Flavivirus detection. Medical search. Frequent questions

Since VHF share symptoms with many other diseases, positive identification of the disease relies on laboratory evidence of the viruses in the bloodstream, such as detection of antigens and antibodies or isolation of the virus from the body. (who.int)
Detection of antibodies to Toxoplasma gondii and Neospora caninum in animals from three zoos in Slovakia. (nih.gov)
The two new patent applications expand the growing intellectual property portfolio for the company and the innovative features that make this an ideal immunoassay system for the detection of antibodies to pathogens including Zika and other flaviviruses. (prnewswire.com)
He is applying this broad-based knowledge of virology and human immunology to develop assays for specific detection of SARS-CoV-2 IgG and IgM antibodies. (unc.edu)
ZIKV infection diagnosis relies on serology-which is challenging due to cross-reactions with other flaviviruses and/or absence or low titer of IgM and IgG antibodies at early phase of infection- virus isolation, which is labor intensive, time consuming and requires appropriate containment. (biomedcentral.com)
To achieve this, the researchers exposed mice to Zika and Dengue viruses and then screened the resulting antibodies, in pairs, against every flavivirus' version of the NS1 protein. (news-medical.net)
We were able to tease out the very few antibodies within the repertoire that would give you uniqueness in the detection. (news-medical.net)
The new tests allow the detection of legacy concentrations of antibodies indicative of prior infection, which are a lot lower. (prfire.co.uk)
This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. (butantan.gov.br)
The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV. (butantan.gov.br)
However, cross-reactive antibodies remain problematic, and may complicate the ability to distinguish between MVEV and other flaviviruses. (mja.com.au)
The Panbio dengue IgM Capture ELISA is for the qualitative detection of IgM antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever. (ncnsd.org)
Detection of IgM antibodies to dengue virus by ELISA is a valuable procedure, particularly in second and subsequent infections where the occurrence of complications is high. (ncnsd.org)
Groen, J., et al (2000) Evaluation of Six Immunoassays for Detection of Dengue Virus-Specific Immunoglobulin M and G Antibodies. (ncnsd.org)
Sang, C., et al (1998) Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies Produced during Dengue Infection. (ncnsd.org)
Infection enhancement of dengue type 2 virus in the human U-937 monocyte line by antibodies to Flavivirus cross-reactive determinants. (microbiologyresearch.org)

We isolated some apparently new flavivirus from duck incubated in 10-day-old SPF chicken embryos and duck embryos, and found that the isolates belonged to the genus flavivirus based on sequence analysis. (biomedcentral.com)
The virus had lower nucleotide homology with other genus of flavivirus. (biomedcentral.com)
Zika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. (butantan.gov.br)
The causative agents, enveloped single-strand RNA viruses from the genus Flavivirus with 4 serotypes, are transmitted by the bite of Aedes mosquitoes. (msdmanuals.com)
A generic nested-RT-PCR followed by sequencing for detection and identification of members of the alphavirus genus[J]. J Virol Methods, 2001,95(1/2):153-161. (bmsw.net.cn)
Z ported human infections indicated that clinical characteris- ika virus (ZIKV) is a mosquito-transmitted virus in the tics of infection with ZIKV included fever, headache, mal- family Flaviviridae and genus Flavivirus . (folkhalsomyndigheten.se)
The Flavivirus genus is composed of more than 70 arthropod-transmitted viruses, of which 30 are known to cause human disease. (medscape.com)

