Firefly and renilla luciferase. Medical search. Frequent questions

The targeting relationships between miR-9-3p and FOXC1/FAM171A1, miR-135b-3p and RGMA were validated by dual-luciferase reporter assays. (nature.com)
These features make these luciferases ideal for repeated assays, sensitive measurements in miniaturized assays and single cell applications. (neb.com)
The 3′UTR-binding sequence site of miR-128-5p on the p21 gene was predicted and assessed by luciferase assays. (hindawi.com)
Additional concentrated cell lysis buffer that is designed for use with Biotium's Firefly Luciferase Assay Kits when performing large sample volume assays. (biotium.com)
The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors. (addgene.org)
Luciferase reporter assays demonstrated that the Adcy1 promoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1. (jneurosci.org)
To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
Microarrays followed by bioinformatic analyses, luciferase reporter assays and Western blotting were applied for mechanistic studies. (thno.org)
A luciferase reporter assay, RNA immunoprecipitation (RIP), RNA pulldown assays and fluorescence in situ hybridization were performed to identify the interactions among circIGF2BP3, miR-328-3p, miR-3173-5p and plakophilin 3 (PKP3). (biomedcentral.com)

Scientists have developed a wide range of luciferase-based plasmid systems for many different applications. (addgene.org)
Three vector constructs (the unmodified plasmid psiCHECK-2-TTG and plasmids psiCHECK-2-α and -β) were transfected into HEK293 cells, and the firefly and Renilla luciferase activities were measured 24 h after transfection. (molvis.org)
The ratio of Renilla and firefly luciferase activity was normalized against the unmodified plasmid psiCHECK-2-TTG. (molvis.org)
The relative ratio of Renilla /firefly luciferase activity determined from cells transfected with the plasmid psiCHECK-2-α was set at 1. (molvis.org)
Cells were transfected with the miR-30ac2 expression plasmid "pSLIKG-miR30ac2") or a control plasmid ("pSLIKG-Scr"), plus the indicated luciferase reported plasmid. (figshare.com)
The firefly luciferase activity was used to normalize for transfection efficiency, and further correction was performed to control for the effects of the reporter plasmids and the miRNA expression plasmid. (figshare.com)

The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. (sigmaaldrich.com)
Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short luminescence half-life makes it too dim for many experimental systems. (addgene.org)
This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. (tebubio.com)
It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. (tebubio.com)
For miRNA target validation using GeneCopoeia's pEZX-MT05 and pEZX-MT06 target clones, our Secrete-Pair™ Dual Luminescence Assay Kit , Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Duo-Luciferase HS Assay Kit are the recommended assay kits respectively. (genecopoeia.com)
The simple 'add-and-read' workflow generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. (abpbio.com)

The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. (wikipedia.org)
However, unlike fluorescent proteins, luciferases do not require an external light source, but do require addition of luciferin, the consumable substrate. (wikipedia.org)
The substrate is then available for oxidation by the luciferase, where it is degraded to coelenteramide with a resultant release of energy. (wikipedia.org)
There is a large pocket in the β-barrel of the dinoflagellate luciferase at pH 8 to accommodate the tetrapyrrole substrate but there is no opening to allow the substrate to enter. (wikipedia.org)
Since light emission depends strictly on the chemical reaction between the substrate and the luciferase, there is no background light originating from the sample. (neb.com)
Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. (addgene.org)
A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo . (addgene.org)
TransDetect ® Double-Luciferase Reporter Assay Kit first uses luciferin as a substrate to detect the activity of the firefly luciferase reporter gene. (gaiascience.com.sg)
Then, while quenching the fluorescence reaction, the activity of the Renilla luciferase reporter gene was detected by using coelenterazine as a substrate. (gaiascience.com.sg)

Insect luciferases, including Photinus pyralis (firefly), catalyze the oxidation of a benzothiazol luciferin requiring ATP as a cofactor. (neb.com)
Firefly luciferase and Renilla luciferase can catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or coelenteramide, respectively, and produce bioluminescence in the process. (gaiascience.com.sg)

The luciferase activities in the A549 cells incubated at 33 or 37°C and the DF-1 cells incubated at 39°C were measured by using the Dual-Glo Luciferase Assay System (Promega) at 24 h post-transfection. (cdc.gov)
In addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of expression. (addgene.org)
The functional data of mutations were obtained by in vitro transfection and luciferase experiments and computer modelling. (biomedcentral.com)

