Flow cytometry k562 cells. Medical search. Frequent questions

Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
Flow cytometry was used to analyze the changes of cell cycle and apoptosis at 24 and 48 hours. (bvsalud.org)
Flow cytometry results showed that with the apoptosis rate of K562 cells was the highest when treated with 200 µg/ml DPSS for 48 h. (bvsalud.org)
CONCLUSION: DPSS can induce apoptosis through cell cycle arrest, inhibit the proliferation of K562 cells, and induce K562 cells to differentiate into monocytes, which has a potential anti-leukemia effect. (bvsalud.org)
The cell apoptosis was detected using flow cytometry. (lookformedical.com)
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. (thermofisher.com)

In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)
To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
TPA is also commonly used together with ionomycin to stimulate T-cell activation, proliferation, and cytokine production, and is used in protocols for intracellular staining of these cytokines. (wikipedia.org)
The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. (exbio.cz)
DPSS) on proliferation and differentiation of K562 cells and its related mechanism. (bvsalud.org)
METHODS: MTT assay was used to detect the effects of DPSS at 0, 25, 50, 100 and 200 µg/ml on the proliferation of K562 cells at 24, 48 and 72 hours. (bvsalud.org)
RESULTS: The proliferation of K562 cells treated with different concentrations of DPSS was inhibited in a time-dose dependent manner (r=-0.96). (bvsalud.org)
Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. (molcells.org)
or expression was down-regulated as previously described.9 Cell proliferation was analyzed by 3-(4 5. (bioerc-iend.org)
This allows CD32 to function in the activation or inhibition of immune responses including degranulation, phagocytosis, ADCC, cytokine release, and B cell proliferation. (bioxcell.com)
The regulated expression and degradation of proteins are critical to all aspects of cell development and proliferation. (biomedcentral.com)
Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. (thermofisher.com)

define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)
We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
NK cells cytokine expression, signaling and cytotoxicity has been evaluated in vitro. (bmj.com)
Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
Our results indicated that ivermectin at its very low dose, which did not induce obvious cytotoxicity, drastically reversed the resistance of the tumor cells to the chemotherapeutic drugs both in vitro and in vivo . (alecomm.com)
The lack of NK cell-mediated cytotoxicity was confirmed with K562 cells as goals. (memorial2014.com)

In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. (molcells.org)

Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
Fig. 1: Split deaminases show activity in vitro and in cells. (nature.com)
The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
MTT assay was used to investigate the effect of IVM on cancer cells growth in vitro . (alecomm.com)
e Relationship between TLR2 or TLR4 appearance and percent of B7-H1-positive cells (Pearsons relationship) Appearance.Furthermore, both ligands activate or inhibit T cells in AML, based on their degree of appearance, which may be modified in vitro and in vivo simply by chemotherapy [18]. (memorial2014.com)

We therefore adapted the K562-mb15-41BBL stimulation method to large-scale clinical-grade conditions, generating large numbers of highly cytotoxic NK cells. (aacrjournals.org)
In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
Background The success and limitations of current immunotherapies have pushed research toward the development of alternative approaches and the possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. (bmj.com)
In recent studies, this linker modified analog of goniothalamin was shown to be cytotoxic against PC-3 (prostate cancer) and MCF-7 (human breast cancer) cell lines with half maximal inhibitory concentration values of 0.13 Î¼M and 2.6 Î¼M, respectively. (lookformedical.com)
Era of cytotoxic T cells T cells in the peripheral bloodstream of a wholesome donor had been isolated utilizing a Skillet T Cell Isolation Package (Miltenyi Biotec) and cultured in RPMI 1640 (Lifestyle Technology) supplemented with 10% fetal leg serum, 100?IU/ml penicillin, 100?mg/ml streptomycin, 2?mM l-glutamine, 50?M -mercaptoethanol and 20?IU/ml interleukin 2 (PeproTech, Rocky Hill, USA). (memorial2014.com)
Finally, we examined efficiency of cytotoxic T-cell activity against blast cells. (memorial2014.com)

Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
A Comparison of Reactive Oxygen Species Generation by Rat Peritoneal Macrophages and Mast Cells Using the Highly Sensitive Real-Time Chemiluminescent Probe Pholasin: Inhibition of Antigen-Induced Mast Cell Degranulation by Macrophage-Derived Hydrogen Peroxide" (PDF). (wikipedia.org)
Herein, we report a potential leukocyte differentiation antigen gene VSTM1 ( V-set and transmembrane domain-containing 1 ) that was downregulated in bone marrow cells from leukemia patients and exhibited a higher degree of promoter methylation. (biomedcentral.com)
VSTM1 ( V-set and transmembrane domain-containing 1 ) encodes a potential leukocyte differentiation antigen that is highly expressed in myeloid cells, but silenced in multiple leukemia cell lines [ 1 ]. (biomedcentral.com)
CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. (exbio.cz)
Cell differentiation antigen, CD33 and CD11b, were positively expressed after treated with DPSS. (bvsalud.org)
CD32 enables interaction between Fc γ RII-expressing cells and opsonized antigen or IgG-containing immune complexes. (bioxcell.com)

In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RC Monoclonal Antibody (Catalog # MAB22691, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). (rndsystems.com)
Unlike HIV infection, which progresses to AIDS absent suppressive anti-retroviral therapy, nonpathogenic infections in natural hosts, such African green monkeys, are characterized by a lack of gut microbial translocation and robust secondary lymphoid natural killer cell responses resulting in an absence of chronic inflammation and limited SIV dissemination in lymph node B-cell follicles. (nature.com)
Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
TPA is used to stimulate division of B-cells during cytogenetic diagnosis of B-cell cancers such as chronic lymphocytic leukemia. (wikipedia.org)
Acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) are myeloid blood cell malignancies that show great heterogeneity. (biomedcentral.com)
Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
on human chronic myeloid leukemia K562 cells. (edu.hk)

Flow cytometry, immunohistochemistry, and immunofluorescence were performed to investigate the reversal effect of IVM in vivo . (alecomm.com)
Low Endotoxin/Azide Free Anti-Mouse CD19 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and separation assays. (southernbiotech.com)

Separation of K562 cells stained using anti-human CD90 (Ber-H8) PE antibody (concentration in sample 5 μg/ml, red) from unstained K562 cells (black) in flow cytometry analysis (surface staining). (exbio.cz)
In the bioassay, compounds 1-12 dramatically reversed multidrug resistance in cancer cells with the fold-reversals ranging from 17.9 to 396.8 at the noncytotoxic concentration of 10 µM. (bvsalud.org)
Cell cycle analysis showed that with the increase of DPSS concentration, cells in G2/M phase increased (r=0.88), and cells were blocked in G2/M phase. (bvsalud.org)
Interestingly, the half maximal inhibitory concentration (IC50) of BLMs in different cancer cell lines varies from nM to μM ranges. (lookformedical.com)

MTT assay was used to evaluate cell growth inhibition. (edu.hk)
Using genetic assay and whole-genome sequencing, we found that the mutation rate could be greatly elevated in S. cerevisiae cells that underwent Zeocin (a BLM member) treatment. (bvsalud.org)
The testosterone secretion from TM3 cells was measured using the enzyme-linked immunosorbent assay. (lookformedical.com)

Conclusions These studies provide the rationale for testing expanded natural killer cells in humans. (haematologica.org)
In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. (southernbiotech.com)

This antibody was developed for use in flow cytometry. (bdbiosciences.com)
Immunofluorescent staining of FFPE human U-2 OS cells with ARL13B antibody (green) and DAPI nuclear stain (blue). (nsjbio.com)
Red=cells alone, Green=isotype control, Blue= ARL13B antibody. (nsjbio.com)
Applications Reported: This 90 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
For erythroid differentiation single-cell suspensions were immunostained with anti-TER119-PE and anti-CD71-FITC (BD Italia Milan Italy) and analyzed by flow cytometry. (bioerc-iend.org)

Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)
Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and functionality. (frontiersin.org)
The expression level of its predominant encoded product, VSTM1-v1, was positively correlated with myeloid cell maturation state. (biomedcentral.com)
A combination of CD34/CD117/CD13/CD16 staining showed that VSTM1-v1 expression in myeloid cells was positively correlated with cell maturation state. (biomedcentral.com)
Domaica, C.I. Human Natural Killer Cell Maturation Defect Supports In Vivo CD56bright to CD56dim Lineage Development. (uba.ar)
Zwirner, N.W. 'Human Natural Killer Cell Maturation Defect Supports In Vivo CD56bright to CD56dim Lineage Development' (2012) PLoS ONE. (uba.ar)
Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. (molcells.org)
Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. (southernbiotech.com)

This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
ASXL1 mutations inhibit G-CSF-induced myeloid differentiation of 32Dcl3 cells. (jci.org)

Greater than 95% knockdown of the non-catalytic subunit CSN2 and the deneddylating subunit CSN5 of the CSN was achieved in the human myeloid progenitor cell line K562. (biomedcentral.com)

In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)

Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
CD32 is expressed on monocytes/macrophages, granulocytes, platelets and B cells. (bioxcell.com)

They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
The cell surface antigens CD33 and CD11b were detected by flow cytometry. (bvsalud.org)

11. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. (southernbiotech.com)

To get insights into the specific functions of and in erythropoiesis we used animal and cultured cell models that differentially express the two isoforms. (bioerc-iend.org)
Human CD32 exists in at least six isoforms originating from three different genes (Fc γ RII A, B, and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses. (adipogen.com)

Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)
Global gene expression of Cish fl/fl Ncr1 Ki/+ NK cells compared with Cish +/+ Ncr1 Ki/+ NK cells revealed upregulation of pathways and genes associated with NK cell cycling and activation. (bmj.com)
Cell culture and gene silencing The human lymphoblast K562 cell line (ATTC number CCL-243? (bioerc-iend.org)
Specific protein knockout of immune cells, accomplished by gene editing, can be used to show how certain genes and proteins affect cell function and immune responses. (umn.edu)

Detection of IL‑17 RC in K562 Human Cell Line by Flow Cytometry. (rndsystems.com)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
Using intravenous injection of B16F10 melanoma cell line and EO711 triple negative breast cancer cell line, metastasis evaluation was performed. (bmj.com)
The values of LD50 (lethal dose 50%) calculated from experiments are different for each cell line . (lookformedical.com)

K562 cells and human primary T and NK cells were electroporated with the guides, then allowed to recover and multiply before collection for downstream assays. (umn.edu)

We viewed B7-H1 appearance in a number of myeloid lines (U937, K562, KG1a, HL60, THP-1) and lymphoid lines (Raji, Jurkat). (memorial2014.com)
a Stream cytometry evaluation of B7-H1 appearance in myeloid leukemic cell lines (LAMA-84, HL60, K562, U937, KG1a, THP-1) and lymphoid lines (Raji, Jurkat) with or without incubation with 500?IU/ml IFN- for 24?h. (memorial2014.com)

Flow cytometry was performed to analyze mitochondrial membrane potential (Δψm). (edu.hk)
Objective:To analyze the effect of high-flow nasal cannula (HFNC) oxygen therapy in patients with acute respiratory failure, and investigate the indicators that predict the failure of HFNC.Methods:The clinical data of 174 patients with acute respiratory failure were retrospectively analyzed. (bvsalud.org)

Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)

For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)

Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
BLM-treated cells showed an elevated rate of mutations, but the underlying mechanisms remained unclear. (bvsalud.org)

Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
To determine whether it plays a role in leukemogenesis, we characterized its expression pattern and function in bone marrow cells from AML/CML patients and myeloid leukemia cell lines. (biomedcentral.com)
We measured VSTM1 expression in leukemia cell lines and bone marrow biopsies from leukemia patients using qRT-PCR. (biomedcentral.com)
Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
17 Heme content was measured as described elsewhere.18 Erythropoiesis To obtain burst-forming units-erythroid (BFU-E) and colony-forming units-erythroid (CFU-E) 3 cells Linoleylethanolamide from fetal liver or adult bone marrow were cultured in MethoCult M3334 (Stemcell Technologies Vancouver Canada). (bioerc-iend.org)

In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. (exbio.cz)
CISH deletion favors NK cell accumulation to the primary tumor, optimizes NK cell killing properties and decreases TIGIT immune checkpoint receptor expression, limiting NK cell exhaustion. (bmj.com)
2015). "SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4" Cell Signal 27(1): 37-46. (bioxcell.com)
CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. (thermofisher.com)
5. Kline GH, Hayden TA, Riegert P. The initiation of B cell clonal expansion occurs independently of pre-B cell receptor formation. (southernbiotech.com)

We show that both BRD2 and BRD4 control inflammatory cytokine production in NK cells isolated from healthy volunteers and from rheumatoid arthritis patients. (frontiersin.org)
Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)
Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK cells to evaluate the impact on their functions and antitumor properties. (bmj.com)

TPA induces KSHV reactivation in PEL cell cultures via stimulation of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway. (wikipedia.org)

JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
The beads serve as a quality control for fluorochrome-conjugated monoclonal antibodies minimizing the effects of spectral spillover between multiple fluorochromes and flow cytometer channels. (novusbio.com)
Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
D ) ASXL1 protein expression using C-terminus anti-ASXL1 antibodies in leukemia cell lines. (jci.org)
Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. (thermofisher.com)

Flow Cytometry is a technique for quantifying characteristics of cells such as cell number, size and complexity, fluorescence, phenotype, and viability. (novusbio.com)
Infiltration and phenotype of NK cells in the tumor was evaluated. (bmj.com)
Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)
We conclude that CSN2 is required for CSN integrity and the stability of individual CSN subunits, and postulate that CSN2 loss results in a phenotype distinct from that of cells lacking CSN5 possibly as a consequence of altered CSN5 activity within a resultant CSN subcomplex. (biomedcentral.com)

CSN5 knockdown caused mitotic defects, G2/M arrest and apoptotic cell death. (biomedcentral.com)
CSN2 knockdown resulted in non-apoptotic cell death associated with accumulation of both the autophagy marker LC3-II and autophagic vacuoles. (biomedcentral.com)

These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)

The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
The inducibility of B7-H1 appearance by TLR or IFN- ligands shows that several stimuli, either produced through the immune system response against leukemia cells or released by infectious microorganisms, could secure leukemic cells from T cells. (memorial2014.com)

Quantification of Linoleylethanolamide dsRed+ cells from zebrafish embryos Transgenic embryos expressing green fluorescent protein (GFP) under the control of the promoter and red fluorescent protein (dsRed) under the promoter 16 were dissociated into single cells and analyzed by flow cytometry.14 O-dianisidine staining and heme content For O-dianisidine staining ten embryos/condition were collected and stained according to standard procedure. (bioerc-iend.org)
Sanger sequencing was used to determine editing efficiency, and flow cytometry was used to determine protein knockout. (umn.edu)
The results demonstrate the ability of the SHC014 surface protein to bind and infect human cells, validating concerns that this virus-or other coronaviruses found in bat species-may be capable of making the leap to people without first evolving in an intermediate host, Nature reported. (alecomm.com)

