Ficoll peripheral blood mononuclear. Medical search. Frequent questions

Peripheral blood mononuclear cells (PBMC) were extracted using a Ficoll gradient. (nih.gov)
Peripheral blood mononuclear cells (PBMC) are harvested from the blood sample using a Ficoll-Paque® density gradient. (wikipedia.org)
Analysis of S6 (pS244) in activated human peripheral blood mononuclear cells (PBMC). (bdbiosciences.com)
PBMC were isolated by density gradient centrifugation (Ficoll-Paque™ PLUS, Cat. (bdbiosciences.com)
Processing blood for serum, plasma, and peripheral blood mononuclear cells (PBMC). (usc.edu)
We analyzed the expression of PD1 and PD-L1 using qRT-PCR in RT4 bladder cancer (BCa) epithelial cells co-cultivated with peripheral blood mononuclear cells (PBMC) after treatment with BCG. (biomedcentral.com)

Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque gradient from the blood samples collected at days 0, 7, 14, 28, and final day of transplantation. (magiran.com)
Used cell isolation techniques to purify PBMCs from blood (Ficoll), select monocytes and CD4 T cells, differentiate and harvest dendritic cells and macrophages. (livecareer.com)
The aim of this research was to study the effects of diagnostic X-rays on peripheral blood mononuclear cells (PBMCs) and CD34 + /CD133 + populations in healthy volunteers in vitro and determine the ratio of CD34 + /CD133 + in PBMCs. (tci-thaijo.org)
The PBMCs were isolated by ficoll-centrifugation technique. (tci-thaijo.org)
We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)

Plasma and circulating peripheral blood mononuclear cells were isolated using Ficoll density centrifugation. (deepdyve.com)
Mononuclear leukocytes from Ficoll density centrifugation were found to support PV replication. (nih.gov)

Therefore, we developed a novel formula to improve the expansion of peripheral γδ T cells from healthy donors. (nature.com)
γδ T cells, specifically the Vγ9Vδ2 subset, which is the dominate subset among γδ T cells in human peripheral blood, recognize target cells in a human leukocyte antigen (HLA)-independent manner. (nature.com)
Specific white blood cells, called monocytes, are tasked with ingesting foreign material and become activated during certain inflammatory events. (wikipedia.org)
These activated monocytes come in contact with antibody-sensitized red blood cells (RBC) and may or may not exhibit phagocytosis (ingestion) and destroy the donor red blood cells. (wikipedia.org)
A clinically significant antibody is an antibody that is capable of causing in vitro hemolysis or a decreased survival of transfused donor red blood cells. (wikipedia.org)
This is an immune mediated reaction where recipient antibodies attack donor red blood cell antigen(s), causing hemolysis of donor cells. (wikipedia.org)
Antibodies to high prevalence red cell antigens can sometimes mask the detection of clinically significant alloantibodies because the corresponding antigen is present on most, if not all, of the screening red blood cells used to detect these antibodies possess the antigen. (wikipedia.org)
Flow cytometry was used to investigate the percentage of Th17 and Th1 cells in peripheral blood mononuclear cells. (spandidos-publications.com)
The results revealed that the prevalence of Th17 cells in patients with LSCC was elevated in their primary tumors, as well as in peripheral blood, compared with that in healthy controls. (spandidos-publications.com)
The present study revealed that patients with LSCC have elevated levels of Th17 cells in their primary tumors and peripheral blood compared with those in healthy controls. (spandidos-publications.com)
In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell line dependent on either GM-CSF or interleukin-3 (IL-3). (wiley.com)
Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells. (tci-thaijo.org)
Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting. (tci-thaijo.org)
Characteristics of peripheral blood stem cells: 2D-gel electrophoresis and kinetic parameter of exocytosis. (tci-thaijo.org)
In one embodiment stem cells are administered in order to protect the endothelium from apoptosis and to preserve the blood brain barrier. (therapeuticsolutionsint.com)
To determine safety and efficacy of intravenously administered allogeneic JadiCell umbilical cord blood mesenchymal stem cells in patients with moderate-to-severe COPD. (therapeuticsolutionsint.com)
There was no death in mice received different concentrations of nanohydrogel through oral administration and less than 5% hemolysis was found in red blood cells incubated with PMAA nanohydrogels. (bvsalud.org)
p75NTR, the specific receptor for proNGF, is the NGF receptor most expressed in mononuclear cells of patients with JIA, while TrkA is the prevalent receptor in healthy donors. (bmj.com)
proNGF effects depend on its binding to p75NTR, as inhibition of p75NTR with neutralising antibodies or LM11A-31 abolished proNGF-induced production of IL-6 in patients' mononuclear cells, while inhibition of TrkA did not. (bmj.com)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
In order to determine whether any primary human blood cells have the ability to replicate poliovirus (PV), peripheral blood cell components were isolated and analyzed for their cell surface expression of the poliovirus receptor (PVR). (nih.gov)
PVR cell surface expression was detected on most mononuclear cells expressing CD14, a marker for mononuclear phagocytes. (nih.gov)
The finding of PVR on mononuclear phagocytes and the ability of primary human blood cells to support PV replication in the absence of cultivation has implications for both the normal and pathogenic role of PVR. (nih.gov)
Mononuclear cells were separated from peripheral blood using a Ficoll gradient for ex vivo and in vitro immunophenotyping. (fiocruz.br)
We previously showed that the presence of NY-ESO-1-reactive and/or Melan-A-reactive T cells in the blood correlated with prolonged overall survival (OS) of patients with melanoma with a heterogeneous treatment background. (bmj.com)