An outbreak of Zika virus disease, caused by a flavivirus transmitted by Aedes mosquitoes, occurred in Brazil in early 2015. (cdc.gov)
These cases were attributed to Zika virus, a flavivirus transmitted by Aedes mosquitoes, when the first cases confirmed by reverse transcription-polymerase chain reaction (RT-PCR) were reported in Bahia and Rio Grande do Norte states in April 2015 ( 4 , 5 ). (cdc.gov)
Sentinel chicken, mosquito surveillance, viral detection in mosquitoes and climate modelling are used to provide early warning of arboviral disease activity in Australia. (health.gov.au)
Flaviviruses are viruses transmitted by arthropods, mainly mosquitoes. (bvsalud.org)
In Paraguay, dengue, yellow fever, and Zika viruses were detected in human infections, but studies of flaviviruses in mosquitoes are still scarce. (bvsalud.org)
Therefore, the objective of the present study was the implementation of a flavivirus detection system in mosquitoes at IICS-UNA. (bvsalud.org)
West Nile virus is an arthropod-borne Flavivirus maintained in enzootic transmission cycles between birds and mosquitoes, but incidental infection of humans and horses can lead to neuroinvasive disease (Work et al. (ivis.org)
Saint Louis encephalitis virus (SLEV), West Nile virus (WNV) and Ilheus virus (ILHV) are flaviviruses maintained by enzootic transmission networks between mosquitoes and birds. (bvsalud.org)
We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. (unl.pt)
Detection of flaviviruses and orthobunyaviruses in mosquitoes in the Yucatan Peninsula of Mexico in 2008. (revistabiomedica.mx)
In addition a novel assay for flaviviruses conformed by a RT-qPCR coupled with a low density DNA microarray was developed, which enabled the identification of WNV in mosquitoes from Greece. (uni-greifswald.de)
Win T. Detection of Dengue viruses by immuno fluorescence of the intra cerebral inoculation of mosquitoes Lancet. (ijmedicine.com)

Zika virus is a mosquito-borne flavivirus closely related to the dengue and yellow fever viruses. (wikipedia.org)
Aravinda de Silva is a Professor in the Department of Microbiology and Immunology with expertise in the area of mosquito-borne flavivirus and dengue virus vaccines and diagnostics. (unc.edu)
Zika virus (ZIKV), a mosquito borne flavivirus is a pathogen affecting humans in Asia and Africa. (biomedcentral.com)
Takeda, having expertise in the Flavivirus vaccine field acquired through the ongoing development of a vaccine candidate for dengue, believed that this could be leveraged to support a rapid, collaborative response to the outbreak of Zika, a mosquito borne Flavivirus. (ifpma.org)
West Nile virus (WNV) is a mosquito-borne flavivirus (single-stranded RNA virus) that primarily infects birds but occasionally infects horses and humans. (testcatalog.org)
Reference Dengue is a mosquito-borne disease caused by a flavivirus. (msdmanuals.com)

DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. (ultrassay.com)

Flaviviruses include West Nile virus and the virus that causes yellow fever, as well as Dengue virus. (news-medical.net)
Blacksell, S. et al (2007) Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. (ncnsd.org)
This year, we tried to jump into as many chances as possible to learn more about role and value of Finding Flavi - dengue virus serotype prediction and detection systems. (igem.org)
He introduced existing detection methods for dengue virus to us and also mentioned both advantages and disadvantages that those methods have. (igem.org)

The RT-LAMP assay is very simple and rapid, and the amplification can be completed within 50 min under isothermal conditions at 63°C by a set of 6 primers targeting the E gene based on the sequences analysis of the newly isolated viruses and other closely related Flavivirus.The monitoring of gene amplification can also be visualized by using SYBR green I fluorescent dye. (biomedcentral.com)
Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)
The sequencing of 19/91 pools positive for flavivirus was carried out, identifying flavivirus in all cases of insects (mainly detecting Culex Flavivirus, cell fusing agents Flavivirus and Kamiti river virus), evidencing the high distribution of these viruses. (bvsalud.org)
This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research. (erau.edu)
The RNA from seven isolates of WN virus from six countries and four other flaviviruses (Kunjin, Japanese encephalitis, St. Louis encephalitis, and yellow fever viruses) was reverse- transcribed (RT) and amplified by PCR. (usuhs.edu)
Unfortunately, many other viruses from the same family, known as flaviviruses, have similar versions of NS1 and can produce a false positive. (news-medical.net)
To counteract this, the flaviviruses, like other viruses, have encoded a variety of antagonists, and use a multi-layered molecular defense strategy to establish their infections. (shengsci.com)
First isolated in monkeys in Uganda in 1947, the Zika virus is a member of the flaviviruses-a classification of viruses that also includes West Nile and dengue fever virus. (neurosciencenews.com)
Following this approach a series of assays were designed, developed or adapted and put into use for detection and characterization of important RNA viruses. (uni-greifswald.de)
One of those viruses is West Nile virus (WNV), which after its explosive introduction into USA become the most widespread flavivirus throughout the world and, consequently, many countries began an intensive monitoring. (uni-greifswald.de)
Detection and subtyping of dengue 1-4 and yellow fever viruses by means of a multiplex Rt-nested-PCR using degenerated primers[J]. Trop Med Int Health,2006,11(9):1432-1441. (bmsw.net.cn)

