• Catalysis of the reaction: O2 + ATP + Photinus luciferin = light + pyrophosphate + AMP + CO2 + oxidized Photinus luciferin. (yeastrc.org)
  • Synchronous fireflies (Photinus carolinus) are one of at least 19 species of fireflies that live in Great Smoky Mountains National Park. (nps.gov)
  • Let's take a look at a very common firefly, Photinus pyralis (sorry - they don't have common names). (adirondackalmanack.com)
  • Fireflies, or lightning bugs according to the neighbour who is a great nature lover says they can be found all over the eastern parts of North America, but the Great Smoky Mountains is one destination that attracts thousands of visitors year after year to see the Elkmont fireflies Synchronous fireflies (Photinus carolinus). (bellaonline.com)
  • Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola . (thermofisher.com)
  • Luciferase proteins from Photinus (firefly), Renilla and Gaussia were also characterized and tested as reporters. (plos.org)
  • Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS. (umassmed.edu)
  • Together with other luminous beetles (Rhagophthalmidae, Phengodidae and some Elateridae) in the same superfamily Elateroidea 1 , fireflies can produce light in the peroxisome of photocytes within the luminous organs with diverse morphology and location 3 by a common oxidative mechanism of luciferin catalyzed by luciferases in the presence of ATP, O 2 and Mg 2+ . (nature.com)
  • Liz Langley, National Geographic , "How bioluminescence works in nature," 2 May 2019 Curiously, there are far fewer luciferins than luciferases. (merriam-webster.com)
  • Quanta Magazine , "In the Deep, Clues to How Life Makes Light," 1 Dec. 2016 While species tend to have unique luciferases, many share the same luciferin . (merriam-webster.com)
  • Would VF luciferin work with other luciferases? (igem.org)
  • We've now shown that yellow-green luciferases have a smaller cavity at the active site where luciferin binds and is oxidized to oxyluciferin," Viviani said. (eurekalert.org)
  • Luciferases from click beetles, fireflies, and railway worms catalyze the ATP-dependent decarboxylation of luciferin (Figure 1). (encyclopedia.com)
  • however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate. (fishersci.ca)
  • Luciferases, Firefly" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Luciferases, Firefly" by people in this website by year, and whether "Luciferases, Firefly" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Luciferases, Firefly" by people in Profiles. (umassmed.edu)
  • The light is produced inside the tiny dinoflagellates body thanks to two chemicals: the enzyme luciferase and the compound luciferin . (merriam-webster.com)
  • Quanta Magazine , "In the Deep, Clues to How Life Makes Light," 1 Dec. 2016 Like many bioluminescent animals their light is created by a reaction between the light-emitting molecule luciferin and the enzyme luciferase. (merriam-webster.com)
  • In general, a bioluminescent reaction requires a substrate (generically called the luciferin), an enzyme (luciferase), and the presence of molecular oxygen with or without cofactors ( 3 , 4 ). (sciencemag.org)
  • Bioluminescence imaging (BLI) exploits the remarkable properties of a firefly enzyme (Luciferase) to emit light whilst catalysing a substrate (Luciferin) to optically image biological events in living animals. (ucl.ac.uk)
  • Inside the light-producing organ of fireflies are the enzyme luciferase and the pigment luciferin. (reference.com)
  • Fireflies combine the chemical luciferin and oxygen with the enzyme luciferase in their lanterns (part of their abdomens) to make light. (nps.gov)
  • Fireflies produce light through a chemical reaction between luciferin and it's counterpart, the enzyme luciferase. (innovationtoronto.com)
  • He named the substrate "luciferin" and the enzyme "luciferase" (Simon 116). (ukessays.com)
  • Scientists at Syracuse University also used nanoscience to produce light biologically by looking to the firefly, which produces light when luciferin interacts with the enzyme luciferase. (ecmag.com)
  • It produces a blue-coloured light by a specialized chemical reaction of the substrate luciferin and the enzyme luciferase. (wikipedia.org)
  • Much of the early work on the chemistry of the firefly luminescence was done in the lab of William D. McElroy at Johns Hopkins University. (wikipedia.org)
  • In particular, the invention relates to a heterocycle compound which produces a luminescence at a light wavelength different from that of firefly luciferin in nature. (patentgenius.com)
  • It is known that this bioluminescence of the firefly emit a luminescence by chemically reacting with the luciferin which is luminescent substrate through the carton of the luciferase which is luminescent enzyme. (patentgenius.com)
