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  • density-gradient
  • To determine the optimal conditions for an accurate analysis of chemokine receptors, we compared the expression of CCR2, CCR4, CCR5, CCR6, CXCR3 and CXCR4 on different leukocyte subsets using whole blood (WB) plus erythrocyte lysis and density gradient isolation (Ficoll). (nih.gov)
  • cells
  • Migration capacity of monocytes from WB and Ficoll PBMC towards CCL2.WB diluited 1∶5 in medium (100 µl per well) or Ficoll PBMC (2×106 cells/ml per well) were subjected to a 4 h-chemotaxis assay towards CCL2 or fMLP (n = 5). (nih.gov)
  • PBMC
  • After being centrifuged, the following layers will be visible in the column, from top to bottom: plasma and other constituents, mono-nuclear cells (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be present in pellet form. (wikidot.com)
  • Note that some red blood cell trapping (presence of erythrocytes & granulocytes) may occur in the PBMC or Ficoll-Paque layer. (wikidot.com)
  • Migration capacity of monocytes from WB and Ficoll PBMC towards CCL2.WB diluited 1∶5 in medium (100 µl per well) or Ficoll PBMC (2×106 cells/ml per well) were subjected to a 4 h-chemotaxis assay towards CCL2 or fMLP (n = 5). (nih.gov)
  • Diffusion
  • Diffusion of anionic Ficoll was significantly reduced compared with that of neutral Ficoll across the GFB for molecules of a(e) similar to 20-35 angstrom, while there were no charge effects for Ficoll of a(e) similar to 35-80 angstrom. (lu.se)
  • article{f2dfbdb6-d312-4d69-9be1-867ed28f34dd, abstract = {Axelsson J, Sverrisson K, Rippe A, Fissell W, Rippe B. Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo. (lu.se)
  • neutral
  • The CM-Ficoll used, however, showed a larger Stokes-Einstein radius (a(e)) than neutral Ficoll, and it was proposed that the introduction of negative charges in the Ficoll molecule had made it more flexible and permeable. (lu.se)
  • different
  • Regardless of different soluble factors or cell interactions that could take place in WB and MNC 4 h culture, the down-regulation of monocyte CCR2 expression by Ficoll may be associated with a decreased ability of monocytes to migrate towards CCL2. (nih.gov)