Field of fluorescence. Medical search. Frequent questions

Nonetheless, obtaining reliable, quantitative spatial information from conventional wide-field optical microscopy is not always intuitive as it appears to be. (caltech.edu)
In other words, conventional wide-field optical microscopy transforms the three-dimensional spatial information, or volumetric information about the objects into a two-dimensional form in each acquired image, and therefore distorts the spatial information about the object. (caltech.edu)
We present deep-learning-enabled super-resolution across different fluorescence microscopy modalities. (nature.com)
We further demonstrate that total internal reflection fluorescence (TIRF) microscopy images of subcellular structures within cells and tissues can be transformed to match the results obtained with a TIRF-based structured illumination microscope. (nature.com)
Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. (nature.com)
Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. (nature.com)
A16.0203 Epi-fluorescent microscope is used for fluorescence microscopy and transmitted field observation. (cnoec.com)
The diversification of the original FISH protocol into the impressive number of procedures available these days has been promoted throughout the years by a number of interconnected factors: the improvement in sensitivity, specificity and resolution, together with the advances in the fields of fluorescence microscopy and digital imaging, and the growing availability of genomic and bioinformatic resources. (biosyn.com)
Part II included guest lectures on fluorescence microscopy, multi-photon microscopy, correlation spectroscopy, single molecule detection and fluorescence markers as well as high-throughput screening. (picoquant.com)
Graphics & Web Programming Team in collaboration with Optical Microscopy at the National High Magnetic Field Laboratory . (fsu.edu)
The immersion oil is specially formulated for reducing autofluorescence that is typically associated with oils, making it an ideal choice for Fluorescence Microscopy applications. (edmundoptics.com)
In this thesis we explore the possibilities of homo-FRET induced fluorescence anisotropy microscopy, and in particular we compare mCherry and GFP as labeling proteins. (uu.nl)
Furthermore this thesis serves as a simple guide to homo-FRET and fluorescence anisotropy microscopy, and includes an extensive measurement and data analysis protocol. (uu.nl)
Researchers seeking to view nuclei, stem cells, certain brain slices, neuron signaling, DNA and chromosomes often use wide-field epifluorescence microscopy. (photonics.com)
For the researcher interested in imaging specimens at mid-depth, wide-field fluorescence microscopy can offer beautiful images and excellent data at a relatively low price point (Figure 2). (photonics.com)
Fluorescence microscopy meets all the essential requirements for laboratory examination even under constrained conditions of work. (who.int)
Fluorescence microscopy has proved to be a useful and very cost-effective procedure for disease surveillance and for the diagnosis of many infectious diseases caused by bacteria, viruses or protozoa, as well as noncommunicable diseases in hospital and outpatient care. (who.int)
Fluorescence microscopy is almost as simple to do as bright-field microscopy, and most often it is more specific. (who.int)

Fluorescence cross-correlation spectroscopy ( FCCS ) is a spectroscopic technique that examines the interactions of fluorescent particles of different colours as they randomly diffuse through a microscopic detection volume over time, under steady conditions. (wikipedia.org)
Eigen and Rigler first introduced the fluorescence cross-correlation spectroscopy (FCCS) method in 1994. (wikipedia.org)
FCCS is an extension of the fluorescence correlation spectroscopy (FCS) method that uses two fluorescent molecules instead of one that emits different colours. (wikipedia.org)
They attended 18 lectures and 3 hands-on experiment sessions with 10 different instruments from 5 companies, covering different methods and procedures in fluorescence spectroscopy. (picoquant.com)
The course was intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. (picoquant.com)
Part I covered the basics of steady state and time-resolved fluorescence spectroscopy and instrumentation, and was designed for individuals who are not experts. (picoquant.com)
Jörg Enderlein,Head of the Single Molecule Detection group at FZ Juelich and specializes in Single Molecule Detection, fluorescence correlation spectroscopy and complex data analysis. (picoquant.com)
The European short course on 'Principles and Applications of Time-resolved Fluorescence Spectroscopy' is an annual event since 2003. (picoquant.com)
Articles, abstracts, and book chapters citing Globals Software (Globals Unlimited, Globals for Spectroscopy, and Globals for Images aka SimFCS) by authors not affiliated with the Laboratory for Fluorescence Dynamics (LFD). (uci.edu)
Fluorescence Spectroscopy. (nih.gov)
In this work, the impact of microorganisms isolated from a Waste Water Treatment Plant (WWTP) was investigated to determine the fate of NOM fractions in raw sewage, using fluorescence spectroscopy. (scirp.org)
The incubated waters were analyzed by fluorescence spectroscopy, digitally resolved into NOM components: humic- and fulvic-like, and two types of protein-like, i.e ., tryptophan- and tyrosine-like, using a Parallel Factor Analysis routine (PARAFAC). (scirp.org)
1. Fluorescence spectroscopy: a diagnostic tool for cervical intraepithelial neoplasia (CIN). (nih.gov)
15. Detection and evaluation of normal and malignant cells using laser-induced fluorescence spectroscopy. (nih.gov)