  • Oxidation of firefly luciferin results in luminescence. (harvard.edu)
  • Pyrophosphate and tripolyphosphate affect firefly luciferase luminescence because they act as substrates and not as allosteric effectors. (harvard.edu)
  • Firefly luminescence: a historical perspective and recent developments. (harvard.edu)
  • Lemasters JJ, Hackenbrock CR (1977) Kinetics of product inhibition during firefly luciferase luminescence. (springer.com)
  • In one of them, the comparative genomics of two fireflies and one luminous click beetle support parallel origin of bioluminescence in beetles 13 . (nature.com)
  • Fireflies (also called lightning bugs) are beetles. (nps.gov)
  • Fireflies are beetles which take from one to two years to mature from larvae, but will live as adults for only about 21 days. (bellaonline.com)
  • There are more than 2,000 species of fireflies, or lightning bugs, and they are actually winged beetles, not flies. (livescience.com)
  • Fireflies aren't flies - they are beetles, as you can tell from the hard shell that protects their wings when they're not flying. (japantimes.co.jp)
  • Firefly luciferin and modified substrates are fatty acid mimics and have been used to localize fatty acid amide hydrolase (FAAH) in vivo. (wikipedia.org)
  • Moreover, our results support the conjecture that, in the bioluminescence reaction, the fraction of LH2-AMP that is oxidized into L-AMP, relative to other inhibitory products or intermediates, increases when the concentrations of the substrates ATP and luciferin increases. (nih.gov)
  • The bioluminescence produced by luciferase, a firefly enzyme, requires three substrates: luciferin, ATP and oxygen. (springer.com)
  • They have also used synthetic organic techniques to prepare novel substrates for the firefly protein (luciferin) and substrates for a bioluminescent jellyfish that emits blue and green light. (conncoll.edu)
  • Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin. (umassmed.edu)
  • Synthesis of luciferyl coenzyme A: a bioluminescent substrate for firefly luciferase in the presence of AMP. (harvard.edu)
  • D-Luciferin, Free Acid is a luminescent substrate for firefly luciferase. (agscientific.com)
  • A dual or triple fusion reporter gene construct harboring various fluorescent proteins (i.e., enhanced green fluorescent protein ( egfp ), red fluorescent protein ( rfp ), and different luciferase genes (i.e., firefly luciferase ( fl ), renilla luciferase ( rl ), and herpes simplex virus type 1 thymidine kinase ( tk ) gene)) can combine the advantage of fluorescence, bioluminescence, and radionuclide imaging techniques and overcome the shortcomings of each imaging modality [ 6 ]. (hindawi.com)
  • This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. (biotium.com)
  • Bioluminescence identified from firefly beetle is an example of ATP‐dependent photo‐production, whereas in jellyfish and Renilla sp. (els.net)
  • Masahiro Kiyama, Ryohei Saito, Satoshi Iwano, Rika Obata, Haruki Niwa and Shojiro A. Maki, "Multicolor Bioluminescence Obtained Using Firefly Luciferin", Current Topics in Medicinal Chemistry (2016) 16: 2648. (eurekaselect.com)
  • The in vivo synthesis of firefly luciferin is not completely understood. (wikipedia.org)
  • In preclinical cancer research, bioluminescence imaging with firefly luciferase and D -luciferin has become a standard to monitor biological processes both in vitro and in vivo . (nature.com)
  • In recent times, cyclic alkylaminoluciferin (CycLuc1) ( λ max =604 nm) demonstrated improved detection of targets in vivo by a significantly increased bioluminescence production over D -luciferin 9 . (nature.com)
  • However, these attempts have failed to show a significant improvement in the detection of targets in vivo when compared with D -luciferin, owing to their low bioluminescence production. (nature.com)
  • Tested and Certified for in vivo imaging Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. (thomassci.com)
  • Journal Article] Rational Design and Development of Near-infrared-emitting Firefly Luciferins Available in vivo. (nii.ac.jp)
  • Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. (goldbio.com)
  • This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg 2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain. (goldbio.com)
  • Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. (goldbio.com)
  • RediJect D-Luciferin (K+ salt) is a bioluminescent in vivo substrate in a ready-to-use pre-formulated injectable format developed by the in vivo imaging leaders to fit your laboratory workflow. (perkinelmer.com)
  • Luciferin Amides Enable in Vivo Bioluminescence Detection of Endogenous Fatty Acid Amide Hydrolase Activity. (umassmed.edu)