Naturally secreted from the deep sea copepod Gaussia princeps (3) (Figure 1), Gaussia Luciferase (GLuc) was first used in a sensitive analytical assay in 2002 (4) and later commercialized by NEB for expression in mammalian cells. (neb.com)
GLuc is a monomeric enzyme of only 185 amino acids (19 kD), and is the smallest known luciferase. (neb.com)
Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and green Click-beetle luciferases (CBRluc and CBGluc, respectively). (addgene.org)
Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP). (genecopoeia.com)

Renilla Luciferase (RLuc), like most marine luciferases, uses a different luciferin, the benzylimidazo-pyrazinone coelenterazine. (neb.com)
The most common types of luciferase found in Addgene's collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc), and NanoLuc® Luciferase (Nluc). (addgene.org)

The Firefly Luciferase Reagent is first added to the cells in medium directly. (tebubio.com)
Next, the Renilla Luciferase Reagent is added to the same well. (tebubio.com)
Prepared Luciferase Reaction Reagent should be stored in aliquots in dark at -20°C for one month or at -70°C for one year. (gaiascience.com.sg)
The ABP Dual Luciferase Assay Kit is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. (abpbio.com)

The secreted luciferases derived from Gaussia princeps , Metridia longa and Cypridina noctiluca do not require cell lysis to measure activity and are significantly "brighter" than firefly and Renilla . (neb.com)

Also well studied is the sea pansy, Renilla reniformis. (wikipedia.org)
Later, a marine luciferase from the sea pansy Renilla reniformis was isolated. (neb.com)

The first generation of luciferase reporters developed were based on Firefly Luciferase (FLuc). (neb.com)

One well-studied luciferase is that of the Photinini firefly Photinus pyralis, which has an optimum pH of 7.8. (wikipedia.org)

This kit takes advantage of Firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. (sigmaaldrich.com)
In this organism, the luciferase (Renilla-luciferin 2-monooxygenase) is closely associated with a luciferin-binding protein as well as a green fluorescent protein (GFP). (wikipedia.org)
The N-terminal domain is conserved between dinoflagellate luciferase and luciferin binding proteins (LBPs). (wikipedia.org)

The target interaction between miR‑195‑5p and circ‑PVT1 or ETS1 was predicted through bioinformatics analysis, and verified by dual‑luciferase reporter gene assay. (spandidos-publications.com)
As targets of siRNA, the entire bPRNP coding sequence was connected to the reporter gene of the fluorescent EGFP, or of firefly luciferase or Renilla luciferase. (scientistlive.com)
Lentiviral particles with Firefly Luciferase as the reporter gene for binding of activated NFkB to NFkB-TRE. (nucleusbiotech.com)

Yeast one-hybrid (Y1H) experiments suggested that LsARF3 could physically interact with the LsCO promoter, which was further confirmed by a dual luciferase assay in tobacco leaves. (frontiersin.org)
Renilla l uciferase was expressed under CMV promoter, A Puromycin antibiotic marker was expressed under Rsv promoter. (gentarget.com)

Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. (tebubio.com)
For enhanced stability detection of Firefly and Renilla luciferase activities. (genecopoeia.com)

The majority of studied luciferases have been found in animals, including fireflies, and many marine animals such as copepods, jellyfish, and the sea pansy. (wikipedia.org)

However, luciferases have been studied in luminous fungi, like the Jack-O-Lantern mushroom, as well as examples in other kingdoms including bioluminescent bacteria, and dinoflagellates. (wikipedia.org)
Light emission in some bioluminescent bacteria utilizes 'antenna' such as lumazine protein to accept the energy from the primary excited state on the luciferase, resulting in an excited lulnazine chromophore which emits light that is of a shorter wavelength (more blue), while in others use a yellow fluorescent protein (YFP) with flavin mononucleotide (FMN) as the chromophore and emits light that is red-shifted relative to that from luciferase. (wikipedia.org)
Recently, a new generation of bioluminescent reporters with unique characteristics that expand the flexibility and applications of luciferase reporter systems has become available. (neb.com)

The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. (tebubio.com)
The ABP Dual Luciferase Assay Kit is the best choice for cell-based, high-throughput screening applications. (abpbio.com)

The Metridia longa secreted luciferase gene encodes a 24 kDa protein containing an N-terminal secretory signal peptide of 17 amino acid residues. (wikipedia.org)
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression. (addgene.org)
Before inserting the 5′-UTR sequences into the psiCHECK-2 vector, the ATG of the Renilla luciferase was mutated to TTG, so that the Renilla luciferase expression would be driven by the primary ATG initiation codon (reverse triangle) of the gene under investigation. (molvis.org)