UAV-based sun-induced chlorophyll fluorescence was acquired over potato and sugar beet fields during clear sky conditions on August 2. (4tu.nl)
Reliability and comparability of chlorophyll fluorescence data from several field teams" [Environ. (unicatt.it)

For imaging just below the surface of a specimen, many scientists choose total internal reflection fluorescence (TIRF) imaging (Figure 1). (photonics.com)

This paper describes the preliminary measurements of the temperature field in water channel by the Planar Laser-Induced Fluorescence (pLIF) method in the one-colour mode using commercial LaVision system. (epj-conferences.org)

Single fluorophores, both in polymer and covalently attached to amino- silanized glass, are imaged using two-channel fluorescence polarization detection. (utwente.nl)
Part II emphasised time-resolved fluorescence, together with advanced applications of fluorescence in biophysics, sensing, imaging, clinical chemistry, multi-photon excitation and ultrasensitive detection. (picoquant.com)
Turn out all other lights for the clearest detection of fluorescence. (wood-database.com)
Through this FOA, the National Cancer Institute (NCI) solicits innovative concepts that propose a path to clinical validation for in vivo 'intelligent' or activated optical fluorescence agents or probes with previously demonstrated capabilities for the detection of small tumors. (nih.gov)
This FOA thus supports translation of novel activated optical fluorescence agents for sensitive cancer detection in vivo . (nih.gov)
Silver ions enhanced AuNCs fluorescence as a turn-off nanoprobe for ultrasensitive detection of iodide. (americanelements.com)
The new sensing method based on ion regulation to enhance the detection sensitivity can extend to the appliance of other fluorescent materials in biosensing and biomedical field. (americanelements.com)

These advantages together show the potential of our innovations to be widely used for high-speed, volumetric fluorescence imaging of living biological systems. (caltech.edu)

By combining FCCS with a confocal microscope, the technique's capabilities are highlighted, as it becomes possible to detect fluorescence molecules in femtoliter volumes within the nanomolar range, with a high signal-to-noise ratio , and at a microsecond time scale. (wikipedia.org)
Molecules from the sample matrix, target cells or reagents added during sample preparation that affect the in vitro DNA polymerization or the fluorescence signal are collectively called PCR inhibitors. (springer.com)
We demonstrate fluorescence imaging of single dye molecules in the illumination mode, achieving a spatial resolution less than 10 nm. (elsevierpure.com)
Organic molecules capable of thermally activated delayed fluorescence (TADF) have drawn great interest in the field of optoelectronic devices. (logos-verlag.de)
Biological specimens typically emit fluorescence signals three to six orders of magnitude less than the illumination signal, making it challenging to detect molecules of interest, particularly at greater depths. (photonics.com)

HPLC-based chromatography (Bldg. 10, Rm 8N117), and currently features 1) ESI-LC/MS systems and HPLC systems with UV/fluorescence detectors and 3) Atomic absorption spectrometer. (nih.gov)

