Loading...
  • typically
  • The DNA-binding domains of individual ZFNs typically contain between three and six individual zinc finger repeats and can each recognize between 9 and 18 basepairs. (wikipedia.org)
  • see: Zinc finger chimera for more info on zinc finger selection techniques) The non-specific cleavage domain from the type IIs restriction endonuclease FokI is typically used as the cleavage domain in ZFNs. (wikipedia.org)
  • hand
  • Use your other hand to pull your affected finger beyond extension until you feel a stretch in the bottom of your finger. (livestrong.com)
  • Working against the resistance of the rubber band, open your hand by extending your fingers as much as possible. (livestrong.com)
  • procedure
  • The main drawback with this procedure is the specificities of individual zinc fingers can overlap and can depend on the context of the surrounding zinc fingers and DNA. (wikipedia.org)
  • Without methods to account for this "context dependence", the standard modular assembly procedure often fails unless it is used to recognize sequences of the form (GNN)N. Numerous selection methods have been used to generate zinc-finger arrays capable of targeting desired sequences. (wikipedia.org)
  • gene
  • Zinc finger nucleases have also been used in a mouse model of haemophilia and a clinical trial found CD4+ human T-cells with the CCR5 gene disrupted by zinc finger nucleases to be safe as a potential treatment for HIV/AIDS. (wikipedia.org)
  • sequence
  • If the zinc finger domains perfectly recognize a 3 basepair DNA sequence to generate a 3-finger array that can recognize a 9 basepair target site. (wikipedia.org)
  • This system combines pre-selected pools of individual zinc fingers that were each selected to bind a given triplet and then utilizes a second round of selection to obtain 3-finger arrays capable of binding a desired 9-bp sequence. (wikipedia.org)