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ChromatographyChromatography, High Pressure LiquidChromatography, Thin LayerCoccidiostatsFermentationHot FlashesStreptomycesChromatography, AffinityMagnetic Resonance SpectroscopyPhysicochemical PhenomenaChemistry, PhysicalChromatography, GelChromatography, Ion ExchangeMolecular StructureChromatography, GasChromatography, LiquidMolecular WeightCountercurrent DistributionMicrobial Sensitivity TestsElectrophoresis, Polyacrylamide GelChromatography, DEAE-CelluloseKineticsMass SpectrometryMolecular Sequence DataAmino Acid SequenceIsoelectric FocusingAmino AcidsChromatography, AgaroseGas Chromatography-Mass SpectrometryHydrogen-Ion ConcentrationChemistrySpectrophotometry, UltravioletChromatography, Reverse-PhaseChemical PhenomenaSolubilityChromatography, PaperCattleChemical FractionationSubstrate SpecificityMethodsIsoelectric PointEscherichia coliSepharoseCarbohydratesPhotolysisSolventsSpectrometry, Mass, Electrospray IonizationTemperatureRabbitsLiverImmunodiffusionProtein BindingIndicators and ReagentsTandem Mass SpectrometryCarbohydrate SequenceDrug StabilityPeptide FragmentsElectrophoresisUltracentrifugationMacromolecular SubstancesHydrolysisPlant ExtractsOctanolsOligosaccharidesStereoisomerismBase SequenceTrypsinBacterial ProteinsWaterBinding SitesPeptidesRecombinant ProteinsReproducibility of ResultsTime FactorsGlycoproteinsElectrophoresis, DiscSpectrophotometryLightCalibrationCell MembraneChromatography, Micellar Electrokinetic CapillaryBusulfanPhospholipidsChemical PrecipitationGlycosidesMethanolHot TemperatureCloning, MolecularSwineOxidation-ReductionDetergentsGlucosidesSpecies SpecificityFatty AcidsProteinsBiological Assay1-OctanolSpectrometry, Mass, Fast Atom BombardmentCentrifugation, Density GradientAmmonium Sulfate
Centrifugal Partition ChromatographySupercritical Fluid ChromatoCompoundsPurificationAnalyticalLiquidSeparationsStationary phaseSoluteSolventAnalyzeProteinsMoleculesPrepColumnsDetectorPrincipleSensitivitySystemColumnTechniquesAnalysisTechniqueReversed-phaseOrganicSystemsScientificCurrentOperationExchange
Centrifugal Partition Chromatography3
Supercritical Fluid Chromato2
Compounds8
  • With the capability to purify compounds by preparative HPLC and flash chromatography on the same instrument, Gilson's PLC 2050/2250/2500 Purification Systems simplify and streamline compound purification. (thomassci.com)
  • Chromatography allows for the separation of a mixture of compounds by diluting the mixture into a mobile phase which then passes through or over a stationary phase to create separation by the interaction and affinity of individual compounds for one of the phases of the chromatography system ( Figure 1a ). (cannabissciencetech.com)
  • The basic function of chromatography procedures is to separate a mixture of components into their constituent compounds. (cannabissciencetech.com)
  • Preparative chromatography is used to separate compounds for later isolation or purification which then can be used in other processes. (cannabissciencetech.com)
  • Gas chromatography (GC) uses a carrier gas, such as hydrogen or helium, to separate compounds based on their interaction with a stationary phase or based on their boiling points. (cannabissciencetech.com)
  • In liquid chromatography (LC), compounds are separated by dissolving a sample into a liquid mobile phase which is then passed over a solid stationary phase. (cannabissciencetech.com)
  • A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
Purification3
  • Polar pharmaceuticals are notoriously difficult to separate by analytical and semi-prep HPLC and this research demonstrates how we have generated reproducible separations of polar pharmaceuticals using low-pressure flash purification in 'HILIC' mode. (chromatographytoday.com)
  • These separations are not trivial by HPLC, and often reversed-phase purification is the 'go-to' method but can be challenging. (chromatographytoday.com)
  • One recent article describes the use of hydrophilic solid phase extraction of glycyrrhizin (GA) in liquorice coupled with reversed-phase liquid chromatography purification, and although this method improved enrichment of GA, HILIC was only employed as a crude extraction procedure [9]. (chromatographytoday.com)
Analytical8
Liquid13
Separations1
  • We demonstrate the feasibility using standard silica flash columns to open up new possibilities for amino acid, nucleoside and nucleotide separations. (chromatographytoday.com)
Stationary phase4
  • It is thought analytes partition into the water-rich layer that is partially immobilised onto the stationary phase, and elute with increasing polar solvent [1]. (chromatographytoday.com)
  • The properties of the mobile phase and the stationary phase are opposite of each other and allow for the partitioning of analytes as the mobile phase and test mixture flow through or over the stationary phase ( Figure 1b ). (cannabissciencetech.com)
  • Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
Solute1
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Solvent2
Analyze1
Proteins1
  • The researchers concluded that HPLC analysis can be approached via a multi-user instrument or applied in high throughput workflows for evaluating and analyzing membrane proteins. (readnewshourly.com)
Molecules1
  • This characteristic has made HPLC lab employ this technique to assess the purity and content analysis of pharmaceutical molecules. (readnewshourly.com)
Prep1
  • Fitting to your needs and environment The Pure chromatography instruments are extremly compact, ensure safety on the highest level and are easy to use for any flash or prep HPLC application. (thomassci.com)
Columns1
Detector1
  • The dry air supply supplies particle free dry air on demand for the evaporative light scattering detector (ELSD) system of a Chromatography system. (thomassci.com)
Principle1
Sensitivity1
System3
  • The operation of the dry air supply is controlled by the Chromatography system software. (thomassci.com)
  • Used Gilson Preparative HPLC and Flash Chromatography System. (bidspotter.com)
  • This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time. (selectscience.net)
Column1
Techniques1
Analysis4
Technique2
  • HPLC Lab Secrets: How This Revolutionary Technique Will Change Your Scientific World! (readnewshourly.com)
  • They concluded that HPLC is a specific, sensitive, rapid, and accurate technique for routine clinical investigations. (readnewshourly.com)
Reversed-phase1
Organic1
  • The aqueous layer was extracted twice more with DCM, the organic layers combined, dried with MgSO4, filtered through cotton, concentrated and purified by automated chromatography with a 0percent to 3percent gradient of EtOAc in hexanes to yield 1.24 g (78percent). (ambeed.com)
Systems2
  • In clinical sciences and drug development, HPLC systems are used widely to quantitate antibiotics. (readnewshourly.com)
  • HPLC systems have been a defining tool for the last four decades, and their importance and applications will likely increase. (readnewshourly.com)
Scientific1
  • The current article focuses on understanding HPLC lab s and their impact on the scientific world. (readnewshourly.com)
Current1
  • current HPLC minutes are approved used to state specimens, trailing HDL, but do ContributionsConceived Published by personal read Radicalism and of the setpoints. (democo.de)
Operation1
Exchange1