• Zebrafish fin regeneration requires the formation and maintenance of blastema cells. (elsevierpure.com)
  • Blastema cells are not derived from stem cells but behave as such, because they are slow-cycling and are thought to provide rapidly proliferating daughter cells that drive regenerative outgrowth. (elsevierpure.com)
  • We used a chemical mutagenesis screen to identify no blastema (nbl), a zebrafish mutant with an early fin regeneration defect. (elsevierpure.com)
  • Fin regeneration failed in nbl due to defective blastema formation. (elsevierpure.com)
  • Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
  • Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
  • Zebrafish have the remarkable ability to regenerate body parts including the heart and fins by a process referred to as epimorphic regeneration. (oregonstate.edu)
  • however, our results suggest that it is also required for early stages of tissue regeneration. (oregonstate.edu)
  • While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)
  • This study demonstrates the importance of a stress response for stem cell activation and regeneration in a basal chordate, which may have implications for understanding the limited regenerative activities in other animals, including vertebrates. (biomedcentral.com)
  • Recent studies have illustrated that similar to adult zebrafish, early life stage larvae also possess the ability to regenerate the caudal fin. (oregonstate.edu)
  • This work presents a promising approach to develop biomimetic, nanofibrillar soft hydrogels for regenerative medicine applications. (bvsalud.org)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • These data indicate that hsp60 is required for the formation and maintenance of regenerating tissue. (elsevierpure.com)
  • Adult zebrafish fins fully regenerate after resection, providing a highly accessible and remarkable vertebrate model of organ regeneration. (bvsalud.org)
  • Further, we establish zebrafish fin development and regeneration as an accessible model to explore mechanisms of Fraser Syndrome-associated digit defects and Fraser Complex function at epithelial-mesenchymal interfaces. (bvsalud.org)
  • Zebrafish robustly regenerate fins, including their characteristic bony ray skeleton. (bvsalud.org)
  • Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis. (nih.gov)
  • Moreover, a dominant-negative approach in chick and knockdown analysis with morpholinos in zebrafish revealed their requirement for Fgf8 expression and limb outgrowth, and further indicate that they have a coordinated action on Fgf8 expression. (biologists.com)
  • fras1 mutant fins regenerate to their original size but with exacerbated ray branching and fin symmetry defects. (bvsalud.org)
  • Adult zebra fish completely regenerate their caudal (tail) fin following partial amputation. (oregonstate.edu)
  • During its tadpole stages, Xenopus can regenerate most tissues including its spinal cord however this ability is lost after metamorphosis, allowing comparative studies within one species. (manchester.ac.uk)
  • The drug fingolimod improved peripheral nerve regeneration in preclinical rodent models by stimulating a pro-regenerative Schwann cell phenotype and axonal growth. (frontiersin.org)
  • Furthermore, immunohistochemical staining revealed that axonal outgrowth was impacted by TCDD as early as 3 dpa. (oregonstate.edu)
  • Approximately 10% of fras1 mutants survive to adulthood, displaying striking and varied fin abnormalities, including endochondral bone fusions, ectopic cartilage, and disrupted caudal fin symmetry. (bvsalud.org)
  • Our study demonstrates that Sp8 and Sp9 , via Fgf8 , are involved in mediating the actions of Fgf10 and Wnt/β-catenin signaling during vertebrate limb outgrowth. (biologists.com)
  • Antibody-based staining of sectioned fins facilitates such high-resolution analyses of specific, native proteins. (bvsalud.org)
  • We propose Fraser Complex-supported tissue layer adhesion enables robust integrated tissue morphogenesis involving the basal epidermis and osteoblasts. (bvsalud.org)
  • Therefore, there is a high requirement for improving regenerative capabilities of the spinal cord after injury. (manchester.ac.uk)
  • We show that two closely related buttonhead -like zinc-finger transcription factors, Sp8 and Sp9 , are expressed in the AER, and regulate Fgf8 expression and limb outgrowth. (biologists.com)
  • Understanding the molecular mechanisms that control how outgrowth of different tissues and organs of the embryo are established and maintained is one of the major questions in the study of embryonic development. (biologists.com)
  • Histological analysis demonstrated that TCDD exposure leads to an accumulation of collagen at the end of the fin ray just distal to the amputation site by 3 dpa. (oregonstate.edu)
  • We demonstrate that sall4 and a related gene sall1 act downstream of tbx5 and are required for pectoral fin development. (nih.gov)
  • By 3 days postamputation (dpa), the vascular network in the regenerating fin of TCDD-exposed fish was disorganized compared to vehicle-exposed animals. (oregonstate.edu)
  • Fin injury triggers wound epidermis formation and the dedifferentiation of injury-adjacent mature cells to establish an organized blastema of progenitor cells. (bvsalud.org)