We offer a full range of spectrometer upgrades for the FLS980 including configuration, light source and software upgrades in addition to monochromator options, detector option and fluorescence and phosphorescence upgrades. (edinst.com)
Dissolve 10.3 g of 97% pure reagent (page 5032-1), and a fluorescence detector. (cdc.gov)
The intensity of the scattered signal as a function of position, usually measured on a bucket detector with a single degree of freedom, gives information on the permittivity distribution of the sample [ 7 ] or on the optical near field supported by the sample. (degruyter.com)

While the age of bitumen increases, its fluorescence emission intensity decreases. (bentham.co.uk)
The test field confirmed the connection between intensity and ageing time, further the development of an oxidation gradient over the vertical cross section of the asphalt slabs could be observed. (bentham.co.uk)
In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata. (nature.com)
Our method enables objective assessment of stomatal dynamics by simple monitoring of the fluorescence intensity of HoeAc 2 Fl in the nucleus of the stomata. (nature.com)
Therefore, we proposed that HoeAc 2 Fl could constitute a useful chemical tool for the assessment of stomatal dynamics by simple monitoring of the intensity of the fluorescence of the nuclei of the guard cells. (nature.com)
The exact correlation between fluorescence intensity and stomatal aperture showed that the visible fluorescence was only observed from stomata with an aperture of less than about 2.5-3 µm (Fig. 2c , three representative images of closed or open stomata are shown in Fig. S1 , and fluorescence images of various stomata having different apertures are shown in Fig. S2 ). (nature.com)
Fluorescence intensity is also used to monitor amplicon generation in qPCR and dPCR. (springer.com)
Species that are shown to produce a fluorescent response may still vary from piece to piece in the color and intensity of the fluorescence. (wood-database.com)
In the presence of Ag, the fluorescence (FL) intensity of AuNCs can be enhanced obviously. (americanelements.com)
In this work, we present an ultrasensitive "turn-on" nanosensor for neuromodulators dopamine and norepinephrine with strong relative change in fluorescence intensity (Δ F/ F) of up to 3500%, a signal appropriate for in vivo neuroimaging, and uncover the photophysical principles and intermolecular interactions that govern the molecular recognition and fluorescence modulation of this nanosensor synthesized from the spontaneous self-assembly of (GT) ssDNA rings on SWNTs. (janelia.org)

Fluorescence spectroscopic investigation of bitumen aged by field exposure respectively modified rolling thin film oven test. (bentham.co.uk)
3. Smartphone-based fluorescence spectroscopic device for cervical precancer diagnosis: a random forest classification of in vitro data. (nih.gov)
10. Native Fluorescence and Time Resolved Fluorescence Spectroscopic Characterization of Normal and Malignant Oral Tissues Under UV Excitation--an In Vitro Study. (nih.gov)

Fluorescence in situ hybridization labels o-n repetitive and single copy probes o-n human metaphase chromosomes are imaged with a width of 80 nm, allowing their localization with nanometer accuracy, in direct correlation with the simultaneously obtained topography. (utwente.nl)
The introduction of FISH (fluorescence in situ hybridization) marked the beginning of a new era for the study of chromosome structure and function. (biosyn.com)

When the reflected illumination light is blocked with an absorbing filter, it is possible to visualize the longer wavelength fluorescence even with the naked eye. (medscape.com)
Otto S. Wolfbeis,Professor and Vice Rector/President at Regensburg university and specializes in design and synthesis of long-wavelength and long-lifetime fluorescent bioprobes and on fluorescence based optical sensors. (picoquant.com)
In fluorescence imaging, the specimen is illuminated by a specific wavelength of light that is absorbed by fluorophores attached to it. (photonics.com)

Until recently, this type of procedure has required a microscope to help the surgeon visualize the fluorescence. (mddionline.com)
A TIRF system typically includes a research-grade fluorescence microscope, together with a specialized optics and laser. (photonics.com)
The Zeiss Lumar is a high-quality stereo-microscope equipped with transmitted and reflected light imaging, together with regular fluorescence. (nih.gov)

