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Flow CytometryRegional Blood FlowImage CytometryBlood Flow VelocityLaser Scanning CytometryApoptosisCells, CulturedImmunophenotypingExtracellular Signal-Regulated MAP KinasesMitogen-Activated Protein Kinase 1Cell Line, TumorMitogen-Activated Protein Kinase 3Antigens, CDCell SeparationCell ProliferationFluorescent DyesPloidiesCell CycleMAP Kinase Signaling SystemSignal TransductionCell SurvivalPulsatile FlowCell LineStaining and LabelingAntibodies, MonoclonalTime FactorsGene FlowPhosphorylationEnzyme ActivationMonocytesPropidiumBlotting, WesternMicrospheresT-LymphocytesFluorescein-5-isothiocyanateCD4-Positive T-LymphocytesCell DivisionRNA, MessengerReverse Transcriptase Polymerase Chain ReactionAntigens, CD45Microscopy, FluorescenceDose-Response Relationship, DrugTumor Cells, CulturedFluoresceinsLeukocytes, MononuclearCytokinesCell DifferentiationEnzyme InhibitorsImmunohistochemistryMicroscopy, ConfocalAnnexin A5Lymphocyte CountMitogen-Activated Protein Kinase 7FluorescenceUp-RegulationCell-Derived MicroparticlesLymphocytesLeukocytesButadienesCell CountMice, Inbred C57BLLymphocyte SubsetsLymphocyte ActivationB-LymphocytesDNA, NeoplasmEnzyme-Linked Immunosorbent AssayNeutrophilsSensitivity and SpecificityMice, Inbred BALB CPlatelet ActivationTransfectionT-Lymphocyte SubsetsRheologyCD8-Positive T-LymphocytesInterferon-gammaFluorescent Antibody TechniqueFlavonoidsCell AdhesionGreen Fluorescent ProteinsBone Marrow CellsAntigens, CD3Endothelial CellsAntineoplastic AgentsMembrane GlycoproteinsMitogen-Activated Protein Kinase KinasesGene ExpressionBiological MarkersPhenotypeBlood PlateletsCell MovementCerebrovascular CirculationCaspase 3Reproducibility of ResultsGene Expression RegulationDown-RegulationTumor Necrosis Factor-alphaAneuploidyBlood CellsAntigens, SurfaceCoronary Circulation

MAPK1Erk1 and Erk2AntibodyImmunofluorescenceMEK1KinaseTyr187IsoformSpecificityAssayProteinKinasesRolesCellsHumanCellERK1 and ERK2ELISAApoptosisChIPAnalysesCytometricActivationHuman

MAPK16

• ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. (assaysolution.com)
• B ) Feature plots showing the expression of Braf, Map2k1 (MEK1), Map2k2 (MEK2), Mapk3 (ERK1), Mapk1 (ERK2), Ksr1, and Ksr2 genes for the same cells in (A). ( C ) Violin plots showing median gene expression from (B) across cell-type clusters. (bioz.com)
• Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. (antibodies-online.cn)
• IL-33 binds to and signals through ST2 (IL-1R1) and its stimulation recruits MYD88, IRAK, IRAK4 and TRAF6, followed by phosphorylation of ERK1 (MAPK3) / ERK2 (MAPK1), p38 (MAPK14) and JNK. (adipogen.com)
• ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. (assaygenie.com)
• This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. (assaygenie.com)

Erk1 and Erk22

• Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). (lsbio.com)
• ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. (assaysolution.com)

Antibody2

• Applications Reported: The AFS98 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
• Applications Tested: The AFS98 antibody has been tested by flow cytometric analysis of peritoneal exudate cells. (thermofisher.com)

Immunofluorescence1

• We have 3206 products for the study of the Response To Uv Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits. (novusbio.com)

MEK12

• However, MEKi pre-treatment, which blocks ERK2 pT-E-pY phosphorylation and dissociation from MEK1/2, prevents ERK2 turnover. (babraham.ac.uk)
• MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. (abcam.com)

