleukins. (biofargo.com)
  • CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. (biolegend.com)
Differentiation2
  • FLT-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. (topsan.org)
  • Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. (rndsystems.com)
Antibody2
  • These mice received three i.p. injections of anti CD25 antibody (PC61) (250 μg) and Flt3-L (3μg) daily for ten days. (creighton.edu)
  • Western blot analysis of extracts from BaF3/FLT3 cells, untreated or stimulated with FLT3 Ligand (FL) (3 µg/ml for 5 minutes), using Phospho-FLT3 (Tyr591) Antibody (upper) or FLT3 (8F2) Rabbit mAb #3462 antibody (lower). (cellsignal.com)
Mutations2
  • The treatment paradigm of AML patients harboring FLT3 mutations (30%) has been modified by the discovery of tyrosine kinase inhibitors. (dovepress.com)
  • Activity of these inhibitors depends on their mechanism of receptor binding (active vs inactive conformation) and efficacy against the FLT3-ITD and -TKD mutations (type 1 inhibitors are active both on FLT3 -ITD and TKD, whereas type 2 inhibitors are active only on FLT3 -ITD). (dovepress.com)
Protein1
  • Product Information Protein sequence A DNA sequence encoding the human 4-1BB Ligand (NP_003802.1) was expressed with a Fc-tag at the C-terminus. (biofargo.com)
Specificity1
  • First- and second-generation inhibitors classify FLT3 inhibitors according to FLT3 specificity: first-generation FLT3 inhibitors include sorafenib and midostaurin and second-generation inhibitors are represented by quizartinib, gilteritinib and crenolanib, among others. (dovepress.com)
CDNA2
  • Be the first to review Cyno Monkey Flt-3 Ligand (XP_005589968.1) VersaClone cDNA and earn rewards! (rndsystems.com)
  • Sets of BALB/c mice had been nasally immunized every week for three consecutive weeks with OVA and a combined mix of plasmid encoding the Flt3 ligand cDNA (pFL) and CpG oligodeoxynucleotides (CpG ODN) (solid design), pFL only (striped design), or CpG ODN only (open design) as nose adjuvants. (institutopsicofarmacologia.com)
Binds1
Cytokine2
  • Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. (rndsystems.com)
  • Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. (rndsystems.com)
Internal tandem duplication3
  • Approximately 30% of the adult cases harbor an internal tandem duplication ( FLT3 -ITD) and 5- 10% a tyrosine kinase domain (TKD) amino acid substitution ( FLT3-TKD ). (dovepress.com)
  • The oncogene activation of FLT3 in hematological malignancies is mainly manifested through internal tandem duplication which may result in a poor prognosis [ 8 ]. (hindawi.com)
  • Quizartinib is a first-in-class tyrosine kinase inhibitor approved for newly diagnosed FLT3 internal tandem duplication-positive (ITD+) acute myeloid leukemia (AML). (medscape.com)
Genes3
Inhibitors1
  • The FLT3 inhibitors sorafenib, midostaurin, quizartinib and gilteritinib have been tested in monotherapy in several settings including refractory or relapsed AML (R/R AML), post-transplant maintenance as well as in combination with intensive chemotherapy (ICT) or non-intensity regimens. (dovepress.com)
Prognosis1
Tumors1
  • Genomic amplification of FLT3 has been reported in solid tumors including colorectal cancer, breast cancer, and gastric cancer [ 9 ]. (hindawi.com)
Recognizes1
  • NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. (thermofisher.com)
Transmembrane1
  • Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. (rndsystems.com)
Quizartinib2
  • Gilteritinib in combination with hypomethylating agent as well as quizartinib are not supported by solid randomized trial results for their use in FLT3-mutated AML patients. (dovepress.com)
  • Quizartinib and its active metabolite (AC886) inhibit FLT3 kinase activity, preventing autophosphorylation of the receptor, thereby inhibiting downstream FLT3 receptor signaling and blocking FLT3-ITD-dependent cell proliferation. (medscape.com)
Progenitors2
  • flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes. (topsan.org)
  • Flt3 ligand is critically involved in generation of early and common lymphoid progenitors but not in the regulation of the size of the hematopoietic stem cell pool. (ox.ac.uk)
Highly purified1
  • Antisera to human Flt3-Ligand were raised by repeated immunisations of rabbits with highly purified antigen. (bio-rad-antibodies.com)
Significantly3
  • Flt3-L significantly decreased levels of BALF IL-5, IFN-γ, eosinophilia and substantially increased IL-10 and the number of CD4+CD25+Foxp3+IL-10+ T-cells in the lung. (creighton.edu)
  • Flt3-L significantly decreased the expression of cell surface receptor CD62L, increased expression of cell surface receptor ICOS and Foxp3 transcripts in CD4+CD25+ T-cells isolated from lungs of CRA-sensitized and challenged mice. (creighton.edu)
  • Flt3-L significantly inhibited the effect of CRA sensitization to decrease GATA3 expression in lung CD4+CD25+ T-cells. (creighton.edu)
Mice1
  • Therefore, the effect of Flt3-L on CD4+CD25+ T cells in cockroach-sensitized and challenged mice was examined. (creighton.edu)
Cells4
  • Alone, flt-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. (topsan.org)
  • Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. (rndsystems.com)
  • Unlike SCF, FLT3 ligand has no effect on mast cells. (biofargo.com)
  • Whether intrathymic FL-Flt3 interactions play a role in steady-state replenishment of T cells remains unknown. (ox.ac.uk)
Survival1
  • Although the impact of FLT3 -ITD mutation is unclear in the context of venetoclax-azacitidine treatment, 6 the negative impact of FLT3 -ITD on survival of AML patients treated with ICT is well established. (dovepress.com)
Gene2
  • The current study attempts to demonstrate the existence of double minute chromosomes via FLT3 gene amplification in a patient diagnosed with chronic myelomonocytic leukemia (CMML). (hindawi.com)
  • Here, to our best knowledge, we present the first case of amplification encompassing the FLT3 gene acting as dmin in a patient with chronic myelomonocytic leukemia (CMML). (hindawi.com)
Human2
  • Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. (rndsystems.com)
  • Human and mouse Flt-3 Ligand show cross-species activity. (rndsystems.com)
Activity1
  • These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. (guidetopharmacology.org)
Multiple1
  • Multiple subtypes of FLT3-ligands have been identified. (biofargo.com)
Early1
  • Flt3 ligand-receptor interaction is important for maintenance of early thymic progenitor numbers in steady-state thymopoiesis. (ox.ac.uk)
Image1
  • Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. (guidetopharmacology.org)
Show2
  • Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation. (rndsystems.com)
  • The analytes IL-1β, IL-7, Flt-3 Ligand, and IL-12p70 show the largest variation (up to fourfold) between the age groups. (nih.gov)
Signal1