A modified RTFOT was conducted to study the effects of different temperatures and operating times on the fluorescence emission spectrum, as well as the influence of air (containing traces of atmospheric radicals) by conducting the test with nitrogen. (bentham.co.uk)
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. (wikipedia.org)
Fluorescence occurs when an excited molecule, atom, or nanostructure, relaxes to a lower energy state (usually the ground state) through emission of a photon without a change in electron spin. (wikipedia.org)
Fluorescence is the emission of a photon accompanying the relaxation of the excited state to the ground state. (wikipedia.org)
The Olympus Empty Filter Cubes can accept 25mm diameter excitation and emission filters and 25.2 x 35.6mm dichroic filters for customized fluorescence configurations. (edmundoptics.com)
However, it sometimes can be challenging to generate enough fluorescence emission. (photonics.com)

The aim of the work is to find suitable parameters of the experiment (concentration of fluorescent dye, laser power, arrangement of the experiment, etc.) for measuring the temperature field in a closed glass channel of rectangular cross-section with a heated bottom-wall and to verify an applicability of this method in general. (epj-conferences.org)
as described later, this loss of fluorescence is largely attributed to a decrease in fluorescent crosslinks associated with stromal collagen that underlies the neoplastic lesion. (medscape.com)
But since fluorescence is due to a specific chemical, which can also be synthesized artificially in most cases, it is sufficient to describe the substance itself as fluorescent. (wikipedia.org)
This phenomenon is known as fluorescence, and certain woods can be distinguished by the presence or absence of their fluorescent qualities. (wood-database.com)
While some species will give a strong fluorescent response, many others will only be faintly fluorescent, while the majority of species will exhibit no fluorescence at all. (wood-database.com)

An introduction to the basic principles of field measurements of fluorescence is provided, as well as technical background information on sensors that target dissolved organic matter, wastewater, and hydrocarbons, including sensor selection, operating principles, key features, and design elements. (usgs.gov)
All these techniques are based on in vitro DNA polymerization and fluorescence measurements. (springer.com)
A Parrot Sequoia + collected multispectral measurements over the two crop fields around 11:00 on the same day. (4tu.nl)
So unlike s-NSOM, where spatial information is obtained from consecutive measurements while raster-scanning a detecting element, here we exploit the complexity and connection of near and far fields to obtain spatial information from a single measurement. (degruyter.com)
Methods considered real-time generally rely on field-portable instruments that give instantaneous measurements, often as a visual display on the body of the instrument. (cdc.gov)

Because both the reflected excitatory light and emitted fluorescence are collected along the same optical path, epifluorescence produces a high signal-to-noise ratio. (photonics.com)

We demonstrate, theoretically and experimentally, that our proposed wide-field optical-sectioning techniques can achieve diffraction-limited optical sectioning, low out-of-focus excitation and high-frame-rate imaging in living biological systems. (caltech.edu)
Presentations wil be focused on transformative technologies such as longitudinal MRI, lipid-exchanged, anatomically rigid, imaging/immunostaining compatible, tissue hydrogel (CLARITY), De-scattering with Excitation Patterning (DEEP) and two-photon fluorescence imaging. (nih.gov)

Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc 2 Fl) for the qualitative assessment of stomatal dynamics. (nature.com)

Be careful not to mistake simple reflections or illumination of the wood surface for fluorescence: the wood should literally glow. (wood-database.com)

The FLS1000 is a state of the art spectrometer for the most demanding fluorescence analysis applications in Photophysics, Photochemistry, Materials Sciences and Life Sciences. (edinst.com)

The use of field deployable fluorescence sensors by the U.S. Geological Survey has become increasingly common for a wide variety of surface water and groundwater investigations. (usgs.gov)
An appendix is included to describe sensor calibration criteria and procedures, reporting units, and specific approaches to correct for interferences for fluorescence of dissolved organic matter sensors. (usgs.gov)

In the case of near real-time monitoring, advances in developing field portable versions of what were previously laboratory methods have expanded capabilities for short turnaround times between sample collection and analysis. (cdc.gov)

In addition to such imaging capabilities, our proposed techniques can be instrumentally simple and economic, and are straightforward for implementation on conventional wide-field microscopes. (caltech.edu)

PCR inhibition affects library preparation in MPS analysis and skews quantification in qPCR, and some inhibitors have been found to quench the fluorescence of the applied fluorophores. (springer.com)