Wright-Giemsa staining was used to observe the morphological changes of K562 cells. (bvsalud.org)
Morphological observation showed that the K562 cell body increased, the amount of cytoplasm increased, the ratio of nucleus to cytoplasm decreased, and the nuclear chromatin was rough after DPSS treatment. (bvsalud.org)
Zebrafish embryos were maintained according to standard procedures.14 Embryos were staged using hours post-fertilization (hpf) and morphological criteria.15 Zygotes were collected at one-cell stage and injected with 4 ng of oligomorpholino in the presence of phenol red for subsequent selection. (bioerc-iend.org)

These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). (rndsystems.com)

The development of clinically applicable methods to produce large numbers of fully functional NK cells is a critical step to maximize the potential of this approach. (aacrjournals.org)
A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. (frontiersin.org)

Translesion-synthesis polymerases are crucial for the viability of Zeocin-treated yeast cells at the sacrifice of a higher mutation rate. (bvsalud.org)

1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
We used peripheral blood mononuclear cells from Q27W FcgammaRIIa patients and healthy controls, and cultured cells that overexpress the Q27W and common FcgammaRIIa variants. (bioxcell.com)

MEK inhibitors, including AZD6244 and UO126, reduced B7-H1 appearance and restored CTL-mediated lysis of blast cells. (memorial2014.com)

Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
Cish fl/fl Ncr1 Ki/+ NK cells display lower activation thresholds and Cish fl/fl Ncr1 Ki/+ mice are more resistant to tumor metastasis and to primary breast cancer growth. (bmj.com)

Therefore, we speculated that VSTM1-v1 expression might be associated with the maturity of myeloid cells. (biomedcentral.com)

Restoration of VSTM1-v1 expression inhibited myeloid leukemia cells' growth. (biomedcentral.com)
In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. (exbio.cz)
CD97 expression is upregulated on activated T cells. (bdbiosciences.com)
B ) Expression of ASXL1-WT and its mutants in 293T cells or 32Dcl3 cells transiently transfected or stably transduced with a FLAG-ASXL1-MT1, ASXL1-MT2, ASXL1-WT, or an empty vector (pMYs-IG). (jci.org)
F ) Surface expression of CD11b in 32Dcl3 cells transduced with indicated plasmid after incubation with 1 ng/ml IL-3 (blue) or 50 ng/ml G-CSF for 6 days (red) was analyzed by flow cytometry (top). (jci.org)
Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. (thermofisher.com)
Open up in another window Fig.?1 TLR and B7-H1 expression in leukemic cell lines and blast cells from AML. (memorial2014.com)

TPA is also being studied as a drug in the treatment of hematologic cancer[citation needed] TPA has a specific use in cancer diagnostics as a B-cell specific mitogen in cytogenetic testing. (wikipedia.org)

In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
Trained immunity of natural killer (NK) cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after preactivation. (cancerbiomed.org)

CD38 participates in cell adhesion, signal transduction and calcium signaling. (thermofisher.com)

Anatomy43

Organisms11

Diseases8

Chemicals and Drugs91

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Psychiatry and Psychology1

Phenomena and Processes59

Disciplines and Occupations3

Technology, Industry, Agriculture2

Information Science2

Health Care4

Apoptosis10

Proliferation15

Cytotoxicity8

Differentiation of K562 cells1

Vitro6

Cytotoxic7

Antigen7

Chronic8

Immunohistochemistry2

Concentration4

Assay3

Humans2

Antibody6

Erythroid differentiation2

Maturation8

Inhibit3

Progenitor1

Tumor cell1

Monocytes2

Antigens2

Dendritic cells1

Isoforms2

Gene5

Line4

Assays1

Lymphoid2

Analyze2

Mitochondrial1

Vivo7

Mutations3

Bone5

Receptor5

Cytokine4

Induces1

Potent1

Antibodies5

Phenotype4

Apoptotic cell2

Natural killer cell differen1

Immune6

Protein3

Morphological3

Endothelial cells1

Functional NK cells2

Viability1

Peripheral2

Inhibitors1

Resistant2

Myeloid cells1

Expression7

Cytogenetic1

Cytokines2

Adhesion1