This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)
Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)
This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. (erau.edu)
Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. (erau.edu)
Therefore, real-time RT-PCR (rRT-PCR) is an appealing option as a rapid, sensitive and specific method for detection of ZIKV in the early stage of infection. (biomedcentral.com)
The NS5 protein coding regions of African ZIKV isolates were sequenced and aligned with representative flaviviruses sequences from GenBank to design primers and probe from conserved regions. (biomedcentral.com)
The assay was shown to detect 37 ZIKV isolates covering a wide geographic in Africa and Asia over 36 years but none of the 31 other flaviviruses tested showing high analytical specificity. (biomedcentral.com)
We have developed a rapid, sensitive and specific rRT - PCR for detection of ZIKV. (biomedcentral.com)
This assay is a useful tool for detection of ZIKV infection in regions where a number of other clinically indistinguishable arboviruses like dengue or chikungunya co-circulate. (biomedcentral.com)
Like all flaviviruses, ZIKV is a single-stranded RNA virus with a positive-polarity RNA genome of approximately 11 kb. (biomedcentral.com)
The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. (butantan.gov.br)

All 172 tissue homogenates were positive for flavivirus but in- Felix Gómez, Manuel Fernández Morente, examined were negative by this test. (cdc.gov)

Consideration of the genetic identity of both the Dengue and Zika flaviviruses as well as the cross-reactivity of relevant T cells along with the actions of CD4+ cytotoxic cells in these infections are also presented. (frontiersin.org)
Finally, PCR can be useful for supporting a serologic diagnosis, given the known cross-reactivity of WNV serology with other flaviviruses. (testcatalog.org)

The discovery of a new flavivirus (GBV-C), closely related to HCV, provided an opportunity to demonstrate the flexibility of this quantitative PCR method. (ucl.ac.uk)
The designed primers do not cross-react with louping ill virus, the most closely related flavivirus. (tbenews.com)

RNA extraction is essential for the molecular detection of common viral pathogens. (bvsalud.org)
As frequently observed for ISF, the viral genomes seems to encode a putative Fairly Interesting Flavivirus ORF (FIFO)-like product, the synthesis of which would occur as a result of a -1 translation frameshift event. (unl.pt)
Flavivirus and Filovirus EvoPrinters: New alignment tools for the comparative analysis of viral evolution. (cdc.gov)
Detection of viral sequences of low reiteration frequency by in situ hybridization. (microbiologyresearch.org)
Accuracy studies were performed by testing negative clinical specimens with whole viral genomic RNA for lineages 1 and 2 near the limit of detection (LOD) and yielded greater than or equal to 97% sensitivity and specificity. (testcatalog.org)

The two new patent applications are based on the Company's INSTI platform and describe methods and kits allowing either simultaneous or sequential detection of different antibody classes to multiple pathogens using a single sample of blood or blood product. (prnewswire.com)
Excivion's granted patents in Europe and the USA (Granted European Patent No. EP3458471B1, Granted US Patent No. 11,028,133 B2) provide composition of matter coverage for glycan-cloaked flavivirus antigens covering all positions in the fusion loop which is 100% conserved across all major human flavivirus pathogens (Zika, dengue 1-4, Japanese encephalitis virus and yellow fever virus). (prfire.co.uk)

Aedes taeniorhynchus fue capturado frecuentemente en temporada de lluvias. (revistabiomedica.mx)

Ultrassay Dengue genotyping detection kit is used for qualitative typing detection of denguevirus (DENV) nucleic acid in suspected patient's serum sample to help diagnose patients with Dengue fever. (ultrassay.com)
It contains specific primers and probes for fluorescence detection, which are designed to target the specific conserved regions of DENV I/II/III/IV. (ultrassay.com)
The detection of DENV-3 in years when no human autochthonous cases of this serotype were recorded suggests that silent circulation of DENV-3 is occurring and indicates that green areas may be maintaining serotypes that are not circulating in the human population, possibly by a vertical transmission mechanism. (nature.com)

that permitted rapid, sensitive and economical detection for this pathogen was successfully developed. (omicsdi.org)

