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  • nitrogen
  • The dynamics of the nitrogen fixation activity of the root nodules, the growth of the vegetative mass of plants and the change in the activity of antioxidant enzymes (superoxide dismutase, ascorbate and guaiacol peroxidase) in different soybean organs for treatment of seeds by rhizobia incubated with lectin, in combination with fungicides have been studied. (dp.ua)
  • It was found that processing of soybean seeds with fungicides (Fever and Maxim XL) together with rhizobium inoculation contributed to the preservation of the nitrogen fixation activity of the root nodules and the growth of vegetative mass of plants. (dp.ua)
  • At the same time, the inhibition of the growth of vegetative mass of plants and their symbiotic properties occurred, as evidenced by the decrease in the nitrogen fixation activity of the root nodules for the joint treatment of seeds with fungicides and lectin. (dp.ua)
  • Response of Glycine max in relation to nitrogen fixation as influenced by fungicide seed treatment. (dp.ua)
  • Symbiotic nitrogen (N) fixation is the most important N input to many ecosystems. (pubmedcentralcanada.ca)
  • Nitrogen fixation (we hereafter drop the term 'symbiotic' since all N fixation we discuss here is symbiotic) is also at the heart of one of the most intriguing patterns in ecosystem ecology. (pubmedcentralcanada.ca)
  • They are aerobic, free-living soil microbes which play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen , which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil ( nitrogen fixation ). (wikipedia.org)
  • We explore quantitative assessments of these strategies to study the prokaryotic trait of nitrogen fixation, the enzyme-catalyzed reduction of N 2 to ammonia. (pnas.org)
  • Phylogenies constructed on nitrogen fixation genes are not in agreement with the tree-of-life based on 16S rRNA but do not conclusively distinguish between gene loss and LGT hypotheses. (pnas.org)
  • We try to address these weaknesses by exploring the use of quantitative measures for LGT and we apply our methods to the trait of nitrogen fixation (NIF) ‖ as a case study. (pnas.org)
  • All organisms depend on utilizable nitrogen, but only a few prokaryotes can obtain it from atmospheric nitrogen (N 2 ) through conversion of N 2 to ammonia, catalyzed by the heterodimeric enzyme nitrogenase. (pnas.org)
  • Rubisco
  • Our research focuses on the development and use of synthetic biology tools to scrutinize the biology of the biospheres most abundant protein, the photosynthetic CO 2 -fixing enzyme Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). (edu.au)
  • Sharwood, R, Ghannoum, O & Whitney, S.M (2016) 'Prospects for improving CO 2 fixation in C 3 -crops through understanding C 4 -Rubisco biogenesis and catalytic diversity', Current Opinion in Plant Biology , vol. 31, pp. 135-142. (edu.au)
  • Whitney S.M ., Houtz R.L and Alonso H (2011) Advancing our understanding and capacity to engineer nature's CO 2 sequestering enzyme, Rubisco. (edu.au)
  • Wilson, R & Whitney, S.M (2017) 'Improving CO 2 Fixation by Enhancing Rubisco Performance', in Miguel Alcalde (ed. (edu.au)
  • Two different types of carboxysomes have been identified (α- and β-carboxysomes), according to the types of the CO 2 -fixing enzyme, Rubisco (form 1A and form 1B), possessed in cyanobacteria. (plantphysiol.org)
  • Through these mechanisms, carboxysomes elevate the CO 2 concentration in the vicinity of Rubisco and thereby enhance the efficiency of carbon fixation. (plantphysiol.org)
  • Enhancement and reduction in Rubisco (photosynthetic CO2 fixation enzyme) content by transformation with a sense or an antisense RBCS construct had an advantage for growth at low and elevated [COlevels, respectively. (nii.ac.jp)
  • Four gene clusters (termed cbb1-4 ) in the A. ferrooxidans genome are predicted to encode enzymes and structural proteins involved in carbon assimilation via the Calvin-Benson-Bassham (CBB) cycle including form I of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO, EC 4.1.1.39) and the CO 2 -concentrating carboxysomes. (biomedcentral.com)
  • Proteins
  • Proteins, particularly enzymes, are rendered insoluble in aqueous media by chemical activation of a polymeric support and crosslinking of the active proteins and the support by an inter-molecular bridging agent. (google.com)
  • This invention relates to the insolubilisation of proteins, more particularly but not exclusively of enzymes. (google.com)
  • Molecular analyses of V-H + PPase pumps, which acidify the acidocalcisome lumen and are diagnostic proteins of the organelle, also reveal the presence of this enzyme in all three superkingdoms suggesting it is ancient and universal. (biomedcentral.com)
  • Some of the proteins in the capsid are enzymes. (angelfire.com)
  • symbiotic
  • The degree of reaction of antioxidant enzymes in the studied symbiotic systems Glycine max - Bradyrhizobium japonicum depends on the nature of the active substance fungicides and the manifestation of their joint effect in a complex with rhizobia incubated with lectin. (dp.ua)
  • vitro
  • In vitro scaffolding of enzymes can be achieved by, i.e., cross-linking, encapsulation, and binding to nucleic acid or protein scaffolds. (frontiersin.org)
  • Besides designing covalent/irreversible or reversible synthetic protein complexes for metabolic engineering, three-dimensional (3-D) printing of enzyme arrays may enable the design of in vitro protein channels. (frontiersin.org)
  • organization
  • Within the review article, we will first discuss the supramolecular organization of enzymes in living systems and second summarize current and future approaches to design artificial metabolic channels by additive manufacturing for the efficient production of desired products. (frontiersin.org)
  • suggests
  • The universal distribution of the V-H + PPase PF03030 domain, which is associated with the V-H + PPase function, suggests the domain and the enzyme were already present in the Last Universal Common Ancestor (LUCA). (biomedcentral.com)
  • This suggests a novel regulatory connection between CO 2 fixation and tryptophan biosynthesis. (biomedcentral.com)
  • synthetic
  • Application of industrial enzymes for production of valuable chemical compounds has greatly benefited from recent developments in Systems and Synthetic Biology. (frontiersin.org)
  • To circumvent these problems, covalent linkages between the synthetic scaffold platform and the enzymes to be arranged would be advantageous. (frontiersin.org)
  • protein
  • By dissecting the mechanism of spontaneous intramolecular isopeptide formation within the CnaB2 domain of the fibronectin-binding protein FbaB from Streptococcus Pyogenes (Spy), Howarth and co-workers developed a versatile tool to allow covalent binding of tagged-enzymes to modified macromolecules ( Zakeri and Howarth, 2010 ). (frontiersin.org)
  • form
  • Enzymes to be bound are usually handled in the form of buffered dilute aqueous solutions. (google.com)
  • activity
  • Under these conditions, the intensification of the activity of superoxide dismutase and ascorbate peroxidase was observed, as well as inhibition of the activity of guaiacol peroxidase in soybean root nodules in the phase of three true leaves and increased activity of all investigated enzymes in the phase of mass flowering. (dp.ua)
  • We demonstrated that β-carboxysome biosynthesis is accelerated in response to increasing light intensity, thereby enhancing the carbon fixation activity of the cell. (plantphysiol.org)
  • 2. The activity of immobilized enzymes amounts to 100-10,000 U/g. (springer.com)
  • support
  • Firstly, insolubilisation can be achieved by physical adsorption of the enzyme on an inert support or on ion exchanger resins. (google.com)