Riopel, R. , Siemann, S. and Caron, F. (2016) Microbial Changes in the Fluorescence Character of Natural Organic Matter from a Wastewater Source. (scirp.org)

A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. (illinois.edu)
Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. (illinois.edu)
Manzella-Lapeira J, Brzostowski J. Fluorescence Lifetime Imaging as a Noninvasive Tool to Study Plasmodium Falciparum Metabolism . (nih.gov)

Beyond the angle of total reflection, the electromagnetic field of the incoming/reflected light extends into the Z axis, but the strength of this evanescent wave decreases exponentially as it extends through the second medium. (photonics.com)
Fluorescence outside the evanescent field is minimal, providing high-contrast images with good signal-to-noise ratios at the right depth. (photonics.com)
An alternative approach to construct images with subwavelength resolution is by detecting the evanescent optical fields close to the sample that contain high-frequency spatial information. (degruyter.com)

Fluorescence also occurs frequently in nature in some minerals and in many biological forms across all kingdoms of life. (wikipedia.org)

The K(d)s (dissociation constants) of 21 flavone derivatives have been obtained by fluorescence in vitro when binding with Aβ(1-40) (β-amyloid(1-40)) aggregates protein. (unboundmedicine.com)

Fluorophores within the oral epithelium and stroma absorb UV and visible light and can re-emit some of this light at longer wavelengths in the form of fluorescence. (medscape.com)

We summarize the recent progress with the "covalent-assembly" design principle, which warrants high-performance fluorescence probes exhibiting a highly sensitive turn-on signal from the dark background. (rsc.org)

In the absence of spectral bleed-through -when the fluorescence signal from an adjacent channel is visible in the channel being observed-, the cross-correlation function is zero for non-interacting particles. (wikipedia.org)

Several fluorescence holography-based methods have demonstrated the ability to obtain three-dimensional information about the objects, but these methods generally rely on decomposing stereoscopic visualizations to extract volumetric information and are unable to resolve complex 3-dimensional structures such as a multi-layer sphere. (caltech.edu)
There are several novel optical fluorescence methods that rely on the use of specialized agents that are activated when coupled to a tumor target. (nih.gov)
Direct-reading exposure assessment methods may be either real-time (instantaneous readings) or near real-time (field-based analysis providing shorter turnaround times between sample collection and result determination than is typical with laboratory analysis). (cdc.gov)

described an initial version of this device for the direct visualization of oral cavity tissue fluorescence. (medscape.com)
The arrow indicates the region of fluorescence visualization loss and biopsy location. (medscape.com)

Knowledge is collected from the fields of forensics, clinical diagnostics, food safety and veterinary medicine. (springer.com)

Why not view our complete range of fluorescence analysis instrumentation and find the best instrument for your fluorescence research. (edinst.com)
For further information on our range of fluorescence analysis instrumentation, why not visit our fluorescence products overview . (edinst.com)

By monitoring the scattering pattern of the nanophotonic near-field transducer and comparing it to measured reference data, we demonstrate the two-dimensional localization of the object accurate to 24 nm across an area of 2 × 2 μm. (degruyter.com)

Attendees were typically professionals who are using or intend to use fluorescence in their research. (picoquant.com)
Most attendees had some knowledge of fluorescence, typically in a specialized area. (picoquant.com)

In order to overcome such an issue, several wide-field optical-sectioning techniques have been proposed and demonstrated, although not without introducing new limitations and compromises such as low signal-to-background ratios and reduced axial resolutions. (caltech.edu)
In order to develop wide-field optical-sectioning techniques that has equivalent optical performance as single-point-scanning ones, this thesis first introduces the mechanisms and limitations of existing wide-field optical-sectioning techniques, and then brings in our innovations that aim to overcome these limitations. (caltech.edu)
Using this framework, we improve the resolution of wide-field images acquired with low-numerical-aperture objectives, matching the resolution that is acquired using high-numerical-aperture objectives. (nature.com)
It is equipped with infinity plan achromatic objectives and wide field eyepieces, have clear picture and wide view field. (cnoec.com)
The four socket head cap screws must be removed to mount the #36-401 Olympus Wide Field Tube Lens in the #11-149 Olympus Wide Field Tube Lens to T-Mount Holder. (edmundoptics.com)