The clinical significance of an alloantibody depends on its ability to cause a decrease in donor red blood cell survival. (wikipedia.org)

Dans la présente étude, les taux de production des interleukines 12 et 13 (IL-12 et IL-13) ont été mesurés à l'aide de la méthode ELISA dans les surnageants de culture de cellules mononucléées de sang périphérique stimulées par des mitogènes chez 30 cas de bêta-thalassémie non splénectomisés présentant une surcharge martiale ainsi que chez 20 individus en bonne santé appariés pour l'âge et le sexe. (who.int)
Plasma from peripheral blood was collected to measure tumor necrosis factor-a (TNF-α) and interleukin-8 (IL-8) by ELISA and nitrite (NO3) using a Griess assay. (bvsalud.org)

Exposure to antigens can occur through blood transfusion, stem cell/bone marrow transplant, and pregnancy. (wikipedia.org)
Ficoll-hypaque blood/bone marrow specimens. (usc.edu)

Blood banking is a concentration within a clinical laboratory that analyzes specimens from potential transfusion recipients and provides compatible blood products to the healthcare team in charge of that patient's care. (wikipedia.org)

Células mononucleares foram separadas do sangue periférico em gradiente de ficoll visando a imunofenotipagem ex vivo e in vitro. (fiocruz.br)

According to the International Society of Blood Transfusion (ISBT), 43 blood group systems containing hundreds of different red blood cell antigens have been described. (wikipedia.org)
Characteristics of clinically significant alloantibodies include: immunoglobulin G antibody subclass, reactivity at body temperature, and ability to cause red blood cell agglutination in the presence of anti-human globulin (AHG) in an indirect antiglobulin test. (wikipedia.org)
Endo lial progenitor cell (EPC), kind of cell which have stem cell characteristic, play important role in forming vessel in peripheral blood and have role to repair endo lial cell injury. (ub.ac.id)
Total mononuclear cell (MNCs) were isolated from peripheral blood by Ficoll. (ub.ac.id)
Peripheral blood cellular immunological changes were assessed by flow cytometry and transcript levels of BAFF , interferon (IFN)-induced and plasma cell-expressed genes were quantified by NanoString. (biomedcentral.com)
A number of the immunologic abnormalities seen in SARD, including changes in peripheral B (switched memory) and T (iNKT, T regulatory, activated memory T follicular helper) subsets and B cell activation, were also seen in asymptomatic ANA + subjects and those with UCTD. (biomedcentral.com)
In this study, we examined peripheral blood T and B cell populations and their activation in asymptomatic ANA + individuals together with patients with undifferentiated connective tissue disease (UCTD) and early SARD to determine whether the cellular immune characteristics found in SARD are distinct from those seen in asymptomatic ANA + individuals, most of whom will not progress to SARD. (biomedcentral.com)
Methods Peripheral blood T cell stimulation by NY-ESO-1 and Melan-A overlapping peptide libraries was assessed before and during ICB in two independent cohorts of a total of 111 patients with stage IV melanoma. (bmj.com)

Peripheral blood was obtained from female healthy donors from different ages. (nih.gov)
The following steps are performed in this assay: Anticoagulated blood is collected from normal, healthy individuals. (wikipedia.org)
Peripheral blood was obtained from another 36 patients with LSCC and from 16 healthy individuals, who served as controls. (spandidos-publications.com)