A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. (butantan.gov.br)
The limit of detection was 10-100 DNA copies per reaction. (tbenews.com)

In this study, we first described a loop-mediated isothermal amplification (LAMP) method for the rapid detection of capsular polysaccharide synthesis regulating gene rcsA from K. pneumoniaein clinical samples by using two methods including real-time turbidity monitoring and fluorescence detection to assess the reaction. (omicsdi.org)
After all, we decided to modify his method by substituting luciferase for fluorescence protein and designed a new detection method. (igem.org)

33 Antigen detection assays are not available for MVEV, but have been used for JEV and Dengue fever virus. (mja.com.au)

In addition, Excivion has a pending patent (in the US and elsewhere) on the novel lateral-flow test architecture for flaviviruses generally, which features a number of innovations over and above the use of hyperglycosylated antigens. (prfire.co.uk)

Data on the detection of Zika virus RNA in semen can inform estimates of the periods during which sexual transmission might occur. (cdc.gov)

It has become of particular relevance in Germany in 2019 due to its rapid geographical spread and the detection of the first human clinical cases. (mdpi.com)

Kunjin virus was the only other flavivirus tested that produced a band of the appropriate size. (usuhs.edu)

Arenavirus , Filoviridae , Bunyaviridae and Flavivirus . (who.int)

RT-PCR assays for detection of specific genomic sequence of DEV have shown high sensitivity and specificity. (biomedcentral.com)
These technology breakthroughs can revolutionize point-of-care rapid assays for detection of acute infections including Zika. (prnewswire.com)

The objective of this study is to develop a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of the new virus related to Tembusu-related Flavivirus. (biomedcentral.com)

This work reports the initial characterization, including near full-length sequence analysis, of an ISF which seems to form a divergent lineage within the ISF radiation, as indicated by phylogenetic analysis based on ac multiple sequence alignment of the NS5 flavivirus coding sequence. (unl.pt)
RT-qPCR and nested qPCR for detection and characterization of this virus as well as a methodology for subtyping were developed and the first detected case of subtype 3b in a German wild animal was documented. (uni-greifswald.de)

In addition, the RT-LAMP assay for newly isolated Tembusu-related Flavivirus showed higher sensitivity with an RNA detection-limit of 2 copies/μL compared with 190 copies/μL of the conventional RT-PCR method. (biomedcentral.com)
However, we were disappointed with the sensitivity of existing test architectures and, felt compelled to invent a more sensitive test architecture befitting the sensitivity needs of detection of historic dengue. (prfire.co.uk)
The sensitivity of the detection system for bla KPC-producing Serratia spp. (omicsdi.org)
The results showed that the detection limit of the LAMP method was 0.115 pg/μl DNA within 60 min under isothermal conditions (61°C), a 100-fold increase in sensitivity compared with conventional PCR. (omicsdi.org)

As a Virologist she's conducted doctoral and postdoctoral research on arboviruses including flaviviruses. (cdc.gov)
NAMAC assists in the detection, management and control of real or potential outbreaks of arboviruses or malaria and provides advice on the risk of these diseases or exotic vectors being imported from overseas. (health.gov.au)
Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. (butantan.gov.br)

Gary Dubin explains: "Application of a well-established platform, the inactivated virus approach used for other licensed Flavivirus vaccines (such as tick borne encephalitis virus and Japanese encephalitis virus vaccines), gave us a higher probability of technical success in addressing the problem urgently. (ifpma.org)

All 30 non- K. pneumoniae strains tested were negative for LAMP detection, indicating the high specificity of the LAMP reaction. (omicsdi.org)

Antibody detection by indirect immunofluo- rhages, gum bleeding, epistaxis, menorrhagia, and gastroin- rescence has proved to be at least as reliable as hemagglutina- testinal hemorrhage, occur. (cdc.gov)
It can be inferred from our study that for detection of dengue in the early febrile period (1-5 days), estimation of dengue-specific serum IgM is the most sensitive antibody detection method. (ijmedicine.com)

Serological results should be interpreted according to previous exposure to other flaviviral infections and the flavivirus vaccination status. (europa.eu)