Kinase2

• Curcumin-induced paraptosis has been reported to be positively associated with ERK2 and JNK (c-jun N-terminal kinase-1) activation [ 16 ]. (hindawi.com)
• Our results suggest that ERKi, including current clinical candidates, act as 'kinase degraders', driving the proteasome-dependent turnover of their major target, ERK2. (babraham.ac.uk)

Tyr1871

• Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. (assaysolution.com)

Isoform1

• Here we show that eight different ERKi (both catERKi or dmERKi) drive the turnover of ERK2, the most abundant ERK isoform, with little or no effect on ERK1. (babraham.ac.uk)

Specificity1

• This assay has high sensitivity and excellent specificity for detection of Porcine Total ERK2. (assaysolution.com)

Assay1

• Histology, multiparameter flow cytometry, and enzyme-linked immunosorbent assay confirmed the expression of ProTα. (knoblauchpublishing.com)

Protein1

• In the human monocytic MonoMac 6 cell line, 4-HNE caused selective inhibition of the activity of the mitogen-activated protein kinases p38 and ERK1/ERK2, but not JNK. (monocyte.eu)

Kinases1

• However, in monocytes, the activities of all three kinases were inhibited, suggesting that the effects of 4-HNE were exerted at points upstream of ERK1/ERK2 and JNK as the levels of the phosphorylated kinases were reduced. (monocyte.eu)

Roles1

• In view of the roles of p38, ERK1/ERK2, JNK, and nuclear factor-kappaB in inflammation, the data suggest that 4-HNE, at nontoxic concentrations, has anti-inflammatory properties, most likely through an effect on these signaling molecules, and could lead to the development of novel treatments for inflammatory diseases. (monocyte.eu)

Cells3

• Flow cytometry analysis was employed to detect the size and granularity of HCT116 cells. (hindawi.com)
• ERKi treatment of cells drives the poly-ubiquitylation and proteasome-dependent turnover of ERK2 and pharmacological or genetic inhibition of Cullin-RING E3 ligases prevents this. (babraham.ac.uk)
• In vitro experiments using peripheral mononuclear cells and valvular interstitial cells were performed using multiparameter flow cytometry and quantitative polymerase chain reaction. (knoblauchpublishing.com)

Human1

• Recombinant fragment within Human ERK1 + ERK2 aa 150 to the C-terminus. (abcam.com)

Cell1

• Cell proliferation studies were performed by MTT assays and cell cycle analyses were performed by flow cytometry. (biomedcentral.com)

ERK1 and ERK21

• ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology. (biolegend.com)

ELISA2

• We have 3027 products for the study of the Smooth Muscle Cell Migration Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits. (novusbio.com)
• The Antibody Database also provides direct links to the appropriate Product Datasheets and offers a new approach for viewing information on antibodies that can be used for flow cytometry and other techniques such as Western Blot, Immunoprecipitation, ELISA and Neutralization. (chromocyte.com)

Apoptosis2

• Pb induced cell apoptosis was measured by flow cytometry. (molcells.org)
• Cell proliferation assay was performed by MTT Cell cycle, ROS levels and apoptosis were analyzed by flow cytometry, protein levels were detected by Western Blotting. (biomedcentral.com)

ChIP1

• Tumor cell lines were evaluated using the following tests: trypan blue exclusion test, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], flow cytometry, cytoskeleton organization analysis, cell cycle, mitochondria membrane depolarization, clonogenicity test, DNA fragmentation test and differential protein expression by HPLC-Chip/MS analysis. (biomedcentral.com)

Analyses1

• Methods: In this "test case" observational study, BAL samples from 119 donors undergoing lung cancer screening and biopsy procedures were evaluated by conventional and spectral flow cytometry to exemplify the comprehensive immune analyses possible with this biospecimen. (bvsalud.org)

Cytometric1

• For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. (biolegend.com)

Activation3

• Furthermore, sensitization could be blocked by the ERK inhibitor U0126 but not the p38 inhibitor SB203580, suggesting that activation of ERK2 is required for this effect. (nih.gov)
• 4. Leptin induces, via ERK1/ERK2 signal, functional activation of estrogen receptor alpha in MCF-7 cells. (nih.gov)
• Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. (assaygenie.com)

Human1

• For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. (nature.com)