Development of new blood vessels is necessary for the effective tissue regeneration. (hindawi.com)

research shows that about 90% of the blood system of malignant tumors with at least the height of single gene methylation is closely related since this is a reversible process of genetic modification can be through to handle to restore the normal expression of gene methylation, to achieve the purpose of prevention and treatment of tumor [ 10 ]. (hindawi.com)

Therefore, we describe the effect of metformin in abdominal aortic aneurysm and at-risk patients on markers of inflammation, the driver of early abdominal aortic aneurysm formation and growth.MethodsPeripheral blood was collected from patients previously diagnosed with abdominal aortic aneurysm or presenting for their U.S. Preventive Task Force-recommended abdominal aortic aneurysm screening. (deepdyve.com)
To determine the circulating antibody to self-antigen response, a modified enzyme-linked immunosorbent assay was performed against antibodies to collagen type V and elastin fragments.ResultsPeripheral blood was isolated from 266 patients without diabetes mellitus (n=182), with diabetes mellitus not treated with metformin (n=34), and with diabetes mellitus actively taking metformin (n=50) from 2015 to 2017. (deepdyve.com)
Blood-based biomarker testing may help identify patients who are candidates for early antibody treatment. (questdiagnostics.com)
This study used blood samples from patients from different regions considered endemic for CL in Pernambuco. (researchsquare.com)
To compare the activity and function of NE in the peripheral blood from DS patients with and without PD, assisted at the Center for Dental Assistance to Patients with Special Needs affiliated with the School of Dentistry of Araçatuba, Brazil. (bvsalud.org)
Purified NE were collected from peripheral blood of 22 DS patients. (bvsalud.org)

After blood sampling, the frequency of Th17 and Tregs was evaluated using flow cytometry. (biomedcentral.com)

The assay is used to assess the potential for intravascular hemolysis when incompatible cellular blood products are transfused to the anemic patient. (wikipedia.org)

The monocyte monolayer assay (MMA) is used to determine the clinical significance of alloantibodies produced by blood transfusion recipients. (wikipedia.org)
There are many different varieties of abnormal reactions to blood transfusion. (wikipedia.org)

Bushmeat samples were obtained through a strategy specifically designed not to increase demand: women preparing and preserving the meat for subsequent sale and hunters already involved in the trade were asked for permission to sample blood and tissues from carcasses, which were then returned. (cdc.gov)
Methodology: Blood samples from 70 hospitalized PLWHA were collected within 24 hours of admission. (fiocruz.br)

Blood advances, 1(27), 2799-2816. (tci-thaijo.org)

Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. (biomedcentral.com)

With some exceptions, many non-ABO blood group system antigens require a sensitizing event to stimulate antibody production. (wikipedia.org)

Lastly, no differences in expression of collagen type V and elastin fragment antigen and/or antibodies were detected with metformin use in diabetics.ConclusionMetformin in diabetics at-risk for abdominal aortic aneurysm or diagnosed with abdominal aortic aneurysm does not seem to alter the peripheral inflammatory environment. (deepdyve.com)
T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. (biomedcentral.com)

Beyond the presence of naturally occurring antibodies (isohemagglutinins) to the ABO and Rh(D) blood group antigens, additional immune-stimulated antibodies are considered unexpected alloantibodies. (wikipedia.org)
When talking about the ABO blood group system, Landsteiner's Law states that if an individual possesses the A and/or B antigen, they will not form antibodies to these antigens. (wikipedia.org)

Migration of cultured peripheral blood mononuclear cells (PBMCs) from Medalists and age-matched controls were compared. (diabetesjournals.org)

Peripheral blood mononuclear cells were isolated using Ficoll-Hypaque density gradient from 14 subjects (2 with asthma, 11 with asthma and allergic rhinitis, and 1 with allergic rhinitis) and were stimulated by selected allergens (grass, tree, mite, and mold) in the absence or presence of 1 and 10 microM of zileuton. (nih.gov)
11. [Isolation of mononuclear cells from the bone marrow of patients with hemoblastoses using one-step ficoll-verographin density gradient separation]. (nih.gov)