  • For example, the adenosine-5′-triphosphate (ATP)-dependent enzymatic firefly bioluminescence reaction has long been utilized as a microbial monitoring tool. (eurekaselect.com)
  • The effect of CoA on the characteristic light decay of the firefly luciferase catalysed bioluminescence reaction was studied. (nih.gov)
  • 10. The influenza viral neuraminidase detection reagent of claim 9 which further comprises one or more chemicals for firefly luciferase-catalyzed biochemiluminescence reaction except for firefly luciferin. (freepatentsonline.com)
  • 13. The method according to claim 12, wherein said released firefly luciferin is detected via a biochemiluminescence reaction in the presence of firefly luciferase. (freepatentsonline.com)
  • Fireflies glow due to a chemical reaction in a specialized organ within their body. (reference.com)
  • Luciferase and luciferin combine with calcium and the high-energy molecule adenosine triphosphate to begin the reaction. (reference.com)
  • Oxygen binds to a chemical called a luciferin and undergoes a chemical reaction, helped along by an enzyme called luciferase. (bbc.com)
  • Although all bioluminescent creatures use much the same reaction, the exact nature and structure of the luciferin and luciferase vary dramatically across different species. (bbc.com)
  • Luckily for the survival of fireflies, the chemical reaction doesn't produce heat or else they'd incinerate themselves every time they glowed. (blueridgeoutdoors.com)
  • Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. (fishersci.ca)
  • The second major element of an ATP sanitation monitoring system is the use of a luminometer to measure the light produced by the reaction of ATP with luciferin and luciferase. (neogen.com)
  • Since the amount of luciferin and luciferase are fixed, the amount of light that is produced by the chemiluminescent reaction is directly proportional to the amount of ATP collected. (neogen.com)
  • The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. (abcam.com)
  • The mechanism of light emission is broadly conserved: an enzymatic oxidation reaction by a luciferase enzyme turns a luciferin substrate into a high energy intermediate, which decays to produce light ( Shimomura, 2006 ). (elifesciences.org)
  • Synchronous fireflies produce light in their lanterns, the pale area of the abdomen visible on the underside of the insect above. (nps.gov)
  • Peak flashing for synchronous fireflies in the Smoky Mountains park is normally within a two-week period in late May to mid-June. (bellaonline.com)
  • Peak flashing for synchronous fireflies in the park is normally within a two-week period in mid-June. (yourcabin.com)
  • both use a naturally occurring molecule called luciferin , named for Lucifer, the light-bearer. (merriam-webster.com)
  • This protein produces a dim light when it comes in contact with another molecule called luciferin. (brightsurf.com)
  • For example, an enzyme known as luciferase in fireflies produces light by acting on a molecule called luciferin. (elifesciences.org)
  • Luciferase is an enzyme that catalyzes the oxidation of luciferin in fireflies, producing oxyluciferin and enabling fireflies to emit light. (eurekalert.org)
  • Some firefly eggs emit a faint glow when disturbed leading scientists to believe that fireflies produce light to warn predators away. (blueridgeoutdoors.com)
  • When luciferin reaches cells that have been altered to carry the firefly gene, those cells emit light. (photonics.com)
  • Other insects may dance, chirp, emit pheromones or show bright colors to attract mates, but fireflies are one of the few insects that shine a light. (japantimes.co.jp)
  • Firefly luciferin (also known as beetle luciferin) is the luciferin, or light-emitting compound, used for the firefly (Lampyridae), railroad worm (Phengodidae), starworm (Rhagophthalmidae), and click-beetle (Pyrophorini) bioluminescent systems. (wikipedia.org)
  • The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder, D-Luciferin. (antibody-antibodies.com)
  • Firefly Luciferin is identical to Beetle Luciferin. (goldbio.com)
  • Fireflies (Lampyridae) are one of the best-known luminescent organisms since the time of Aristotle 1 , and thus an important subject of scientific studies, especially related to their bioluminescent behavior and biochemistry. (nature.com)
  • D-Luciferin is commonly used as a substrate for imaging the expression of the luc marker gene as well as luciferase fusion conjugates in living cells, tissues and organisms. (p212121.com)
  • Bioluminescent organisms such as fireflies have luciferin-luciferase system that enables bioluminescence. (biology-online.org)
  • This excretion contains luciferin, the same light-emitting biological pigment found in fireflies, although the worm's slime glows continuously. (livescience.com)