The desired amplification was achieved using selected primers and detection was possible using both the calcein indicator method and the real-time turbity recording system at 65?C for 60 min. (omicsdi.org)
Detection and identification of Toscana and other phleboviruses by RT-nested-PCR assays with degenerated primers[J]. J Med Virol, 2003,71(1):140-149. (bmsw.net.cn)
Generic RT-nested-PCR for detection of flaviviruses using degenerated primers and internal control followed by sequencing for specific identification[J]. J Virol Methods,2005,126(1/2):101-109. (bmsw.net.cn)

En Paraguay los virus dengue, fiebre amarilla y Zika fueron detectados en infecciones humanas, pero los estudios de flavivirus en mosquitos son aún escasos. (bvsalud.org)
These results bring new information about enzootic activity of flaviviruses in Paraguay. (bvsalud.org)

This paper discusses the factors contributing to the rising burden and the challenges facing early detection, laboratory confirmation and rapid response to outbreaks and proposes strategic directions for prevention and control. (who.int)
Because of this uncertainty, early detection of severe dengue and proper case management is essential. (knowdengue.com)

The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)

The RT-LAMP assay for newly isolated Tembusu-related Flavivirus is a valuable tool for the rapid and real-time detection not only in well-equipped laboratories but also in general conditions. (biomedcentral.com)
RICHMOND, BC , Aug. 19, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid infectious disease tests, is pleased to announce that it has filed two patent applications with the U.S. Patent and Trademark Office relating to multiple color rapid detection assay systems. (prnewswire.com)
In the present study, a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Serratia spp. (omicsdi.org)
Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China. (omicsdi.org)
A rapid and sensitive molecular method for the detection of K. pneumoniae in clinical samples is needed to guide therapeutic treatment. (omicsdi.org)
In conclusion, we have developed a rapid and sensitive visual K. pneumoniae detection LAMP assay, which could be a useful tool for clinical screening, on-site diagnosis and primary quarantine purposes. (omicsdi.org)
Sensitive and rapid detection of the new Delhi metallo-beta-lactamase gene by loop-mediated isothermal amplification. (omicsdi.org)
We describe a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of bla(NDM-1) from pure culture and sputum, urine, and fecal samples. (omicsdi.org)
A general and relatively rapid method is the direct detection and examination of the agent's genome. (uni-greifswald.de)

Based on his experience, he taught us about all about flavivirus - genetic and phenotypic features, potential risks, obstacles that current clinical methods have. (igem.org)

It is a member of the flavivirus family (group B arbovirus). (medscape.com)

Detection of yellow fever virus genomes from four imported cases in China. (cdc.gov)

Among the most potent antagonists is non-structural protein 5 (NS5), which has been shown for all disease-causing flaviviruses to target different steps and players of the type I IFN signaling pathway. (shengsci.com)

The report also includes data on the sentinel chicken surveillance, exotic mosquito detections at the border and other mosquito surveillance activities. (health.gov.au)
1 Awareness, early engagement and collaboration with healthcare professionals can improve detection, which can translate to prompt dengue control and surveillance in the community. (knowdengue.com)

Two new RT-qPCR for the detection of both lineages were developed, and reportedly used by independent laboratories. (uni-greifswald.de)

In 2011-12, sentinel chicken programs for the detection of flavivirus activity were conducted in most states with the risk of arboviral transmission. (health.gov.au)
The present study showed that goats are useful sentinel animals for the detection of new TBEV risk areas. (biomedcentral.com)

Anatomy3

Organisms20

Diseases12

Chemicals and Drugs15

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes10

Disciplines and Occupations1

Information Science4

Health Care4

Antibodies16

Genus7

Mosquitoes12

Mosquito-borne6

Flaviviridae1

Dengue virus4

Viruses11

ZIKV13

Positive for flavivirus1

Cross-reactivity2

Closely related2

Viral5

Pathogens2

Aedes1

DENV3

Pathogen1

Limit of detect2

Fluorescence2

Antigen detection1

Antigens1

Zika Virus1

20191

Kunjin1

Arenavirus1

Assays for detection2

Assay for the detection1

Characterization2

Sensitivity4

Arboviruses3

Vaccines1

Specificity1

Antibody detection2

Infections1

Primers3

Paraguay2

Early detection2

Virus detection1

Rapid9

Clinical1

Family1

Genomes1

Protein1

Surveillance2

Lineages1

Sentinel2