A clinically significant antibody is an antibody that is capable of causing in vitro hemolysis or a decreased survival of transfused donor red blood cells. (wikipedia.org)
The aim of the present study was to investigate whether zileuton, a 5-lipoxygenase antagonist known to inhibit LTB4 production and recruitment of eosinophils/neutrophils in bronchoalveolar fluid, could affect the production of LTB4 and IL-8 by allergen-stimulated peripheral blood mononuclear cells in vitro from patients with asthma and/or allergic rhinitis. (nih.gov)
These cells were transferred to their offspring because after colostrum intake, the lymphocytes, isolated from the blood of the piglets, proliferated as well after in vitro stimulation. (biomedcentral.com)
SRLV got rapidly adapted in vitro to blood-derived macrophages and skin fibroblastic cells from red deer but not from fallow deer. (biomedcentral.com)
Mechanistic in vitro studies further unveiled the relevance of IFX in reducing NET release and normalizing the augmented inflammatory activities promoted by NETs in mononuclear cells. (biomedcentral.com)

This document describes the method of Ficoll separation used by Indiana Clinical and Translational Sciences Institute (CTSI) Specimen Storage Facility for the isolation of white cells or mononuclear white cells from ACD, heparin and EDTA anticoagulated blood. (nih.gov)
This report provides a method for extracting DNA using the PAXgene Blood DNA kit from granulocytes without purifying them from the separation medium. (cdc.gov)

The number of total lymphocytes per ml of blood in the neonatal subjects was approximately 4 million/ml. (nih.gov)
18. Immunological identification of peripheral-blood lymphocytes from patients with lymphoproliferative disorders in Nigeria. (nih.gov)

Specific white blood cells, called monocytes, are tasked with ingesting foreign material and become activated during certain inflammatory events. (wikipedia.org)
These activated monocytes come in contact with antibody-sensitized red blood cells (RBC) and may or may not exhibit phagocytosis (ingestion) and destroy the donor red blood cells. (wikipedia.org)

Peripheral blood was obtained from female healthy donors from different ages. (nih.gov)

Peripheral blood mononuclear cells were isolated and labeled with CFSE and proliferation rate was measured in exposure to candida antigen via flow cytometry. (ijfs.ir)
Parameters of intestinal Th2 inflammation were examined with our established procedures that included: levels of serum OVA-specific IgE antibody, serum histamine, numbers of mast cells, eosinophils and mononuclear cells in the lamina propria and antigen-specific Th2 cell proliferation. (rafinhibitors.com)

Beyond the presence of naturally occurring antibodies (isohemagglutinins) to the ABO and Rh(D) blood group antigens, additional immune-stimulated antibodies are considered unexpected alloantibodies. (wikipedia.org)
When talking about the ABO blood group system, Landsteiner's Law states that if an individual possesses the A and/or B antigen, they will not form antibodies to these antigens. (wikipedia.org)
According to the International Society of Blood Transfusion (ISBT), 43 blood group systems containing hundreds of different red blood cell antigens have been described. (wikipedia.org)
With some exceptions, many non-ABO blood group system antigens require a sensitizing event to stimulate antibody production. (wikipedia.org)
Antibodies to high prevalence red cell antigens can sometimes mask the detection of clinically significant alloantibodies because the corresponding antigen is present on most, if not all, of the screening red blood cells used to detect these antibodies possess the antigen. (wikipedia.org)
Induction of anergy is one means by which tolerance to antigens in peripheral tissues is induced, thus avoiding an autoimmune response [ 26 ]. (biomedcentral.com)

CPC levels were 15% higher in Medalists without CVD and nephropathy than in those affected, whereas EPC levels were significantly higher in those without peripheral vascular disease (PVD) than those with PVD. (diabetesjournals.org)
The mean GDS score and AA/EPA ratio, in whole blood and RBC membrane phospholipids, were significantly lower after 2 months supplementation with n-3 PUFA. (biomedcentral.com)

In blood of sows cytokine producing T-cells were increased upon M. hyopneumoniae vaccination. (biomedcentral.com)
In contrast, no M. hyopneumoniae -specific cytokine producing T-cells were found in blood of piglets from control sows. (biomedcentral.com)