Firefly luciferase is considerably brighter than Renilla luciferase, but is more susceptible to enzyme inhibition. (addgene.org)

Luc2 is the second-generation version of Firefly luciferase that has been codon optimized for expression in mammalian systems. (addgene.org)
Like Firefly luciferase, Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems. (addgene.org)

One such example is the Metridia coelenterazine-dependent luciferase (MetLuc, A0A1L6CBM1) that is derived from the marine copepod Metridia longa. (wikipedia.org)

Although both of these intracellular luciferases are widely used in life science research, they have the disadvantage of requiring lysis of the expressing cells in order to measure their activity. (neb.com)
Polymerase activity was calculated by standardization of the firefly luciferase activity to the Renilla luciferase activity. (cdc.gov)
We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting. (bmj.com)
Luciferase activity. (molvis.org)
In the ABP Dual Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results. (abpbio.com)
Gremlin 1 activity is determined using inhibition of the BMP2 response (Qk007 #010, 52 ng/ml) from a BMP2-responsive firefly luciferase reporter in stably transfected HEK293T cells. (qkine.com)
Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. (qkine.com)

The catalyzed reaction is: coelenterazine + O2 → coelenteramide + CO2 + photon of light Newer luciferases have recently been identified that, unlike other luciferases, are naturally secreted molecules. (wikipedia.org)

Luciferases have become indispensable for the study of fundamental cellular processes including the regulation of transcription and translation, mRNA and protein stability, and nucleic acid/protein interactions. (neb.com)

With the ABP Dual Luciferase Assay System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. (abpbio.com)

Luciferases are widely used in biotechnology, for bioluminescence imaging microscopy and as reporter genes, for many of the same applications as fluorescent proteins. (wikipedia.org)

Dinoflagellate luciferase is a multi-domain eukaryote protein, consisting of an N-terminal domain, and three catalytic domains, each of which preceded by a helical bundle domain. (wikipedia.org)
Both Firefly luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. (addgene.org)
Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. (addgene.org)
NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available - these attributes have made it popular with scientists since its release. (addgene.org)

A variety of organisms regulate their light production using different luciferases in a variety of light-emitting reactions. (wikipedia.org)

Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. (addgene.org)

Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. (wikipedia.org)

Renilla and firefly luciferase activities were measured in cell exctracts. (figshare.com)

A minigenome assay based on the dual-luciferase system was performed as previously reported ( 2 , 3 ). (cdc.gov)
Next, nine HER3-CTD truncation mutants were constructed to map minimal regions of transactivation potential using the Gal4 UAS-luciferase based system. (plos.org)

Table of PITA and Targetscan scores (if available) for the indicated UTRs, with the corresponding luciferase expression data from Figure 2. (figshare.com)

Empty backbones for inserting regulatory elements before or after the luciferase ORF can be found in a table below, as well as plasmids that already contain a region of interest before or after luciferase . (addgene.org)

ATP Cell Viability Luciferase Assay A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability. (sigmaaldrich.com)
The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. (sigmaaldrich.com)

The targeting of NEK6 by miR-323a-3p was verified using a dual-luciferase reporter assay. (hindawi.com)

This light emission can be easily detected and measured, making luciferases ideal for the development of biological reporter systems. (neb.com)
A set of three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves as a convenient toolkit to assess the proteostasis status in a wide range of experimental systems. (addgene.org)

However, due to the closely associated GFP, the energy released by the luciferase is instead coupled through resonance energy transfer to the fluorophore of the GFP, and is subsequently released as a photon of green light (peak emission wavelength 510 nm). (wikipedia.org)

Anatomy1

Organisms4

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes11

Information Science2

Assays9

Plasmid6

Luminescence6

Substrate9

Catalyze the oxidation2

Transfection3

GLuc4

RLuc2

Reagent4

Gaussia1

Reniformis2

FLuc1

Photinus1

Luciferin3

Reporter gene3

Promoter2

Detection2

Pansy1

Bioluminescent3

High-throughput2

Gene3

Brighter1

Mammalian2

Metridia1

Activity7

Coelenterazine1

Stability1

Cytotoxicity1

Fluorescent1

Protein4

Reactions1

Enzyme1

Oxidative1

Activities1

System2

Expression1

Regulatory1

Highly sensitive2

Dual1

Systems2

Light1