In 1819, E.D. Clarke and in 1822 René Just Haüy described fluorescence in fluorites, Sir David Brewster described the phenomenon for chlorophyll in 1833 and Sir John Herschel did the same for quinine in 1845. (wikipedia.org)
He named this phenomenon fluorescence "I am almost inclined to coin a word, and call the appearance fluorescence, from fluor-spar [i.e., fluorite], as the analogous term opalescence is derived from the name of a mineral. (wikipedia.org)

This course will be of interest to staff developing XRF sampling and analysis plans, reviewing the plans for quality assurance, field operators, and users of XRF data for making project decisions. (clu-in.org)

By measuring the spectrum of fractions, and by correlating fractional changes during ageing within bitumen with the corresponding spectra, a connection between bitumen's age and the fluorescence spectrum can be drawn. (bentham.co.uk)

We carried out non-destructive thickness and composition analysis of Zinc-Nickel coating on steel using the MESA-50 X-ray fluorescence analyzer. (horiba.com)
We tried the analysis of the field of view of the telescope using this system. (sissa.it)
This critical review provides an overview of sensitivity-enhancement strategies and a systematic, quantitative analysis of field-effect transistor (IS-FET/BioFET) sensor literature. (rsc.org)
8. Enhancement of early cervical cancer diagnosis with epithelial layer analysis of fluorescence lifetime images. (nih.gov)

This report addresses field deployable fluorometers that measure the fluorescence response of various substances in water exposed to incident light generated by the sensor. (usgs.gov)
In contrast, when the stomata were treated in the light, almost no fluorescence was observed from HoeAc 2 Fl-stained stomata (Fig. 2b ). (nature.com)
We have developed a standard UV-LED light source mounted on an unmanned aerial vehicle (UAV), so-called the Opt-copter, for the calibration of the Fluorescence Detectors (FDs) of the Telescope Array experiment. (sissa.it)
We aim to construct an optical near-field transducer in the form of a nanophotonic target structure that determines the position of a nanoscale perturbation located near the structure on basis of collected scattered light. (degruyter.com)

CDC researchers have developed improved Loop-Mediated Isothermal Amplification (LAMP) assays for the nucleic acid-based diagnosis of malaria in field settings. (nih.gov)

Iodine compounds are important in organic chemistry and very useful in the field of medicine . (americanelements.com)
Book Description: Céline Leonhardt : Novel Organic Emitters for Thermally Activated Delayed Fluorescence, Reihe: Beiträge zur organischen Synthese, Bd. (logos-verlag.de)

Moreover, 72% (80 out of 111) of lesions or conditions clinically characterized as inflammation, ulceration or pigmentation demonstrated a loss of fluorescence on VELscope examination, potentially making it difficult to distinguish them from neoplastic lesions, which also exhibit decreased autofluorescence (Figure 2). (medscape.com)

Anatomy11

Organisms8

Diseases4

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment70

Psychiatry and Psychology1

Phenomena and Processes63

Disciplines and Occupations11

Technology, Industry, Agriculture12

Information Science10

Health Care5

Microscopy18

Spectroscopy14

Chlorophyll fluorescence2

Total internal ref1

Laser-induced fluo1

Detection7

Volumetric fluorescence1

Molecules5

HPLC1

Detector3

Intensity10

Spectroscopic3

Situ hybridization2

Wavelength3

Microscope3

Emission6

Fluorescent5

Measurements5

Epifluorescence1

Excitation2

Dynamics1

Illumination1

Spectrometer1

Sensors2

Advances1

Microscopes1

Quench1

Wastewater1

Lifetime Imaging3

Evanescent3

Biological1

Vitro1

Emit1

Probes1

Spectral1

Methods3

Visualization2

Forensics1

Instrumentation2

Demonstrate1

Typically2

Wide-field5

Phenomenon2

SAMPLING1

Drawn1

Analysis4

Light4

Diagnosis1

Organic2

Examination1