  • ATP bioluminescence occurs when ATP from a sample comes into contact with luciferase, an enzyme found in fireflies, and luciferin, a substrate. (neogen.com)
  • Firefly luciferin is the molecule responsible for the firefly's glow. (shapeways.com)
  • How do fireflies glow? (reference.com)
  • The glow of a firefly is known as bioluminescence, and is a cold light, meaning it does not produce heat. (reference.com)
  • The ability of the firefly to regulate its glow and produce a specific pattern of flashes is still a mystery to researchers. (reference.com)
  • Fireflies are found in all U.S. States, but not all of the species glow, according to Smithsonian Magazine. (reference.com)
  • The US government's National Institute of Environmental Health Services (NIEHS) has been funding research into fish that glow like a firefly when exposed to polluted water, a patent application reveals. (newscientist.com)
  • The cancer cells were then incubated with Luciferin, the substance that makes fireflies glow. (naturalnews.com)
  • The glow of the firefly is a natural light called biolumenesence. (adirondackalmanack.com)
  • So why do fireflies glow? (blueridgeoutdoors.com)
  • Fireflies glow well before they are ready to mate. (blueridgeoutdoors.com)
  • The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications. (thermofisher.com)
  • This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme. (thermofisher.com)
  • Glow worms are actually just fireflies ( Lampyridae ) in their larvae stage. (livescience.com)
  • Yes, fireflies glow even when they are just tiny, wingless larvae, radiating a single, unwavering green light on their foreheads. (livescience.com)
  • Even the eggs glow, because they too contain luciferin. (japantimes.co.jp)
  • Philips' bio-light, consisting of glass jars filled with bioluminescent bacteria enhanced with firefly genes, emitted a greenish glow when fed methane gas. (ecmag.com)
  • The final ingredient is oxygen, which the firefly acquires through small openings along its abdomen. (adirondackalmanack.com)
  • Luciferase/luciferin Oxygen ATP. (slideserve.com)
  • By controlling the supply of oxygen to the luciferin, the insect can turn the light on and off, and so produce a flashing signal. (japantimes.co.jp)
  • In a typical assay, microbial cells can be treated so as to release their ATP into solution where it can react with bioluminescent assay reagents, such as firefly luciferase enzyme and luciferin substrate, in the presence of magnesium and oxygen, to produce photons. (google.com.au)
  • Additional relatives include the nontechnical adjective luciferous, meaning "bringing light or insight," and luciferase, the enzyme that catalyzes the oxidation of luciferin. (merriam-webster.com)
  • The oxidation of luciferin through the enzymatic activity of luciferase results in the emission of light (or photon). (biology-online.org)
  • When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity. (thermofisher.com)
  • The science behind the instantly accurate systems stems from the study of fireflies and adenosine triphosphate (ATP) - a molecule that exists in every living cell. (neogen.com)
  • Combining gene expression analyses with comparative genomics, we propose a more complete luciferin synthesis pathway, and confirm the convergent evolution of bioluminescence in insects. (nature.com)
  • Useful in automated peptide synthesis for incorporation of amino-luciferin into synthetic peptides. (antibody-antibodies.com)
  • Synthesis and Emission of Light Activity of the Firefly Luciferin Analog (2)," the Chemical Society of Japan, the 87th spring annual convention, Mar. 12, 2007, p. 1245, 4 H4-31. (patentgenius.com)
  • These reagents and methods disclosed in the present invention enable simple, rapid, specific and sensitive detection of influenza virus types A and B. These reagents are N-acetylneuraminic acid-firefly luciferin conjugates which can be cleaved by influenza virus neuraminidase. (freepatentsonline.com)
  • The Thermo Scientific™ Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly. (fishersci.ca)
  • Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. (fishersci.ca)
  • Rapid and sensitive firefly luciferin-luciferase combinations are used not only to measure cell viability but also for reporter-gene assays. (eurekaselect.com)
  • Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications. (harvard.edu)
  • The firefly luciferase (fl) , monomeric red fluorescence protein (mrfp) , and truncated herpes simplex virus type 1 thymidine kinase SR39 mutant (ttksr39) were fused together to create TF reporter gene constructs with different order. (hindawi.com)
  • Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors. (thermofisher.com)
  • Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. (biotium.com)
  • Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays. (goldbio.com)
  • Also, we report that luciferin, the substrate for the reporter luciferase ( LUC ) gene, is able to activate expression of PR genes, and this may pose a problem when using this gene reporter system in studies related to plant defense. (plantphysiol.org)
  • In the work presented here, we used a codon-optimized firefly luciferase reporter system for detecting C. albicans in mice. (frontiersin.org)
  • It is the substrate of luciferase (EC 1.13.12.7), which is responsible for the characteristic yellow light emission from many firefly species. (wikipedia.org)
  • Modification of the firefly luciferin substrate has led to "red-shifted" emissions (up to emission wavelength of 675 nm). (wikipedia.org)
  • The longest emission wavelength of the synthesized luciferin analogues was recorded at 675 nm, which is within the NIR region. (eurekaselect.com)
  • However, the emission maximum ( λ max ) of bioluminescence produced by D -luciferin is 562 nm where light is not highly penetrable in biological tissues. (nature.com)
  • The emission maximum ( λ max ) of bioluminescence produced by D -luciferin is 562 nm. (nature.com)
  • This substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors and emits a characteristic yellow-green emission i. (gbiosciences.com)
  • Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (λmax = 613nm). (thermofisher.com)
  • We adapted the firefly luciferase in order to improve its maximum emission intensity in the red light range (600-700 nm) as well as to improve its thermostability in mice. (frontiersin.org)
  • Professor Branchini has worked with undergraduates, research assistants and research scientists for approximately 30 years investigating the basic biochemistry leading to the emission of light by the firefly. (conncoll.edu)
  • Different species of fireflies all use the same luciferin, however the color of the light emitted can differ greatly. (wikipedia.org)
  • Firefly lights differ between species and function in courtship, as a way for compatible insects to find one another. (reference.com)
  • Some species of firefly make their own bioluminescent enzymes, while others eat the self-sufficient species to obtain their enzymes. (reference.com)
  • These fireflies mimic the flashes of their prey species as a lure. (reference.com)
  • There are other firefly species flashing at night, and some of them are predatory, so she must be able to recognize males of her species. (nps.gov)
  • Now it gets more difficult, for there are many species of fireflies and each has its own flash pattern, which can vary in color, brightness and timing. (adirondackalmanack.com)
  • These fireflies mimic the flashes of other species and when males, thinking they're going to mate get close enough, they get eaten instead. (blueridgeoutdoors.com)
  • Each species of firefly has a characteristic flash pattern that helps its male and female individuals recognize each other. (bellaonline.com)
  • For a couple of weeks in early summer in mid June (4ththru the 12th), a rare species of fireflies come into the Smoky Mountains to do synchronized blinking. (yourcabin.com)
  • This species has an internal sensor that lets them know when another firefly is lit. (yourcabin.com)
  • Vargula hilgendorfii, sometimes called the sea-firefly and one of three bioluminescent species known in Japan as umi-hotaru (海蛍), is a species of ostracod crustacean. (wikipedia.org)
  • Single mutants (C62S, C62V, C86S, C146S, C164S), double mutants (C62/146S, C62/164S, C86/146S, C146/164S), and triple mutant C62/146/164S of the Luciola mingrelica firefly luciferase carrying C-terminal His6-tag were obtained on the basis of plasmid pETL7 by site-directed mutagenesis. (springer.com)
  • Bioluminescence comes in a range of greens, reds and blues, and it's caused by a protein called luciferin , often found in marine animals, mushrooms, insects, algae and specific types of bacteria. (merriam-webster.com)
  • Cao and Christopher Contag, PhD, assistant professor of pediatrics, radiology, microbiology and immunology, and lead author of the paper, were able to follow the transplanted cells' travels because they all made a firefly protein called luciferase. (brightsurf.com)
  • Fusion protein corresponding to Firefly Firefly Luciferase. (abcam.com)
  • Reacts with Luciferase (Firefly) Protein. (abcam.com)
  • The firefly produces two of these chemicals: luciferin and luciferase. (adirondackalmanack.com)
  • In fireflies, two chemicals, luciferin and luciferase, combine with the ATP in their cells to produce light. (neogen.com)
  • This pale green light serves as a warning to predators, since firefly larvae contain chemicals that are distasteful or toxic to animals and humans. (livescience.com)