They consist of several subsets characterised by adaptive and innate-like functions, with Vγ9Vδ2 being the largest subset in human peripheral blood. (nature.com)
Among several different types of human γδ T cells, Vγ9Vδ2 is the largest subset in peripheral blood. (nature.com)
Analysis of human mononuclear cells for a custom set of differentiation, activation, and exhaustion markers. (amc.nl)
Characteristics of clinically significant alloantibodies include: immunoglobulin G antibody subclass, reactivity at body temperature, and ability to cause red blood cell agglutination in the presence of anti-human globulin (AHG) in an indirect antiglobulin test. (wikipedia.org)
In human leukocyte antigen (HLA) mismatched transplants (4/6, 3/6), stem cell source was cord blood, resulting in grade 0-I aGVHD. (cancerhugs.com)
We cultured viable or heat-inactivated human umbilical cord MSCs with human peripheral blood mononuclear cells for 24 h and observed changes in monocyte surface marker expression and secretion profile. (biomedcentral.com)

Peripheral blood samples (N=8) were obtained for studies from patients diagnosed with AML at M.D. Anderson Malignancy Center (MDACC) following institutional recommendations. (healthyconnectionsinc.com)

20. Immunological characterization of non-Hodgkin's lymphoma: enrichment of neoplastic cells from lymphoid tissues and blood. (nih.gov)

BACKGROUND: Blood is a convenient sample and increasingly used for quantitative gene expression measurements with a variety of diseases including chronic fatigue syndrome (CFS). (cdc.gov)
Results of this study should facilitate large-scale molecular epidemiologic studies using blood RNA as the target of quantitative gene expression measurements. (cdc.gov)

The assay is used to assess the potential for intravascular hemolysis when incompatible cellular blood products are transfused to the anemic patient. (wikipedia.org)
The following steps are performed in this assay: Anticoagulated blood is collected from normal, healthy individuals. (wikipedia.org)
From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. (cdc.gov)
The TNF-α, IFN-γ and IL-17A production by different T-cell subsets in blood of sows, colostrum and blood of piglets was assessed using a recall assay. (biomedcentral.com)

The clinical significance of an alloantibody depends on its ability to cause a decrease in donor red blood cell survival. (wikipedia.org)

The n-3 PUFA status may be monitored by means of the determination of whole blood AA/EPA ratio. (biomedcentral.com)
Peripheral blood mononuclear cells were isolated from whole blood of 20 CVID and 10 healthy subjects. (biomedres.us)

Tumor necrosis factor α (TNF-α) production by freshly isolated peripheral blood mononuclear cells from 24 elderly persons and 16 young adults after 4-hour stimulation with lipopolysaccharide (LPS). (jamanetwork.com)

Several routine tests are performed including blood typing (determination of ABO/Rh status), antibody screening, serologic cross-matching, direct antiglobulin testing, and antibody identification. (wikipedia.org)

This connection stresses the need of studying the role of the peripheral immune system in neurological diseases. (biomedcentral.com)

A blood sample was obtained for ELISPOT within 7 days of microbiologic studies (including acid-fast smears [AFS] and mycobacterial culture). (cdc.gov)

Mitogens, inflammatory cytokines, and oxidative markers were measured in blood or urine. (diabetesjournals.org)

In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)

There was no death in mice received different concentrations of nanohydrogel through oral administration and less than 5% hemolysis was found in red blood cells incubated with PMAA nanohydrogels. (bvsalud.org)

Our results describe a validated procedure for the generation of genome-wide expression data from isolated lymphocyte sub-populations obtained from newborn blood. (nih.gov)

Dans la présente étude, les taux de production des interleukines 12 et 13 (IL-12 et IL-13) ont été mesurés à l'aide de la méthode ELISA dans les surnageants de culture de cellules mononucléées de sang périphérique stimulées par des mitogènes chez 30 cas de bêta-thalassémie non splénectomisés présentant une surcharge martiale ainsi que chez 20 individus en bonne santé appariés pour l'âge et le sexe. (who.int)

Anatomy14

Diseases2

Chemicals and Drugs12

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes3

PBMC6

PBMCs5

Centrifugation2

Cells27

Donor1

ELISA2

Bone2

Specimens1

Vitro1

Cell8

Healthy3

Tissue1

Tumor1

Patients6

Th171

Cellular1

Transfusion2

Samples2

Advances1

Primary1

Stimulate1

Expression2

Antibodies2

Cultured peripheral blood mononuclear cells1

Density2

Vitro5

Separation2

Lymphocytes2

Monocytes2

Donors1

Proliferation2

Antigens6

Significantly2

Cytokine2

Human6

Institutional1

Tissues1

Quantitative2

Assay4

Clinical1

Whole blood2

Elderly1

Antibody1

Immune1

Sample1

Inflammatory1

Study1

Hemolysis1

Populations1

Production1