  • In contrast, the PHIs FG-4592 (roxadustat) and FG-2216 (ICA, BIQ, IOX3, YM 311) did not affect firefly luciferase. (uzh.ch)
  • The NIEHS hopes to insert luciferase-producing genes from fireflies into the eggs of zebrafish. (newscientist.com)
  • And recently, a team at Cambridge University implanted firefly and photoluminescent bacteria genes into E.coli, an organism popular in synthetic biology because it is robust, fast-producing and can be modified into industrial strains. (ecmag.com)
  • Given that luciferin is delivered via the blood vessels, the researchers set out to determine the kinetics of luciferin delivery and whether BLI techniques could be used to gauge the effectiveness of drugs that destroy blood vessels that feed tumors. (photonics.com)
  • It was found that the luciferyl adenylate (the AMP ester of luciferin) spontaneously emits light in solution. (wikipedia.org)
  • Akalumine-HCl emits light in the NIR wavelength ranges ( λ max =677 nm) in reactions with native Fluc and yields increased detection sensitivity from deep-tissue targets with maximal signals attained at very low concentrations, as compared with D -luciferin and CycLuc1. (nature.com)
  • The Brazilian team used cloned railroad worm luciferase, which naturally emits red light and mutants of the enzyme together with a larger analog of luciferin synthesized by the Japanese team. (eurekalert.org)
  • Firefly luciferin is a substrate of the ABCG2 transporter and has been used as part of a bioluminescence imaging high thoroughput assay to screen for inhibitors of the transporter. (wikipedia.org)
  • Discovery of 5-benzyl-3-phenyl-4,5-dihydroisoxazoles and 5-benzyl-3-phenyl-1,4,2-dioxazoles as potent firefly luciferase inhibitors. (harvard.edu)
  • This study provides insights into a previously unknown JNJ-1935 off-target effect as well as into the chemical requirements for firefly luciferase and PHD inhibitors and may inform the development of novel compounds targeting these enzymes. (uzh.ch)
  • The technique requires a substrate called luciferin to be added to the bloodstream, which carries it to cells throughout the body. (photonics.com)
  • Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit. (fishersci.ca)
  • Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. (fishersci.ca)
  • Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. (thermofisher.com)
  • In this review, recent studies describing synthetic luciferin analogues predicted to have red-shifted bioluminescence are summarized. (eurekaselect.com)
  • The bioluminescence produced by AkaLumine-HCl in reactions with native firefly luciferase is in the near-infrared wavelength ranges (λ max =677 nm), and yields significantly increased target-detection sensitivity from deep tissues with maximal signals attained at very low concentrations, as compared with D -luciferin and emerging synthetic luciferin CycLuc1. (nature.com)
  • Luciferase Activity of Insect Fatty Acyl-CoA Synthetases with Synthetic Luciferins. (umassmed.edu)
  • The procedure was an acid-base extraction, given the carboxylic acid group on the luciferin. (wikipedia.org)
  • We synthesised an extended luciferin and found that it gave red shifted bioluminescence of λmax 662 nm with wild type luciferase, which is competitive with the most red shifted analogues to date. (ucl.ac.uk)
  • Especially, the genic basis of luciferin ( d -luciferin) biosynthesis and light patterns is largely unknown. (nature.com)
  • Most seriously, the genic basis of luciferin biosynthesis is largely unknown. (nature.com)
  • Firefly luciferin biosynthesis appears not to be known. (igem.org)
  • When you activate the device, typically by pushing the plunger through the cartridge, the ATP on the sampling tip mixes with luciferin and luciferase contained on a reagent pad or in a liquid mixture. (neogen.com)
  • Molecular characterization of firefly nuptial gifts: a multi-omics approach sheds light on postcopulatory sexual selection. (harvard.edu)
  • Conti E, Franks NP, Brick P (1996) Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. (springer.com)
  • Their work, "Designing Quantum Rods for Optimized Energy Transfer with Firefly Luciferase Enzymes," was published online May 23 in Nano Letters and is forthcoming in print. (innovationtoronto.com)
  • The annual firefly viewing opportunity for Great Smoky Mountains has ended for the 2021 season. (nps.gov)
  • The bugs we saw in Tennessee were the Synchronous Elkmont Fireflies, which can be found in the Smoky Mountains. (bellaonline.com)
  • Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. (biotium.com)
  • Our results demonstrate that JNJ-1935 is a novel inhibitor of firefly luciferase enzymatic activity. (uzh.ch)